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    Faris S. Elbanna
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    This document provides an outline of topics to be covered in a practical microbiology course. The topics include: 1. Microscopic slides showing various bacteria, fungi, and staining techniques. 2. Examples of commonly used culture media including blood agar, chocolate agar, MacConkey's agar, and TCBS agar. 3. Examples of biochemical tests performed on bacteria, including coagulase testing, oxidase testing, and identification of Enterobacteriaceae. 4. Serological testing techniques like the Wassermann test and Widal test. 5. Examples of instruments used in microbiology like bacteriological loops and anaerobic jars.

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    This document provides an outline of topics to be covered in a practical microbiology course. The topics include: 1. Microscopic slides showing various bacteria, fungi, and staining techniques. 2. Examples of commonly used culture media including blood agar, chocolate agar, MacConkey's agar, and TCBS agar. 3. Examples of biochemical tests performed on bacteria, including coagulase testing, oxidase testing, and identification of Enterobacteriaceae. 4. Serological testing techniques like the Wassermann test and Widal test. 5. Examples of instruments used in microbiology like bacteriological loops and anaerobic jars.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    13 views133 pages

    Practical Slide

    Uploaded by

    Faris S. Elbanna
    This document provides an outline of topics to be covered in a practical microbiology course. The topics include: 1. Microscopic slides showing various bacteria, fungi, and staining techniques. 2. Examples of commonly used culture media including blood agar, chocolate agar, MacConkey's agar, and TCBS agar. 3. Examples of biochemical tests performed on bacteria, including coagulase testing, oxidase testing, and identification of Enterobacteriaceae. 4. Serological testing techniques like the Wassermann test and Widal test. 5. Examples of instruments used in microbiology like bacteriological loops and anaerobic jars.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 133

    Practical microbiology 2011

    : . (01 ) . demonstration ,,,,,,,,, ./

    Practical microbiology 2011


    1. 2. 3. 4. 5. Microscopic slides Media Biochemical reactions Serological tests Instruments

    1.Microscopic slides

    A- bacteria

    Gram negative bacilli

    Streptococcus Pyogenes in sputum

    Staphylococci in pus

    Pneumococci In sputum

    Anthracoids

    Neisseria Gonorrhea in urethral discharge

    Acid fast bacilli By Zeihl Neelsen stain

    Acid fast bacilli By Zeihl Neelsen stain

    Anthracoids and candida by Gram stain

    Candida and Gram negative bacilli

    Candida and staphylococci

    Staphylococci and anthracoids

    Diphtheroids

    Staphylococci In culture

    B- fungi

    Candida by lactophenol blue

    Aspergillus by lactophenol blue

    Germ tube test

    Septated hyphae

    Non septated hyphae

    Non septated hyphae

    Penicillium

    Candida by Gram stain

    2- Media

    Blood agar

    Beta haemolysis

    Beta haemolysis

    Alpha haemolysis

    Optochin resistant alpha haemolytic colonies (strept. Viridans)

    Optochin sensitive alpha haemolytic colonies (strept. pneomoniae)

    Chocolate agar

    MacConkeys agar

    CLED agar

    Nutrient agar

    Novobiocin resistant Staph saprophyticus

    Novobiocin sensitive Staph epidermidis

    Others

    Robertsons cooked meat media

    Slide culture technique

    Peptone water

    Nutrient broth

    SDA

    TCBS

    Vibrio cholera colonies on TCBS

    3- Biochemical reactions

    Coagulase

    coagulase positive

    Coagulase negative

    Enterobacteriacea

    Neisseria

    Salmonella

    BR of Salmonella paratyphi

    BR of Salmonella typhi

    Shigella

    Oxidase

    4- Seorological tests

    Wassermann test

    Wasserman test negative

    Wasserman test mild positive

    Wasserman test moderate positive

    Wasserman test strong positive

    Wasserman test antigen anticomplementary

    Wasserman test antibody anticomplementary

    Widal test

    5- Instruments

    Bacteriological loop

    Anaerobic Jar

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