Jabatan Sains Perikanan dan Akuakultur Fakulti Agroteknologi dan Sains Makanan Universiti Malaysia Terengganu Bioteknologi Akuakultur

AQU3601/SBA3503 Laboratory 11 BIOINFORMATICS ANALYSIS >AQU3601A_M13F

TGCATCTAGATTCAGCGCCCTTCTATCAACTACAAACATGGAGCATATAGTTGGTTCAG CAAGGAGGCTGACTGAACCAACAGGTCCAGTAACAATGATTTATAGCCGTCAGGCGGAAG GCTTTTCCTTAATAACATTAGCAATAGCCAGCTTCGCAGAGACAGGAAGGGTGTGTCGGG GGAAACAACATAACAAAGAGCGGATGAATGGAAGGATGAAGGAGAGAAAGGGCTGATCAC TATACGTGGCTGACTGACAATCTGAGGAGGAAGGGCCTGGAATGAGGCCTTGGAAACTAC CACTTGATAACACACTGTTCGTCGGGTGCCACATATAACCCGACAACACACTCACACAGG ATGGAGAAACAGTGGTCTGTGGGTTGCTCCAACCTCAACAGTGAAATGGAAAGTGAATAA TTGCATTGCTGTTCTTTGAAAAGGGAAGGGCCTGAATCGGATCCCGGGCCCGTCGACTGC AGAGGCCTGCATGCAAGCTTTCCCTATAGTGAGTCGTATTAGAGCTTGGCGTAATCATGG TCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCC GGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCG TTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATC GGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACT GACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTA ATACGGTTATCCACAGAATCAGGGGATACGCAGGAAAGAACATGTGAGCAAAGGTCAGCA AAAGNCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTCCATAGCTCCGCCCCCTTGA CGAGCATCACNAANCGACGCTCAAGTCANAGNTGCGAAACCGACNGACTATTAAGATACA GCGTTCCCCTTGANCCTCCCTCGGNNNNTCTCCNGTCCGACCTGGCNNNNNNNNCNNGTC GCNNCTCNNGANNGNCTTCN

>AQU3601A_M13F
CAGCGCCCTTCTATCAACTACAAACATGGAGCATATAGTTGGTTCAG CAAGGAGGCTGACTGAACCAACAGGTCCAGTAACAATGATTTATAGCCGTCAGGCGGAAG GCTTTTCCTTAATAACATTAGCAATAGCCAGCTTCGCAGAGACAGGAAGGGTGTGTCGGG GGAAACAACATAACAAAGAGCGGATGAATGGAAGGATGAAGGAGAGAAAGGGCTGATCAC TATACGTGGCTGACTGACAATCTGAGGAGGAAGGGCCTGGAATGAGGCCTTGGAAACTAC CACTTGATAACACACTGTTCGTCGGGTGCCACATATAACCCGACAACACACTCACACAGG ATGGAGAAACAGTGGTCTGTGGGTTGCTCCAACCTCAACAGTGAAATGGAAAGTGAATAA TTGCATTGCTGTTCTTTGAAAAGGGAAGGGCCTGA

Result

Nucleotide Sequence (442 letters)

Results for:

What's this?

Your BLAST job specified more than one input sequence. This box lets you choose which input sequence to show BLAST results for. Query ID lcl|8477 lcl|8477 Description None Molecule type nucleic acid Query Length 442 Database Name nr Description All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS,environmental samples or phase 0, 1 or 2 HTGS sequences) See details Program BLASTN 2.2.25+ Citation Blast search databases information Database Description Posted Date Reference Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14.

No significant similarity found

>AQU3601B
TGTATGAATATGAAATTAATTCTAATTGTTTATTTGATTTTGTCCTGCAGTTTCTTGAAA TCAGTCATGTCTTCTCCAAAAGGAATCGCTATTGGCATTGACCTGGGCACCACCTACTCC TGTGTGGGGGTGTTTCTGCATGGAAAAGTGGAGATCATCGCCAACGACCAGGGCAACAGA ACAACACCCAGCTATGATGCCTTCACAGACACAGAGAGGCTGATTGGAGACGCAGCTAAG AACCAGGTGGCCATGAATCCCAACAACACGGTGTTCGATGCCAAGAGGCTGATCGGCAGG AGGTTCGATGACCCTGTAGTGCAGTGTGACATGAAGCACTGGTCCTTCAAAGTCGTCAGT GATGGAGGAAAGCCATAAGTTGCAGTGGAACACTAAGGAGAAAACAAGACCTTTAATCCT GAAGAGATTTCCTCCATGGTCCTGGTGAAGATGAAGGAGATTGCAGAGGCTTATCTGGGG CAGAAGTTGACCATCGCAGTTATCACAGTTCCAGCCTATTTCAACGACTCCCAGAGACAG

GCCACTAAAGATGCTGGAGTCATCGCTGGACTTAAGCTGCTCCGCATCATCAACGAGCCC ACGGCTGCAGCCCATCCGTACGGCCTGGACAAAGGCAAATCCTCAGAGCGCAACGTCCTG ATCTTTCACCTGGGCGGAGGCACCTTCGACGTGTCCATCCTGACCATCGAAGACGGCATC TTTGAGGTGAAGGCCACCGCTGGAGACACTCATCTGGGCGGTGAGGACTTTGACAACCGC ATGGTGAACCACTTTGTGGAAGAGTTCAAGAGGAAGCACAAGAAGGACATCAGTCAGAAC AAGATGGCCCTGAGGAGGCTGTAAGGAGCATGTGAACGAGCCAAGAGGACGCTCTCGTCC AGCTCTCAGGCCAGCATTGAGATCGACTCTCTGTACGAGGGCATCGACTTCTACACGTCC ATCACCAGAGCTCGCTTCGAGGAGCTCTGCTCCGACCTCTTCATGGATACGCTTGATCCT GTGGAGAAAGCACTGAGGGACGCTAAGATGGACAAGGCTCAGATCCACGACATCGTGCTG GTTGGGGGCTCAACAAGAATCCCAAAGATCCAGAAGCTTCTGCAGGATTTCTTCAACGGC AGAGAACTGAACGAGAGCATTAACCCTGATGAAGCGGTGGCTTACGGTGCCGCGGTGCAG GCCGCCATCTTCAATGGGCGACACCTCTGGAAACGTGCAGGACCTGCTGCTGCTGGACGT GCCCCGCTGTCTCTGGGTATTGAGACCGCAGGTGGAGTCATGACGGCCCTCATCAAGCGC AACACAACCATCCCCACCAAACAGACCCAGACCTTCACCACCTACTCCGACAACCAGCCC GGTGTCCTGATCCAGGTGTTCGAGGGAGAAAGAGCCATGACCAAAGACAACAACCTGCTG GGCAAATTTGAGCTGACGGGAATTCCACCTGCGCCACGTGGCGTCCCGCAGATCGAAGTG ACCTTCGACATCGACGCCAACGGAATCCTAAATGTGTCGGCGGCGGACAAAAGCACCGGA AAACAGAACAAGATCACCATCACCAACGACAAGGGCAGGCTGAGCAAAGAGGAGATCGAG AGAATGGTGCAAGAGGCCGACATGTACAAAGCTGAAGACGATCTGCAGAGAGAGAAGATT TCTGCCAAAAACTCCCTGGATTCTTACGCCTTCAACTTGAAGAGCATTGTGGAAGACGAC AACCTGAAAGGCAAGATCAGCGAGGAGGACAAGAAGAGGGTTATTGAGAAGTGCAATGAA GCCGTGAGCTGGCTGGAGAACAACCAGCTGGGAGATAAAGAGGAGTACGAACATCAGCTG AAGGAGCTGGAGAAAGTCTGCAATCCAGTCATCTCCAAACTCTACCAGGGAGGGATGCCA GCTGGAGGATGTGGAGCTCAGGCACGGGGCGCATCAGGGCCAGCGCTCAGGGGCCCACCA TTGAAGAGGTGGATTAAAACTCCTCATGAACTGAACAAACTAGACAAAAAAACAATTCTT TGATTATTTTAAGATGACTTTATTTAAAGTCTTATTGCAC

Result

Nucleotide Sequence (2080 letters)

Results for:

What's this?

Your BLAST job specified more than one input sequence. This box lets you choose which input sequence to show BLAST results for. Query ID lcl|60777 lcl|60777 Description None Molecule type nucleic acid Query Length 2080 Database Name nr Description All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS,environmental samples or phase 0, 1 or 2 HTGS sequences) See details Program BLASTN 2.2.25+ Citation Blast search databases information

Database Description Posted Date Reference Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Other reports: Search Summary [Taxonomy reports] [Distance tree of results]

>AQU3601C
MAFSNVLSDSDVATALDGCKDAGTFDHKKFFSACGLSNKTSDDVKKAFAIIDQDKSGFIEEEELKLFL QNFKADARVLTDVETSTFLKAGDTDGDGKIGADEFTALVKP

>P02614 Graptemys geographica (Map turtle)

AMTDILSAKDIEAALTSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFIEEDELQLFLQ NFSSTARALTAAETKAFMAAGDTDGDGKIGVDEFQALVKA

>P19753 Gallus gallus (Chicken)

AITDILSAKDIESALSSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFIEEEELQLFLK NFSSSARVLTSAETKAFLAAGDTDGDGKIGVEEFQSLVKA

>P02617 Rana esculenta (Edible frog)

SITDIVSEKDIDAALESVKAAGSFNYKIFFQKVGLAGKSAADAKKVFEILDRDKSGFIEQDELGLFLQ NFRASARVLSDAETSAFLKAGDSDGDGKIGVEEFQALVKA

>P02619 Esox lucius (Northern pike)

SFAGLKDADVAAALAACSAADSFKHKEFFAKVGLASKSLDDVKKAFYVIDQDKSGFIEEDELKLFLQN FSPSARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKA

>X97825 Salmo salar (Atlantic salmon)

MSFAGLNDADVAAALAACTAADSFNHKAFFAKVGLASKSSDDVKKAFYVIDQDKSGFIEEDELKLFLQ NFSASARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKG

>P02618 Cyprinus carpio (Common carp)

AFAGVLNDADIAAALEACKAADSFNHKAFFAKVGLTSKSADDVKKAFAIIDQDKSGFIEEDELKLFLQ NFKADARALTDGETKTFLKAGDSDGDGKIGVDEFTALVKA

>P02621 Merlangius merlangus (Whiting)

AFAGILADADCAAAVKACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFIEEDELKLFLQ VFKAGARALTDAETKAFLKAGDSDGDGAIGVEEWVALVKA

>AY035584 Gadus morhua (codfish)

MAFAGILADADCAAAVKACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFIEEDELKLFL QVFKAGARALTDAETKAFLKAGDSDGDGAIGVDEWAVLVKA

>P56503 Merluccius bilinearis (Silver hake)

AFSGILADADVAAALKACEAADSFNYKAFFAKVGLTAKSADDIKKAFFVIDQDKSGFIEEDELKLFLQ VFSAGARALTDAETKAFLKAGDSDGDGAIGVDEWAALVKA

>P02620 Merluccius merluccius (European hake)

AFAGILADADITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFVEEDELKLFLQ NFSAGARALTDAETATFLKAGDSDGDGKIGVEEFAAMVKG

Result
MUSCLE (3.8) multiple sequence alignment AQU3601C P02620 P02619 X97825 P02618 P56503 P02621 AY035584 P02617 P02614 P19753 AQU3601C P02620 P02619 X97825 P02618 P56503 P02621 AY035584 P02617 P02614 P19753 MAFSNVLSDSDVATALDGCKDAGTFDHKKFFSACGLSNKTSDDVKKAFAIIDQDKSGFIE -AFAGILADADITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFVE -SFAG-LKDADVAAALAACSAADSFKHKEFFAKVGLASKSLDDVKKAFYVIDQDKSGFIE MSFAG-LNDADVAAALAACTAADSFNHKAFFAKVGLASKSSDDVKKAFYVIDQDKSGFIE -AFAGVLNDADIAAALEACKAADSFNHKAFFAKVGLTSKSADDVKKAFAIIDQDKSGFIE -AFSGILADADVAAALKACEAADSFNYKAFFAKVGLTAKSADDIKKAFFVIDQDKSGFIE -AFAGILADADCAAAVKACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFIE MAFAGILADADCAAAVKACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFIE -SITDIVSEKDIDAALESVKAAGSFNYKIFFQKVGLAGKSAADAKKVFEILDRDKSGFIE -AMTDILSAKDIEAALTSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFIE -AITDILSAKDIESALSSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFIE :::. : * :*: . :*.: ** ** *: : ** * .:*.***.*:* EEELKLFLQNFKADARVLTDVETSTFLKAGDTDGDGKIGADEFTALVKP EDELKLFLQNFSAGARALTDAETATFLKAGDSDGDGKIGVEEFAAMVKG EDELKLFLQNFSPSARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKA EDELKLFLQNFSASARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKG EDELKLFLQNFKADARALTDGETKTFLKAGDSDGDGKIGVDEFTALVKA EDELKLFLQVFSAGARALTDAETKAFLKAGDSDGDGAIGVDEWAALVKA EDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVEEWVALVKA EDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVDEWAVLVKA QDELGLFLQNFRASARVLSDAETSAFLKAGDSDGDGKIGVEEFQALVKA EDELQLFLQNFSSTARALTAAETKAFMAAGDTDGDGKIGVDEFQALVKA EEELQLFLKNFSSSARVLTSAETKAFLAAGDTDGDGKIGVEEFQSLVKA ::** ***: * . **.*: ** :*: **.**** **.:*: ::*

Exercise 4
>AQU3601C MAFSNVLSDSDVATALDGCKDAGTFDHKKFFSACGLSNKTSDDVKKAFAIIDQDKSGFIEEEELKLFL QNFKADARVLTDVETSTFLKAGDTDGDGKIGADEFTALVKP >P02614 Graptemys geographica (Map turtle) AMTDILSAKDIEAALTSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFIEEDELQLFLQ NFSSTARALTAAETKAFMAAGDTDGDGKIGVDEFQALVKA >P19753 Gallus gallus (Chicken) AITDILSAKDIESALSSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFIEEEELQLFLK NFSSSARVLTSAETKAFLAAGDTDGDGKIGVEEFQSLVKA >P02617 Rana esculenta (Edible frog) SITDIVSEKDIDAALESVKAAGSFNYKIFFQKVGLAGKSAADAKKVFEILDRDKSGFIEQDELGLFLQ NFRASARVLSDAETSAFLKAGDSDGDGKIGVEEFQALVKA >P02619 Esox lucius (Northern pike)

SFAGLKDADVAAALAACSAADSFKHKEFFAKVGLASKSLDDVKKAFYVIDQDKSGFIEEDELKLFLQN FSPSARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKA >X97825 Salmo salar (Atlantic salmon) MSFAGLNDADVAAALAACTAADSFNHKAFFAKVGLASKSSDDVKKAFYVIDQDKSGFIEEDELKLFLQ NFSASARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKG >P02618 Cyprinus carpio (Common carp) AFAGVLNDADIAAALEACKAADSFNHKAFFAKVGLTSKSADDVKKAFAIIDQDKSGFIEEDELKLFLQ NFKADARALTDGETKTFLKAGDSDGDGKIGVDEFTALVKA >P02621 Merlangius merlangus (Whiting) AFAGILADADCAAAVKACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFIEEDELKLFLQ VFKAGARALTDAETKAFLKAGDSDGDGAIGVEEWVALVKA >AY035584 Gadus morhua (codfish) MAFAGILADADCAAAVKACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFIEEDELKLFL QVFKAGARALTDAETKAFLKAGDSDGDGAIGVDEWAVLVKA >P56503 Merluccius bilinearis (Silver hake) AFSGILADADVAAALKACEAADSFNYKAFFAKVGLTAKSADDIKKAFFVIDQDKSGFIEEDELKLFLQ VFSAGARALTDAETKAFLKAGDSDGDGAIGVDEWAALVKA >P02620 Merluccius merluccius (European hake) AFAGILADADITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFVEEDELKLFLQ NFSAGARALTDAETATFLKAGDSDGDGKIGVEEFAAMVKG

Result

Alignment
Results Parameters
Name Value

User e-mail address Max. num of motifs Output Format Length threshold Power threshold Coincidence ratio Minimum letter frequence Windows width Max. number of shifts Min. homology ratio How to process motifs Motif frequences recalc Method 100 GeneBee 7 3.5 0.005 0.6 7 5 0.0 s-s N g

Gap penalty (in SD units) Register size

3 1

Matrices DAYHOFF
DAYHOFF AMINO ACIDS DISTANCE MATRIX A C D E F G H I K L M A 12 C 8 22 D 10 5 14 E 10 5 13 14 F 6 6 4 5 19 G 11 7 11 10 5 15 H 9 7 11 11 8 8 16 I 9 8 8 8 11 7 8 15 K 9 5 10 10 5 8 10 8 15 L 8 4 6 7 12 6 8 12 7 16 M 9 5 7 8 10 7 8 12 10 14 16 N 10 6 12 11 6 10 12 8 11 7 8 P 11 7 9 9 5 9 10 8 9 7 8 Q 10 5 12 12 5 9 13 8 11 8 9 R 8 6 9 9 6 7 12 8 13 7 10 S 11 10 10 10 7 11 9 9 10 7 8 T 11 8 10 10 7 10 9 10 10 8 9 V 10 8 8 8 9 9 8 14 8 12 12 W 4 2 3 3 10 3 7 5 7 8 6 Y 7 10 6 6 17 5 10 9 6 9 8 N P Q R S T V W Y

12 9 11 10 11 10 8 6 8

16 10 14 10 11 16 11 9 10 12 10 9 9 11 13 9 8 8 9 10 14 4 5 12 8 5 4 27 5 6 6 7 7 8 10 20

Sequences

>AQU3601C MAFSNVLSDSDVATALDGCKDAGTFDHKKFFSACGLSNKTSDDVKKAFAIIDQDKSGFIEEEELKLFLQNFKADAR >P02614 Graptemys geographica (Map turtle) AMTDILSAKDIEAALTSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFIEEDELQLFLQNFSSTARA >P19753 Gallus gallus (Chicken) AITDILSAKDIESALSSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFIEEEELQLFLKNFSSSARV >P02617 Rana esculenta (Edible frog) SITDIVSEKDIDAALESVKAAGSFNYKIFFQKVGLAGKSAADAKKVFEILDRDKSGFIEQDELGLFLQNFRASARV >P02619 Esox lucius (Northern pike) SFAGLKDADVAAALAACSAADSFKHKEFFAKVGLASKSLDDVKKAFYVIDQDKSGFIEEDELKLFLQNFSPSARAL >X97825 Salmo salar (Atlantic salmon) MSFAGLNDADVAAALAACTAADSFNHKAFFAKVGLASKSSDDVKKAFYVIDQDKSGFIEEDELKLFLQNFSASARA >P02618 Cyprinus carpio (Common carp) AFAGVLNDADIAAALEACKAADSFNHKAFFAKVGLTSKSADDVKKAFAIIDQDKSGFIEEDELKLFLQNFKADARA >P02621 Merlangius merlangus (Whiting) AFAGILADADCAAAVKACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFIEEDELKLFLQVFKAGARA >AY035584 Gadus morhua (codfish) MAFAGILADADCAAAVKACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFIEEDELKLFLQVFKAGAR >P56503 Merluccius bilinearis (Silver hake) AFSGILADADVAAALKACEAADSFNYKAFFAKVGLTAKSADDIKKAFFVIDQDKSGFIEEDELKLFLQVFSAGARA >P02620 Merluccius merluccius (European hake) AFAGILADADITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFVEEDELKLFLQNFSAGARA

166.70

REFINED ALIGNMENT Power

Homology percent

72.6

The meaning of signs at the top of the alignment is following: ' ' - the average weight of column pair exchanges is less than weight matrix mean value '.' - is less than mean value plus one SD '+' - is less than mean value plus two SD '*' - is more than mean value plus two SD

.+++++*.++*+.+*+.+*++++**+**.**+++**.+*+.**+**+*.+ +********* AQU3601C ( 1) MAFSNVLSDSDVATALDGCKDAGTFDHKKFFSACGLSNKTSDDVKKAFAIIDQDKSGFIE P02614 ( 1) -AMTDILSAKDIEAALTSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFIE P19753 ( 1) -AITDILSAKDIESALSSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFIE P02617 ( 1) -SITDIVSEKDIDAALESVKAAGSFNYKIFFQKVGLAGKSAADAKKVFEILDRDKSGFIE P02619 ( 1) -SFAGLKDADVAAALAACSAADSFKHKEFFAKVGLASKSLDDVKKAFYVIDQDKSGFIE X97825 ( 1) MSFAGLNDADVAAALAACTAADSFNHKAFFAKVGLASKSSDDVKKAFYVIDQDKSGFIE P02618 ( 1) -AFAGVLNDADIAAALEACKAADSFNHKAFFAKVGLTSKSADDVKKAFAIIDQDKSGFIE P02621 ( 1) -AFAGILADADCAAAVKACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFIE AY035584 ( 1) MAFAGILADADCAAAVKACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFIE P56503 ( 1) -AFSGILADADVAAALKACEAADSFNYKAFFAKVGLTAKSADDIKKAFFVIDQDKSGFIE P02620 ( 1) -AFAGILADADITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFVE ****+****+*++.**+***+**++**++**+****.******.++**+ EEELKLFLQNFKADARVLTDVETSTFLKAGDTDGDGKIGADEFTALVKP EDELQLFLQNFSSTARALTAAETKAFMAAGDTDGDGKIGVDEFQALVKA EEELQLFLKNFSSSARVLTSAETKAFLAAGDTDGDGKIGVEEFQSLVKA QDELGLFLQNFRASARVLSDAETSAFLKAGDSDGDGKIGVEEFQALVKA EDELKLFLQNFSPSARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKA EDELKLFLQNFSASARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKG EDELKLFLQNFKADARALTDGETKTFLKAGDSDGDGKIGVDEFTALVKA EDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVEEWVALVKA EDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVDEWAVLVKA EDELKLFLQVFSAGARALTDAETKAFLKAGDSDGDGAIGVDEWAALVKA EDELKLFLQNFSAGARALTDAETATFLKAGDSDGDGKIGVEEFAAMVKG

AQU3601C P02614 P19753 P02617 P02619 X97825 P02618 P02621 AY035584 P56503 P02620

( ( ( ( ( ( ( ( ( ( (

61) 60) 60) 60) 59) 60) 60) 60) 61) 60) 60)

Graphical Phylogenetic Tree

Put mouse over a tree type below. Cluster Algorithm: Slanted Phylogram Topological Algorithm: Slanted Phylogram

PHYLOGENETIC

TREE

CLUSTER ALGORITHM
0.329441 __________________________________________________________________ | |___________________________________________________________ | | | |____________________ | | |___________________________________________ | | |_____________________________________ | | | |_______________ | | |_______________________ | |____________________________________________________ |_________________________________________________________________ | |______________________________

AQU3601C P02619 X97825 P02618 P02621 AY035584 P56503 P02620 P02614 P19753

|________________________________________________________ P02617 * The phylogenetic tree in Phylip format ((AQU3601C:0.297431,(((P02619:0.096495,X97825:0.096495):0.121203, (P02618:0.183291, ((P02621:0.071942,AY035584:0.071942):0.043485,P56503:0.115427):0.067864):0. 034407):0.042123,P02620:0.259821):0.037609):0.032010, ((P02614:0.148432,P19753:0.148432):0.134943,P02617:0.283375):0.046066);

TOPOLOGICAL ALGORITHM
______________________________________ P02621 | | | | | |________________________ AY035584 | | | | |_____________________ P56503 | | | |__________________ P02618 | | | |_______________ P02619 | | | |_______________________ X97825 | | |______________________________ P02620 | |__________________ P02614 | | |_______________________________ P19753 | |________________________________ P02617 |_______________ AQU3601C * The phylogenetic tree in Phylip format (((((((P02621:0.001000,AY035584:0.130893):0.035098,P56503:0.115437):0.05417 1,P02618:0.099074):0.001000, (P02619:0.001000,X97825:0.130008):0.079662):0.015088,P02620:0.163358):0.012 180, ((P02614:0.001000,P19753:0.174217):0.057914,P02617:0.176868):0.040975):0.08 7435,AQU3601C:0.087435); DISTANCE MATRIX 3 4 5

11 1 AQU3601C 0.304 2 P02614 0.310 3 P19753 0.351 4 P02617 0.313 5 P02619 0.273 6 X97825 0.273 7 P02618 0.231 8 P02621 0.268 9 AY035584 0.270 10 P56503 0.255

10

0.000 0.339 0.322 0.343 0.332 0.317 0.213 0.298 0.298 0.303 0.339 0.000 0.148 0.276 0.308 0.297 0.267 0.312 0.312 0.277 0.322 0.148 0.000 0.291 0.336 0.326 0.297 0.339 0.348 0.307 0.343 0.276 0.291 0.000 0.371 0.354 0.292 0.336 0.343 0.324 0.332 0.308 0.336 0.371 0.000 0.096 0.215 0.269 0.255 0.222 0.317 0.297 0.326 0.354 0.096 0.000 0.186 0.258 0.245 0.204 0.213 0.267 0.297 0.292 0.215 0.186 0.000 0.196 0.201 0.168 0.298 0.312 0.339 0.336 0.269 0.258 0.196 0.000 0.072 0.124 0.298 0.312 0.348 0.343 0.255 0.245 0.201 0.072 0.000 0.106 0.303 0.277 0.307 0.324 0.222 0.204 0.168 0.124 0.106 0.000

11 P02620 0.000

0.304 0.310 0.351 0.313 0.273 0.273 0.231 0.268 0.270 0.255

DRAFT SOURCE ALIGNMENT Power 166.70

Homology percent

72.6

The meaning of signs at the top of the alignment is following: ' ' - the average weight of column pair exchanges is less than weight matrix mean value '.' - is less than mean value plus one SD '+' - is less than mean value plus two SD '*' - is more than mean value plus two SD

.+++++*.++*+.+*+.+*++++**+**.**+++**.+*+.**+**+*.+ +********* AQU3601C ( 1) MAFSNVLSDSDVATALDGCKDAGTFDHKKFFSACGLSNKTSDDVKKAFAIIDQDKSGFIE P02614 ( 1) -AMTDILSAKDIEAALTSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFIE P19753 ( 1) -AITDILSAKDIESALSSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFIE P02617 ( 1) -SITDIVSEKDIDAALESVKAAGSFNYKIFFQKVGLAGKSAADAKKVFEILDRDKSGFIE P02619 ( 1) -SFAGLKDADVAAALAACSAADSFKHKEFFAKVGLASKSLDDVKKAFYVIDQDKSGFIE X97825 ( 1) MSFAGLNDADVAAALAACTAADSFNHKAFFAKVGLASKSSDDVKKAFYVIDQDKSGFIE P02618 ( 1) -AFAGVLNDADIAAALEACKAADSFNHKAFFAKVGLTSKSADDVKKAFAIIDQDKSGFIE P02621 ( 1) -AFAGILADADCAAAVKACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFIE AY035584 ( 1) MAFAGILADADCAAAVKACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFIE P56503 ( 1) -AFSGILADADVAAALKACEAADSFNYKAFFAKVGLTAKSADDIKKAFFVIDQDKSGFIE P02620 ( 1) -AFAGILADADITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFVE ****+****+*++.**+***+**++**++**+****.******.++**+ EEELKLFLQNFKADARVLTDVETSTFLKAGDTDGDGKIGADEFTALVKP EDELQLFLQNFSSTARALTAAETKAFMAAGDTDGDGKIGVDEFQALVKA EEELQLFLKNFSSSARVLTSAETKAFLAAGDTDGDGKIGVEEFQSLVKA QDELGLFLQNFRASARVLSDAETSAFLKAGDSDGDGKIGVEEFQALVKA EDELKLFLQNFSPSARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKA EDELKLFLQNFSASARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKG EDELKLFLQNFKADARALTDGETKTFLKAGDSDGDGKIGVDEFTALVKA EDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVEEWVALVKA EDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVDEWAVLVKA

AQU3601C P02614 P19753 P02617 P02619 X97825 P02618 P02621 AY035584

( ( ( ( ( ( ( ( (

61) 60) 60) 60) 59) 60) 60) 60) 61)

P56503 P02620

( (

60) EDELKLFLQVFSAGARALTDAETKAFLKAGDSDGDGAIGVDEWAALVKA 60) EDELKLFLQNFSAGARALTDAETATFLKAGDSDGDGKIGVEEFAAMVKG

10 BEST LOCAL ALIGNMENTS (SUPERMOTIFS)

The meaning of signs at the top of the alignment is following: ' ' - the average weight of column pair exchanges is less than weight matrix mean value '.' - is less than mean value plus one SD '+' - is less than mean value plus two SD '*' - is more than mean value plus two SD

LOCAL SUPERMOTIF number 1, power

50.05

.++..+*.++*+++*+.+*++++**+**.**+++**.+*+.*****+*. +********* AQU3601C ( 1) -MAFSNVLSDSDVATALDGCKDAGTFDHKKFFSACGLSNKTSDDVKKAFAIIDQDKSGFI P02614 ( 1) --AMTDILSAKDIEAALTSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFI P19753 ( 1) --AITDILSAKDIESALSSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFI P02619 ( 1) -SFAG-LKDADVAAALAACSAADSFKHKEFFAKVGLASKSLDDVKKAFYVIDQDKSGFI X97825 ( 1) mSFAG-LNDADVAAALAACTAADSFNHKAFFAKVGLASKSSDDVKKAFYVIDQDKSGFI P02618 ( 1) --AFAGVLNDADIAAALEACKAADSFNHKAFFAKVGLTSKSADDVKKAFAIIDQDKSGFI P02621 ( 1) --AFAGILADADCAAAVKACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFI AY035584 ( 1) -MAFAGILADADCAAAVKACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFI P56503 ( 1) --AFSGILADADVAAALKACEAADSFNYKAFFAKVGLTAKSADDIKKAFFVIDQDKSGFI P02620 ( 1) --AFAGILADADITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFV **********+*++.**+***+**++**++**+****.**+***.++**+ EEEELKLFLQNFKADARVLTDVETSTFLKAGDTDGDGKIGADEFTALVKp EEDELQLFLQNFSSTARALTAAETKAFMAAGDTDGDGKIGVDEFQALVKA EEEELQLFLKNFSSSARVLTSAETKAFLAAGDTDGDGKIGVEEFQSLVKA EEDELKLFLQNFSPSARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKA EEDELKLFLQNFSASARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKG EEDELKLFLQNFKADARALTDGETKTFLKAGDSDGDGKIGVDEFTALVKA

AQU3601C P02614 P19753 P02619 X97825 P02618

( ( ( ( ( (

60) 59) 59) 58) 59) 59)

P02621 AY035584 P56503 P02620

( ( ( (

59) 60) 59) 59)

EEDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVEEWVALVKA EEDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVDEWAVLVKA EEDELKLFLQVFSAGARALTDAETKAFLKAGDSDGDGAIGVDEWAALVKA EEDELKLFLQNFSAGARALTDAETATFLKAGDSDGDGKIGVEEFAAMVKG

LOCAL SUPERMOTIF number 2, power

22.20

.*+++**+++*+.+*+.+*++++**+**.**+++**.+*+++*+**+*.+ +********* AQU3601C ( 1) mAFSNVLSDSDVATALDGCKDAGTFDHKKFFSACGLSNKTSDDVKKAFAIIDQDKSGFIE P02614 ( 1) -AMTDILSAKDIEAALTSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFIE P19753 ( 1) -AITDILSAKDIESALSSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFIE P02617 ( 1) -SITDIVSEKDIDAALESVKAAGSFNYKIFFQKVGLAGKSAADAKKVFEILDRDKSGFIE P02618 ( 1) -afagVLNDADIAAALEACKAADSFNHKAFFAKVGLTSKSADDVKKAFAIIDQDKSGFIE P02621 ( 1) -afagILADADCAAAVKACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFIE AY035584 ( 1) mafagILADADCAAAVKACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFIE P56503 ( 1) -AFSGILADADVAAALKACEAADSFNYKAFFAKVGLTAKSADDIKKAFFVIDQDKSGFIE P02620 ( 1) -afagILADADITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFVE ****+****+*++.**+**++**++**+***+****+**+***.+***+ EEELKLFLQNFKADARVLTDVETSTFLKAGDTDGDGKIGADEFTALVKp EDELQLFLQNFSSTARALTAAETKAFMAAGDTDGDGKIGVDEFQALVKA EEELQLFLKNFSSSARVLTSAETKAFLAAGDTDGDGKIGVEEFQSLVKA QDELGLFLQNFRASARVLSDAETSAFLKAGDSDGDGKIGVEEFQALVKA EDELKLFLQNFKADARALTDGETKTFLKAGDSDGDGKIGVDEFTALVKA EDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVEEWVALVKA EDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVDEWAVLVKA EDELKLFLQVFSAGARALTDAETKAFLKAGDSDGDGAIGVDEWAALVKA EDELKLFLQNFSAGARALTDAETATFLKAGDSDGDGKIGVEEFAAMVKg

AQU3601C P02614 P19753 P02617 P02618 P02621 AY035584 P56503 P02620

( ( ( ( ( ( ( ( (

61) 60) 60) 60) 60) 60) 61) 60) 60)

LOCAL SUPERMOTIF number 3, power

....+.*.**.***.++.**+**+** **.*****+**..*+**+*.*+*+*******+ P02617 ( 1) sitdiVSEKDIDAALESVKAAGSFNYKIFFQKVGLAGKSAADAKKVFEILDRDKSGFIEQ P02619 ( 1) -sfagLKDADVAAALAACSAADSFKHKEFFAKVGLASKSLDDVKKAFYVIDQDKSGFIEE X97825 ( 1) msfagLNDADVAAALAACTAADSFNHKAFFAKVGLASKSSDDVKKAFYVIDQDKSGFIEE

18.31

P02617 P02619 X97825

( ( (

***.******..***+*+****+***..**+****.******.*+**+ 61) DELGLFLQNFRASARVLSDAETSAFLKAGDSDGDGKIGVEEFQALVKA 60) DELKLFLQNFSPSARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKA 61) DELKLFLQNFSASARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKg

LOCAL SUPERMOTIF number 4, power

6.20

...+...++.+.+..+*+++*+++++.*+...+.+*...*+.+*.* ..+++++ ++++*. P02614 ( 2) mtdilsakdieaaltSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFIEED P19753 ( 2) itdilsakdiesalsSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFIEEE X97825 ( 21) adsfnhkaffAKVGLASKSSDDVKKAFYVIDQDKSGFIEEDELKLFLQNFSASARALTDA P02621 ( 2) fagiladadcAAAVKACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFIEED AY035584 ( 3) fagiladadcAAAVKACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFIEED P56503 ( 21) adsfnykaffakvglTAKSADDIKKAFFVIDQDKSGFIEEDELKLFLQVFSAGARALTDA *+++**+.+.+.++.++.+*+.*++.+*+.++++..+. ELQLFLQNFSSTARALTAAETKAFMAAGdtdgdgkigv ELQLFLKNFSSSARVLTSAETKAFLAAGdtdgdgkigv ETKAFLADGDKDGDGMIGVDEFAAMIKG---------ELKLFLQVFKAGARALTDAETKAFLKAGdsdgdgaigv ELKLFLQVFKAGARALTDAETKAFLKAGdsdgdgaigv ETKAFLKAGDSDGDGAIGVDEWAALVKA----------

P02614 P19753 X97825 P02621 AY035584 P56503

( ( ( ( ( (

62) 62) 81) 62) 63) 81)

LOCAL SUPERMOTIF number 5, power

5.82

. . .. . .. +.............. .++++*++ +++.*+ AQU3601C ( 1) -----------------------------mafsnvlsdsdvataldgcKDAGTFDHKKFF P02614 ( 1) ------------------------------amtdilsakdieaaltSCQAADSFNYKSFF P19753 ( 1) ------------------------------aitdilsakdiesalsSCQAADSFNYKSFF X97825 ( 26) hkaffakvglAS-----------------------------------KSSDDVKKAFYV P02618 ( 7) ndadiaaaleAC-----------------------------------KAADSFNHKAFF P02621 ( 1) ------------------------------afagiladadcaaavkacEAADSFSYKAFF AY035584 ( 1) ----------mafagiladadcaaavkaceaaesfsykaffakcglSGKSADDIKKAFFV

...+++++..*+.+*.*...+++++++++*.*+++**+.+.++++.++..*+.+ ++.+*+ AQU3601C ( 32) SACGLSNKTSDDVKKAFAIIDQDKSGFIEEEELKLFLQNFKADARVLTDVETSTFLKAGd P02614 ( 31) SKVGLKGKSTDQVKKIFGILDQDKSGFIEEDELQLFLQNFSSTARALTAAETKAFMAAGd P19753 ( 31) STVGLSSKTPDQIKKVFGILDQDKSGFIEEEELQLFLKNFSSSARVLTSAETKAFLAAGd X97825 ( 50) IDQDKSGFIEEDELKLFLQNFSASARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKGP02618 ( 31) AKVGLTSKSADDVKKAFAIIDQDKSGFIEEDELKLFLQNFKADARALtdgetktflkagd P02621 ( 31) AKCGLSGKSADDIKKAFVFIDQDKSGFIEEDELKLFLQVFKAGARALTDAETKAFLkagd AY035584 ( 51) IDQDKSGFIEEDELKLFLQVFKAGARALTDAETKAFLKAGDSDGDGAIGVDEWAVLVKA.++++.++. tdgdgkiga tdgdgkigv tdgdgkigv --------sdgdgkigv sdgdgaigv ---------

AQU3601C P02614 P19753 X97825 P02618 P02621 AY035584

( ( ( ( ( ( (

92) 91) 91) 109) 91) 91) 110)

LOCAL SUPERMOTIF number 6, power

5.79

*.*.++**********+**++***.*++.+*++*++**++*++***+****+ +*+**+.+ AQU3601C ( 46) kafaiidqdkSGFIEEEELKLFLQNFKAdarvltdvetstflkAGDTDGDGKigadefta P02614 ( 45) kifgildqdkSGFIEEDELQLFLQNFSSTARALTAAETKAFMAAGDTDGDGKigvdefqa P19753 ( 45) kvfgildqdkSGFIEEEELQLFLKNFSSSARVLTSAETKAFLAAGDTDGDGKigveefqs P02617 ( 1) --------siTDIVSEKDIDAALESVKAAGSFNYKIFFQKVGLAGKSAADAKkvfeildr P02618 ( 45) kafaiidqdkSGFIEEDELKLFLQNFKAdaraltdgetktflkAGDSDGDGKigvdefta P02621 ( 45) kafvfidqdkSGFIEEDELKLFLQVFKAGAraltdaetkaflkAGDSDGDGaigveewva AY035584 ( 46) kaffvidqdkSGFIEEDELKLFLQVFKAGAraltdaetkaflkAGDSDGDGaigvdewav P56503 ( 45) kaffvidqdkSGFIEEDELKLFLQVFSAGAraltdaetkaflkAGDSDGDGaigvdewaa P02620 ( 45) kvfgiidqdkSDFVEEDELKLFLQNFSAGAraltdaetatflkAGDSDGDGKigveefaa ++ lv lv lv dk lv lv

AQU3601C P02614 P19753 P02617 P02618 P02621

( ( ( ( ( (

106) 105) 105) 53) 105) 105)

AY035584 P56503 P02620

( ( (

106) lv 105) lv 105) mv

LOCAL SUPERMOTIF number 7, power

5.72

.. . . . +...+.+.++.. +*. .... P02619 ( 1) ------------------------------sfaglkdADVAAALAACSAADSFKHKEFFA X97825 ( 1) ----------msfaglndadvaaalaactaadsfnhkAFFAKVGLASKSSDDVKKAFYVI P02621 ( 1) ---------aF----------------------------AGILADADCAAAVK-----AY035584 ( 1) --------maF----------------------------AGILADADCAAAVK-----P02620 ( 1) afagiladadI----------------------------TAALAACKAEGSFKhgefft ....+ ....*. .. .+. ....+. ..*+..+.*. ........... +...... .. P02619 ( 31) KVGLASKSLDDVKKAFYVIDQDKSGFIEEDELKLFLQNFSPSARALTDAETKAFLADGd X97825 ( 51) DQDKSGFIEEDELKLFLQNFSASARALTDAETKAFLADGDKDGDGMIGVDEFAAMIKGP02621 ( 17) ----ACEAADSFSYKAFFAKCGLSGKSADDIKKAFVFIDQDKSGFIEEDELKLFLQVFK AY035584 ( 18) ----ACEAAESFSYKAFFAKCGLSGKSADDIKKAFFVIDQDKSGFIEEDELKLFLQVFK P02620 ( 32) kiglkgKSAADIKKVFGIIDQDKSDFVEEDELKLFLQNFSAGARALTDAETATFLKAGDS .........+..+.............. kdgdgmigvdefaamika----------------------------------AGARALTDAETKAFLKAgdsdgdgaig AGARALTDAETKAFLKAgdsdgdgaig DGDGKIGVEEFAAMVKG----------

P02619 X97825 P02621 AY035584 P02620

( ( ( ( (

90) 109) 72) 73) 92)

LOCAL SUPERMOTIF number 8, power

5.27

. .. . . ..... .. .. ................ ......... ....+. AQU3601C ( 1) ----------------------------mafsnvlsdsdvataldgcKDAGTFDHKKFFS P02614 ( 1) -----------------------------amtdilsakdieaaltSCQAADSFNYKSFFS P19753 ( 1) -----------------------------aitdilsakdiesalsSCQAADSFNYKSFFS

P02619 ( 25) hkeffakvglASKSLDDVKKAFYVIDQDK------------------------------P02618 ( 7) ndadiaaaleACKAADSFNHKAFFAKVGL------------------------------P02621 ( 1) ----------afagiladadcaaavkaceaadsfsykaffakcglSGKSADDIKKAFVFI ...++...*+.+*.*...+++++++++*.*+++**+++.++++.++..*+.++ +.+++. AQU3601C ( 33) ACGLSNKTSDDVKKAFAIIDQDKSGFIEEEELKLFLQNFKADARVLTDVEtstflkagdt P02614 ( 32) KVGLkgkstdqvkkifgildqdksgfieedelqlflqnfsstaraltaaeTKAFMAAGdt P19753 ( 32) TVGLssktpdqikkvfgildqdksgfieeeelqlflknfsssarvltsaeTKAFLAAGdt P02619 ( 54) ----SGFIEEDELKLFLQNFSPSARALTDAETKAFLADGDKDGDGMIGVDefaamika-P02618 ( 36) ----TSKSADDVKKAFAIIDQDKSGFIEEDELKLFLQNFKADARALtdgetktflkagds P02621 ( 51) DQDKsgfieedelklflqvfkagaraltdaetkaflkagdsdgdgaigveEWVALVKA--

LOCAL SUPERMOTIF number 9, power

5.23

. ............+....+....+. ................+..... +.+ +. .... P02614 ( 1) amtdilsakdIEAALTSCQAADSFNYKSFFSKVGLKGKSTDQVKKIFGILDQDKSGFIEE P19753 ( 1) aitdilsakdIESALSSCQAADSFNYKSFFSTVGLSSKTPDQIKKVFGILDQDKSGFIEE P02619 ( 1) -------------------sfAGLKDAD----------VAAALAACSAADSFKHKEFFA X97825 ( 1) ---------msfAGLNDADVA--AALAactaadsfnhkaffakvglaskssddvkkafy P02618 ( 21) adsfnhkaffaKVGLTSKSADDVKKAFAIIDQDKSGFIEEDELKLFLQNFKADARALTDG P56503 ( 1) ---------aFSGILADADVAAALKACEAADSFNYKAFFAKVGLTAKSADDIKKAFFVID P02620 ( 1) afagiladadITAALAACKAEGSFKHGEFFTKIGLKGKSAADIKKVFGIIDQDKSDFVEE .. . .....+....... .+......+.+........ DELQLFLQNFSSTARaltaaetkafmaaGDTDGDGKIG EELQLFLKNFSSSARvltsaetkaflaaGDTDGDGKIG KVGLASKSLDDVKKAfyvidqdksgfieEDELKLFLQN vidqdksgfieedelKLFLQNFSASARAltdaetkafl ETKTFLKAGDSDGDGKIGVDEFTALVKA---------QDKSGFIEEDELKLFlqvfsagaraltdAETKAFLKAG DELKLFLQNFSAGARaltdaetatflkaGDSDGDGKIG

P02614 P19753 P02619 X97825 P02618 P56503 P02620

( ( ( ( ( ( (

61) 61) 31) 49) 81) 52) 61)

LOCAL SUPERMOTIF number 10, power

**++.++..+**+***+**..+*+++*+++ ----amtdilSAKDIEAALTscqaadsfny ffakvgltskSADDVKKAFAiidqdksgfi ffakcglsgkSADDIKKAFVfidqdksgfi ffakcglsgkSADDIKKAFfvidqdksgfi ffakvgltakSADDIKKAFfvidqdksgfi

5.08

P02614 P02618 P02621 AY035584 P56503

( ( ( ( (

1) 29) 29) 30) 29)

Exercise 5 Nucleotide for growth hormone, teleost 30 sequences Example : 1. Oncorhynchus mykiss insulin-like growth factor II (igf2), mRNA 1,148 bp linear mRNA Accession: NM_001124697.1 GI: 185135792 GenBank FASTA Graphics Related Sequences 2. Danio rerio somatolactin alpha (smtla), mRNA 804 bp linear mRNA Accession: NM_001037706.1 GI: 83415155 GenBank FASTA Graphics Related Sequences 3. Carassius auratus growth-hormone releasing hormone-like peptide receptor mRNA, complete cds 2,224 bp linear mRNA Accession: AF048819.1 GI: 3098566 GenBank FASTA Graphics

Protein for growth hormone teleost 35 sequences Example: growth hormone [Takifugu rubripes] 196 aa protein Accession: AAC60105.1 GI: 1932757 GenPept FASTA Graphics Related Sequences Identical Proteins growth hormone [Poecilia reticulata] 83 aa protein Accession: AAC60106.1 GI: 1932759 GenPept FASTA Graphics Related Sequences growth hormone [Colisa lalia] 83 aa protein Accession: AAC60104.1 GI: 1932755 GenPept FASTA Graphics Related Sequences

pubMed for growth hormone teleost 326 sequences Example: