BICD 120 Fall 2008 TA: Abraham Tang

Section: Wednesday 6-6:50PM, York 3000A abraham.h.tang@gmail.com

OH: by appointment http://plantbio08.wordpress.com
Handout week 7
Lecture 12
• Auxin receptor

o
o Cloned mutated genes
 Ubiquitin-mediated proteolysis


Psalm 103:15 (NKJV)

As for man, his days are like grass;
As a flower of the field, so he flourishes.
BICD 120 Fall 2008 TA: Abraham Tang
Section: Wednesday 6-6:50PM, York 3000A abraham.h.tang@gmail.com
OH: by appointment http://plantbio08.wordpress.com
o

o TIR1-axr
 SCF protein binds AUX proteins


 If you add auxin, ARF becomes free

 If have SCF mutant… ARF will NOT be released, KEY POINT
 Mutant situation, aux never binds to SCF-Tir1
 Wildtype: binds in presence of auxin
 TIR1 refers to a wildtype gene that acts as a SCF complex
component that normally degrades AUX proteins in presence of
auxin (wildtype TIR1 protein)
 Mutant = tir1-, the SCFTIR1 is inactive and can no longer bind to
the aux protein
• If you can’t bind aux, you can’t respond to auxin
 Left side, wildtype, right side mutant
• Mutant is auxin RESISTANT

Psalm 103:15 (NKJV)

As for man, his days are like grass;
As a flower of the field, so he flourishes.
BICD 120 Fall 2008 TA: Abraham Tang
Section: Wednesday 6-6:50PM, York 3000A abraham.h.tang@gmail.com
OH: by appointment http://plantbio08.wordpress.com
• Cyokinin: (+) promotes growth and cell division


o Actions:


o Gibberellin (GA) (+)
•

Psalm 103:15 (NKJV)

As for man, his days are like grass;
As a flower of the field, so he flourishes.
BICD 120 Fall 2008 TA: Abraham Tang
Section: Wednesday 6-6:50PM, York 3000A abraham.h.tang@gmail.com
OH: by appointment http://plantbio08.wordpress.com
• Reproductive growth


o 2 types of mutants
 Biosynthesis mutant
 Receptor mutant
• ABA- abscisic acid (-)  keeps seed dormant by blocking GA responses


Psalm 103:15 (NKJV)

As for man, his days are like grass;
As a flower of the field, so he flourishes.