DESZING OF GREY COTTON FABRIC BY ENZYMB METHOD

Aim

To Desize the gray or muslin fabric for the removal of starch by enzyme method.

Properties of Enzymes : enzymes are bio-catalyst of organic origin, secreted by living organisms. Chemically enzymes are proteins of high molecular weight. Enzymes are highly specific in their action on substrates. For example amylase act only on starch not
on cellilose.

Materials required : Grey cotton fabric, burette, pipette, electronic balance, measuring
cylinders etc,

Chemicals used and their functions: Amvlase Enzvme : It Hydrolyses(Removes) the starch present in the fabric. Common salt

: 'io trwrtlr;,rl 4e en>ll*'\L a'uh'cn 4 ff*

0.5

Sfalct\'[-rtr.1.-

Recipe:
Amylase enzyme: 2 Salt: 3 wetting Agent 4 Temperature: 5 pH:

3 Gpl

- I Gpl - 7.s

Gpl

60-700c
6.5

6M:L
7 Time

lz20 I hours

Desizing Procedure:

First the fabric is weighed in electronic balance and the weight is noted.
Set the Desizing bath with required amount of water and enzyme .Wet the fabric and add into the bath stirrer well.

Add the common salt. Increase the temperature of the bath 60 work for 2 hours

-70 " C gradually, and

Care to be taken so that the temperature should not more than 70o C, because the enzymes are become in active above 70o C.

After 2 hours remove the fabric from the desizing bath wash cold water thoroughly, and dry it.

Calculation:
Wight of the material

for example 10 gms

20

M: L : 1:20

:10X

- 200 ml Amylase to be taken 0.5 to I Gpl

For 1000 ml or llitter, 0.5 or 1 ml of amylase required :200x I For 200 ml amylase quantity will be
1000

:0.2 ml
Common Salt to be taken 3 GPL For 1000 ml 10 gms of common salt required For 200 ml common salt quantity will be

:200X3

:
Fabric Swatches Before Desizing

LOOO 0.6 Gms

After Desizing

Loss in weight

Weight of the fabric before process - Weight of the fabric After the
Process

'--gms

Yo age

of weight loss

loss in weieht weight of the fabric before processing

100

Result : Change in Fabric feel is noted , weight loss due to the process is calculated In percentage.