GLYCOLYSIS & TCA CYCLE ENZYMES & EC REFERENCE NUMBERS

GLUCOSE
Hexokinase 2.7.1.1 ADP Glucose-6-P 5.3.1.9 Glucose-6-phosphate isomerase Fructose-6- P ATP 2.7.1.11 6-Phosphofructokinase
ATP

Fructose-1,6-bis-P 4.1.2.13 Fructose-bis-phosphate aldolase 3-P-Triose 2NAD+ 1.2.1.12 Glyceraldehyde 3-P-dehydrogenase NADH+H + 1,3-bis-P-Glycerate 2ADP 2.7.2.3 Phosphoglycerate kinase ATP 3-P-Glycerate 5.4.2.1 Phosphoglycerate mutase 2-P-Glycerate 4.2.1.11 Phosphopyruvate hydratase H2 O P-enolpyruvate ADP 2.7.1.40 Pyruvate kinase

ADP

PYRUVATE
PYRUVATE 1.2.4.1 ACETYL-CoA Succinyl-CoA hydrolase Oxaloacetate

ATP

IUBMB ENZYME COMMISSION (EC) CLASSIFICATION NUMBERS 1... 2... 3... 4... 5... 6... OXIDOREDUCTASES TRANSFERASES HYDROLASES LYASES ISOMERASES LIGASES

Pyruvate dehydrogenase (lipoamide)

NADH+H+

4.1.3.7 Citrate synthase

Citrate

4.2.1.3 Aconitate hydratase NADH+H+ 1.1.1.37 Malate dehydrogenase Malate Isocitrate Fumarate 4.2.1.2 1.1.1.41 Isoocitrate dehydrogenase H 2O hydratase NADH+H+ Fumarate 2-Oxoglutarate 1.3.5.1 Succinate dehydrogenase (ubiquinone)
Succinate Succinyl-CoA
GDP

UQH2

1.2.4.2 Oxoglutarate dehydrogenase (lipoamide)

NADH+H+

6.2.1.4 Succinate-CoA ligase (GTP-forming)

GTP

ENZYMES
1.1.1.37 1.1.1.41 1.2.1.12 1.2.4.1 1.2.4.2 1.3.5.1 2.7.1.1 2.7.1.11 2.7.1.40 2.7.2.3 Malate dehydrogenase Isocitrate dehydrogenase Glyceraldehyde-3-P dehydrogenase Pyruvate dehydrogenase (lipoamide) Oxoglutarate dehydrogenase (lipoamide) Succinate dehydrogenase (ubiquinone) Hexokinase 6-Phosphofructokinase Pyruvate kinase Phosphoglycerate kinase 3.1.2.3 4.1.2.13 4.1.3.7 4.2.1.2 4.2.1.3 4.2.1.11 5.3.1.9 5.4.2.1 6.2.1.4 Succinyl-CoA hydrolase Fructose-bis-phosphate aldolase Citrate synthase Fumarate hydratase Aconitate hydratase Phosphopyruvate hydratase Glucose-6-phosphate isomerase Phosphoglycerate mutase Succinate-CoA ligase

The IUBMB Enzyme Commission (EC) Numbers are all 4-figure numbers, the first of which denotes one of ONLY 6 CLASSES of enzyme as shown above. These identify the biochemical nature of every reaction and are worth memorising

Designed by Donald Nicholson

c 2002 IUBMB