GLUCOSE AGAR ready-to-use

INTENDED USE Glucose agar allows the demonstration of glucose fermentation (with or without gas production) as an identification test for Bacillus cereus, Enterobacteriaceae, or Pseudomonas in the context of the following standards : NF EN ISO 7932 (1998) for the enumeration of Bacillus cereus, NF ISO 21528 (2004) for the detection and enumeration of Enterobacteriaceae, or ISO/WD 13720 (2000) for the enumeration of Pseudomonas spp.

PRINCIPLES - The nutritivity of the medium is due to its content of casein peptone, yeast extract and glucose. - Fermentation of glucose is demonstrated through acidification, which turns the pH indicator (bromocresol purple) yellow. - Sodium chloride helps to maintain osmotic equilibrium.

PREPARATION - Before use, it is recommended to melt the agar tubes in a boiling water bath for the minimum amount of time to insure total liquefaction, and to resolidify in the correct position. - Do not repeat this operation more than once.

INSTRUCTIONS FOR USE - From a suspect colony taken from a selective isolation media and purified on Nutrient agar, inoculate the butt of the tube by stabbing in the center of the tube. - It is required to use pure cultures taken from isolated, distinct colonies in order to avoid cross reactions that render identification impossible. - Incubate at 37C for 24 hours, caps slightly unscrewed, in order to favor gas exchange.

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RESULTS The medium allows a differentiation and demonstration of glucose fermentation : Purple butt : Negative glucose fermentation. Yellow butt : Positive glucose fermentation.

Typical reactions are presented in the following table : SPECIES Salmonella Shigella dysenteriae Shigella flexneri Shigella sonnei Shigella boydii Proteus vulgaris Proteus mirabilis Proteus morganii Proteus rettgeri Serratia marcescens Enterobacter hafniae Enterobacter aerogenes Enterobacter cloacae Escherichia coli Citrobacter freundii Klebsiella pneumoniae Bacillus cereus Alcaligenes faecalis Pseudomonas aeruginosa (1) Yersinia enterocolitica
(1)

Glucose fermentation + + + + + + + + + + + + + + + + + -

a few strains of Pseudomonas may develop a yellow coloration, due to oxidation of glucose, on the surface of the agar.

TYPICAL COMPOSITION (can be adjusted to obtain optimal performance) For 1 liter of medium : - Tryptone .........................................................................................10.0 g - Yeast extract ....................................................................................1.5 g - Sodium chloride ...............................................................................5.0 g - Glucose ..........................................................................................10.0 g - Bromocresol purple .....................................................................15.0 mg - Bacteriological agar .......................................................................12.0 g pH of the ready-to-use medium at 25C : 7.0 0.2.

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QUALITY CONTROL Aspect, color : purple agar. Typical cultural response after 24 hours of incubation at 37C : Microorganisms Escherichia coli Salmonella Typhimurium Bacillus cereus Bacillus cereus Pseudomonas aeruginosa Pseudomonas aeruginosa ATCC 25922 ATCC 14028 ATCC 11178 ATCC 14579 CIP 82.118 ATCC 27853 Growth good, score 2 good, score 2 good, score 2 good, score 2 good, score 2 good, score 2 Glucose fermentation positive positive positive positive negative negative

STORAGE / SHELF LIFE - Store between 2-8C, shielded from light. - The expiration date is indicated on the label.

PACKAGING - 50 x 10 mL tubes

Code BM09908

BIBLIOGRAPHY
FIL provisoire 181. Dcembre 1998. Produits laitiers secs. Dnombrement de Bacillus cereus. Technique du nombre le plus probable. XP CEN ISO/TS 11133-2 (V 08-104-2). Janvier 2004. Microbiologie des aliments. Guide pour la prparation et la production des milieux de culture. Partie 2 : Guide gnral pour les essais de performance des milieux de culture. NF ISO 21528-1 (V 08-039-1). Dcembre 2004. Microbiologie des aliments. Mthodes horizontales pour la recherche et le dnombrement des Enterobacteriaceae. Partie 1 : Recherche et dnombrement laide de la technique NPP avec prenrichissement. NF ISO 21528-2 (V 08-039-2). Dcembre 2004. Microbiologie des aliments. Mthodes horizontales pour la recherche et le dnombrement des Enterobacteriaceae. Partie 2 : Mthode par comptage des colonies. ISO/CD 13720. Fvrier 2007. Viandes et produits base de viande. Dnombrement de Pseudomonas spp.

The information provided on the package takes precedence over the formulations or instructions described in this document. The information and specifications contained in this technical data sheet date from 2010-10-05. They are susceptible to modification at any time, without warning. Code document : BM099/A/2006-02 : 4.

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