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Biokar Diagnostics Rue des Quarante Mines ZAC de Ther Allonne B.P. 10245 F60002 Beauvais Cedex France
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NUTRIENT BROTH


INTENDED USE

Nutrient Broth is a general usage medium for a large variety of microorganisms without particular
nutritional requirements. Its formula complies with the directives of the French J .O. of August 8, 1972
for the detection of the intrinsic inhibitory capacity of cosmetic products.


PRINCIPLES

- Nutrient Broth is composed of a mixture of Tryptone and meat extract, which aids microbial growth.
- Sodium chloride is included to maintain osmotic equilibrium.


PREPARATION

- Dissolve 20.0 g of dehydrated medium (BK003) in 1 liter of distilled or deionized water.
- Stir slowly until complete dissolution.
- Dispense in tubes or flasks.
- Sterilize in an autoclave at 121C for 15 minutes.


INSTRUCTIONS FOR USE

- Inoculate the medium with purified cultures or with other type of mixed microflora inocula.
- Incubate at the required optimal temperature, aerobically or in a CO
2
-enriched atmosphere,
depending on the nature of the bacteria to be cultured.


RESULTS

Growth is shown by turbidity resulting from microbial multiplication.


TYPICAL COMPOSITION
(can be adjusted to obtain optimal performance)

For 1 liter of medium :
Tryptone...........................................................................................10.0 g
Meat extract........................................................................................5.0 g
Sodium chloride .................................................................................5.0 g

pH of the ready-to-use medium at 25C : 7.2 0.2.




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Biokar Diagnostics Rue des Quarante Mines ZAC de Ther Allonne B.P. 10245 F60002 Beauvais Cedex France
Tl : +33 (0)3 44 14 33 33 Fax : +33 (0)3 44 14 33 34 www.biokar-diagnostics.com


QUALITY CONTROL

- Dehydrated medium : cream powder, free-flowing and homogeneous.
- Prepared medium : amber solution, limpid.
- Typical culture response after 24 hours of incubation at 37C (inoculum 10
2
microorganisms) :

Microorganisms Growth
Escherichia coli ATCC

25922
Salmonella Typhimurium ATCC 14028
Enterococcus faecalis ATCC 29212
Staphylococcus aureus ATCC 25923
positive
positive
positive
positive


STORAGE / SHELF LIFE

Dehydrated medium : 2-30C.
- The expiration date is indicated on the label.
Prepared medium (benchmark value*) :
- Media in tubes or vials : 6 months at 2-8C.


PACKAGING Code

Dehydrated medium :
- 500 g bottle BK003HA


BIBLIOGRAPHY

J ournal Officiel du 8 Aot 1972. Mthode officielle danalyse bactriologique pour la recherche dun pouvoir inhibiteur
intrinsque.

XP CEN ISO/TS 11133-2 (V 08-104-2). J anvier 2004. Microbiologie des aliments. Guide pour la prparation et la production
des milieux de culture. Partie 2 : Guide gnral pour les essais de performance des milieux de culture.













*Benchmark value refers to the expected shelf life when prepared under standard laboratory conditions following manufacturers instructions. It is provided as a
guide only and no warranty, implied or otherwise is associated with this information.

The information provided on the package or instructions takes precedence over the formulations or instructions described in this document.
The information and specifications contained in this technical data sheet date from 2009-02-16.
They are susceptible to modification at any time, without warning.
Code document : BS028/A/2000-10 : 6.