Postdoctoral Researcher at

Leibniz Institute for the Analysis of

Biodiversity Change (LIB)
Museum Koenig (ZFMK)
Bonn
Germany
Francisco J. Ruiz-Ruano B F[dot]RuizRuano[at]leibniz-lib[dot]de
ORCID: http://orcid.org/0000-0002-5391-301X
Curriculum Vitae http://fjruizruano.wordpress.com

Education
November 2018 Accreditation (prerequisite) for application to postitions as PhD as-
sistant lecturer, PhD lecturer and Professor of private university in
Spain, ANECA.
October 2011- PhD in Fundamental and Systems Biology, Thesis (translated title):
July 2016 Nature and origin of the B chromosomes of Locusta migratoria,
directed by Camacho, J.P.M., Department of Genetics, University of Granada
(Spain).
October 2010- Master Degree in Genetics and Evolution, Final project (translated title):
September 2011 Evolution of the internal transcribed spacers of the rDNA 45S
in grasshoppers, directed by Camacho, J.P.M., Department of Genetics,
University of Granada, Spain.
October 2005- Graduate studies in Biology, University of Granada, Spain.
September 2010

Research experience
Current and previous positions
April 2023 - Postdoctoral researcher, Suh Group, Museum Koenig (ZFMK), Leibniz In-
present stitute for the Analysis of Biodiversity Change (LIB).
December 2022 - Postdoctoral researcher, Bartolomaeus Group, Institute of Evolutionary Bio-
March 2023 logy and Ecology, University of Bonn.
November 2022 Postdoctoral researcher, Suh Group, ZMB, School of Biological Sciences,
University of East Anglia.
January 2021 - Marie Skłodowska-Curie Actions - Individual European Fellowship,
October 2022 Suh Group, School of Biological Sciences, University of East Anglia.
January 2020 - Extension of Lawskis Foundation Postdoctoral Fellowship, Suh Group,
December 2020 Department of Organismal Biology - Systematic Biology, Uppsala University.
December 2019 Assistant professor (Declined), Department of Biology - Area of Genetics,
Autonomous University of Madrid.
January 2019 - Lawskis Foundation Postdoctoral Fellowship, Suh Group, Department of
December 2019 Ecology and Genetics, Uppsala University.
February 2018 - Postdoctoral Fellowship Associated to an I+D Project, Government
December 2018 of Spain, Evolutionary Genetics Group, Department of Genetics, University of
Granada.
February 2017 - Postdoctoral Fellowship Associated to an Excellence Project, Govern-
January 2018 ment of Andalusia, Evolutionary Genetics Group, Department of Genetics,
University of Granada.

Grants as main or only applicant

2021-2022 birdGRC - Evolution and function of the germline-restricted chro-
mosome in songbirds, University of East Anglia - Marie Curie Individual
Fellowship from European Commision REA (H2020-MSCA-IF-2019, 875732), PI:
Francisco J. Ruiz-Ruano, 203,852 EUR.
2020-2022 Decoding the genetic bases of mimicry in leaf-shaped katydids, Uppsala
University - The Royal Physiographic Society of Lund, PI: Francisco J. Ruiz-
Ruano, 80,000 SEK = 8,000 EUR.
2019-2021 Unraveling the functional significance of germline-restricted chromo-
somes, Uppsala University - The Royal Physiographic Society of Lund, PI:
Francisco J. Ruiz-Ruano, 85,000 SEK = 8,500 EUR.
2019-2020 The role of genomic dark matter in potato, Uppsala University - Lawski
Foundation (N2018-0007), PI: Francisco J. Ruiz-Ruano, 540,000 SEK = 54,000
EUR.

Grants as co-applicant
2021-2024 Evolutionary dynamics of germline-restricted chromosomes in song-
birds, Uppsala University - Project Grant of the Swedish Research Council
Vetenskapsrådet (VR) (2020-04436), PI: Alexander Suh. Role: Co-applicant,
3,265,200 SEK = 326,520 EUR.
2020-2021 Long-term evolution of germline-restricted chromosomes in songbirds,
Uppsala University - National Bioinformatics Infrastructure Sweden, PI: Alexan-
der Suh. Role: Co-applicant, 500 hours (+ 200 hours extension) of bioinformatic
support.

Grants as collaborator
2022-2025 Sexual selection and sex-differential transcriptome in red deer, Uni-
versity of Cordoba - Government of Spain (PID2021-127823NB-I00), PI: Juan
Carranza. Role: Collaborator, 100,000 EUR.
2021-2024 Análisis de la vulnerabilidad demográfica y genética de especies
centinela de cambio climático, University of Granada - Government of An-
dalusia (PY20_00018), PI: Manuel Jesús López Rodríguez. Role: Collaborator,
100,000 EUR.
2020-2023 The hybrid zone of Chorthippus parallelus: a window into the ge-
netic basis of speciation, Autonomous University of Madrid - Government of
Spain (PID2019-104952GB-I00), PIs: José L. Bella and David Buckley. Role:
Collaborator, 145,200 EUR.
 2020-2023 Structure, functions and evolution of meiotic chromosomes of birds,
Institute of Cytology and Genetics - Russian Science Foundation (20-64-46021),
PI: Anna Torgasheva. Role: Collaborator.
2016-2018 Transcriptomic changes provoked by a intragenomic parasite, Univer-
sity of Granada - Government of Spain (CGL2015-70750-P), PI: Juan Pedro M.
Camacho. Role: Collaborator, 143,385 EUR.
2011-2015 Nature, origin and expression of the B chromosomes of Locusta mi-
gratoria, University of Granada - Government of Andalusia (CGL2009-11917),
PI: Juan Pedro M. Camacho. Role: Collaborator, 208,247 EUR.
2010-2013 Unveiling the intercommunication between A and B chromosomes of
the grasshopper Eyprepocnemis plorans, University of Granada - Govern-
ment of Spain (P10-CVI-6649), PI: Josefa Cabrero. Role: Collaborator, 221,914
EUR.

Awards and scholarships

February 2014 International Mobility Grant for PhD students of the University of
- June 2014 Granada and CEI BioTic Granada, Brief stay in the Evolutionary Biology
Center, Wolf Lab, Department of Evolutionary Biology, Uppsala University.
December 2009 - Collaboration Grant, Government of Spain, Evolutionary Genetics Group,
June 2010 Department of Genetics, University of Granada.
May 2009 - May Introduction of Research Grant, University of Granada, Evolutionary
2010 Genetics Group, Department of Genetics, University of Granada.
December 2009 Grant to the best qualifications in the Biology Degree, University of
Granada.
July - JAE-Intro Grant, CSIC, Functional Genomics Group, Andalucian Develop-
September 2009 mental Biology Institute (CABD), CSIC.

Further research experience

October - Collaborative visit to the Max Plank Institute for Ornithology (Seew-
November 2022 iesen, Germany), Wolfgang Forstmeier Lab.
November - Collaborative visit to the Institute of Cytology and Genetics (Novos-
December 2021 ibirsk, Russia), Anna Torgasheva Lab.
December 2019 Collaborative visit to the São Paulo State University, UNESP (Rio
Claro and Bauru, Brazil), Diogo Cabral-de-Mello Lab and Fabio Porto-Foresti
Labs.
May 2019 Collaborative visit to the Max Plank Institute for Ornithology (Seew-
iesen, Germany), Wolfgang Forstmeier Lab.
February 2012 - Predoctoral Fellowship Associated to an Excellence Project, Govern-
January 2016 ment of Andalusia, Evolutionary Genetics Group, Department of Genetics,
University of Granada.
December 2011 Predoctoral Fellowship FPU, Government of Spain, Evolutionary Genet-
- January 2012 ics Group, Department of Genetics, University of Granada.
July - JAE-Intro Grant, CSIC, Functional Genomics Group. PIs: José Luis Gómez-
September 2009 Skarmeta and Jose Bessa, Andalucian Developmental Biology Institute (CABD),
CSIC.
February 2008 - Student intern, Evolutionary Genetics Group. PI: Juan Pedro M. Camacho,
September 2010 Department of Genetics, University of Granada.

Publication list
Articles in refereed journals (preprints and under review)
Preprint Rapid gene content turnover on the germline-restricted chromosome
in songbirds., Schlebusch, S.; Rídl, J.; Poignet, M.; Ruiz-Ruano, F.J.; Reif,
J.; Pačes, J.; Albrecht, T.; Suh, A.; Reifová, R., ResearchSquare, 1359388,
January 2023, (view at publisher).
Preprint The B chromosome of Pseudococcus viburni: a selfish chromosome
that exploits whole-genome meiotic drive, Vea, I.M.; de la Filia, A.G.;
Jaron, K.S.; Mongue, A.J.; Ruiz-Ruano, F.J.; Barlow, S.J.; Nelson, R.; Ross,
L., BioRxiv, 458195, August 2021, (view at publisher).
Preprint Evolutionary success of a parasitic B chromosome rests on gene con-
tent, Ruiz-Ruano, F.J.; Navarro-Dominguez, B.; López-León, M.D.; Cabrero,
J.; Camacho, J.P.M., BioRxiv, 683417, June 2019, (view at publisher).

Articles in refereed journals (published or in press)

2023 Tandem repeat DNA provides many cytological markers for hybrid
zone analysis in two subspecies of the grasshopper Chorthippus par-
allelus, Navarro-Dominguez, B.; Cabrero, J.; López-León, M.D.; Ruiz-Ruano,
F.J.; Pita, M.; Bella, J.L.; Camacho, J. P. M., Genes, 14:397, (view at pub-
lisher).
2022 In-depth Satellitome Analyses of 37 Drosophila species illuminates
repetitive DNA evolution in Drosophila genus, De Lima, L.G.; Ruiz-
Ruano, F.J., Genome Biology and Evolution, 14(5):evac064, (view at
publisher).
2022 Mendelian nightmares: The germline-restricted chromosome of song-
birds, Borodin, P.M.; Chen, A.; Forstmeier, W.; Fouché, S.; Malinovskaya, L.;
Pei, Y.; Reifová, R.; Ruiz-Ruano, F.J.; Schlebusch, S.A.; Sotelo-Muñoz, M.;
Torgasheva, A.; Vontzou, N.; Suh, A., Chromosome Research, 30:255–272,
(view at publisher).

2022 Satellitome comparison of two oedipodine grasshoppers highlights the

contingent nature of satellite DNA evolution, Camacho, J.P.M.; Cabrero,
J.; López-León, M.D.; Martín-Peciña, M.; Perfectti, F.; Garrido-Ramos, M.A.;
Ruiz-Ruano, F. J., BMC Biology, 20: 36, (view at publisher).
2022 Occasional paternal inheritance of the germline-restricted chromo-
some in songbirds, Pei, Y.; Forstmeier, W.; Ruiz-Ruano, F.J.; Mueller,
J.C.; Cabrero, J.; Camacho, J.P.M.; Alché, J.D.; Franke, A.; Hoeppner, M.;
Börno, S.; Gessara, I.; Hertel, M.; Teltscher, K.; Knief, U.; Suh, A.; Kempenaers,
B., PNAS 119: e2103960119, (view at publisher).
2021 Transposable element landscapes illuminate past evolutionary events
in the endangered fern Vandenboschia speciosa, Ruiz-Ruano, F.J.;
Navarro-Domínguez, B.; Camacho, J.P.M.; Garrido-Ramos, M.A., Genome
65(2): 95–103, (view at publisher).
2021 Out of patterns, the euchromatic B chromosome of the grasshopper
Abracris flavolineata is not enriched in high-copy repeats, Milani, D.;
Ruiz-Ruano, F.J.; Camacho, J.P.M.; Cabral-de-Mello, D.C., Heredity 127:
475–483, (view at publisher).
2021 Long-term persistence of supernumerary B chromosomes in multiple
species of Astyanax fish, Silva, D.M.Z.A.*; Ruiz-Ruano, F.J.*; Utsunomia,
R.; Martín-Peciña, M.; Castro, J.P.; Freire, P. P.; Carvalho, R.F.; Hashimoto,
D.T.; Suh, A.; Oliveira, C.; Porto-Foresti, F.; Artoni, R.F.; Foresti, F.; Camacho,
J.P.M., BMC Biology 19: 52, *equal contribution, (view at publisher).
2021 A long-term conserved satellite DNA that remains unexpanded in
several genomes of Characiformes fish is actively transcribed, Zeni dos
Santos, R.*; Milan Calegari, R.*; Silva, D. M. Z. A.; Ruiz-Ruano, F. J.; Melo,
S.; Oliveira, C.; Foresti, F.; Uliano-Silva, M.; Porto-Foresti, F.; Utsunomia, R.,
Genome Biology and Evolution 13(2): evab002, *equal contribution, (view
at publisher).
2021 Mind the numt: finding informative mitochondrial markers in a gi-
ant grasshopper genome, Pereira, R.J.*; Ruiz-Ruano, F.J.*; Thomas, C.;
Pérez-Ruiz, M.; Jiménez-Bartolomé, M.; Liu, S.; de La Torre, J.; Bella, J.,
Journal of Zoological Systematics and Evolutionary Research
59(3): 635–645., *equal contribution, (view at publisher).
2020 Development and characterization of 15 novel polymorphic microsatel-
lite loci for two important bot flies (Diptera, Oestridae) by next-
generation sequencing, Aleix-Mata, G.; Expósito, M.; Ruiz-Ruano, F.J.;
López-Beceiro, A.M.; Fidalgo, L.E.; Martínez-Carrasco, C.; Ruiz de Ybáñez,
M.R.; Boos, M.; Pérez, J.M.; Sánchez, A., Parasitology Research, 119:
2829-2835, (view at publisher).
2020 Satellitome analysis in the ladybird beetle Hippodamia variegata (Co-
leoptera, Coccinellidae), Mora, P.; Vela, J.; Ruiz-Ruano, F.J.; Ruiz-Mena,
A.; Montiel, E. E.; Palomeque, T.; Lorite, P., Genes 11(7): 783, (view at
publisher).
2020 High dynamism for neo-sex chromosomes: satellite DNAs reveal com-
plex evolution in a grasshopper, Ferretti, A.B.; Milani, D.; Palacios-Gimenez,
O.M.; Ruiz-Ruano, F.J.; Cabral-de-Mello, D.C., Heredity, 125: 124–137,
(view at publisher).
2020 Eight million years of satellite DNA evolution in grasshoppers of the
genus Schistocerca illuminate the ins and outs of the library hypo-
thesis, Palacios-Gimenez, O.M.; Milani, D.; Song, H.; Marti, D.A.; López-León,
M.D.; Ruiz-Ruano, F.J.; Camacho, J.P.M.; Cabral-de-Mello, D.C., Genome
Biology and Evolution, 12(3): 88–102, (view at publisher).
2020 The complete mitochondrial genome of Talpa aquitania (Talpidae;
Insectivora), a mole species endemic to northern Spain and southern
France, Aleix-Mata, G.; Gutiérrez, J.; Ruiz-Ruano, F.J.; Lorite, P.; Marchal,
J. A.; Sánchez, A, Molecular Biology Reports, 47(3): 2397–2403, (view at
publisher).
2020 Satellite DNA content of B chromosomes in the characid fish Chara-
cidium gomesi supports their origin from sex chromosomes, Serrano-
Freitas, E.A.*; Silva, D.M.Z.A.*; Ruiz-Ruano, F.J.; Utsunomia, R.; Araya-
Jaime, C.; Oliveira, C.; Camacho, J.P.M.; Foresti, F., Molecular Genetics
and Genomics, 295: 195–207, *equal contribution, (view at publisher).
2019 Programmed DNA elimination of germline development genes in song-
birds, Kinsella, C.M.*; Ruiz-Ruano, F.J.*; Dion-Côté, A.M.; Charles, A.J.;
Gossmann, T.I.; Cabrero, J.; Kappei, D.; Hemmings, N.; Simons, M.J.P.; Ca-
macho, J.P.M.; Forstmeier, W.; Suh, A., Nature Communications, 10: 5468,
*equal contribution, (view at publisher).
2019 Differential expression of genes related to sexual determination can
modify the reproductive cycle of Astyanax scabripinnis (Characi-
formes: Characidae) in B chromosome-carrier individuals, Castro, J.P.;
Hattori, R.S.; Yoshinaga, T.T.; Silva, D.M.Z.A.; Ruiz-Ruano, F.J.; Foresti, F.;
Santos, M.H.; Almeida, M.C.; Moreira-Filho, O.; Artoni, R.F., Genes, 10(11):
909, (view at publisher).
2019 DNA metabarcoding of Neotropical ichthyoplankton: Enabling high
accuracy with lower cost, Nobile, A.B.; Freitas-Souza, D.; Ruiz-Ruano,
F.J.; Nobile, M.L.M.O.; Costa, G.O.; de Lima, F.P.; Camacho, J.P.M.; Foresti,
F.; Oliveira, C., Metabarcoding and Metagenomics, 3: e35060, (view at
publisher).
2019 How dynamic could be the 45S rDNA cistron? An intriguing variab-
ility in a grasshopper species revealed by integration of chromosomal
and genomic data, Ferretti, A.B.S.M.*, Ruiz-Ruano, F.J.*; Milani, D.;
Loreto, V.; Martí, D.A.; Ramos. E.; Martins, C.; Cabral-de-Mello, D.C., Chro-
mosoma, 128(2): 165–175, *equal contribution, (view at publisher).
2019 Satellitome landscape analysis of Megaleporinus macrocephalus (Tele-
ostei, Anostomidae) reveals intense accumulation on the hetero-
morphic sex chromosome, Utsunomia, R.; Silva, D.M.Z.A.; Ruiz-Ruano,
F.J.; Góes, C.A.G.; Melo, S.; Peres-Ramos L.; Oliveira, C.; Porto-Foresti, F.;
Foresti, F.; Hashimoto, D.T., Scientific Reports, 9: 5856, (view at publisher).
2019 Complete mitochondrial genome of the Western Capercaillie Tetrao
urogallus (Phasianidae, Tetraoninae), Aleix-Mata, G.; Ruiz-Ruano, F.J.;
Pérez, J.M.; Sarasa, M.; Sánchez, A., Zootaxa, 4550(4): 585-593, (view at
publisher).
2019 Gene expression changes elicited by a parasitic B chromosome in the
grasshopper Eyprepocnemis plorans are consistent with its pheno-
typic effects, Navarro-Domínguez, B.; Martín-Peciña, M.; Ruiz-Ruano, F.J.;
Cabrero, J.; Corral, J.M.; López-León, M.D.; Sharbel, T.F.; Camacho, J.P.M.,
Chromosoma, 128(1): 53–67, (view at publisher).
2019 Full plastome sequence in the fern Vandenboschia speciosa (Hymen-
ophyllales): structural singularities and evolutionary insights, Ruiz-
Ruano, F.J.; Navarro-Domínguez, B.; Camacho, J.P.M.; Garrido-Ramos, M.A.,
Journal of Plant Research, 132(1): 3–17, (view at publisher).
2019 Characterization of the satellitome in lower vascular plants: the case
of the endangered fern Vandenboschia speciosa, Ruiz-Ruano, F.J.;
Navarro-Domínguez, B.; Camacho, J.P.M.; Garrido-Ramos, M.A., Annals of
Botany 123(4): 587–599, (view at publisher).
2019 Phylogenetic signal of genomic repeat abundances can be distorted
by random homoplasy: A case study from hominid primates, Martín-
Peciña, M.; Ruiz-Ruano, F.J.; Camacho, J.P.M.; Dodsworth, S., Zoological
Journal of the Linnean Society, 185(3): 543–554, (view at publisher).
2018 High throughput analysis of satellite DNA in the grasshopper Pyr-
gomorpha conica reveals abundance of homologous and heterologous
higher-order repeats, Ruiz-Ruano, F.J.; Castillo-Martínez, J.; Cabrero,
J.; Gómez, R.; Camacho, J.P.M.; López-León, M.D., Chromosoma 127(3):
323–340, (view at publisher).
2018 Quantitative sequence characterization for repetitive DNA content in
the supernumerary chromosome of the migratory locust, Ruiz-Ruano,
F.J.; Cabrero, J.; López-León, M.D.; Sánchez, A.; Camacho, J.P.M., Chromo-
soma 127(1): 45–57, (view at publisher).
2017 Transcription of a B chromosome CAP-G pseudogene does not in-
fluence normal Condensin Complex genes in a grasshopper, Navarro-
Domínguez, B; Ruiz-Ruano, F.J.; Camacho, J.P.M.; Cabrero, J.; López-León,
M.D., Scientific Reports 7: 17650, (view at publisher).
2017 High-throughput analysis unveils a highly shared satellite DNA lib-
rary among three species of fish genus Astyanax, Silva, D.M.Z.A.; Ut-
sunomia, R.; Ruiz-Ruano, F.J.; Daniel, S.N.; Porto-Foresti, F.; Hashimoto,
D.T.; Oliveira, C.; Camacho, J.P.M.; Foresti, F., Scientific Reports 7: 12726,
(view at publisher).
2017 First complete female mitochondrial genome in four bivalve species
genus Donax and their phylogenetic relationships within the Vener-
oida order, Fernández-Pérez, J.; Nantón, A.; Ruiz-Ruano, F.J.; Camacho,
J.P.M.; Méndez, J., PLoS ONE 12(9): e0184464, (view at publisher).
2017 A glimpse into the satellite DNA library in Characidae fish (Teleostei,
Characiformes), Utsunomia R.; Ruiz-Ruano, F.J.; Silva, D.M.Z.A; Serrano,
E.A.; Rosa, I.F.; Scudeler, P.E.S.; Hashimoto, D.T.; Oliveira, C.; Camacho,
J.P.M.; Foresti, F., Frontiers in Genetics 8: 103, (view at publisher).
2017 Protein-coding genes in B chromosomes of the grasshopper Eypre-
pocnemis plorans, Navarro-Domínguez, B.; Ruiz-Ruano, F.J.; Cabrero, J.;
Corral, J.M.; López-León, M.D.; Sharbel, T.F.; Camacho, J.P.M., Scientific
Reports 6: 45200, (view at publisher).
2017 Post-meiotic B chromosome expulsion, during spermiogenesis, in two
grasshopper species, Cabrero, J.; Martín-Peciña, M.; Ruiz-Ruano, F.J.;
Gómez, R.; Camacho, J.P.M., Chromosoma 126(5): 633–644, (view at pub-
lisher).
2017 Satellite DNA content illuminates the ancestry of a supernumerary
(B) chromosome, Ruiz-Ruano, F.J.; Cabrero, J.; López-León, M.D.; Ca-
macho, J.P.M., Chromosoma 126(4): 487–500, (view at publisher).
2016 The complete mitochondrial genome sequence of Astyanax paranae
(Teleostei: Characiformes), Silva, D.M.Z.A.; Utsunomia, R.; Ruiz-Ruano,
F.J.; Oliveira, C.; Foresti, F., Mitochondrial DNA Part B: Resources
1(1): 586–587, (view at publisher).
2016 High-throughput analysis of the satellitome illuminates satellite DNA
evolution, Ruiz-Ruano, F.J.; López-León, M.D.; Cabrero, J.; Camacho,
J.P.M., Scientific Reports 6: 28333, (view at publisher).
2016 Origin of B chromosomes in the genus Astyanax (Characiformes,
Characidae) and the limits of chromosome painting, Silva, D.M.Z.A.;
Daniel, S.N.; Camacho, J.P.M.; Utsunomia, R.; Ruiz-Ruano, F.J.; Penitente,
M.; Pansonato-Alves, J.C.; Hashimoto, D.T.; Oliveira, C.; Porto-Foresti, F.;
Foresti, F., Molecular Genetics and Genomics 291(3): 1407–1418, (view
at publisher).
2016 Uncovering the ancestry of B chromosomes in Moenkhausia sanc-
taefilomenae (Teleostei, Characidae), Utsunomia, R.; Silva, D.M.Z.A.;
Ruiz-Ruano, F.J.; Araya-Jayme, C.; Pansonato-Alves, J.C.; Hashimoto, D.T.;
Oliveira, C.; Trifonov, V.A.; Porto-Foresti, F.; Camacho, J.P.M.; Foresti, F.,
PLoS ONE 11(3): e0150573, (view at publisher).
2016 DNA barcoding of Iberian Peninsula and North Africa Tawny Owls
Strix aluco suggests the Strait of Gibraltar as an important barrier
for phylogeography, Doña, J.*; Ruiz-Ruano, F.J.*; Jovani, R., Mitochon-
drial DNA 26(6): 4475–4478, *equal contribution, (view at publisher).
2015 Transient microgeographic clines during a B chromosome invasion,
Camacho, J.P.M.; Shaw, M.W.; Cabrero, J.; Bakkali, M.; Ruiz-Estévez, M.; Ruiz-
Ruano, F.J.; Martín-Blázquez, R.; López-León M.D., American Naturalist
186(5): 675–681, (view at publisher).
2015 Intragenomic distribution of RTE retroelements suggests intra-
chromosomal movement, Montiel, E.E.*; Ruiz-Ruano, F.J.*; Cabrero,
J.; Marchal, J.A.; Sánchez, A.; Perfectti, F.; López-León, M.D.; Camacho,
J.P.M., Chromosome Research, 23(2): 211–223, *equal contribution, (view
at publisher).
2015 Non-random expression of rDNA units in a grasshopper showing high
intragenomic variation for the ITS2 region, Ruiz-Estévez, M.*; Ruiz-
Ruano, F.J.*; Cabrero, J.; Bakkali, M.; Perfectti, F.; López-León, M.D.;
Camacho, J.P.M., Insect Molecular Biology 24(3): 319–330, *equal contri-
bution, (view at publisher).
2015 Genomics of ecological adaptation in cactophilic Drosophila, Guillén,
Y.; Rius, N.; Delprat, A.; Williford, A.; Muyas, F.; Puig, M.; Casillas, S.; Ràmia
M.; Egea, R.; Negre, B.; Mir, G.; Camps, J.; Moncunill, V.; Ruiz-Ruano, F.J.;
Cabrero, J.; de Lima, L.G.; Dias, G.B.; Ruiz, J.C.; Kapusta, A.; Garcia-Mas, J.;
Gut, M.; Gut, I.G.; Torrents, D.; Camacho, J.P.M.; Kuhn, G.C.S.; Feschotte,
C.; Clark, A.G.; Betrán, E.; Barbadilla, A.; Ruiz, A., Genome Biology and
Evolution 7(1): 349–366, (view at publisher).
2015 A step to the gigantic genome of the desert locust: chromosome sizes
and repeated DNAs, Camacho, J.P.M.; Ruiz-Ruano, F.J.; Martín-Blázquez,
R.; López-León, M.D.; Cabrero, J.; Lorite, P.; Cabral-de-Mello, D.C.; Bakkali,
M., Chromosoma 124(2): 263-275, (view at publisher).
2015 Next generation sequencing and FISH reveal an uneven and non ran-
dom microsatellite distribution in grasshopper giant genome, Ruiz-
Ruano, F.J.; Cuadrado, A.; Montiel, E.E.; Camacho, J.P.M.; López-León,
M.D., Chromosoma 124(2): 221–234, (view at publisher).
2014 U1 snDNA clusters in grasshoppers: chromosomal dynamics and gen-
omic organization, Anjos, A.; Ruiz-Ruano, F.J.; Camacho, J.P.M.; Loreto,
V.; Cabrero, J.; de Souza, M.J.; Cabral-de-Mello, D., Heredity 114: 207–219,
(view at publisher).
2014 Disparate molecular evolution of two types of repetitive DNAs in the
genome of the grasshopper Eyprepocnemis plorans, Teruel, M.; Ruiz-
Ruano, F.J.; Marchal J.A.; Sánchez-Baca, A.; Cabrero, J.; Camacho, J.P.M.;
Perfectti, F., Heredity 112: 531–542, (view at publisher).
2014 Delimiting the origin of a B chromosome by FISH mapping, chro-
mosome painting and DNA sequence analysis in Astyanax paranae
(Teleostei, Characiformes), Silva, D.M.Z.A.; Pansonato-Alves, J.C.; Utsuno-
mia, R.; Araya-Jaime, C.; Ruiz-Ruano, F.J.; Daniel, S.N.; Hashimoto, D.T.;
Oliveira, C.; Camacho, J.P.M.; Porto-Foresti, F.; Foresti, F., PLoS ONE 9(4):
e94896, (view at publisher).
2014 Peripatric origin of the only cave-restricted plecopteran insect known,
Ruiz-Ruano, F.J.; Camacho, J.P.M; Cabrero, J.; López-Rodríguez, M.J.;
Tierno de Figueroa, J.M., Arthropod Systematics & Phylogeny 72(1):
3–10, (view at publisher).
2014 A mobile insulator system to detect and disrupt cis-regulatory land-
scapes in vertebrates, Bessa, J.; Luengo, M.; Rivero-Gil, S.; Ariza-Cosano,
A.; Maia, A.H.; Ruiz-Ruano, F.J.; Caballero, P.; Naranjo, S.; Carvajal, J.J.;
Gómez-Skarmeta, J.L., Genome Research 24(3): 487–495, (view at publisher).
2013 The Ku70 DNA-repair protein is involved in centromere function in
a grasshopper species, Cabrero, J.; Bakkali, M.; Navarro-Domínguez, B.;
Ruiz-Ruano, F.J.; Martín-Blázquez, R.; López-León, M.D.; Camacho, J.P.M.,
Chromosome Research 21: 393–406, (view at publisher).
2011 DNA amount of X and B chromosomes in the grasshoppers Eypre-
pocnemis plorans and Locusta migratoria, Ruiz-Ruano, F.J.; Ruiz-
Estévez, M.; Rodríguez-Pérez, J.; López-Pino J.L.; Cabrero J.; Camacho, J.P.M.,
Cytogenetic and Genome Research 134: 120–126, (view at publisher).
53 peer-reviewed publications (17 as first author + 6 as corresponding author +
2 senior author)
Years of publishing: 13
Total citations: 1287
h-index: 30
i10-index: 34
All citation metrics were obtained through Google Scholar (ht-
tps://scholar.google.es/citations?user=p9O0HK8AAAAJ, last update 13
January 2023).
 Papers published in: Chromosoma (8), Scientific Reports (5), Genome Biology
and Evolution (4), Heredity (4), Chromosome Research (3), PLoS ONE (3),
BMC Biology (2), Genes (2), Molecular Genetics and Genomics (2), Annals of
Botany (1), Arthropod Systematics & Phylogeny (1), Cytogenetic and Genome
Research (1), Frontiers in Genetics (1), Genome (1), Genome Research (1), Insect
Molecular Biology (1), Journal of Plant Research (1), Journal of Zoological
Systematics and Evolutionary Research (1), Metabarcoding and Metagenomics
(1), Mitochondrial DNA Part A (1), Mitochondrial DNA Part B (1), Molecular
Biology Reports (1), Nature Communications (1), Parasitology Research (1),
PNAS (1), The American Naturalist (1), Zoological Journal of the Linnean
Society (1) and Zootaxa (1).

Book chapters
2021 Satellite DNA is an inseparable fellow traveller of B chromosomes,
Camacho, J.P.M.; Ruiz-Ruano, F. J.; López-León, M.D.; Cabrero, J., In:
Satellite DNAs in Physiology and Evolution (Ed. Ugarković, Ð.),
pages 85–102, (view at publisher).
2015 Grasshoppers (Orthoptera), Camacho, J.P.M.; Cabrero, J.; López-León
M.D.; Cabral-de-Mello, D.C.; Ruiz-Ruano, F.J., In: Protocols for Cyto-
genetic Mapping of Arthropod Genomes (Ed. Sharakhov, I.V.), pages
381–438, (view at publisher).
Invited editor of a research topic
2022 Cytogenomics: Structural Organization and Evolution of Genomes,
Utsunomia, R.; Ruiz-Ruano, F. J.; Vaio, M. (editors), Frontiers in Genetics,
(view at publisher).

Selected press cover in English

Scientific American 01 November 2019: “Solving the Mystery of Songbird Di-
versity – A strange chromosome may have provided fodder for the evolution of
new traits”
Phys.org 28 October 2016: “Expanded genetic information about the migratory
locust”

Referee assignments
- Frontiers in Genetics and Frontiers in Ecology & Evolution: Review Editor in
the Specialty Evolutionary and Population Genetics
- Biology: Reviewer Board Member
- BMC Evolutionary Biology (2), BMC Genomics (5), Cell Reports (1), Frontiers
in Genetics (2) Genes (6), Genome Biology (2), Genome Biology and Evolution
(6), International Journal of Molecular Sciences (1), Insects (1), Molecular Ecology
Resources (2), Nature Protocols (2), Peer Community In Genomics (1), PLoS
ONE (1) and Scientific Reports (3)
- Certified Publons Academy Peer Reviewer (Publons profile)
- Project reviewer: ETH Zurich (Switzerland), Czech Science Foundation (GACR)
 International network memberships
2020 - present Germline-Restricted Chromosomes Consortium.

Professional services
2013 Programming and implementation of a template in LaTeX code (trans-
lated), University of Granada, 100 hours.
2011 I Lecture series Does science test?, University of Granada, 18 hours,
Organization.

Invited talks
2022 Talk: A chromosome to rule them all songbirds, Unit of Evolutionary
Biology/Systematic Zoology, University of Potsdam (Germany), 1 hour.
2021 Talk: Knowing the genomic content of additional chromosomes, Insti-
tute of Cytology and Genetics, Russian Academy of Sciences, Siberian Department
(Russia), 1 hour.
2021 Talk: Satellite DNA: a toolbox for cytogenomics, Institute of Cytology
and Genetics, Russian Academy of Sciences, Siberian Department (Russia), 1
hour.
2020 Talk: Germline restricted chromosomes: what we know and what is
coming, Seminars of the Area of Cytogenetics and Evolution. Instituto de
Botánica del Nordeste, Corrientes (Argentina), 2 hours.
2018 Talks about Evolutionary Biology, Universidad Peruana Cayetano Heredia
(Peru), 2 hours.
2013 I Lecture Series of Thesis Projects, Department of Genetics, University of
Granada, 2 hours.
2013 IV Lecture Series of Young Researchers, University of Granada, 1 hour.

Oral presentations in conferences

2022 A GRC contig in a haystack, I GRC Workshop, Prague (Czech Republic),
20 - 21 August.
2022 The complex story of an old germline-restricted chromosome, ESEB
2022, Prague (Czech Republic), 14 - 19 August.
2020 A glimpse into the genomic structure of the zebra finch germline-
restricted chromosome, IV Uppsala Transposon Symposium, Uppsala
(Sweden), 30 September - 2 October.
2020 Evolution and function of the zebra finch germline-restricted chromo-
some, SMBE2020: Virtual symposium on Within-individual genome variation
and germline / soma distinction, 29 June.
2019 Diverse evolutionary trajectories in germline-restricted chromosomes
of songbirds, III Uppsala Transposon Symposium, Uppsala (Sweden), 27
September.
2019 Programmed DNA elimination of germline development genes in song-
birds, Symposium on The Evolution of Cooperation and Conflict: from theory
to experimental evaluation, Uppsala (Sweden), 21 - 22 May.
2017 Searching for the geographical origin of a B chromosome in the african
grasshopper Eyprepocnemis plorans (translated title), I Symposium of
Evolution, Genetics and Taxonomy, Lima (Peru), 18 - 19 December.
2016 The private life of the satellite DNA (translated title), XVI Latinoamer-
ican Conference of Genetics (ALAG), Montevideo (Uruguay), 9 - 12 October.
2016 I am a miner (of DNA satellite) (translated title), IX Seminar of Cyto-
genetics of the Spanish Society of Genetics, Toledo (Spain), 8 - 10 June.
2014 Content of repetitive DNA of the B chromosomes of the grasshopper
Eyprepocnemis plorans (translated title), XX Seminar of Population Ge-
netics and Evolution of the Spanish Society of Genetics, Granada (Spain), 1 - 3
October.
2014 Searching for the geographical origin of a B chromosome (translated
title), VIII Seminar of Population Genetics and Evolution of the Spanish Society
of Genetics, Granada (Spain), 1 - 3 October.
2014 The genomics to the rescue of the cytogenetics (translated title), VIII
Seminar of Cytogenetics of the Spanish Society of Genetics, Alcalá de Henares
(Spain), 24 - 26 June.
2014 Searching for the geographical origin of a B chromosome, III B-
Chromosome Conference, Gatersleben (Germany), 7 - 9 April.
2011 Dis-concerted evolution of rDNA 45S Internal Transcribed Spacers in
the grasshopper Locusta migratoria (translated title), XXXVIII Con-
ference of the Spanish Society of Genetics, Murcia, 21 - 23 September.
2010 How much DNA has a B chromosome? (translated title), VI Seminar
of Cytogenetics of the Spanish Society of Genetics, Córdoba, 29 September - 2
October.

Posters in conferences and workshops

2022 Towards a zebra finch pangenome, 55 Population Genetics Group, Norwich
(United Kingdom), 5 - 7 January.
2014 Genomic search for satellite DNA contained in the B chromosomes of
Locusta migratoria, III B-Chromosome Conference, Gatersleben (Germany),
7 - 9 April.
2011 Dis-concerted evolution of rDNA 45S Internal Transcribed Spacers in
the grasshopper Locusta migratoria (translated title), III Meeting of
the Spanish Society for Evolutionary Biology, Madrid (Spain), 21 - 25 November.

Specialization courses
2022 Single cell RNAseq data analysis, National Bioinformatics Infrastructure
of Sweden (NBIS), 45 hours.
2021 K-mer workshop: "Oh-know", Research School in Biosystematics, University
of Oslo, 20 hours.
2021 Quick and clean: advanced Python for data science in biology, National
Bioinformatics Infrastructure of Sweden (NBIS), 40 hours.
2021 Genome Assembly using Oxford Nanopore Sequencing, Physalia-courses,
30 hours.
 2017 Workshop in Teaching Bioinformatics, University of Granada.
2015-2016 Applied Academic English (1st edition), University of Granada, 40 hours.
2013 Computing for Data Analysis (in R language), Johns Hopkins Bloomberg
School of Public Health (Coursera.org), 4 weeks.
2012 Writing in the Science, University of Stanford (Coursera.org), 8 weeks.
2012 X Course of Phylogenies and DNA Genealogies: Resconstruction and
Applications, University of Barcelona, 70 hours.
2012 I Course of Advanced Programming in Python, E-learning Centre, Uni-
versity of Granada, 30 hours.
2012 Workshop in Ximdex, Open Ximdex Evolution SL, 4 hours.
2011 I Lecture series Does science test?, University of Granada, 18 hours.
2010 Blogs and Science, University of Granada, 10 hours.
2009 Legal Issues of Open Source, CENATIC, 3 hours.
2009 Darwin and the Theory of the Evolution by Natural Selection, Univer-
sity of Granada, 22 hours.
2009 Career Opportunities in Biology, University of Granada, 10 hours.
2009 I Conference of Bioinformatics in Granada, University of Granada, 5
hours.
2009 Biology: Beyond the Degree, University of Granada, 8 hours.
2009 I Workshop in The Free Software Applications in Biomedicine, Univer-
sity of Granada, 5 hours.

Teaching and Supervisory Experience

Postgraduate courses
2022 Instructor in Worshop on Genome annotation, Datascience and Bioin-
formatics module, MRes Evolution and Genomics, University of East Anglia,
6 hours.
2019 Next Generation Sequencing: principles and applications, Postgradu-
ation Course, São Paulo State University (UNESP), Rio Claro (Brazil), 30
hours.
2019 Satellite DNA Analysis, Postgraduation Course, Federal University of Pern-
ambuco, Recife (Brazil), 3 hours.
2018-2019 Genetic Analysis Techniques, Master of Genetics and Evolution, University
of Granada, 8 hours.
2017-2018 Genetic Analysis Techniques, Master of Genetics and Evolution, University
of Granada, 8 hours.
2016 Introduction to Next Generation Sequencing Analysis, Postgraduation
Course, São Paulo State University (UNESP), Botucatu (Brazil), 30 hours.

Graduate courses
2012 III Lecture Series of Young Researchers, University of Granada, 1 hour.
 2011 II Course of Basic Techniques in Environmental Biology Research,
University of Granada, 2 hours.
2009 Biology: Beyond the Degree, University of Granada, 10 hours.
2009 I Workshop The Free Software Applications in Biomedicine, University
of Granada, 1 hour.
2009-2011 Workshops about Free Software (LYX and LATEX-Beamer), University
of Granada, 20 hours.

Undergraduate courses
2019 Interdisciplinary projects 3, Universidad de Ingeniería y Tecnología - UTEC
(Peru), 28 hours.
2018-2019 Practicum in Genetics I, Biology Degree, University of Granada, 25.5 hours.
2018-2019 Practicum in Human Genetics, Biology Degree, University of Granada, 19
hours.
2017-2018 Practicum in Genetics I, Biology Degree, University of Granada, 24.5 hours.
2016-2017 Practicum in Genetics, Biotechnology Degree, University of Granada, 9 hours.
2016-2017 Practicum in Evolutionary Biology, Biology Degree, University of Granada,
16 hours.
2016-2017 Practicum in Genetics II, Biology Degree, University of Granada, 15 hours.
2015-2016 Practicum in Genetics I, Biology Degree, University of Granada, 46 hours.
2015-2016 Practicum in Human Genetics, Biology Degree, University of Granada, 14
hours.
2014-2015 Practicum in Genetics II, Biology Degree, University of Granada, 40 hours.
2014-2015 Practicum in Genetics I, Biology Degree, University of Granada, 20 hours.
2013-2014 Practicum in Molecular Evolution, Biochemistry Degree, University of
Granada, 40 hours.
2013-2014 Practicum in Genetics I, Biology Degree, University of Granada, 20 hours.
2012-2013 Practicum in Genetics II, Biology Degree, University of Granada, 40 hours.

Co-supervision of PhD students

2022-2026 PhD Thesis: “Evolution of satellite DNA in vertebrate genomes”
(Inês Borges), University of Bonn, Main supervisor: Alexander Suh.
2021-2025 PhD Thesis: “Expression of the zebra finch germline-restricted chro-
mosome” (Niki Vontzou), University of East Anglia, Main supervisor: Alex-
ander Suh.

Supervision of Master students

2017-2018 Master Thesis: The satellitome of Drosophila genus (Álvaro Sánchez
Villalba), Master of Genetics and Evolution, University of Granada.
2016-2017 Master Thesis: Leafing through the catalog of the library satellite
DNA in the genus Drosophila (Alba María Talavera Rodríguez), Mas-
ter of Genetics and Evolution, University of Granada.
 Co-supervision of Master students
2021-2022 Master Thesis: Characterization of the evolution of satellite DNA
across Passeriformes (Inês Borges), Master in Bioinformatics, Uppsala
University, Main supervisor: Alexander Suh.
2020-2021 Master Thesis: Investigation of the parasitic origin of the germline-
restricted chromosome (GRC) of Ficedula albicollis by transcriptomic
analysis (Carmen Hernández Ruiz), Master of Genetics and Evolution,
University of Granada, Main supervisor: Juan Pedro M. Camacho.
2020-2021 Research Training (15 credits): Characterization and evolution of
satDNA in songbird genomes (Inês Borges), Master in Bioinformatics,
Uppsala University, Main supervisor: Alexander Suh.
2019-2020 Master Thesis: Development and validation of bioinformatic meth-
ods for GRC assembly and annotation (Roberto Rossini), Master in
Bioinformatics, Uppsala University, Main supervisor: Alexander Suh.
2017-2018 Master Thesis: Transcriptomic analysis of wing development in a
grasshopper (Alberto Bellido Chaves), Master of Genetics and Evolution,
University of Granada, Main supervisor: Juan Pedro M. Camacho.

Thesis examination boards

2022 PhD thesis: “Genetic basis of idiopathic male infertility” by Miriam
Cerván Martín, University of Granada (Spain), External evaluator.
2022 Master thesis: “Um novo conjunto de DNAs satélites compartilhado
por todos os Characoidei (Characiformes, Teleostei)” [Translated title:
“A new collection of satellite DNAs shared by all Characoidei (Chara-
ciformes, Teleostei)”] by Leonardo Moura Giglio, São Paulo State Uni-
versity (UNESP), Bauru (Brazil), Panel member.
2021 PhD thesis: “Mecanismos de regresión y activación testicular en
mamíferos con reproducción estacional: control genético y suscept-
ibilidad a cambios ambientales” [Translated title: “Mechanisms of
testicular regression and activation in mammals with seasonal repro-
duction: genetic control and susceptibility to environmental changes”]
by Miguel Lao Pérez, University of Granada (Spain), External evaluator.
2021 Master thesis: “Caracterização e análises citogenômicas de satelito-
mas de Serrasalmideos (Teleostei, Characiformes)” [Translated title:
“Characterization and cytogenomic analyses of the satellitomes in
Serrasalmideos (Teleostei, Characiformes)”] by Pedro Henrique de
Mira Rodrigues, São Paulo State University (UNESP), Bauru (Brazil), Panel
member.
2020 PhD thesis: “Análisis estructural y funcional del cromosoma B de
centeno” [Translated title: “Structural and functional analysis of the
rye B chromosome”] by Fernando Gómez-Aldecoa Cuadrado, Com-
plutense University of Madrid (Spain), External evaluator.

Pedagogical and supervisory training

2021 Curious about Leadership Course, Uppsala University, 2 days.
2020 Supervisor Training Course, Uppsala University, 2 days.
 2020 Postdoc Training Course, Uppsala University, 15 days (biweekly during 7
months).
2014-2015 VII Introductory Course to the Academic Teaching, University of
Granada, 200 hours.
2014-2015 VI Call of Support for Beginner Teaching Training and Improvement
of Teaching, University of Granada, 40 hours.
2014 Academic English (2nd edition), University of Granada, 20 hours.
2013-2014 V Call of Support for Beginner Teaching Training and Improvement
of Teaching, University of Granada, 40 hours.
2011-2013 Teacher Education Program for PhD students, Department of Genetics,
University of Granada.

Outreach
2018 XVIII Week of the Science, University of Granada, 20 hours.
2017 XX Science Fair, Science Park of Granada, 8 hours.
2017 XVII Week of the Science, University of Granada, 20 hours.
2015 XVIII Science Fair, Science Park of Granada, 8 hours.
2015 XV Week of the Science, University of Granada, 20 hours.
2014 XIV Week of the Science, University of Granada, 20 hours.
2013 Talk in “Desgranando Ciencia”, Science Park of Granada, 1 hours.
2013 XVI Science Fair, Science Park of Granada, 8 hours.
2013 XIII Week of the Science, University of Granada, 20 hours.
2012 XV Science Fair, Science Park of Granada, 8 hours.
2012 XII Week of the Science, University of Granada, 20 hours.
2008 Talk “Evolution: The life’s reason”, Council of Priego de Córdoba (Cór-
doba) and I.E.S. Iliberis of Atarfe (Granada), 4 hours.

Blogs on science communication

2014 - present Little Bioinformatician, Divulgation about free software and bioinformatics,
(link).
2008 - present Un Bioinformatiquillo, Divulgation about free software and bioinformatics,
(link).
2008 - 2011 EvoVagariO, Divulgation about Evolution, (link).

References
2019 - present Alexander Suh, Postdoc supervisor, University of East Anglia (United King-
dom), (contact).
2015 - present Diogo C. Cabral de Mello, Collaborator, UNESP - São Paulo State University
(Brazil), (contact).
2010 - present Juan Pedro M. Camacho, PhD and Postdoc supervisor, collaborator, Uni-
versity of Granada (Spain), (contact).