Q.Amplification of DNA is always between two primers. Why?

Ans:
For the amplification of specific sequence of a DNA ,we use two primers,Forward and reverse
primers.Both primers are designed so that primer anneal with one strand will have its 3 end
towards the centre of sequence which we want to amplify.By this newly made strand will be
made in that direction.Both primers are annealed at different strands i.e. one primer on sense
strand and other on antisense strand.

After the annealing of primers (as shown in figure above) Taq polymerase synthesizes
complementary DNA strands by incorporating deoxy ribonucleotides at the 3 end of each
primer.After first cycle of PCR, complementary strands of both DNA strand s are formed as
shown in figure below:

After first cycle, newly formed strands 1A,1B will be formed.Now second cycle will occur and
after second sycle we will have four new DNA strands and total 8 strands as shown below:

At this stage,All strands have our specific DNA sequence but none of them has exact ends of
specific region.But after the third cycle this is accomplished and we have two DNA molecules of
exact sequence we want,as shown in fig below:

After each cycle,number of molecules of specific sequence increases and this will be dominant
type.
Now, we can conclude that Specific sequence is amplified by using forward and reverse
primers on different strands in the way that their 3 end should be towards our desired
sequence.