General Biology

Osmosis Lab
Soontornworasate Nataphan, Sand
Mailueang Nathanicha, Ning
Srihakorth Nakin, Mammoth
Lohaburananont Gawin, Win
Thanyarat Lohapark, Ploy

1001

6/3/2017

Purpose
The purpose of this experiment is to demonstrate how the osmosis work.
Hypothesis
If the jelly and potato soak in distilled water, then the jelly and potato will swell.
If the jelly and potato soak in salt water, then the jelly and potato will shrink.

Material

6 Beakers (3 for jelly, 3 for potato)

Electronic Balance
Distilled water
Concentrated salt water
2 Jellies
2 Potato Pieces
Calculator
Knife
Weighing paper
Markers
Gloves
Lab Coat
Goggles

Method

1. Collect the materials that your group needs (3 Jelly and Half a Potato)
2. Use the knife to cut the potato into 3 equal pieces
3. Use the electronic balance to find the mass of your jelly. Remember to lay down a
piece of weighing paper and set to zero.
 4. Record your data
5. Record descriptive observations about the jelly prior to the experiment.
6. Measure the initial volume of the jelly and the potato and record your data.
7. Fill your 1st beaker halfway with distilled water. Put one of your jelly into the
beaker. Set the beaker aside for one hour. Label the beaker using marker.
8. Fill your 2st beaker with concentrated salt solution halfway. Put your 2nd jelly into
that beaker. Set beaker aside for one hour. Label the beaker using marker.
9. Put your 3rd jelly into an empty beaker without putting anything in it. That should
be your control.
10. Put the 4th beaker halfway with distilled water. Put one of your potato pieces into
the beaker. Set the beaker aside for one hour. Label the beaker.
11. Put the 5th beaker halfway with concentrated salt solution halfway. Put your
second potato pieces into that beaker and set it aside for an hour. Label the beaker.
12. Place your last piece of potato pieces into the last beaker, empty. That is your
control for the potato. Label the beaker.
13. Make sure you label all the beakers distilled water or concentrated salt
solution with some markers.
14. After one hour, come back and look at each of the beakers and record the data
volume and mass of both the jellies in two beaker and both the potato in two other
beakers.
15. Also record the descriptive data for what the jelly and the potato look like now
after being soaker for an hour.
16. Calculate the percent change in the size of each jelly and potato
a) Percent change in height (Height after- Height before/ Height before x
100% = ?)
b) Percent change in width
c) Percent change in length
d) Percent change in volume
e) Percent change in mass

Data tables

Data table A Volume and mass of jelly before and after soaking in soaking
and distilled water

Jelly 1 Jelly 2
Dimensions Initial: Before Final: After Initial: Before Final: After
Soaking Soaking in Soaking soaking in
Distilled Water Salt Water
Volume 4ml 5ml 4ml 4ml
Mass 5.06g 6.09g 5.12g 4.82g

Data table B Volume and mass of potato before and after soaking in soaking
and distilled water

Potato 1 Potato 2
Dimensions Initial: Before Final: After Initial: Before Final: After
Soaking Soaking in Soaking soaking in
Distilled Water Salt Water

Volume 3 3.4 3 2.5

Mass 3.18 3.51 2.9 2.56

Data table C Descriptive observations of the jelly before and after soaking in
distilled water and salt water

Jelly 1 Jelly 2
Descriptive Observations Before Descriptive Observations Before
Soaking in Distilled Water: Soaking in Salt Water:
the jelly was sticky the jelly was sticky
Descriptive Observations After Soaking
in Distilled Water:
the jelly was bigger, softer, and its color
was lighter
Descriptive Observations After Soaking
in Salt Water:
the jelly was smaller and softer
the salt water change the color into pale
pink

Data table D Descriptive observations of the potato before and after soaking in
distilled water and salt water

Potato 1
Descriptive Observations Before Soaking
in Distilled Water:
the potato was a little bit moist and it was
not too hard and too soft
Descriptive Observations After Soaking in
Distilled Water:
the potato was bigger and it look like it
has more water inside of it
Potato 2
Descriptive Observations Before
Soaking in Salt Water:
a little bit moist and was not too
hardand soft
Descriptive Observations After Soaking
in Salt Water:
the potato look shrinker and the size
was not change obviously

Bar graph

Jelly 1: Soaked in Distilled Jelly 2: Soaked in Salt Water

Water
Percent Change in Volume 25% 0.00%
Percent Change in Mass 16.91% -6.22%

Potato 1: Soaked in Potato 2 : Soaked in Salt

 Distilled Water Water

Percent Change in 13.33% -16.67%

Volume
Percent Change in Mass 9.40% -13.28%

Analyze Results

1. What happened to the jellies after being soaked in distilled water and salt water for
an hour? Why? Be descriptive and detailed in your answer. Use your knowledge of
membrane transport.

answer The jelly that being soaked in distilled water are bigger than the other one
that being soaked in salt water and it also swell. Not only the size but also the mass,
volume, and even the color of the solution in two beakers were different too which the
beaker that contains water has no color while the beaker that contains salt water has
pale pink color. This caused by the osmosis which is the process that water move in
and out of the membrane. The jelly that being soaked in salt water was smaller in
size, shrink, and the mass and volume was lower too because the salt water has
more concentration than the jelly so the water would move out more than move in the
 jelly and this why the solution has pale pink color so we can know that the jelly is
hypotonic and the salt water is hypertonic. For the jelly that being soaked in the
water, it was bigger in both of size and mass or volume because it has more
concentration than the water so the water move into the jelly more than move out and
this why it was bigger so the jelly would be hypertonic and the water would be
hypotonic. As you can see from the data table, we measured the mass and volume
and the results are the jelly that soaked in distilled water

1. What happened to the potato after being soaked in distilled water and salt water for
an hour? Be descriptive and detailed in your answer. Use your knowledge of
membrane transport.

answer From the experiment, the potato that being soaked in distilled water were
swell because according to the osmosis process, the water would move from the
solution that has less concentrate or hypotonic solution which in this state is water to
the solution that has more concentrate or hypertonic solution which is potato so the
water would move in more than move out of the potato. According to the data table,
the potatos mass and volume was change too. For the potato that soaked in salt
water, its volume and mass was decreased because in this state the potato is refer
to hypotonic solution and the salt water around is refer to hypertonic solution so
water would move out more than move in the potato.

Conclusion
Osmosis is the movement of water(solvent) through semi permeable membrane from
hypertonic to hypotonic. When jelly and potato were putted in distilled water, water will move
in and get bigger because inside them has higher concentrated solution. Conversely, when
both of them were putted in salt water, water will move out and get smaller because outside
them has higher concentrated solution.

Work Log

Name Job / Role Date Assignment complete or not

Nataphan Soontornworasate Record data and analyze 1/3/2017 Assignment complete

result questions,
hypothesis

Nakin Srihakorth Measure Volume , 1/3/2017 Assignment complete

method, material
Nathanicha Mailueang Fill water in beaker, 1/3/2017 Assignment complete
make data tables,
conclusion, purpose
Gawin Lohaburananont Record data, bar graph, 1/3/2017 Assignment complete
data tables
Thanyarat Lohapark Record data, measure 1/3/2017 Assignment complete
mass

Reference

McIntosh, N. (2014, November 6). Osmosis. Retrieved from

http://www.bbc.co.uk/schools/gcsebitesize/science/add_aqa_pre_2011/cells/cells4.shtml

Diffusion and passive transport (n.d.). from

https://www.khanacademy.org/science/biology/membranes-and-transport/passive-
transport/a/diffusion-and-passive-transport