Professional Documents
Culture Documents
SAFETY
o Laboratory Classification
o Routes of infection
Lungs
Mouth
Through the skin
Eyes
o Sterilization/disinfection/sanitation
Red heat (flaming)
Dry heat (hot air)
Steam under pressure (autoclaving)
Steam not under pressure (Tyndallization)
Filtration
ENUMERATION OF MICROORGANISMS
o Demo of filtration
ISOLATION OF ORGANISMS
o Gram stain
o Microscope demo
Gram Stain
1. Stain with crystal violet solution for 1 min.
2. Wash with tap water
3. Stain with iodine solution for 1 min.
4. Decolourise with 95% ethanol until no more stain comes away
5. Wash with tap water
6. Counterstain with safranin for 10 secs