Data pengamatan dan perhitungan

A. Data Pengamatan
Tabel 1. Nilai Absorbansi Kurva Baku Kloramfenikol
denganVariasi Konsentrasi

Konsentrasi Absorbansi
Rata-rata
(ppm) I II III
2 0.1606 0.15944 0.1593 0.1598
4 0.2452 0.2431 0.2449 0.2444
6 0.2585 0.2603 0.2600 0.2596
8 0.3457 0.3456 0.3444 0.3451
10 0.7200 0.7164 0.7127 0.7164

Tabel 2. Absorbansi Sampel Kloramfenikol ( = nm)

Waktu Absorbansi
Rata-rata
(menit) I II III
5 0.2810 0.2804 0.2794 0.2802
10 0.3476 0.3458 0.3476 0.3467
20 0.5801 0.5786 0.5772 0.5786
30 0.8820 0.8861 0.8851 0.8844
45 1.2802 1.2934 1.2805 1.2847
60 1.6047 1.6053 1.6140 1.608

KURVA BAKU KLORAMFENIKOL

Y = 0.10895X +
0.0287

r = 0.9712
a) 5 menit

0.2802 = 0.10895x + 0.0287

0.28020.0287
x=
0..10895

= 2.3084

Co = 2.3084 g/ml x 900ml

= 20,7756mg

20.7756 mg
Kadar terdisolusi =
x 100
300 mg

= 6.9252%

4
FK =
x 20.7756
900

= 0.0923mg

b) 10 menit

0.3467 = 0.10895x + 0.0287

0.34670.0287
x=
0..10895

= 2.9188

Co = 2.9188 g/ml x 900ml

= 26.2692mg

26.2692 mg
Kadar terdisolusi =
x 100
300 mg

= 8.7564%
 4
FK =
x 26.2692
900

= 0.1168mg

c) 20 menit

0.5786 = 0.10895x + 0.0287

0.57860.0287
x=
0..10895

= 5.0473

Co = 5.0473 g/ml x 900ml

= 45.4257mg

45.4257 mg
Kadar terdisolusi =
x 100
300 mg

= 15.1419%

4
FK =
x 45.4257
900

= 0.2019mg

d) 30 menit

0.8844 = 0.10895x + 0.0287

0.88440.0287
x=
0. .10895

= 7.8541
Co = 7.8541 g/ml x 900ml

= 70.6869mg

70.6869 mg
Kadar terdisolusi =
x 100
300 mg

= 23.5623%

4
FK =
x 7 0.6869
900

= 0.3142mg

e) 45 menit

1.2847 = 0.10895x + 0.0287

1.28470.0287
x=
0..10895

= 11.5282

Co = 11.5282 g/ml x 900ml

= 103.7538mg

103.7538 mg
Kadar terdisolusi =
x 100
300 mg

= 34.5846%

4
FK =
x 103.7538
900

= 0.4611mg
f) 60 menit

1.608 = 0.10895x + 0.0287

1.6080.0287
x=
0..10895

= 14.4956

Co = 14.4956 g/ml x 900ml

= 130.7538mg

130.4604 mg
Kadar terdisolusi =
x 100
300mg

= 43.4868%

4
FK =
x 130.4604
900

= 0.5798mg