MOUSE DOSAGE CALCULATOR

for IACUC/LARC Preferred Anesthetics/Analgesics

Notes:
1) Follow step-by-step instructions below to calculate injection dosage
2) Label all solutions with Working Concentration (from this calculator) and expiration date (30 days from preparation date)
3) Calculator is write-protected, direct questions to <<daniel.ferguson@ucsf.edu>>

STEP 1) Select Drug From Drop-Down Menu: STEP 5) Prepare Solution:

ADD:
>> Ketamine (100 mg/mL) Xylazine (20 mg/mL) Acepromazine (10 mg/mL) 0.50 volume Ketamine (mL)
(note: make sure your stock solution matches the concentration listed) 0.25 volume Xylazine (mL)
0.15 volume Acepromazine (mL)
TO:
STEP 2) Enter Average Mouse Weight (g): 4.11 dilutant volume (mL)

>> 30 TO GET
(note: the average adult B6 mouse weighs 25g) Working Concentrations:
9.90 [Ketamine] (mg/mL)
0.99 [Xylazine] (mg/mL)
STEP 3) Enter Number of Animals to Inject: 0.30 [Acepromazine] (mg/mL)

>> 15
(note: used to calculate preparation amount, increase or decrease as advised in Steps 5 & 6) STEP 6) Administer Injection Volume (mL) to Mouse:

0.30 ---> Mouse ---> ^ ^

STEP 4) Enter Desired Dose Amount (mg/kg): (- -)
Suggested: Via Route: ( v v )~~~~~
>> 70 Ketamine 70-100 mg/kg IP note: mouse not to scale
10 Xylazine 10-20 mg/kg
2 Acepromazine 2-3 mg/kg
 Drug Name Purpose Route [Stock] (mg/mL)
Meloxicam NSAID PO/IM/SC 5

Carprofen NSAID SC 50

Ketoprofen NSAID SC 100

Atipamezole Reversal Agent SC/IP 5

Buprenorphine Analgesic SC/IP 0.3

Ketamine Anesthetic IP 100

Xylazine Sedative/Mild Analgesic (Reversed with IP 20

Atipamezole (5 mg/mL)

Acepromazine Sedative IP 10

Ketamine Cocktail 100

IP
Xylazine 20
Ketamine 100
Xylazine Cocktail IP 20
Acepromazine 10

Notes:
1) This table forms the basis for values in "Calculator" tab.
2) Columns F through K (colored gray) contain formulae output values, change with caution.
3) Worksheet is protected to prevent unwanted alterations to formatting.
4) Direct questions to <<daniel.ferguson@ucsf.edu>>

Meloxicam (5 mg/mL)
Carprofen (50 mg/mL)
Ketoprofen (100 mg/mL)
Atipamezole (5 mg/mL)
Buprenorphine (0.3 mg/mL)
Ketamine (100 mg/mL)
Xylazine (20 mg/mL)
Acepromazine (10 mg/mL)
Ketamine (100 mg/mL) Xylazine (20 mg/mL)
Ketamine (100 mg/mL) Xylazine (20 mg/mL)
Acepromazine (10 mg/mL)
Dose (mg/kg) Total Prep (mL) X factor Stock (mL) Dilutant (mL) [Working] (mg/mL)
5 5 16.5 0.50 4.51 0.50

5 5 16.5 Increase # of Animal N/A 0.50

5 5 16.5 Increase # of Animal N/A 0.50

1 5 16.5 0.10 4.90 0.10

0.1 5 16.5 0.17 4.84 0.01

100 5 16.5 0.50 4.51 9.90

10 5 16.5 0.25 4.75 0.99

3 5 16.5 0.15 4.85 0.30

100 0.50 9.90

5 16.5 4.2575
10 0.25 0.99
100 0.50 9.90
10 5 16.5 0.25 4.109 0.99
3 0.15 0.30

3
Give (mL/mouse)
0.30

0.30

0.30

0.30

0.30

0.30

0.30

0.30

0.30

0.30