Main Parameters

Pressure
Temperature

Time
Ti

1
What is sterilization?
Sterilization is the process where the goal is to reach a sterile condition of the instruments
The keyword is sterile
EN556
EN 556 gives a definition of sterile
sterile:: Condition of a medical device that is free from viable micro
organisms
The scientific expression for free from micro organisms is defined as well in EN EN556
556
th theoretical
the th ti l probability
b bilit off th
there bbeing
i a viable
i bl micro
i organism
i presentt on ththe medical
di l ddevice
i shall
h ll bbe
equal or less than one in a million.
million.

Why Sterilization?
To kill micro organisms
To prevent cross-
cross-infection
To protect personnel and patient

2
History of micro organisms
1670: Van Leeuwenhoek
Leeuwenhoek discovers great numbers of bacteria's
bacteria s
1862: Pasteur proves that:
Bacteria's multiply by dividing
Infection is air
born
Boiling kills the bacteria
1870: Bastian concludes that 100 C is not killing all the bacteria
1880: Chamberland manufactures the first autoclave to boil on more than 100 C

Various Micro Organisms

Animal-like : Malaria and Hepatitis
Vegetable-like:
Mould (10 times the size of a bacteria)
Bacteria (10 times the size of a virus)
Bacillus or spores (high resistant bacteria)
Viruses: Needs living
material to multiply

3
Multiplication of Micro Organisms

0-7 C: Relatively no multiplication

10-50 C: Ideal for the multiplication

62-100 C: Most bacteria's die off

100-120 C: Spores die off at WET HEAT

4
 Dry Heat:
Heat Steam
Steam:
Oxidation of the Coagulation of the
micro organism micro organism

How to sterilize

Plasma-Low
Plasma-
Temperature: Ethylene Oxide:
Chemical reaction C
Chemical reaction

5
Saturated Steam
Pressure Temperature Relation:
- 1.0 bar - 120 C
- 1.1
1 1 bar - 121 C

- 2.0 bar - 134 C

- 2.2 bar - 135 C

Minimum Holdingg Time

re Time relation
- 134 to 138 C - 3 Minutes
- 121 to 124 C - 15 Minutes
- 105 to 108 C - 2 Hours

6
Air & Steam
Where theres air, theres no steam
Where theres no steam, theres no sterilization
Air obstructs the temperature from rising

Air Removal
Since air is causing a problem, the air
removal is an important part of the
sterilization process
Two removal principles:
Gravity autoclaves
Pre-vacuum autoclaves

7
Gravity Principle
Steam production in vessel
Steam rises and pushes up the air
Air escapes through a valve at the top of the vessel
Sterilization conditions are reached
Temperature and pressure are maintained for a fixed period of time
In ggravityy autoclaves 5-10% of air left

8
Pre-Vacuum Principle
Air removal through vacuum pump
Steam production or steam injection in vessel
Sterilization conditions are reached
Temperature and pressure are maintained for a fixed period of time
In pre-vacuum autoclaves 1% of air left

9
Piping Diagram

10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
Control System Cat 2007

29
The Main Board
y , which controls and monitors the pphysical
Contains the electronic microcontroller system, y pparameters of the pprocess
and performs the operation sequence of the machine according to the selected program.
The dimensions of the main board are 30x15x10cm.
The main board contains the following elements:
16 digital inputs
24 digital outputs
6 PT 100 inputs
7 Inputs of 4 20Ma to read pressure/sensor for another 4-20Ma
voltage inputs for the electrode for water level
2 analog outputs of 4 20mA
Serial ports
CPU or a number of CPUs.
At least 2 ADC's for reading temperature and pressure sensors.
Each ADC maintains at least 3 temperature sensors (PT100),
( ) and 3 pressure sensors (4 ( 20mA). )
All connections to the main board are via rigid contactors for quick, efficient and dismantling and transport.

30
INPUT/OUTPUT CONNECTION TO VALVES

POWER INPUTS
GND,24VDC,5VDC OUTPUTS 1-8

DIGITAL INPUTS 1-8

OUTPUTS 9-16
DIGITAL INPUTS 9
9-16
16

PRESS INPUTS

OUTPUTS 17-24
TEMP INPUTS PT-100-1,2

TEMP INPUTS PT-100-3,4

TEMP INPUTS PT-100-5,6

24VDC DELAY
POWER & COMMUNICATIONS
ELECTRD INPUTS
24VDC INPUT

COMPUTER

SLAVE PANEL

MASTER PANEL

31
32
33
 T
S

N
R

GND
1
1
1
1
1
CONTACTOR DIIL00M OVERLO
OAD 0..4A

3x415V\50Hz

CB1
1 4 1
1
T1 L1
2
3 5 O.L. 2
T2
2 L2
4
5 6 3

2
T3
3 L3
6
A1
A2
1
1

K1
2
1

VACUUM PUMP
S1
KEY SW

AC IN

AC OUT
SSR
AC Power Connection

VDC
-
+
3-30
S2

VACUUM PUMP
4
3
SAFETY SW

6A
CB1

3
2

24V
2
5V ANL IN
1

GND
L
L

N
N

OPTION
PANEL2
PANEL1
1

COMPUTER
ANL OUT
6
PU65-14
PU30-10

5
POWER SUPPLY
POWER SUPPLY
5V

24V

4
GND
GND

POWER OUT 24VDC

3

OUTPUT24
2

PT100 SENSOR

OUTPUT23
1

OUTPUT22 24V

4-20mA
OUTPUT21
TEST POINTS

4-20mA
OUTPUT20
4-20mA

OUTPUT19 4-20mA

4-20mA
OUTPUT18
4-20mA
OUTPUT17
4-20mA
OUTPUT16
INPUT16

OUTPUT15 INPUT15

INPUT14
OUTPUT14
INPUT13
OUTPUT13
INPUT12

OUTPUT12 INPUT11

INPUT10
OUTPUT11
INPUT9
OUTPUT10
INPUT8
DIGITAL OUTPUTS

OUTPUT9 INPUT7

OUTPUT8 INPUT6
DIGITAL INPUTS

INPUT5
OUTPUT7
INPUT4
OUTPUT6
INPUT3

OUTPUT5 INPUT2

INPUT1
OUTPUT4
GND
OUTPUT3 GND
5V
CAT2004

OUTPUT2 5V
2

24V
OUTPUT1 GND
34
 3
24V

2
5V

ANL IN
1
GND
D

OPTION
PANEL2
PANEL1
1

COMPUTER
ANL OUT
Digital Input

6
5
4
3
2

PT100 SENSOR
1
24V

4--20mA

4--20mA

4--20mA

4--20mA

4--20mA

4--20mA

4--20mA

INP
PUT16

INP
PUT15

INP
PUT14

INP
PUT13
LED

INP
PUT12
TO MICROPROCESSOR
INPUT3

INP
PUT11

INP
PUT10

IN
NPUT9
470
R

IN
NPUT8
VCC
DIGITAL

IN
NPUT7

IN
NPUT6
ISO1

OPTO ISOLATOR
24VDC

3.3K

IN
NPUT5

IN
NPUT4
INPUTS

IN
NPUT3
3

IN
NPUT2
Lim
mit door1 switc
ch
IN
NPUT1

GND
GND
5V
CAT2004

5V
24V
35

GND
 3
24V

2
5V

ANL IN
1
GND

OPTION
PANEL2
PANEL1
1

COMPUTER
ANL OUT
PT100 Input

6
5
4
3
2

PT100 SENSORS
PT100--1 CONDE
ENS
RED
RED
YELOW

1
24V
4-20mA

100
R53
4-20mA

2
1 2 4-20mA
4-20mA

10K
R24
4-20mA
VCC

1 2 1 2
4-20mA

200
10K

R30
R25

4-20mA

1
2 INPUT16

10K
R55
10K
R28

2
1

INPUT15
2 1
12
13

INPUT14
2

R57
+
-

825K

INPUT13
R22
825K

U12D
1

LM324

INPUT12
14

INPUT11

INPUT10

INPUT9

INPUT8
DIGITAL

INPUT7

INPUT6

INPUT5
TO MICROPROCESSOR

INPUT4
INPUTS

INPUT3

INPUT2

INPUT1

GND
GND
5V
CAT2004

5V
24V

GND
36
 3
24V

2
5V

ANL IN
1
GND

OPTION
PANEL2
PANEL1
1

COMPUTER
ANL OUT
6
5
4
3
2

PT100 SENSOR
1
Pressure transducer Input

24V

8
4-20mA

4-20mA

4-20mA

4-20mA
24VDC

4-20mA

4-20mA
PR
RESS
4-20mA CH
HAMB
1

INPUT16

INPUT15

INPUT14

INPUT13

1 2 INPUT12
R9
120

INPUT11
3
2
+
-

INPUT10

INPUT9
U11A

1
LM324

INPUT8
DIGITAL

INPUT7

INPUT6

INPUT5

INPUT4
INPUTS

INPUT3

INPUT2

INPUT1
TO MICROPROCESSOR

GND
GND
5V
5V
24V
GND
37
 3
24V

2
5V

ANL IN
1
GND
D
Inputs Connection

OPTION
PANEL2
PANEL1
1

COMPUTER
ANL OUT
6
RED

5
RED PT100-3 COO
OLING
YELOW
DRAIN

POWER OUT 24VDC

3
OUT
TPUT24
RED

2
RED PT100-2 COND
DENSE
YELOW
RED

PT100 SENSOR
OUT
TPUT23

1
RED PT100-1 COND
DENSE
YELOW

OUT
TPUT22 24
4V
8

4-20m
mA
OUT
TPUT21

TEST POINTS
4-20m
mA PRESS DOOR
R1
OUT
TPUT20 SEAL
4-20m
mA

OUT
TPUT19 4-20m
mA
4

PRESS
4-20m
mA
JACKET
OUT
TPUT18
4-20m
mA
2

PRESS
OUT
TPUT17 CHAMBER
4-20m
mA
1

OUT
TPUT16
INPUT
T16

OUT
TPUT15 INPUT
T15

INPUT
T14
OUT
TPUT14
INPUT
T13
OUT
TPUT13
INPUT
T12
DIGITAL

TPUT12
OUT INPUT
T11

INPUT
T10
2

OUT
TPUT11 Safe
ety door1 switch
h
INPU
UT9
OUT
TPUT10
INPU
UT8
OUTPUTS

OU
UTPUT9 INPU
UT7

OU
UTPUT8 INPU
UT6
6

Flo
oat vacuum pump
p switch
DIGITAL INPUTS

INPU
UT5
OU
UTPUT7
INPU
UT4
OU
UTPUT6
INPU
UT3
3

Lim
mit door1 switch
OU
UTPUT5 INPU
UT2

INPU
UT1
OU
UTPUT4
GN
ND
OU
UTPUT3 GN
ND
5V
5
CAT2004

OU
UTPUT2 5V
5
24
4V
OU
UTPUT1 GN
ND
38
 3
24V
2

5V
ANL IN
1
Valves Connection

GND
2

OPTION
PANEL2
PANEL1
1

COMPUTER
ANL OUT
6
5

24VDC
4

POWER OUT 24VDC

3

OUTPUT24
2

PT100 SENSOR

OUTPUT23
1

2 1 OUTPUT22 24V
2

53-1
Vac
cuum from gas
sket door1
4-20
0mA
OUTPUT21
TEST POINTS

4-20
0mA

34-1
4

2 1
Steam to gask
S ket door1 OUTPUT20
4-20
0mA
3

OUTPUT19 4-20
0mA
39-1

2 1
2

Clos
se door1 0mA
4-20
OUTPUT18
4-20
0mA
1

OUTPUT17
38-1

2 1 4-20
0mA
Ope
en door1 OUTPUT16
INPU
UT16

OUTPUT15 INPU
UT15

INPU
UT14
OUTPUT14
INPU
UT13
12

2 1 OUTPUT13
Feed wate
er -cooling fas
st exhaust INPU
UT12
DIGITAL

OUTPUT12 INPU
UT11
75

2 1 INPU
UT10
3

Exhaust to vacuu
um pump OUTPUT11
INPUT9
OUTPUT10
74

INPUT8
Slow exhaustt to drain
S 2 1
1
OUTPUTS

OUTPUT9 INPUT7
8

OUTPUT8 INPUT6
DIGITAL INPUTS

91

Steam inlet to
o jacket 2 1
7

INPUT5
OUTPUT7
INPUT4
6

OUTPUT6
93

2 1 INPUT3
Ste
eam inlet to chamber
5

OUTPUT5 INPUT2

INPUT1
43

2 1 OUTPUT4
Filtered a
air inlet GND
G
OUTPUT3 GND
G
5V
15
2
CAT2004

2 1
Wa
ater to vacuum
m pump OUTPUT2 5V
24V
2
OUTPUT1 GND
G
39
 T
S

N
R

GND
1
1
1
1
1

PKZM0-4

DIL00AM
CONTACTOR
CB1
OVERLOAD
3x208V/60Hz

1 4 1
2 T1 L1
L
3 5 O.L. 2
4 T2 L2
L
5 6 3
6 T3 L3
L
A1
A2

K1
S1

VACUUM PUMP
2
1
1
1
2
CB1 6A

2
1
L
L

N
N

AC IN

AC OUT
SSR

VDC
-
+
3-30

VACUUM PUMP
4
3
PU65-14
PU30-10

1
POWER SUPPLY
POWER SUPPLY
5V

24V

GND
GND

24V
RS112

4
2
2

5V
ANL IN
1

GND
2

OPTION
PANEL2
PANEL1

COMPUTER

53-2
ANL OUT
1

2 1
Vacuum from
m gasket door2
6

RED
34-2

PT100-4 C
COOLING DRAIN
5

RED
Air to gasket door2 2 1 YELOW
POWER OUT 24VDC
4

RED
PT100-3 C
CHAMBER
3

RED
39-2

OUTPUT24 YELOW
2 1
8

Close door2 RED

PT100-2 C
CONDENSE
2

RED
YELOW
OUTPUT23 RED
PT100 SENSOR

PT100-1 C
CONDENSE
7
1

RED
YELOW
38-2

Open door2 2 1
OUTPUT22 24V
6
8

PRESS DO
OOR2
4-20mA SEAL
OUTPUT21
5
TEST POINTS

53-1

2 1 4-20mA
Vacuum from
m gasket door1 PRESS
OUTPUT20
4

4-20mA EAL
DOOR1 SE
5

OUTPUT19 4-20mA
34-1
3
4

2 1 PRESS
Air to gasket door1
4-20mA JACKET
OUTPUT18
2

4-20mA
2

39-1

PRESS
2 1 OUTPUT17
1

Close door1 4-20mA CHAMBER

R
1

OUTPUT16
8

INPUT16
38-1

Open door1 2 1
OUTPUT15 INPUT15
7

INPUT14
OUTPUT14
11

INPUT13
Feed water - co
ool jacket 2 1
OUTPUT13
INPUT12 Safety door2 s
switch
DIGITAL

OUTPUT12 INPUT11
98
4
3

BioSteam
B 2 1

INPUT10 Safety door1 sw

witch
2

OUTPUT11
3

INPUT9
31

2 1
Compre
essed air OUTPUT10
INPUT8
OUTPUTS

OUTPUT9 INPUT7
12

Limit door2 sw
witch
1
7

Feed water -cooling fast exhaust 2 1

OUTPUT8 INPUT6 Float vacuum p

pump
8
6

DIGITAL INPUTS

switch
INPUT5
75

2 1 OUTPUT7
7

Exhaust to vacuu
um pump
INPUT4
OUTPUT6
6

INPUT3
74

Limit door1 sw
witch
3

2 1
Slow exhaustt to drain
OUTPUT5 INPUT2
5

Size
Title

INPUT1
91

A2

Date:

2 1 OUTPUT4
4

Steam inlet to jacket

GND
OUTPUT3 GND
3

93
CAT2004

2 1 5V
Steam inlet to
o chamber
OUTPUT2 5V
2

Document Number

24V
H02SU01BH112
43

2 1 OUTPUT1 GND
Filtered
d air inlet
Thursday , January 06, 2005
58

Jack
ket drain 2 1
Sheet
1
33

2 1
oling pipe
Splash coo
of
1
15

2 1
Electric control horizontal autoclave with two sliding doors

Water to vacuu
um pump
1
Rev

40
41
CONTROL AND MONITORING

42
4.1 Description of Panel CAT 2007
The Operation Panel is composed of the following parts:
4 rows display, 20 characters in each row.
18 key pads
2 LED's (Fail, Run)
1 locking
l ki kkey
4.1.1 Keypad
STANDBY

The ON/ST.BY key is located on the top right side of the panel. Pressing the keypad lights up the operation panel.
Wh there
When th are 2 panels,
l bboth
th lilight thoughh onlyl one was pushed.
ht up even th hd
The 3 Command Keypads
F1-3: Functions 1-3

F1 F2 F3
These keypads are found under the display. The function to be performed will be listed on the display above the keypad.

43
10 Digit keypads
These keypads are used for 2 purposes.
In normal working conditions, the program option is used pressing on the number keypad, for example no. 3 selects program
3.
Under display mode, the keypads will be used as number keypads to enter a code or a number.

4.3.4 Changing Cycle Parameters

The screen that allows for parametric changes appears as follows:

Pressingg Clear or Enter before enteringg the code returns to the Stand Byy screen,, and the RUN & FAIL LED's are lit.
Enter the correct code only via the UP/DN keypads, press ENTER to proceed to the next screen.
Pressing the UP/DN keypads can also change all parameter values.
Cycle Setup Parameters:
Code 1 or 11 Parameters

44
45
46
47
48
49
50
51
52
Steam Generator

78
Steam Generation

79
ELECTRICAL CONNECTION DIAGRAM
G-PROTEC-V1
DIGITAL SIGNAL
FOR CONTROL
SYSTEM

JP5
2
1
HEADER 2 JP9
4
JP2 3
LED1 2
1 HEADER 4 1
2
3 JP10
LED8 4
HEADER 3
3
2
LED2 HEADER 4 1

JP1
LED3 6
5
JP4 4 LED1 SHORT ELECTRODE TOUCHES THE WATER LEVEL
LED4 3 LED8 HIGH PRESSURE ( JP10 SENSOR )
3 2 LED2 THERMOSTAT - OVER TEMPERATURE INDICATION
2 HEADER 6 1 LED3
3 PRESSOSTAT1
1 - OVER PRESSURE INDICATION
1 LED5 LED4 PRESSOSTAT2 - OVER PRESSURE INDICATION
JP7 LED5 NO WATER IN GENERATOR
HEADER 3 2 LED6 HIGH TEMP. ( THERMO COUPLE )
LED6 HEADER 2 1 LED7 HEATER CAN BE OPERATED

LED7

80
Electrical Drawing 36 kW

81
82
Limit Control Pressure Switch
To set the cut-off pressure, turn the bronze-colored pressure switch knob. Turn it
clockwise to decrease pressure and counter-clockwise to increase the pressure.
The pressure switch will be set to a cut-off
cut off pressure of 5 Bars.
Bars

Reset
lever
Turning off
pressure

Cut-off
pressure Differential
pressure

83
Door Type

84
Vertical Sliding Door

2
Legend
1Oil tank stick
3 gauge
2Oil tank
3Door operating piston
4Lock valve
4 5Oil refilling connection
6Door speed adjustment
valve
5

85
86
87
88
88
89
General Information

90
Steam Graph
(Temperature\ Pressure)

91
Steam Tables Steam Tables
Imperial Units Metric SI Units

92
 Preessure Teemperature table for satturated steam
m

93
 Pressuree Temperrature table for saturated steam

94
95
96
 PT1100 Resistannce Table

97
 Whatever your application may be, Tuttnauer makes the right
At l
Autoclave with
ith th
the right
i ht capacity
it !

Tuttnauer Europe b.v. Tuttnauer USA Co. Ltd.

Paardeweide 36 P.O.B. 7191
4800GD Breda, The Netherlands
Tel: (31) 76 5423510,
Fax: (31) 76 5423540
Email: tuttnauer@tip.nl
www.tuttnauer.com
98
25 Power Drive
Hauppauge, NY,11788,USA
Tel: (631) 737 48 50, (800) 624 5836
Fax: (631) 737 07 20
Email: info@tuttnauer.com
www.tuttnauer.com

98