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Interlab G26
Users Manual
Ver. 2.0 December 2010
TABLE OF CONTENTS
1. GENERAL WARNINGS............................................................ 6
1.1 Safety measures .................................................................. 7
1.1.1 Electrical safety ...................................................................... 7
1.1.2 Fire safety............................................................................... 7
1.1.3 Mechanical safety ................................................................... 7
1.1.4 Biological and chemical safety ............................................... 8
1.2 Warning labels on the instrument ......................................... 9
1.3 Interferences and limitations ............................................... 10
1.4 Responsibility of INTERLAB ............................................... 10
1.5 Trademarks ........................................................................ 10
1.6 Identification plate .............................................................. 11
2. INTRODUCTION..................................................................... 12
2.1 Purpose and contents of the manual .................................. 12
2.2 Keeping the manual............................................................ 12
2.3 Guide to reading ................................................................. 13
4. INSTALLATION ...................................................................... 26
4.1 Operating environment ....................................................... 26
4.1.1 Environmental requirements................................................. 26
4.1.2 Minum configuration system ................................................. 26
4.2 Unpacking and checking .................................................... 27
4.2.1 Unpacking instructions ......................................................... 27
4.2.2 Check accessory box contents ............................................. 30
TABLE OF CONTENTS
2_________________________________________________________________________________
5. STARTING .............................................................................. 54
5.1 The starting of the instrument ............................................. 54
5.1.1 The filling of the external tanks ............................................. 54
5.1.2 Preparation of the stain tanks ............................................... 57
5.1.3 Positioning of the applicators................................................ 59
5.1.4 Preparation of the sample plate support ............................... 60
5.1.5 Preparation of the migration chamber .................................. 61
5.1.6 Preparation of the gel ........................................................... 63
5.1.7 Preparation of the racks ....................................................... 67
5.2 Preliminary controls before starting .................................... 69
Read this entire manual before installing and operating the instrument.
Do not use the instrument if you do not have understood all the information
in this manual. For any doubt please contact the Technical Service Interlab.
Do not use this manual if the pages are missing. If so Interlab disclaims any
liability for damages, performance does not meet the features stated or
erroneous results.
The device was designed and constructed to ensure, during normal use,
an adequate level personal and collective security, in accordance with
existing rules. Be careful, however, the conduct rules here below.
In case of replacing the fuses, use the same type and amperage
value as indicated on the identification plate.
Label Description
Moving parts
Risk of
electric shock
Risk of
burns
Risk of
biological contamination
Interlab can not be held liable for damages to persons or property, validity and
reliability of the results obtained, the characteristics reported for the
instrument, if:
We do not observe the safety rules.
The installation, adjustment, alterations and repairs are carried out by
unauthorized personnel from INTERLAB.
The operating environment where the equipment is installed does not
comply with the requirements in this manual.
The electrical system in the room where the equipment is installed is
not lawful in the country and its safety guidelines.
The instrument is not used in accordance with the instructions in this
manual.
The manufacturer, installer or importer can not be held responsible for any
loss data stored or disclosure sensitive personal data.
1.5 TRADEMARKS
Purpose
The purpose of the manual is to allow operators instrument to take those
measures and provide all necessary human and material resources for its
proper use and safe.
Contents
This manual contains all the information necessary for the installation,
operation and maintenance of the instrument.
The scrupulous observance of what it is described guarantees a high degree
safety and productivity of the instrument.
Deterioration or loss
If you need to apply for another copy to the Distributor reference or
INTERLAB.
2. INTRODUCTION 13
__________________________________________________________________________________________
In this manual the points of great importance are highlighted symbols below.
Traders are advised to consult the important installation instructions and
operating instructions contained in this document and especially to observe
directions, regulations and prohibitions identified the following symbols:
WARNING
CAUTION
NOTE
With this symbol is expressed as a note indicating the optimal use of the
facility are important reminders.
The G26 INTERLAB by the separation of different protein fractions using the
principle of electrophoresis, that the difference in mobility between electrically
charged molecules that orient and migrate with different rates when subjected
to an electric field. The migration is performed at constant temperature,
obtained through the use of Peltier effect modules on agarose gel plates
containing a specific buffer solution according to the method to execute. The
gel acts as a support and molecular sieve and allows the different fractions
could be resolved on the basis of net charge possessed. After electrophoresis,
the gel is dried at a prescribed temperature, developed by appropriate
staining, destained and dried. The qualitative and/or quantitative colorimetric
detection occurs through the migration of the bands of fronts. For methods that
also allow the quantitative assessment, the measurement method is based on
optical densitometry reading of a source diode LED and a photodiode
detector. The signal obtained is sent to the PC to be processed. The
management of all phases of the analytical procedure is done through a
software system firmware factory installed on the instrument and a software
user interface installed on a PC that also returns the results of the analysis
and allows the processing and storage.
The INTERLAB G26 is a new-generation device that allows, for the majority of
analytical methods, to run in full automation, the various stages of clinical
electrophoresis on agarose gel, minimizing operator intervention. The system,
composed of the instrument for analysis and related software
ELFOLAB / INTERLAB G26 installed on Personal Computer, allows to perform
full automation in the various stages of the analytical procedure.
3. THE INTERLAB G26 INSTRUMENT 15
_________________________________________________________________________________
INTERLAB G26 without the sampler that runs the following steps:
dispensation of samples on agarose gel;
electrophoretic migration;
drying the gel;
gel staining;
gel destaining;
gel drying;
densitometric reading of the gel;
acquisition and data processing.
INTERLAB G26 with the sampler that runs the following steps:
identification of the tubes by reading a bar code;
sampling of samples from tubes;
deposit of samples in the sample plate;
dispensation of samples on agarose gel;
electrophoretic migration;
drying the gel;
gel staining;
gel destaining;
gel drying;
densitometric reading of the gel;
acquisition and data processing.
The system has great flexibility, allows a rational and efficient management of
time for analysis, with significant increase in speed of analysis and the ability
of the machine, thanks to:
- High degree automation;
- The wide variety of programs you can use;
- The various options you can use;
- The ability to schedule work sessions for the development of methods in
order of priority that can be changed if necessary;
- The possibility of processing methods in parallel, taking advantage of
downtime from one phase to the next.
With the INTERLAB G26, you can execute with complete automation, the
following analysis:
Protein electrophoresis of serum and of concentrated urines;
1-2 protein electrophoresis of serum and of concentrated urines;
Protein expanded electrophoresis of serum and of concentrated urines;
1-2 protein expanded electrophoresis of serum and of concentrated
urines;
Electrophoresis of the proteins in High Resolution;
Electrophoresis of the concentrated proteins in High Resolution;
NOTE
All methods perform with the instrument are described individually in
detail the procedures manual.
Interface to PC RS232
Througt modules
Temperature control during the migration
Peltier effect
UPC / EAN,
UPC / EAN Supplemental,
UCC / EAN 128, Code 39,
Code 39 Full ASCII,
TriOptic Code 39,
Types of bar codes read by the instrument Code 128,
(only instrument with sampler) Code128 Full ASCII,
Codabar, Code 2 of 5,
Codice 2 of 5 Interleaved,
Code 93, MSI, Code 11,
IATA, RSS Variants,
Chinese Code 2 of 5
Weight 45 kg
Temperature:
18C to +28C
Analysis conditions
Relative Humidity:
5% to 85% not condensing
Temperature:
0C to +40C
Operating conditions
Relative Humidity:
5% to 85% not condensing
Temperature:
da 0C a +50C
Storage conditions
Relative Humidity:
5% to 85% not condensing
Noise level 52 dB
Ricycling
3.4 DESCRIPTION
3.4.1 OUTSIDE
Cover
Hood
Identification plate
Switch ON/OFF
Serial port
Jack plugs
3.4.2 INSIDE
Mechanical arm
Driven by system software, couplet by means an eletromagnet and moves
during the various stages of the analytical procedure: the needle dispenser,
applicators, the holder of the agarose gel, the lids of the tanks of the dyes.
Needle dispenser
Take samples from tubes housed in racks and rack dispenses them in
individual wells of the plate sample holder.
Parking applicators
This is the site of the applicators.
Oven
Here we begin the process of drying the gel at a temperature between 70 and
80 C using hot air.
Optical drive
Through a diode LED and a photodiode, the readings at the end gel
densitometry of the whole analytical procedure. The relevant data collected
will be transferred to a PC for processing.
Parking gel
It is the place of the two frames where gel holder is taken at the beginning of
the analysis and deposited at the end.
Plate migration
The plate agarose gel adheres on it by creating the vacuum and the following
three phases:
dispensing of samples on the gel through the applicators;
electrophoretic migration through an electric field;
drying of the gel.
Use the Peltier effect to keep temperature constant during any phase of
electrophoretic migration and drying.
Applicators
Through the three applications 13 samples are taken from the plate sample
one by one and are applied on the gel. After each application, the applicator is
decontaminated by washing in the place.
Migration chamber
Here there are the slots with the platinum electrodes where to place the
sponges soaked in electrolyte solution in order to conduct the current in the
gel. After the sampling on the gel, the migration plate rotates 180 degrees
clockwise by closing the migration chamber where the vacuum will be created.
The conductive gel plate will adhere to the sponges and the gel will be
subjected to the potential difference that will cause the electric field for
electrophoresis.
Racks 1, 2, 3
It consists of 3 rows of 13 holes each in which there are the tubes with the
samples. Through a bar code printed on each tube, the system is able to read
the patient information and the type of analysis to read and transmit data to
your PC.
To ensure optimum operation of the instrument and the reliability of the results
follow the instructions below.
CAUTION
Follow the instructions below for unpacking the instrument. Inspect carefully
the instrument and accessories in the box enclosed within the same
instrument. In case of damages found or accessories are missing, contact the
Reference Distributor or INTERLAB.
Remove the four L-shaped metal fasteners that hold the outer casing
of cardboard to the wooden base. Use a screwdriver inserted
between the stopper and the base metal and force it downwards until
the single firm has dropped.
Then lift up the casing of cardboard to remove it, as indicated by four
arrows in the figure.
NOTE
Keep the original packaging for a possible transport of the
instrument.
Pull in the direction indicated by arrows the two auction block to free
the instrument from the base of the packaging.
Place the instrument in the installation site and remove it from
protection.
CAUTION
SAE909M Applicators 3
Racks 3
Tank
SAE912M STAIN 1
SOLUTION 1
Tank
SAE913M STAIN 1
SOLUTION 2
Tank
SAE914M STAIN 1
SOLUTION 3
Whole tank
SAE915M WASHING 1
SOLUTION
Whole tank
DISTILLED 1
WATER
Whole tank
SAE916M DESTAIN 1
SOLUTION
Diluent container
1
for 2 ml
SAE919M Holders 2
Support sample
SAE920M 1
holder
SAE140M Mousepad 1
Handles 4
4.3.1. LOCATION
Place the instrument on a flat horizontal surface of minimum size
150x70 cm, clean, stable and vibration-free, at a height of about 85
cm above the floor so the operator can perform the normal operating
procedures easily.
Check the horizontality of the instrument through a spirit level.
Check around the instrument there is a minimum clearance of 15 cm
to allow the air circulation for cooling.
Place the instrument to make easier the connection and
disconnection operations of external accessories (solution tanks and
waste tanks) and electrical connections.
WARNING
Connect the two pipes of the distilled water tank to the hydraulic
connections marked by the label DISTILLED WATER. Connect the
jack to level control at one of the three jack plugs.
Connect the pipe of the washing solution tank to the hydraulic
connection marked by the label WASHING.
Connect the pipe of the destain solution tank to the hydraulic
connection marked by the label DESTAIN SOLUTION.
CAUTION
4.3.3. PC CONNECTION
Connect by RS232 cable supplied with the computer's serial port to
the serial port of the instrument on the right side.
WARNINGS
1. Enter the Control Panel from the Start Menu low in the left
1. Enter the Control Panel from the Start Menu low in the left .
1. Enter the Control Panel from the Start Menu low in the left.
5. Click OK.
NOTE
Before reainstalling or installing an update, you must follow a
procedure to uninstall the previous version before loading
again Elfolab or Interlab G26 on your computer.
Installation procedure
7. Choose the installation directory (already set) and press the button
with the icon PC.
8. Click Continue.
5. Click Continue.
7. Click OK.
NOTE
Uninstalling Elfolab and/or Interlab G26 does not delete the
database work.
WARNINGS
During the use of the instrument and handling its internal and
exterior accessories be careful to wear all personal protective
equipment (gloves, goggles, laboratory gown and surgical)
Pay attention before disconnecting the biological waste and
its accessories.
CAUTION
CAUTION
The procedures described below are common to all methods they should be
carried out completely the first time when the instrument is in use. Some
procedures must be chosen according to the methods that you intend to run
and repeat as needed.
Replace the cap clockwise and screw the green ring nut.
IMPORTANT: Replace the stain solution of the tank 2 after five rounds
of staining (5 treated gel plates).
It is not necessary to fill all the three tanks if you do not have to follow
all the methods above mentioned. You can also use the instrument only
with one or two tanks necessary to perform the methods.
WARNINGS
The blade applicators are sharp. Do not touch them with your
fingers to avoid injury.
The applicators come into contact with potentially infectious
samples: biological hazards.
Withdraw from the kit of the method to run the sample plate
disposable B.
Prepare the sample holder support by placing in the site C the
sample plate B by means of the grid and lock mounting as showed in
the figure on the left.
Insert the prepared sample plate support in the site on the left side of
the instrument, pushing it to the end.
Insert the migration chamber in the site located at the center of the
instrument, slightly tilted forward.
Push it forward until it stops, then, down slightly until you hear a click
indicating the correct position.
Withdraw from the kit of the method to run the agarose gel plate and
a blotting paper. The plates are disposable and ready for use.
Place the plate on a clean sheet of paper turning up on the top with
the gel. The top enables the reading of silk screen printed on the gel
plate.
Run through the blotting paper the absorb excess of the buffer
solution, making sure that the blotting paper adheres to the entire
surface of the plate.
Take the gel plate, holding the palm of your hand from both ends,
remove the blotting paper quickly.
IMPORTANT: Do not leave for too much time the blotting paper on the
gel to avoid its excessive dehydration.
Insert the gel plate gently in the holder, slide it into the appropriate
guide rails. To advance it is necessary to impress a slight upward
curve and apply a little pressure forward.
Finally check the gel plate is properly positioned between the rails of
the holder.
Insert the sample tubes into the holes of the rack turning toward the
opening of each hole in order to read the bar code label of the tubes.
Place the racks according to the relation between the number of the
rack and the site. Do not try to reverse the position of the racks.
Place the racks at a constant speed and taking at least 5 seconds to
allow the reading of the bar codes on the tubes.
Push each rack in the bottom of the site until you hear the magnetic
block.
Make sure that in the tanks of distilled water, washing solution and destain
solution there is a sufficient level of the liquid.
Check the fluid level in the tanks of waste and biological waste; replace
them if they are full.
Check the level of the stain solutions for the required methods through the
little window on the front of each tank. Check that any liquid level is
between the two notches MIN and MAX.
Check the three applications are properly inserted in their parking.
Make sure the dispenser needle is located correctly in its parking.
Check the sample plate support is properly inserted and locked.
Check the migration chamber is properly prepared, inserted and locked.
Make sure the racks have been properly prepared by placing the tubes in
order to allow the reading of the bar codes. Make sure each rack is
inserted and locked.
The database is initially empty, to open it and enter data for new
patients or working on the day or the historical part, follow these
instructions:
1. Click on the Database and select from the men the Open
Database:
delay this step later, if so, just click on the button or click Select
Methods to reopen this window.
NOTE
Database: Each patient has a record with the identification
data, the graphs and the values of the analysis made and all
the notes.
The main panel shows the application of the method sessions that have
been previously selected (See 6.1.2), the parameters of the methods
to start the working and a field for the choice of options. Before
restarting the methodology you must select the following options.
1. Choose from the options the method (only the instrument with the
sampler):
Sampling + Analysis For reading the bar codes, for
sampling and for starting the
analysis.
The system can read the bar codes from the tubes, retransmit the ID to
the database of patients and perform the sampling.
If you choice one of the three options: Sampling+Analysis, Sampling
or Scanning by the main panel of Elfolab G26 (See 6.2.2) then on the
session starting, it opens the window for reading the bar codes. In each
field the ID code, read by the tubes, will be displayed The number of the
fields displayed depends on the chosen method.
When the window opens, all the fields will be selected by default; in this
case after inserting the racks a green light will turn on for each bar code
read correctly.
You can also get only the ID of some tubes; in this case deselect the
fields
ID insertion manually
Duplicated ID
If one or more ID are equal, the box will become red and a dialogue
window will invite you to check the correct combination before starting
the sampling.
Start sampling after the correct combination between the read ID and
the printed ID on the tubes, by clicking No or Yes to check it.
When the volume of the liquid in the tube is less than 300 L the
following window appears:
To start the analysis click on the button Start Analysis with only
dispensed samples.
NOTE
After reading the bar code you have only just 3 minutes to
start the sampling. Otherwise the window will close without
sampling.
6.3.1 SETTINGS
Generals
Diluitions
The dilution ratios for the IFE methods and ALP isoenzymes
electrophoresis are by default. They can be modified and saved for any
use through the following window:
6.3.2 LANGUAGE
It is possible to set the language from the list.
CAUTION
Contact us
The instrument runs the following steps of the methods, in an automated way:
reading of the patients ID from tubes and sampling (only for instrument
with the sampler);
application of samples on the gel plate;
electrophoretic migration;
gel drying on the plate Peltier;
gel staining;
gel destaining;
gel drying;
densitometric reading.
7. Select the options of the analysis startup and begin the session
by clicking on the button SPE 5F.-13 (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with sampler). (See
5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After the migration take the holder with the gel from the
instrument and run manually by using the Easy Mask for the
external steps: application of the fixative solution and the
antiserum, incubation of the gel, gel blotting.
11. Click on the button Restart and wait until the end of the
analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.
11. Click the Restart button and wait until the end of the analysis.
The instrument runs the following steps of the methods, in an automated way:
reading of the patients ID from tubes and sampling (only the instrument
with the sampler);
application of samples on the gel plate;
electrophoretic migration;
gel drying on the plate Peltier;
gel staining;
gel destaining;
gel drying;
densitometric reading.
7. Select the options of the analysis startup and begin the session
by clicking on the button Hemoglobins (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
7. Select the options of the analysis startup and begin the session
by clicking on the button Acid Hemoglobins (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
The instrument runs the following steps of the methods, in an automated way:
reading of the patients ID from tubes and sampling (only the instrument
with the sampler);
application of samples on the gel plate;
electrophoretic migration;
gel drying on the plate Peltier;
gel staining;
gel destaining;
gel drying;
densitometric reading.
7. Select the options of the analysis startup and begin the session
by clicking on the button Lipo 13 (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After drying take the gel holder from the instrument and clean
the reverse part of the gel plate with cotton wool soaked in
ethanol.
11. Click on the button Restart and wait until the end of the
analysis.
7. Select the options of the analysis startup and begin the session
by clicking on the button Lipo 26 (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After drying take the gel holder from the instrument and clean
the reverse part of the gel plate with cotton wool soaked in
ethanol.
11. Click on the button Restart and wait until the end of the
analysis.
7. Select the options of the analysis startup and begin the session
by clicking on the button LDH Isoenzymes (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After the migration take the holder with the gel from the
instrument and by using the Easy Mask run the external steps
manually.
11. Click on the button Restart and wait until the end of the
analysis.
7. Select the options of the analysis startup and begin the session
by clicking on the button CPK Isoenzymes (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After the migration take the holder with the gel from the
instrument and by using the Easy Mask run the external steps
manually.
11. Click on the button Restart and wait until the end of the
analysis.
7. Select the options of the analysis startup and begin the session
by clicking on the button ALP Isoenzymes (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After the migration take the holder with the gel from the
instrument and by using the Easy Mask run the external steps
manually.
11. Click on the button Restart and wait until the end of the
analysis.
7.6 CRYOGLOBULINS
The methods for the cryoglobulins carried out by the instrument INTERLAB
G26 are made by semiautomatic procedures.
7. Select the options of the analysis startup and begin the session
by clicking on the button Cryo 2 (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After the migration take the holder with the gel from the
instrument and run the external steps manually.
11. Click on the button Restart and wait until the end of the
analysis.
7. Select the options of the analysis startup and begin the session
by clicking on the button Cryo 4 (See 6.2.2).
8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.
9. After the migration take the holder with the gel from the
instrument and run the external steps manually.
11. Click on the button Restart and wait until the end of the
analysis.
The method for the CSF isoelectric focusing, carried out by the INTERLAB
G26 instrument, has the electrophoretic migration as an unique automated
step.
Make reference to the PK135 procedure of the CD included in the kit box.
4. Select the options of the analysis startup and begin the session
by clicking on the button CSF IEF (See 6.2.2).
5. After the migration take the holder with the gel from the
instrument and run the external steps manually as described in
the procedure.
The methods for the SDS proteinurie run by the instrument INTERLAB G26
are the automated steps:
electrophoretic migration;
drying of the gel plate Peltier;
gel staining;
gel destaing;
gel drying;
6. Select the options of the analysis startup and begin the session
by clicking on the button SDS Urines (See 6.2.2).
The method for the -amylase isoenzymes run by the INTERLAB G26
instrument has the electrophoretic migration as an unique automated step.
4. Select the options of the analysis startup and begin the session
by clicking on the button Alpha-Amylase (See 6.2.2).
5. After the migration take the holder with the gel from the
instrument and run the external steps manually as described in
the procedure.
Make reference to the PK138 procedure of the CD included in the kit box.
4. Select the options of the analysis startup and begin the session
by clicking on the button Alpha 1 Anti-Tripsyn (See 6.2.2).
5. After the migration take the holder with the gel from the
instrument and run the external steps manually as described in
the procedure.
After using the instrument clean with damped paper of distilled water the
electrodes of the migration chamber.
WARNING
Soak the migration chamber in water for 30 minutes. First rinse it under
the running water, then with the distilled water and finally dry it with the
blotting paper.
Empty the tanks of the stain solutions, rinse them under the running water,
after with distilled water and finally let them dry to air before replacing
them in the instrument.
Clean the Peltier plate with a soft paper soaked in ethyl alcohol.
CAUTION
Be careful do not fit alcohol into the plate holes during cleaning the
Peltier plate.
9. PROBLEMS AND SOLUTIONS 155
_________________________________________________________________________________
Before any movement and any assistance of the Technical Support Staff, the
instrument must be disinfected in accordance with the provisions of the
disinfection Protocol .
For the waste disposal work in accordance with the current laws.
For further information on safe waste disposal, on the associated risks and the
precautions to be taken, including special protective measures, make
reference to the safety sheet of the reagents available from the Technical
Service of the supplier.
WARNING
9.1 ALARM
During the use alarm messages can be displayed on the interface PC. They refer to
a problem of the instrument or a mistake made by the operator.
In most cases you can use the dialogue window, with two or three buttons depending
on the kind of error, as shown in the table below.
Solutions
Problem Alarm Notes
Ignore Retry Cancel
Solutions
Problem Alarm Notes
Ignore Retry Cancel
The error is
Empty tube Liquid Tube insufficient. reported for
the IFE only.
The Sample Support is not
Sample Support inserted. Insert the Samples
Support and Retry.
Overtemperature The Migration Chamber has
Peltier exceeded 80 C.
Overtemperature The Drying Chamber has
drying chamber exceeded 80 C
No Liquid in the Washing
Washing needle
Tank Needle/Applicator. Fill
tank
the tank needed.
The Treatment Chamber
has not been filled. Control
Staining tank
the level of the tank and
Retry.
Solutions
Problem Alarm Notes
Ignore Retry Cancel
Solutions
Problem Error message Notes
Ignore Retry Cancel
There is only
the button "OK"
to verify that
the user has
Washing Absence Liquid in the taken what it
applicators washing tank. has appened.
The following
analysis
continue
regularly.
Cover open Cover open!
Absence Liquid of washing
Washing needle Needle. Fill the tank of
distilled water and Retry.
Internal logic
Internal logic error.
error.
Volume Required
Volume Required wrong.
wrong.
9.2 TROUBLESHOOTING
Possible
Problem Solutions
cause
Wrong insertion of the gel in the Make sure the gel is inserted in the
holder. holder correctly and restart the
analysis.
WARNING
During the sampling the needle can obstruct. In this case on the PC interface
a window will display needle obstructs.
For cleaning the needle use the iron wire and place it in a container for any
liquid seepage. The needle is POTENTIALLY INFECTED, so use all the
required personal equipment for protection and observe all the safety
measures and PAY ATTENTION.
L R
Labels .............................................. 9 Responsibility ................................. 10
Limitations ..................................... 10
Location ......................................... 34
S
M Safety
Biological and Chemical S. .......... 7
Main panel ..................................... 74 Electrical S. .................................. 8
Maintenance ................................ 154 Mechanical S. ............................... 7
Manual Fire S. .......................................... 7
M. keeping ................................. 12 Serial port ....................................... 19
Guide at M. ................................ 13 Software ......................................... 70
Purpose and contents of the M. . 12 Starting ........................................... 54
Methods ......................................... 83 System configuration ...................... 26
Migration chamber ......................... 24 Switch ............................................. 19
O T
Operation principle ........................ 14 Technical features .......................... 17
Outside instrument ........................ 19 Trademarks .................................... 10
P U
Parking Unpacking ...................................... 27
Applicator P. .............................. 21
Gel holder P. ............................. 21
Preparation W
Gel P. ........................................ 63
Instrument P. ............................. 54 Wells
Migration chamber P. ................ 61 Immunofixation diluent W. .......... 21
Outside tank P. .......................... 54 Needle washing W. .................... 21
Racks P. .................................... 67 Applicator washing W. ................ 21
Sample plate support P. ............ 60 Windows 7 ...................................... 41
Stain solution tank P. ................. 57 Windows Vista ................................ 37
Problems and solutions ............... 156