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INTERLAB S.r.l.

Via Rina Monti, 26


00155 Rome (Italy)
Tel: +39 06 22754350
Fax: +39 06 22754534
Web site: www.interlab-srl.com
E-mail: info@interlab-srl.com

TECHNICAL SUPPORT SERVICE

Tel. 06 22754350

Interlab G26
Users Manual
Ver. 2.0 December 2010
TABLE OF CONTENTS

1. GENERAL WARNINGS............................................................ 6
1.1 Safety measures .................................................................. 7
1.1.1 Electrical safety ...................................................................... 7
1.1.2 Fire safety............................................................................... 7
1.1.3 Mechanical safety ................................................................... 7
1.1.4 Biological and chemical safety ............................................... 8
1.2 Warning labels on the instrument ......................................... 9
1.3 Interferences and limitations ............................................... 10
1.4 Responsibility of INTERLAB ............................................... 10
1.5 Trademarks ........................................................................ 10
1.6 Identification plate .............................................................. 11

2. INTRODUCTION..................................................................... 12
2.1 Purpose and contents of the manual .................................. 12
2.2 Keeping the manual............................................................ 12
2.3 Guide to reading ................................................................. 13

3. THE INTERLAB G26 .............................................................. 14


3.1 Operation principle ............................................................. 14
3.2 Characteristics INTERLAB G26 .......................................... 14
3.3 Technical features .............................................................. 17
3.4 Description ......................................................................... 19
3.4.1 Outside ................................................................................. 19
3.4.2 Inside .................................................................................... 21
3.4.3 Accessories and internal placement ..................................... 24

4. INSTALLATION ...................................................................... 26
4.1 Operating environment ....................................................... 26
4.1.1 Environmental requirements................................................. 26
4.1.2 Minum configuration system ................................................. 26
4.2 Unpacking and checking .................................................... 27
4.2.1 Unpacking instructions ......................................................... 27
4.2.2 Check accessory box contents ............................................. 30
TABLE OF CONTENTS
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4.3 Instrument installation......................................................... 34


4.3.1 Location ................................................................................ 34
4.3.2 External accessory assembly ............................................... 34
4.3.3 PC connection ...................................................................... 35
4.3.4 Electricity grid connection ..................................................... 35
4.4 Elfolab / Interlab G26 software installation .......................... 37
4.4.1 Preliminary operations for users Windows Vista .................. 37
4.4.2 Preliminary operations for users Windows 7 ........................ 41
4.4.3 Elfolab program installation .................................................. 44
4.4.4 Interlab G26 application installation ...................................... 47
4.4.5 Elfolab anf Interlab G26 uninstallation .................................. 49
4.5 Instructions for transport and storage ................................. 51
4.5.1 Instructions for each movement ........................................... 51
4.5.2 Instructions for transport ....................................................... 52
4.5.3 Instructions for storage ......................................................... 52

5. STARTING .............................................................................. 54
5.1 The starting of the instrument ............................................. 54
5.1.1 The filling of the external tanks ............................................. 54
5.1.2 Preparation of the stain tanks ............................................... 57
5.1.3 Positioning of the applicators................................................ 59
5.1.4 Preparation of the sample plate support ............................... 60
5.1.5 Preparation of the migration chamber .................................. 61
5.1.6 Preparation of the gel ........................................................... 63
5.1.7 Preparation of the racks ....................................................... 67
5.2 Preliminary controls before starting .................................... 69

6. Elfolab / Interlab G26 SOFTWARE ........................................ 70


6.1 The Elfolab program ........................................................... 70
6.1.1 Elfolab starting...................................................................... 70
6.1.2 Database opening ................................................................ 71
6.2 The Interlab G26 application............................................... 74
6.2.1 Interlab G26 starting ............................................................. 74
6.2.2 The main panel..................................................................... 74
6.2.3 Bar code reading .................................................................. 75
6.3 The Interlab G26 menu ....................................................... 81
6.3.1 Settings ................................................................................ 81
6.3.2 Language ............................................................................. 82

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6.3.3 Setting Parameters ............................................................... 82


6.3.4 INTERLAB s.r.l. .................................................................... 82

7. METHODS OF ANALYSIS ..................................................... 83


7.1 Protein electrophoresis for serum and for urines ................ 83
7.1.1 Protein electrophoresis of serum and of concentrated
urines 5 fractions 13 tests..................................................... 83
7.1.2 Proteins electrophoresis of serum and of concentrated
urines 5 fractions 26 tests..................................................... 85
7.1.3 Proteins electrophoresis of serum and of concentrated
urines 5 fractions 39 tests..................................................... 86
7.1.4 1-2 Proteins electrophoresis of serum and of
concentrated urines 6 fractions 13 tests ............................... 88
7.1.5 1-2 Proteins electrophoresis of serum and of
concentrated urines 6 fractions 26 tests ............................... 89
7.1.6 1-2 Proteins electrophoresis of serum and of
concentrated urines 6 fractions 39 tests ............................... 91
7.1.7 Proteins expanded electrophoresis of serum and of
concentrated urines 5 fractions 13 tests ............................... 92
7.1.8 Proteins expanded electrophoresis of serum and of
concentrated urines 5 fractions 26 tests ............................... 94
7.1.9 1-2 Proteins expanded electrophoresis of serum and
of concentrated urines 6 fractions 13 tests ........................... 95
7.1.10 1-2 Proteins expanded electrophoresis of serum and
of concentrated urines 6 fractions 26 tests ........................... 97
7.1.11 Protein electrophoresis in Hight Resolution .......................... 98
7.1.12 Concentrated protein electrophoresis in Hight
Resolution .......................................................................... 100
7.2 Immunofixation of serum and of urines (IFE) .................... 102
7.2.1 Immunofixation of serum and of concentrated urines in
Acid Bleu 2 tests ................................................................. 102
7.2.2 Immunofixation of serum and of concentrated urines in
Acid Bleu 4 tests ................................................................. 104
7.2.3 Immunofissazione del siero e delle urine concentrate in
Blu Acido 6 test .................................................................. 106
7.2.4 Immunofixation of serum and of concentrated urines in
Acid Violet 2 tests ............................................................... 108
7.2.5 Immunofixation of serum and of concentrated urines in
Acid Violet 4 tests t ............................................................. 110
7.2.6 Immunofixation of serum and of concentrated urines in
Acid Violet 6 tests ............................................................... 112

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7.2.7 Bence-Jones immunofixation of the unconcentrated


urines 2 tests ...................................................................... 114
7.2.8 Bence-Jones immunofixation of the unconcentrated
urines 4 tests ...................................................................... 116
7.2.9 Bence-Jones immunofixation of the unconcentrated
urines 6 tests ...................................................................... 118
7.2.10 Bence-Jones immunofixation of the concentrated urines
2 tests ................................................................................. 120
7.2.11 Bence-Jones immunofixation of the concentrated urines
4 tests ................................................................................. 122
7.2.12 Pentavalent immunofixation of seum and of
concentrated urines 6 tests ................................................ 124
7.2.13 Pentavalent immunofixation of seum and of
concentrated urines 12 tests .............................................. 126
7.3 Electrophoresis of the hemoglobins .................................. 128
7.3.1 Electrophoresis of the alkaline hemoglobins ...................... 128
7.3.2 Electrophoresis of the acid hemoglobins ............................ 130
7.4 Electrophoresis of the lipoproteins .................................... 132
7.4.1 Electrophoresis of the lipoproteins 13 tests ........................ 132
7.4.2 Electrophoresis of the lipoproteins 26 tests ........................ 134
7.5 Electrophoresis of the isoenzymes ................................... 136
7.5.1 Electrophoresis of the isoenzymes of lactate
dehydrogenase (LDH) ........................................................ 136
7.5.2 Electrophoresis of CPK isoenzymes .................................. 138
7.5.3 Electrophoresis of alkaline phosphatase isoenzymes
(ALP) .................................................................................. 140
7.6 Cryoglobulins.................................................................... 142
7.6.1 Cryoglobulins 2 tests .......................................................... 142
7.6.2 Cryoglobulins 4 tests .......................................................... 144
7.7 CSF isoelectric focusing ................................................... 146
7.8 SDS proteinurie ................................................................ 148
7.9 Electrophoresis of the -amylase isoenzymes .................. 150
7.10 1-antitrypsine isoelectric focusing ................................... 152

8. MAINTENANCE ................................................................... 154


8.1 Daily closing procedure .................................................... 154
8.2 Weekly cleaning procedure .............................................. 154
8.3 Disinfection of the instrument ........................................... 155
8.4 Waste disposal ................................................................. 155

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9. PROBLEMS AND SOLUTIONS ........................................... 156


9.1 Alarms .............................................................................. 156
9.2 Troubleshooting................................................................ 160

10. INDEX ................................................................................... 161

Users Manual Ver. 2.0


1. GENERAL WARNINGS

 Read this entire manual before installing and operating the instrument.

 Do not use the instrument if you do not have understood all the information
in this manual. For any doubt please contact the Technical Service Interlab.

 Interlab reserves the right to change features and/or function of the


instrument and to amend this manual at any time without notice.

 Do not use this manual if the pages are missing. If so Interlab disclaims any
liability for damages, performance does not meet the features stated or
erroneous results.

 Maintenance instrument is restricted to authorized Interlab staff.

 The parameters analysis programs are set by default to ensure optimum


operation of the instrument and the security situation. In case of variation,
Interlab disclaims any liability for damages, performance does not meet the
specifications stated or erroneous results.

 The use of the instrument is restricted to qualified personnel only.

 Pay special attention to safety information.

 Respect the existing protocols for safety in laboratory analysis.

 Follow all warnings and notes found in other manual chapeters.


1. GENERAL WARNINGS 7
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1.1 SAFETY MEASURES

The device was designed and constructed to ensure, during normal use,
an adequate level personal and collective security, in accordance with
existing rules. Be careful, however, the conduct rules here below.

1.1.1 ELECTRICAL SAFETY


 Before connecting the power supply check the voltage and
frequency corresponding to the features device.
 The device has a cable with three conductors, one shield
(ground). Before connecting the unit to the electricity grid,
check that electrical system is fitted with an efficient ground.
 Do not remove the hood of protection if the unit is connected to
the electricity grid.
 Connect and disconnect cables only with the switch in position
0 (off).
 Do not place containers with liquids.

1.1.2 FIRE SAFETY


The fuses of the instrument are used to protect and reduce, in case of
overvoltage, a potential fire hazard.

 In case of replacing the fuses, use the same type and amperage
value as indicated on the identification plate.

1.1.3 MECHANICAL SAFETY


The movements run by the robotic arm and other moving parts are slow
and less powerful. To ensure greater safety in case opening protective
cover during the operation, all moving parts will stop after completing
the movement and during migration electrophoresis the applied voltage
is canceled.

 Do not use the device by depressing the opening safety sensor.


 Turn on the instrument when the cover is closed.
 Do not open the lid when the instrument is working.
 Before lifting the lid make sure all the moving parts are stopped.

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8 1. GENERAL WARNINGS
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1.1.4 BIOLOGICAL AND CHEMICAL SAFETY


The instrument use chemical reagents and biological samples that are
potential carriers pathogens and/or infectious. You understand and
implement all precautions required in compliance with the regulation
biological risk, in addition to what follows.

 Always handle samples as INFECTIOUS POTENTIALLY.


 Be VERY CAREFUL when handle samples and reagents.
 ALWAYS wear during any use of the instrument cover is open,
during the manual preparation of the analysis, while handling
samples and reagents, and during the closing of the waste
tanks, prescribed personal protective equipment: gloves,
goggles, lab coat and surgical mask.
 Do not use the device inhibiting the opening safety sensor that
ensures protection by cover.
 If in exceptional cases, during the sampling, it would be
necessary to open the cover, be VERY CAREFUL because the
needle is potentially infected, though only during the washing of
the needle after verifying that all the organs in movement have
been stopped and on the PC interface the message Cover
open appear. Wear all personal protective equipment required.
 Before performing any maintenance or repairing eliminate all
waste solutions in accordance with current standards of safety
regulation biological risk in the laboratory and disinfect the
instrument.

Users Manual Ver. 2.0


1. GENERAL WARNINGS 9
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1.2 WARNING LABELS ON THE INSTRUMENT

Label Description

Moving parts

Risk of
electric shock

Risk of
burns

Risk of
biological contamination

Warning for the Service


Personnel: unplug the
power supply before
working on the machine.

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10 1. GENERAL WARNINGS
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1.3 INTERFERENCES AND LIMITATIONS

The INTERLAB G26 is designed to be used with procedures established by


Interlab and components manufactured and supplied by Interlab (kit containing
agarose gel plates, shelves, sponges soaked in buffer solution, reagent
solution for staining, washing and destaining of the gel).
The optimal functioning instrument and the reliability of the results are
guaranteed only when used components supplied by Interlab and do
not observe the safety rules.
The incorrect application of the recommended procedure may affect the
results.

1.4 RESPONSIBILITY OF INTERLAB

Interlab can not be held liable for damages to persons or property, validity and
reliability of the results obtained, the characteristics reported for the
instrument, if:
We do not observe the safety rules.
The installation, adjustment, alterations and repairs are carried out by
unauthorized personnel from INTERLAB.
The operating environment where the equipment is installed does not
comply with the requirements in this manual.
The electrical system in the room where the equipment is installed is
not lawful in the country and its safety guidelines.
The instrument is not used in accordance with the instructions in this
manual.
The manufacturer, installer or importer can not be held responsible for any
loss data stored or disclosure sensitive personal data.

 It is recommended that you save important data on your hard drive as


well, even on a backup device.
 Observe the requirements of the privacy laws regarding the
processing personal data.

1.5 TRADEMARKS

Windows is a registered trademark of Microsoft Corporation.


The copyright programs Elfolab and Interlab G26 and this manual are
INTERLAB S.r.l.
Programs Elfolab, Interlab G26 and accompanying documentation are
protected by the laws on copyright.

Users Manual Ver. 2.0


1. GENERAL WARNINGS 11
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1.6 IDENTIFICATION PLATE

The manufacturer declares the product complies with EC directives on medical


devices in vitro diagnostic (IVD). Its marking plate showing the characteristic
data instrument, as the manufacturer and serial number is located on the
lower right side instrument.

Users Manual Ver. 2.0


2. INTRODUCTION

2.1 PURPOSE AND CONTENTS OF THE MANUAL

Purpose
The purpose of the manual is to allow operators instrument to take those
measures and provide all necessary human and material resources for its
proper use and safe.

Contents
This manual contains all the information necessary for the installation,
operation and maintenance of the instrument.
The scrupulous observance of what it is described guarantees a high degree
safety and productivity of the instrument.

2.2 KEEPING THE MANUAL

Keeping and consulting the manual


This manual is part of the instrument. It must be kept carefully and must be
always available for consultation, either by user both the installation and
maintenance workers.

Deterioration or loss
If you need to apply for another copy to the Distributor reference or
INTERLAB.
2. INTRODUCTION 13
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2.3 GUIDE TO READING

In this manual the points of great importance are highlighted symbols below.
Traders are advised to consult the important installation instructions and
operating instructions contained in this document and especially to observe
directions, regulations and prohibitions identified the following symbols:

WARNING

With this symbol is expressed significant memorandum practice for the


prevention of injuries or property damage.
The failure to follow marked with this symbol during the operation,
maintenance, repair or handling of the instrument can cause serious injury or
even death.

CAUTION

With this symbol is expressed a note of particular importance.


The failure to follow marked with this symbol during the operation,
maintenance, repair or handling of the instrument can cause minor injuries,
equipment damage, or loss important data.

NOTE

With this symbol is expressed as a note indicating the optimal use of the
facility are important reminders.

Users Manual Ver. 2.0


3. THE INTERLAB G26 INSTRUMENT

The INTERLAB G26 is a diagnostic medical device in vitro for electrophoretic


analysis of serum proteins, of unconcentrated urine proteins, of concentrated urine
proteins, of hemoglobin, of lipoproteins, of isoenzymes and for the execution of
several methods of immunofixation and isoelectric focusing.

3.1 OPERATION PRINCIPLE

The G26 INTERLAB by the separation of different protein fractions using the
principle of electrophoresis, that the difference in mobility between electrically
charged molecules that orient and migrate with different rates when subjected
to an electric field. The migration is performed at constant temperature,
obtained through the use of Peltier effect modules on agarose gel plates
containing a specific buffer solution according to the method to execute. The
gel acts as a support and molecular sieve and allows the different fractions
could be resolved on the basis of net charge possessed. After electrophoresis,
the gel is dried at a prescribed temperature, developed by appropriate
staining, destained and dried. The qualitative and/or quantitative colorimetric
detection occurs through the migration of the bands of fronts. For methods that
also allow the quantitative assessment, the measurement method is based on
optical densitometry reading of a source diode LED and a photodiode
detector. The signal obtained is sent to the PC to be processed. The
management of all phases of the analytical procedure is done through a
software system firmware factory installed on the instrument and a software
user interface installed on a PC that also returns the results of the analysis
and allows the processing and storage.

3.2 CHARACTERISTICS INTERLAB G26

The INTERLAB G26 is a new-generation device that allows, for the majority of
analytical methods, to run in full automation, the various stages of clinical
electrophoresis on agarose gel, minimizing operator intervention. The system,
composed of the instrument for analysis and related software
ELFOLAB / INTERLAB G26 installed on Personal Computer, allows to perform
full automation in the various stages of the analytical procedure.
3. THE INTERLAB G26 INSTRUMENT 15
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There are two versions of the instrument:

INTERLAB G26 without the sampler that runs the following steps:
dispensation of samples on agarose gel;
electrophoretic migration;
drying the gel;
gel staining;
gel destaining;
gel drying;
densitometric reading of the gel;
acquisition and data processing.

INTERLAB G26 with the sampler that runs the following steps:
identification of the tubes by reading a bar code;
sampling of samples from tubes;
deposit of samples in the sample plate;
dispensation of samples on agarose gel;
electrophoretic migration;
drying the gel;
gel staining;
gel destaining;
gel drying;
densitometric reading of the gel;
acquisition and data processing.

The system has great flexibility, allows a rational and efficient management of
time for analysis, with significant increase in speed of analysis and the ability
of the machine, thanks to:
- High degree automation;
- The wide variety of programs you can use;
- The various options you can use;
- The ability to schedule work sessions for the development of methods in
order of priority that can be changed if necessary;
- The possibility of processing methods in parallel, taking advantage of
downtime from one phase to the next.

With the INTERLAB G26, you can execute with complete automation, the
following analysis:
Protein electrophoresis of serum and of concentrated urines;
1-2 protein electrophoresis of serum and of concentrated urines;
Protein expanded electrophoresis of serum and of concentrated urines;
1-2 protein expanded electrophoresis of serum and of concentrated
urines;
Electrophoresis of the proteins in High Resolution;
Electrophoresis of the concentrated proteins in High Resolution;

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16 3. THE INTERLAB G26 INSTRUMENT
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Electrophoresis of the alkaline hemoglobins;


Electrophoresis of the acid hemoglobins;
Electrophoresis of the lipoproteins.

You can execute, with semiautomatic procedure, the following analysis:


Immunofixation of serum and of concentrated urines in Acid Blue;
Immunofixation of serum and of concentrated urines in Acid Violet;
Bence-Jones immunofixation of unconcentrated urines;
Bence-Jones Immunofixation of concentrated urines;
Pentavalent immunofixation of serum and of concentrated urines;
Electrophoresis of the lactate dehydrogenase isoenzymes (LDH);
Electrophoresis of the creatine kinase isoenzymes (CK);
Electrophoresis of the alkaline phosphatase isoenzymes (ALP);
Cryoglobulins;
CSF isoelectric focusing;
SDS proteinurie;
Electrophoresis of the -Amylase isoenzymes;
1-Antitrypsin isoelectric focusing;

NOTE
All methods perform with the instrument are described individually in
detail the procedures manual.

Users Manual Ver. 2.0


3. THE INTERLAB G26 INSTRUMENT 17
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3.3 TECHNICAL FEATURE

Power supply 85-265 V c.a. 47-63 Hz

Max Power consumption 250 VA

Interface to PC RS232

Principle Electrophoretic migration

Througt modules
Temperature control during the migration
Peltier effect

Scan path length 80 mm

Light source LED 623 nm

Optical detector Photodiode

Type of tube used Exterior diameter 10-16 mm


(only instrument with sampler) Height 100 mm

Reading bar codes


While entering the rack
(only instrument with sampler)

Time required for reading the 13 codes


Min 5 sec.
(only instrument with sampler)

UPC / EAN,
UPC / EAN Supplemental,
UCC / EAN 128, Code 39,
Code 39 Full ASCII,
TriOptic Code 39,
Types of bar codes read by the instrument Code 128,
(only instrument with sampler) Code128 Full ASCII,
Codabar, Code 2 of 5,
Codice 2 of 5 Interleaved,
Code 93, MSI, Code 11,
IATA, RSS Variants,
Chinese Code 2 of 5

Charge mode reagent Automatic

Storage time of samples dispensed >1h

Dimensions (L x W x H) 850 x 500 x 530 mm

Weight 45 kg

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18 3. THE INTERLAB G26 INSTRUMENT
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Temperature:
18C to +28C
Analysis conditions
Relative Humidity:
5% to 85% not condensing

Temperature:
0C to +40C
Operating conditions
Relative Humidity:
5% to 85% not condensing

Temperature:
da 0C a +50C
Storage conditions
Relative Humidity:
5% to 85% not condensing

Noise level 52 dB

Average thermal power dissipated 70 W

Ricycling

This product is indicated by selective collection for electrical


disposal and electronic equipment under the WEEE (Waste
Electrical and Electronic Equipment). This symbol indicates that
the product must be recycled or disposed in the implementation
of European Directive 2002/96/EC in order to minimize its impact
on the environment.
Electronic products are not subject to selective harvesting are
potentially hazardous to the environment and human health due
the presence of harmful substances.

Users Manual Ver. 2.0


3. THE INTERLAB G26 INSTRUMENT 19
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3.4 DESCRIPTION

3.4.1 OUTSIDE

Cover

Hood

Holes for the handles

Identification plate

Switch ON/OFF

Plug power supply

Serial port

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20 3. THE INTERLAB G26 INSTRUMENT
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Sample racks compartment

Distilled water tank pipe connection

Washing solution tank pipe connection

Destain solution tank pipe connection

Waste tank pipe connection

Biological waste tank pipe connection

Jack plugs

Users Manual Ver. 2.0


3. THE INTERLAB G26 INSTRUMENT 21
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3.4.2 INSIDE

Mechanical arm
Driven by system software, couplet by means an eletromagnet and moves
during the various stages of the analytical procedure: the needle dispenser,
applicators, the holder of the agarose gel, the lids of the tanks of the dyes.

Needle dispenser
Take samples from tubes housed in racks and rack dispenses them in
individual wells of the plate sample holder.

Site diluent container


Here it is placed the 2 ml container of the diluent for immunofixation.

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22 3. THE INTERLAB G26 INSTRUMENT
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Place of the needle wash


After each sampling, the needle is washed repeatedly with distilled water so
that you do not have a contamination between samples.

Wash applicator sites


After each application of the gel, the applicators are repeatedly washed with
the distilled water and make sure that there is no contamination between the
previous and the following samples.

Parking applicators
This is the site of the applicators.

Parking stain solution tank cover 1 (blue)


It is the place where the cover of the tank is used during the staining of the
stain solution 1.

Place of the decolouring treatment


In this site the gel holder undergo the phase of decolouring. It is also used to
wash the gel for immunofixation methods.

Parking stain solution tank cover 2 (violet)


It is the place where the cover of the tank is used during the staining of the
stain solution 2.

Oven
Here we begin the process of drying the gel at a temperature between 70 and
80 C using hot air.

Parking dye tank cover 3 (black)


It is the place where the cover of the tank is used during the staining of the
stain solution 3.

Optical drive
Through a diode LED and a photodiode, the readings at the end gel
densitometry of the whole analytical procedure. The relevant data collected
will be transferred to a PC for processing.

Parking gel
It is the place of the two frames where gel holder is taken at the beginning of
the analysis and deposited at the end.

Site of the three stain solution tanks


this is the place where the tanks 1, 2 and 3 are located.

Users Manual Ver. 2.0


3. THE INTERLAB G26 INSTRUMENT 23
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Site of the migration chamber


Through the electrical contacts there is the flow of current during the
electrophoretic migration.

Plate migration
The plate agarose gel adheres on it by creating the vacuum and the following
three phases:
dispensing of samples on the gel through the applicators;
electrophoretic migration through an electric field;
drying of the gel.
Use the Peltier effect to keep temperature constant during any phase of
electrophoretic migration and drying.

Site of the sample holder support


Before the analysis you insert the sample plate support.

Site of the racks and rack rails


This is the site of racks containing the tubes to allow the reading of the ID and
sampling.

Plate sample windows


After each sampling carried out from the needle dispenser the window is
closed to prevent the evaporation of the samples.

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24 3. THE INTERLAB G26 INSTRUMENT
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3.4.3 ACCESSORIES AND INTERNAL PLACEMENT

Applicators
Through the three applications 13 samples are taken from the plate sample
one by one and are applied on the gel. After each application, the applicator is
decontaminated by washing in the place.

Gel holder 1 and 2


They are a support of the gel plates during all the stages of the analysis. They
allow us to work in parallel, the instrument can process two gel plates
simultaneously.

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3. THE INTERLAB G26 INSTRUMENT 25
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Tanks stain dyes 1, 2, 3, and their covers


They contain three distinct colors: blue, violet and black for the operation of
coloring after drying the gel.

Migration chamber
Here there are the slots with the platinum electrodes where to place the
sponges soaked in electrolyte solution in order to conduct the current in the
gel. After the sampling on the gel, the migration plate rotates 180 degrees
clockwise by closing the migration chamber where the vacuum will be created.
The conductive gel plate will adhere to the sponges and the gel will be
subjected to the potential difference that will cause the electric field for
electrophoresis.

Plate sample support


It is laid the place disposable sample with 39 holes.

Racks 1, 2, 3
It consists of 3 rows of 13 holes each in which there are the tubes with the
samples. Through a bar code printed on each tube, the system is able to read
the patient information and the type of analysis to read and transmit data to
your PC.

Users Manual Ver. 2.0


4. INSTALLATION

4.1 OPERATING ENVIRONMENT

To ensure optimum operation of the instrument and the reliability of the results
follow the instructions below.

4.1.1 ENVIRONMENTAL REQUIREMENTS


Observe the operating conditions required by technical features in
section 3.3, especially the temperature and the relative humidity of
the environment in which the instrument is installed.
Install the instrument in a room that meets the requirements for
laboratory safety.

CAUTION

 Do not expose the instrument to the sunlight, heat sources and


in a dusty environment.
 Keep a working environment clean and tidy.

4.1.2 MINIMUM CONFIGURATION SYSTEM


For a proper installation and an optimum operation of the software
Elfolab/Interlab G26, the personal computer requires the following
minimum requirements:
Processor Pentium or Celeron class;
256 MB RAM;
Graphics card with 64k colors;
Screen resolution 1024 x 768 pixels;
100 MB free Hard-Disk,
Operating System Windows XP, Windows Vista, Windows 7;
Serial port RS232;
USB port.
4. INSTALLATION 27
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4.2 UNPACKING AND CHECKING

Follow the instructions below for unpacking the instrument. Inspect carefully
the instrument and accessories in the box enclosed within the same
instrument. In case of damages found or accessories are missing, contact the
Reference Distributor or INTERLAB.

4.2.1 UNPACKING INSTRUCTIONS

Remove the four L-shaped metal fasteners that hold the outer casing
of cardboard to the wooden base. Use a screwdriver inserted
between the stopper and the base metal and force it downwards until
the single firm has dropped.
Then lift up the casing of cardboard to remove it, as indicated by four
arrows in the figure.

NOTE
Keep the original packaging for a possible transport of the
instrument.

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28 4. INSTALLATION
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Remove the allen key on the right side of the instrument.


With the help of the key unscrew by turning counterclockwise, the
four bolts holding the two auction block at the base of the instrument
package.

Pull in the direction indicated by arrows the two auction block to free
the instrument from the base of the packaging.
Place the instrument in the installation site and remove it from
protection.

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4. INSTALLATION 29
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Open the instrument by raising the transparent cover, making it


perform a rotational movement upwards.
Remove carefully the box from the instrument containing the
accessories, open it and check the contents.

CAUTION

 For any handling of the package containing the instrument


use a fork lift.
 Any handling operation of the instrument must be done in
two.
 Keep the original packaging for any possible transport
instrument.

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30 4. INSTALLATION
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4.2.2 CHECK ACCESSORY BOX CONTENTS


The box must contain the accessories described in the enclosed model.

Code Description N. Picture

SAE909M Applicators 3

SAE911M Chamber migration 1

Racks 3

Tank
SAE912M STAIN 1
SOLUTION 1

Tank
SAE913M STAIN 1
SOLUTION 2

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4. INSTALLATION 31
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Tank
SAE914M STAIN 1
SOLUTION 3

Covers of the stain


3
solution tanks

Whole tank
SAE915M WASHING 1
SOLUTION

Whole tank
DISTILLED 1
WATER

Whole tank
SAE916M DESTAIN 1
SOLUTION

Diluent container
1
for 2 ml

Probe for needle 1

Waste tank cap 2

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32 4. INSTALLATION
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Pipe 5x10 to waste


1
tank

SAE917M Waste tank label 1

Pipe 3x6 to biological


waste tank with 1
hydraulic connection

Biological waste tank


1
label

SAE401M RS232 cable 1

SAE548M Power cable 1

SAE919M Holders 2

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4. INSTALLATION 33
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Support sample
SAE920M 1
holder

Grid lock for plate


SAE800M samples 1

SAE140M Mousepad 1

Handles 4

CONTENTS OF THE ENCLOSED ENVELOPE


CD ROM Software
SAE780M 1
and manuals
Scanner mask 1
P022 Safety Test Report 1
Declaration
1
conformity CE
P043 List accessories form 1

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34 4. INSTALLATION
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4.3 INSTRUMENT INSTALLATION

To ensure optimum operation of the instrument, the installation should be


performed by technical staff from INTERLAB.

4.3.1. LOCATION
Place the instrument on a flat horizontal surface of minimum size
150x70 cm, clean, stable and vibration-free, at a height of about 85
cm above the floor so the operator can perform the normal operating
procedures easily.
Check the horizontality of the instrument through a spirit level.
Check around the instrument there is a minimum clearance of 15 cm
to allow the air circulation for cooling.
Place the instrument to make easier the connection and
disconnection operations of external accessories (solution tanks and
waste tanks) and electrical connections.

WARNING

 Do not install the instrument near the sinks, possible water


jets and containers liquids that can leak out. The liquids
spilled on the instrument can cause serious damages.
 Do not block ventilation holes of the instrument to ensure
adequate air circulation.

4.3.2. EXTERNAL ACCESSORY ASSEMBLY

Connect the two pipes of the distilled water tank to the hydraulic
connections marked by the label DISTILLED WATER. Connect the
jack to level control at one of the three jack plugs.
Connect the pipe of the washing solution tank to the hydraulic
connection marked by the label WASHING.
Connect the pipe of the destain solution tank to the hydraulic
connection marked by the label DESTAIN SOLUTION.

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4. INSTALLATION 35
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Connect the waste tank pipe to the hydraulic connection marked by


the label WASTE. Connect the jack to level control at one of the
three jack plugs.
Connect biological waste tank pipe to the hydraulic connection
marked by the label WASTE. Connect the jack to level control at
one of the three jack plugs.

CAUTION

 The tanks (distilled water, washing solution, destain solution)


must be placed on the same plane as the instrument.
 The waste and the biological waste tanks must be placed on
the floor or under the level of the instrument.

4.3.3. PC CONNECTION
Connect by RS232 cable supplied with the computer's serial port to
the serial port of the instrument on the right side.

4.3.4. ELECTRICITY GRID CONNECTION


Make sure the voltage and the frequency of the electricity grid in the
country where the instrument is installed correspond to the features
on the identification plate and in this manual.
Make sure the switch on is in position 0 (off).
Connect using the supplied power cable into the electricity grid.
If you must use an extension cable to connect the instrument to the
electricity grid, make sure it has been approved and equipped with
protective conductor (ground).

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36 4. INSTALLATION
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WARNINGS

 The instrument should be linked only to a wiring with an


efficient ground and complying with the legislation of the
country.
 Any interruption protective conductor inside or outside the
instrument, or the inadequate performance of the ground can
make the instrument dangerous.

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4. INSTALLATION 37
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4.4 ELFOLAB / INTERLAB G26 SOFTWARE INSTALLATION

4.4.1. PRELIMINARY OPERATIONS FOR USERS WINDOWS VISTA

Users running Windows Vista before installing the software


Elfolab/Interlab G26, remove some protections that prevent the proper
program functions.

Removing of the Windows Defender

1. Enter the Control Panel from the Start Menu low in the left

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38 4. INSTALLATION
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2. Select the icon Windows Defender.

3. Select icon Tools.

4. Select icon Options.

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4. INSTALLATION 39
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5. Remove all the "Options".

6. Save your changes.

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40 4. INSTALLATION
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Removing User Account Control

1. Enter the Control Panel from the Start Menu low in the left .

2. Select the icon User Account.

3. Select the icon Enable or Disable the User Account Control.

4. Disable the Control Account and click OK.

5. Restart your PC.

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4. INSTALLATION 41
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4.4.2. PRELIMINARY OPERATIONS FOR USERS WINDOWS 7

Users running Windows 7 before installing the software Elfolab / Interlab


G26, remove some protections that prevent the proper program
functions.

Removing User Account Control

1. Enter the Control Panel from the Start Menu low in the left.

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42 4. INSTALLATION
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2. Select System and security.

3. Select Modify the settings of User Account Control.

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4. INSTALLATION 43
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4. Lower the bar across the bottom.

5. Click OK.

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44 4. INSTALLATION
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4.4.3. ELFOLAB PROGRAM INSTALLATION

NOTE
Before reainstalling or installing an update, you must follow a
procedure to uninstall the previous version before loading
again Elfolab or Interlab G26 on your computer.

Installation procedure

1. Insert the CD INTERLAB CD in the CD/DVD drive on your PC.

2. Select the icon Open folder to view files using Windows


Explorer and click OK.

3. Select the folder Softwares.

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4. INSTALLATION 45
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4. Select the folder Elfolab x.x.x. inside the CD.

5. Select Setup.exe icon.

6. Close all the applications and click OK.

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46 4. INSTALLATION
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7. Choose the installation directory (already set) and press the button
with the icon PC.

8. Click Continue.

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4. INSTALLATION 47
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4.4.4. INTERLAB G26 APPLICATION INSTALLATION

1. Select the Software->INTERLAB G26 directory from the CD-Rom.


2. Double-click the Setup file and press Open.
3. In the opening window, click OK.

4. Click the button with the icon PC to start the installation.

5. Click Continue.

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48 4. INSTALLATION
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6. If this dialogue window appears, you should always answer Yes.

7. Click OK.

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4. INSTALLATION 49
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4.4.5. ELFOLAB AND INTERLAB G26 UNINSTALLATION

Uninstallation for users Windows XP or Vista

1. From the desktop click Start on the taskbar.


2. Select the item in the Control Panel.
3. In the Control Panel, double-click Add/Remove Programs.
4. Add/Remove Programs dialog window, select from the list of
InterlabG26 Elfolab or currently installed, and then click on
Add/Remove button.
5. Answer Yes when asked to confirm the removal of Elfolab or
InterlabG26 by computer.
6. If a dialogue window appears asking whether or not to keep the
shared files. You should choose Remove All.
7. Windows XP / Vista will automatically delete the application files
from Hard-Disk and remove references from the application log of
Windows, afterwards the message confirming uninstallation.
8. If you click OK will close the operation.

Uninstallation for users Windows 7

1. From the desktop click the Start button on the taskbar.


2. Select the item in the Control Panel.
3. In the Control Panel, click Uninstall a sightseeing program.
4. Select Elfolab InterlabG26 or from the list of currently installed with
a double click.
5. Answer Yes when asked to confirm the removal of Elfolab or
InterlabG26 by computer.

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50 4. INSTALLATION
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6. If a dialogue window appears asking whether or not to keep the


shared files. You should choose Remove All.
7. Windows XP / Vista will automatically delete the application files
from Hard-Disk and remove references from the application log of
Windows, afterwards the message confirming uninstallation.

NOTE
Uninstalling Elfolab and/or Interlab G26 does not delete the
database work.

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4. INSTALLATION 51
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4.5 INSTRUCTIONS FOR TRANSPORT AND STORAGE

4.5.1. INSTRUCTIONS FOR EACH MOVEMENT


Remove from the instrument the tanks of stain solutions together with
covers, empty and wash them with running water and finally rinse
them with distilled water.
Remove the applicators.
Remove the holders.
Close the cover.
Disconnect from the instrument all the connecting pipes to the tanks
including the level control jacks.
Disconnect the instrument from the electrical grid and unplug the
power cable.
Disconnect the cable from the PC.
Screw the threaded holes till the bottom on either side of the
instrument, the four handles for the movement.
Perform any movement of the instrument by grasping the handles.

WARNINGS

 During the use of the instrument and handling its internal and
exterior accessories be careful to wear all personal protective
equipment (gloves, goggles, laboratory gown and surgical)
 Pay attention before disconnecting the biological waste and
its accessories.

CAUTION

 Any handling operation of the instrument must be done in


two.
 Make sure you have enough space to move the instrument.

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52 4. INSTALLATION
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4.5.2. INSTRUCTION FOR TRANSPORT


Remove all internal accessories from the instrument, empty the stain
tanks , wash them thoroughly with running water, rinse them with the
distilled water, dry them, wrap them with the plastic wrap and store it
in a box.
Disconnect from the instrument all the external accessories.
Disconnect the instrument from the electrical grid and unplug the
power cable.
Disconnect the cable to the PC.
Empty the tanks of distilled water, washing solution and destain
solution.
Dispose of the waste liquids as provided by the present law.
If it is necessary clean the inside of the instrument.
Lock the mechanical arm and the dispenser needle by using
stratocell blocks and tape to prevent them from moving during the
transport.
Pack the instrument by using its original packaging by performing
inversely unpacking described in section 4.2.1. During the packaging
operation keep the directions of the indicators printed up on the box.
For any handling of the package containing the instrument use a fork
lift.

4.5.3. INSTRUCTIONS FOR STORAGE


Perform all the operations described in section 4.4.2..
Place the instrument and its accessories packed in a clean and dry at
a temperature between 0 C and 50 C.
Put the instrument in a place where it can not be subjected to
accidental impact.

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CAUTION

 Do not turn over the box with the instrument packed.


 Do not bring the box with the instrument to rain, to high humidity or low
temperatures or very high.
 Pay attention to the symbols and indicators printed on the box.
 Make sure you have enough space to fulfill all handling operations easily.
 The condensation inside the instrument when it is subjected to very low
temperatures and/or high humidity can cause serious damages to
electronic parts.
 If the instrument has been subjected to a temperature below 5 C for more
than 24 hours as a precaution, leave it to wait for at least 4 hours in an
environmental temperature of about 25 C.

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5. STARTING

5.1 THE STARTING OF THE INSTRUMENT

The procedures described below are common to all methods they should be
carried out completely the first time when the instrument is in use. Some
procedures must be chosen according to the methods that you intend to run
and repeat as needed.

5.1.1 THE FILLING OF THE EXTERNAL TANKS


This procedure should be performed for any method and repeated
whenever it runs out of a liquid.
To make the filling operations of the external tanks it is not necessary to
disconnect the connecting pipes from the instrument.

Filling the tank of distilled water


Unscrew by turning counterclockwise, the green ring nut of cap.
Remove the cap with the level sensor and suction pipes of liquid
inside the tank.
Fill the tank with distilled water.
5. STARTING 55
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Replace the cap clockwise and screw the green ring nut.

Filling the tank of washing solution


Unscrew by turning counterclockwise, the green ring nut of cap.
Remove the cap with the level sensor and suction pipe of liquid
inside the tank.
Fill the tank with the washing solution to the maximum level showed
by the indicator in the picture.
Replace the cap and screw clockwise the green ring nut.

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56 5. STARTING
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Filling the tank of destain solution


Unscrew by turning counterclockwise, the black ring nut of the cap.
Remove the cap with the suction pipe of the liquid inside the tank.
Fill the tank with the destain solution.
Replace the cap and screw clockwise the black ring nut.

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5.1.2 PREPARATION OF THE STAIN TANKS

Fill the tanks respectively with the three solutions:


STAIN SOLUTION 1, STAIN SOLUTION 2, STAIN SOLUTION 3
up to a level between the two MIN and MAX marks as showed by the
indicators in the figure.
Close the tank with the covers.

Use the STAIN SOLUTION 1 to perform the following methods:


 Proteins electrophoresis of the serum and of the urines;
 Electrophoresis of the Hemoglobins;
 Immunofixation of the serum and of the concentrated urines in
Blue Acid.
IMPORTANT: Replace the stain solution of the tank 1 after five rounds
of staining (5 treated gel plates).

Use the STAIN SOLUTION 2 to perform the following methods:


 Proteins electrophoresis in high resolution;
 Immunofixation of the serum and of the concentrated urines in
Violet;
 Pentavalent immunofixation of the serum and of the concentrated
urines;
 Bence-Jones immunofixation of the serum and of the concentrated
urines;
 SDS proteinurie of the unconcentrated urines.

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58 5. STARTING
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IMPORTANT: Replace the stain solution of the tank 2 after five rounds
of staining (5 treated gel plates).

Use the STAIN SOLUTION 3 to perform the following methods:


 Lipoprotein electrophoresis;
 Electrophoresis of the Alkaline Phosphatase Isoenzymes;
 Electrophoresis of the Lactate Dehydrogenase Isoenzymes;
 Electrophoresis of the CK isoenzymes.
IMPORTANT: After staining replace the stain solution of the tank 3 (one
treated gel plate).

It is not necessary to fill all the three tanks if you do not have to follow
all the methods above mentioned. You can also use the instrument only
with one or two tanks necessary to perform the methods.

Insert the three tanks of the dye solutions 1, 2 and 3 respectively in


the three spaces marked with the labels
Perform the movements showed by the indicators in the figure.
To remove the tanks perform the movement showed by the
indicators in the sequence and in the opposite direction.

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5. STARTING 59
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5.1.3 POSITIONING OF THE APPLICATORS

Insert gently the 3 applicators in the designated parking marked on


the label by grasping the upper extremities.

IMPORTANT: At the bottom of the applicators, there are dispensing


blades of the samples on the gel, they are very delicate. Do not touch
the jagged applicators and not put them on the bottom when they are
out of their parks to avoid damaging them.

WARNINGS

 The blade applicators are sharp. Do not touch them with your
fingers to avoid injury.
 The applicators come into contact with potentially infectious
samples: biological hazards.

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60 5. STARTING
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5.1.4 PREPARATION OF THE SAMPLE PLATE SUPPORT


This procedure should be performed for all methods.

Withdraw from the kit of the method to run the sample plate
disposable B.
Prepare the sample holder support by placing in the site C the
sample plate B by means of the grid and lock mounting as showed in
the figure on the left.
Insert the prepared sample plate support in the site on the left side of
the instrument, pushing it to the end.

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5. STARTING 61
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5.1.5 PREPARATION OF THE MIGRATION CHAMBER


This procedure should be performed for all methods.

Withdraw from the kit of method to run the sponges (2 or 3) already


soaked in electrolyte solution.
Set the sponges with tweezers in the rectangular slots of the
migration chamber. Do not push them with too much pressure.
Make sure the sponges are correctly inserted and they are reaching
the bottom of their entire length.

IMPORTANT: Take the sponges soaked in electrolyte solution with


clean tweezers and clean gloves.

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62 5. STARTING
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Insert the migration chamber in the site located at the center of the
instrument, slightly tilted forward.
Push it forward until it stops, then, down slightly until you hear a click
indicating the correct position.

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5. STARTING 63
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5.1.6 PREPARATION OF THE GEL


This procedure should be performed for all methods.

Withdraw from the kit of the method to run the agarose gel plate and
a blotting paper. The plates are disposable and ready for use.
Place the plate on a clean sheet of paper turning up on the top with
the gel. The top enables the reading of silk screen printed on the gel
plate.

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64 5. STARTING
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Run through the blotting paper the absorb excess of the buffer
solution, making sure that the blotting paper adheres to the entire
surface of the plate.

Take the gel plate, holding the palm of your hand from both ends,
remove the blotting paper quickly.

IMPORTANT: Do not leave for too much time the blotting paper on the
gel to avoid its excessive dehydration.

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5. STARTING 65
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Insert the gel plate gently in the holder, slide it into the appropriate
guide rails. To advance it is necessary to impress a slight upward
curve and apply a little pressure forward.
Finally check the gel plate is properly positioned between the rails of
the holder.

IMPORTANT: The gel plate should be oriented with the printed


indicators pointing to the top of the holder.

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66 5. STARTING
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Insert the holders 1 and/or 2 in the numbered parkings located on the


right side of the instrument.
Close the cover of the instrument.

IMPORTANT: Follow the numbering of the holders and the relating


parkings.

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5.1.7 PREPARATION OF THE RACKS


This procedure is performed only for the instrument with the sampler.

Insert the sample tubes into the holes of the rack turning toward the
opening of each hole in order to read the bar code label of the tubes.

IMPORTANT: The tubes should contain a volume of the sample liquid


at least 300 L.

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68 5. STARTING
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Place the racks according to the relation between the number of the
rack and the site. Do not try to reverse the position of the racks.
Place the racks at a constant speed and taking at least 5 seconds to
allow the reading of the bar codes on the tubes.
Push each rack in the bottom of the site until you hear the magnetic
block.

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5.2 PRELIMINARY CONTROLS BEFORE STARTING

Before starting each method follow the controls below:

Make sure that in the tanks of distilled water, washing solution and destain
solution there is a sufficient level of the liquid.
Check the fluid level in the tanks of waste and biological waste; replace
them if they are full.
Check the level of the stain solutions for the required methods through the
little window on the front of each tank. Check that any liquid level is
between the two notches MIN and MAX.
Check the three applications are properly inserted in their parking.
Make sure the dispenser needle is located correctly in its parking.
Check the sample plate support is properly inserted and locked.
Check the migration chamber is properly prepared, inserted and locked.
Make sure the racks have been properly prepared by placing the tubes in
order to allow the reading of the bar codes. Make sure each rack is
inserted and locked.

Users Manual Ver. 2.0


6. THE ELFOLAB / INTERLAB G26 SOFTWARE

6.1 THE ELFOLAB PROGRAM

6.1.1 ELFOLAB STARTING


1. Click the Start button on the taskbar.
2. If you use Windows XP or Vista, select Programs .

3. Click on the icon .


4. The sofware starts showing the following screen:
6. THE ELFOLAB / INTERLAB G26 SOFTWARE 71
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6.1.2 DATABASE OPENING


The database is a list of the information on patients. The database is
divided into the historical part and the daily part. The data sent from the
instrument are first received in the daily session, after you can record
them permanently and enrich its historical part.

The database is initially empty, to open it and enter data for new
patients or working on the day or the historical part, follow these
instructions:
1. Click on the Database and select from the men the Open
Database:

2. When the screen is open, click on the icon or open the


Database menu and select Working Database.

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72 6. THE ELFOLAB / INTERLAB G26 SOFTWARE
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3. when windows opens

click on the button:

a) to enter new patients; the Select


Methods window will open automatically

b) to retrieve the data from recent session


and/or open the Select Methods window.

c) to open the Historical Database.

4. Select the method or the methods to perform, each of them


corresponds to a session:

and click on the button to confirm or on the button to

delay this step later, if so, just click on the button or click Select
Methods to reopen this window.

Complete the selection of the methods with the button the


panel of the Interlab G26 application will open with the sessions set
up before thanks to them it is possible to start the analysis (See
6.2.2).

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6. THE ELFOLAB / INTERLAB G26 SOFTWARE 73
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5. To enter a patient data click on the box of


the patient record created and insert the patient's ID number.
Entering the other boxes to add further data.
6. To insert other data of patients it is sufficient to press the buttom
Enter/Return on your keyboard.

NOTE
Database: Each patient has a record with the identification
data, the graphs and the values of the analysis made and all
the notes.

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74 6. THE ELFOLAB / INTERLAB G26 SOFTWARE
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6.2 THE INTERLAB G26 APPLICATION

6.2.1 INTERLAB G26 STARTING


To start the Interlab G26 application:
Open from Elfolab a New Working Database or the Current Working
Database and choose the methods to be performed (See 6.1.2). The
sessions created by Elfolab will be sent automatically Interlab G26
application.

1. Click on the Run INTERLAB G26 or on the button .


2. You will see the main panel of the application.

6.2.2 THE MAIN PANEL

The main panel shows the application of the method sessions that have
been previously selected (See 6.1.2), the parameters of the methods
to start the working and a field for the choice of options. Before
restarting the methodology you must select the following options.
1. Choose from the options the method (only the instrument with the
sampler):
Sampling + Analysis For reading the bar codes, for
sampling and for starting the
analysis.

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Sampling For reading the bar codes and for


sampling.
Analysis For starting the analysis immediately.
Scanning For reading the bar codes.
2. Choose the holder number related to the session method to be
started:
Gel holder 1 If the gel of the session method has
been prepared on the holder 1.
Gel holder 2 If the gel of the session method has
been prepared on the holder 2.
3. Start by clicking on the relating session.
4. In the confirmation window click on the button Yes to confirm or on
the button No to go back.

6.2.3 BAR CODE READING


(Only the instrument with the sampler)

The system can read the bar codes from the tubes, retransmit the ID to
the database of patients and perform the sampling.
If you choice one of the three options: Sampling+Analysis, Sampling
or Scanning by the main panel of Elfolab G26 (See 6.2.2) then on the
session starting, it opens the window for reading the bar codes. In each
field the ID code, read by the tubes, will be displayed The number of the
fields displayed depends on the chosen method.

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76 6. THE ELFOLAB / INTERLAB G26 SOFTWARE
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When the window opens, all the fields will be selected by default; in this
case after inserting the racks a green light will turn on for each bar code
read correctly.

You can also get only the ID of some tubes; in this case deselect the
fields

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The incorrect reading of the ID

At the incorrect reading of the ID a red light will turn on.

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78 6. THE ELFOLAB / INTERLAB G26 SOFTWARE
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The causes can be:


a) An incorrect insertion of the tubes in the rack;
b) A quick insertion of the rack in the site;
c) An unreadable bar code.
You can work successfully by repositioning the unreadadble tubes and
by inserting the rack more slowly or by entering the missing ID into the
field directly.

ID insertion manually

To insert an ID manually or to change an ID already read first click on


the button Change Id Manually and then go to the box and write the
new ID.

Duplicated ID

If one or more ID are equal, the box will become red and a dialogue
window will invite you to check the correct combination before starting
the sampling.

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Start sampling after the correct combination between the read ID and
the printed ID on the tubes, by clicking No or Yes to check it.

The insufficient liquid in the tubes

When the volume of the liquid in the tube is less than 300 L the
following window appears:

Click on the button OK after checking the volume of the liquid.

The starting of the analysis by using the only dispensed samples

It is possible to start the analysis by using the only dispensed samples


when ID is unreadable or after an unsuccessful sampling.

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80 6. THE ELFOLAB / INTERLAB G26 SOFTWARE
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To start the analysis click on the button Start Analysis with only
dispensed samples.

NOTE
After reading the bar code you have only just 3 minutes to
start the sampling. Otherwise the window will close without
sampling.

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6.3 THE INTERLAB G26 MENU

6.3.1 SETTINGS
Generals

It sets and saves the general settings:


 Communication port PC/instrument interface;
 How to start the analysis.

Diluitions

The dilution ratios for the IFE methods and ALP isoenzymes
electrophoresis are by default. They can be modified and saved for any
use through the following window:

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82 6. THE ELFOLAB / INTERLAB G26 SOFTWARE
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6.3.2 LANGUAGE
It is possible to set the language from the list.

6.3.3 SETTING PARAMETERS


It allows you the variation of parameters of the methods. This item has
a password for the protection.

CAUTION

The parameters of the methods are set by default to optimize the


instrument operation and reliability of the analysis results. Upon
request, INTERLAB can release the password to change the
parameters. In this case INTERLAB does not guarantee the
optimal operation of the instrument, the reliability of the results
and it is not liable for any damages.

6.3.4 INTERLAB s.r.l.


Web Site

Link to Web Site: www.interlab-srl.com.

Contact us

It allows you to send an e-mail: info@interlab-srl.com.

Users Manual Ver. 2.0


7. METHODS OF ANALYSIS

7.1 PROTEIN ELECTROPHORESIS OF SERUM AND OF URINES

The instrument runs the following steps of the methods, in an automated way:
reading of the patients ID from tubes and sampling (only for instrument
with the sampler);
application of samples on the gel plate;
electrophoretic migration;
gel drying on the plate Peltier;
gel staining;
gel destaining;
gel drying;
densitometric reading.

7.1.1 PROTEIN ELECTROPHORESIS OF SERUM AND OF


CONCENTRATED URINES 5 FRACTIONS 13 TESTS
Make reference to the PK110 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 5-13 in the session to be set (See 6.1.2):
84 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

6. After selecting the session or sessions confirm by clicking on


the button .

7. Select the options of the analysis startup and begin the session
by clicking on the button SPE 5F.-13 (See 6.2.2).

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.
10. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

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7. METHODS OF THE ANALYSIS 85
_________________________________________________________________________________

7.1.2 PROTEINS ELECTROPHORESIS OF SERUM AND OF


CONCENTRATED URINES 5 FRACTIONS 26 TESTS
Make reference to the PK110 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 5-26 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button SPE 5F.-26 (See 6.2.2).

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86 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.
10. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.3 PROTEINS ELECTROPHORESIS OF SERUM AND OF


CONCENTRATED URINES 5 FRACTIONS 39 TESTS
Make reference to the PK110 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

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7. METHODS OF THE ANALYSIS 87
_________________________________________________________________________________

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 5-39 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button SPE 5F.-39 (See 6.2.2).

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.

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88 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

10. Remove the soaked sponges by migration chamber and


remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.4 1-2 PROTEIN ELECTROPHORESIS OF SERUM AND OF


CONCENTRATED URINES 6 FRACTIONS 13 TESTS
Make reference to the PK119 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support (See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 6-13 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button SPE 6F.-13 (See 6.2.2).

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7. METHODS OF THE ANALYSIS 89
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.
10. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.5 1-2 PROTEIN ELECTROPHORESIS OF SERUM AND OF


CONCENTRATED URINES 6 FRACTIONS 26 TESTS
Make reference to the PK119 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support (See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

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90 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 6-26 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button SPE 6F.-26 (See 6.2.2).

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.

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7. METHODS OF THE ANALYSIS 91
_________________________________________________________________________________

10. Remove the soaked sponges by migration chamber and


remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.6 1-2 PROTEIN ELECTROPHORESIS OF SERUM AND OF


CONCENTRATED URINES 6 FRACTIONS 39 TESTS
Make reference to the PK119 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support (See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 6-39 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button SPE 6F.-39 (See 6.2.2).

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92 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.
10. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.7 PROTEIN EXPANDED ELECTROPHORESIS OF SERUM AND OF


CONCENTRATED URINES 5 FRACTIONS 13 TESTS
Make reference to the PK110 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

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7. METHODS OF THE ANALYSIS 93
_________________________________________________________________________________

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 5-13 Expanded in the session to be set (See
6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button SPE 5F.-13Exp (See 6.2.2).

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.

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94 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

10. Remove the soaked sponges by migration chamber and


remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.8 PROTEIN EXPANDED ELECTROPHORESIS OF SERUM AND OF


CONCENTRATED URINES 5 FRACTIONS 26 TESTS
Make reference to the PK110 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 5-26 Expanded in the session to be set (See
6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button SPE 5F.-26Exp (See 6.2.2).

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7. METHODS OF THE ANALYSIS 95
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.
10. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.9 1-2 PROTEIN EXPANDED ELECTROPHORESIS OF SERUM AND


OF CONCENTRATED URINES 6 FRACTIONS 13 TESTS
Make reference to the PK119 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support (See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

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96 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 6-13 Expanded in the session to be set (See
6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button SPE 6F.-13Exp (See 6.2.2).

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.

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7. METHODS OF THE ANALYSIS 97
_________________________________________________________________________________

10. Remove the soaked sponges by migration chamber and


remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.10 1-2 PROTEIN EXPANDED ELECTROPHORESIS OF SERUM AND


CONCERNTRATED URINES 6 FRACTIONS 26 TESTS
Make reference to the PK119 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
Serumproteins 6-26 Expanded in the session to be set (See
6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button SPE 6F.-26Exp (See 6.2.2).

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98 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with sampler). (See
5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.
10. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.11 PROTEIN ELECTROPHORESIS IN HIGH RESOLUTION


Make reference to the PK118 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

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7. METHODS OF THE ANALYSIS 99
_________________________________________________________________________________

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
HR Proteins in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button H.R. Proteins (See 6.2.2).

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.

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100 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

10. Remove the soaked sponges by migration chamber and


remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

7.1.12 CONCENTRATED PROTEIN ELECTROPHORESIS IN HIGH


RESOLUTION
Make reference to the PK118 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
Proteins H.R. Concentrated in the session to be set (See
6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button H.R. Proteins Con (See 6.2.2).

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7. METHODS OF THE ANALYSIS 101
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.
10. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

Users Manual Ver. 2.0


102 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

7.2 IMMUNOFIXATION OF THE SERUM AND OF THE URINES (IFE)

The methods of the immunofixation performed by the instrument INTERLAB


G26 are made by semiautomatic procedures.

The automated steps are:


reading of the ID patients from the tubes and sampling (only the
instrument with the sampler);
application of the samples on the gel plate;
electrophoretic migration;
drying of the gel plate Peltier;
washing the gel;
further drying;
gel staining;
gel destaing;
gel drying;

7.2.1 IMMUNOFIXATION OF SERUM AND OF CONCENTRATED URINES


IN ACID BLUE 2 TESTS
Make reference to the PK128 procedure of the CD included in the kit.
I. Preparing of the analysis
1. Check the level of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE Acid Blue 2 in the session to be set (See 6.1.2):

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7. METHODS OF THE ANALYSIS 103
_________________________________________________________________________________

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE Acid Blue 2 (See 6.2.2).

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After the migration take the holder with the gel from the
instrument and run manually by using the Easy Mask for the
external steps: application of the fixative solution and the
antiserum, incubation of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click on the button Restart and wait until the end of the
analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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104 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

7.2.2 IMMUNOFIXATION OF SERUM AND OF CONCENTRATED URINES


IN ACID BLUE 4 TESTS
Make reference to the PK128 procedure of the CD included in the kit.
I. Preparing of the analysis
1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE Acid Blue 4 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE Acid Blue 4 (See 6.2.2).

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7. METHODS OF THE ANALYSIS 105
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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106 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

7.2.3 IMMUNOFIXATION OF SERUM AND OF CONCENTRATED URINES


IN ACID BLUE 6 TESTS
Make reference to the PK128 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE Acid Blue 6 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE Acid Blue 6 (See 6.2.2).

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7. METHODS OF THE ANALYSIS 107
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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108 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

7.2.4 IMMUNOFIXATION OF SERUM AND OF CONCENTRATED URINES


IN ACID VIOLET 2 TESTS
Make reference to the PK127 procedure of the CD included in the kit

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE Acid Violet 2 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE Acid Violet 2 (See 6.2.2).

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7. METHODS OF THE ANALYSIS 109
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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110 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

7.2.5 IMMUNOFIXATION OF SERUM AND OF CONCENTRATED URINES


IN ACID VIOLET 4 TESTS
Make reference to the PK127 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE Acid Violet 4 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE Acid Violet 4 (See 6.2.2).

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7. METHODS OF THE ANALYSIS 111
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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112 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

7.2.6 IMMUNOFIXATION OF SERUM AND OF CONCENTRATED URINES


IN ACID VIOLET 6 TESTS
Make reference to the PK127 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE Acid Violet 6 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE Acid Violet 6 (See 6.2.2).

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7. METHODS OF THE ANALYSIS 113
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.2.7 BENCE-JONES IMMUNOFIXATION OF THE UNCONCENTRATED


URINES 2 TESTS
Make reference to the PK126 procedure of the CD included in the kit.

I. Preparing the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE BJ 2 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE BJ 2 (See 6.2.2).

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7. METHODS OF THE ANALYSIS 115
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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.2.8 BENCE-JONES IMMUNOFIXATION OF THE UNCONCENTRATED


URINES 4 TESTS
Make reference to the PK126 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE BJ 4 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE BJ 4 (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.2.9 BENCE-JONES IMMUNOFIXATION OF THE UNCONCENTRATED


URINES 6 TESTS
Make reference to the PK126 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE BJ 6 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE BJ 4 (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.2.10 BENCE-JONES IMMUNOFIXATION OF THE CONCENTRATED


URINES 2 TESTS
Make reference to the PK126 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE BJ2 Concentrated in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE BJ2 Concentrated (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.2.11 BENCE-JONES IMMUNOFIXATION OF THE CONCENTRATED


URINES 4 TESTS
Make reference to the PK126 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE BJ4 Concentrated in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE BJ4 Concentrated (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.2.12 PENTAVALENT IMMUNOFIXATION OF SERUM AND OF


CONCENTRATED URINES 6 TESTS
Make reference to the PK133 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE Penta 6 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE Penta 6 (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.2.13 PENTAVALENT IMMUNOFIXATION OF SERUM AND OF


CONCENTRATED URINES 12 TESTS
Make reference to the PK133 procedure of the CD included in the kit.

I. Preparing of the analysis


1. Check the level of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting analysis


5. In the Select Methods Elfolab menu choose the method
IFE Penta 12 in the session to be set (See 6.1.2):

6. After setting the session or sessions confirm by clicking on the


button .

7. Select the startup options analysis and begin the session by


clicking on the button IFE Penta 12 (See 6.2.2).

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_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After migration take the holder with the gel from the instrument
and run manually using the Easy Mask for the external steps:
application of the fixative solution and the antiserum, incubation
of the gel, gel blotting.

10. Place the gel holder in its parking.

11. Click the Restart button and wait until the end of the analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by migration chamber and
remove and clean the slots with a paper dampened with
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.3 ELECTROPHORESIS OF THE HEMOGLOBINS

The instrument runs the following steps of the methods, in an automated way:

reading of the patients ID from tubes and sampling (only the instrument
with the sampler);
application of samples on the gel plate;
electrophoretic migration;
gel drying on the plate Peltier;
gel staining;
gel destaining;
gel drying;
densitometric reading.

7.3.1 ELECTROPHORESIS OF THE ALKALINE HEMOGLOBINS


Make reference to the PK111 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
Hemoglobins in the session to be set (See 6.1.2):

6. After selecting the session or sessions confirm by clicking on


the button .

7. Select the options of the analysis startup and begin the session
by clicking on the button Hemoglobins (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.
10. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

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7.3.1 ELECTROPHORESIS OF THE ACID HEMOGLOBINS


Make reference to the PK112 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the stain solution 1 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
Acid Hemoglobins in the session to be set (See 6.1.2):

6. After selecting the session or sessions confirm by clicking on


the button .

7. Select the options of the analysis startup and begin the session
by clicking on the button Acid Hemoglobins (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

III. Operations at the end of the analysis


9. Remove the dry gel plate from the holder.
10. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
11. Remove the disposable plate sample from the plate sample
support and delete them.

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132 7. METHODS OF THE ANALYSIS
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7.4 ELECTROPHORESIS OF THE LIPOPROTEINS

The instrument runs the following steps of the methods, in an automated way:

reading of the patients ID from tubes and sampling (only the instrument
with the sampler);
application of samples on the gel plate;
electrophoretic migration;
gel drying on the plate Peltier;
gel staining;
gel destaining;
gel drying;
densitometric reading.

7.4.1 ELECTROPHORESIS OF THE LIPOPROTEINS 13 TEST


Make reference to the PK116 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the stain solution 3 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
Lipoproteins 13 in the session to be set (See 6.1.2):

6. After selecting the session or sessions confirm by clicking on


the button .

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7. Select the options of the analysis startup and begin the session
by clicking on the button Lipo 13 (See 6.2.2).

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After drying take the gel holder from the instrument and clean
the reverse part of the gel plate with cotton wool soaked in
ethanol.

10. Place the gel holder in its parking.

11. Click on the button Restart and wait until the end of the
analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.4.2 ELECTROPHORESIS OF THE LIPOPROTEINS 26 TEST


Make reference to the PK116 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the stain solution 3 (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
Lipoproteins 26 in the session to be set (See 6.1.2):

6. After selecting the session or sessions confirm by clicking on


the button .

7. Select the options of the analysis startup and begin the session
by clicking on the button Lipo 26 (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After drying take the gel holder from the instrument and clean
the reverse part of the gel plate with cotton wool soaked in
ethanol.

10. Place the gel holder in its parking.

11. Click on the button Restart and wait until the end of the
analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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136 7. METHODS OF THE ANALYSIS
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7.5 ELECTROPHORESIS OF THE ISOENZYMES

The methods of the isoenzyme electrophoresis carried out by the instrument


INTERLAB G26 are made by semiautomatic procedures.

The automated steps are:


reading of the ID patients from the tubes and sampling (only the
instrument with the sampler);
application of the samples on the gel plate;
electrophoretic migration;
gel destaing;
gel washing;
gel drying;
gel reading.

7.5.1 ELECTROPHORESIS OF THE LACTATE DEHYDROGENASE


ISOENZYMES (LDH)
Make reference to the PK125 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the tank 3 filled with the acetic acid solution
at 5% (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the gel holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
LDH Isoenzymes in the session to be set (See 6.1.2):

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_________________________________________________________________________________

6. After selecting the session or sessions confirm by clicking on


the button .

7. Select the options of the analysis startup and begin the session
by clicking on the button LDH Isoenzymes (See 6.2.2).

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After the migration take the holder with the gel from the
instrument and by using the Easy Mask run the external steps
manually.

10. Place the gel holder in its parking.

11. Click on the button Restart and wait until the end of the
analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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138 7. METHODS OF THE ANALYSIS
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7.5.2 ELECTROPHORESIS OF THE CK ISOENZYMES


Make reference to the PK117 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the tank 3 filled with the acetic acid solution
at 5% (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
CPK Isoenzymes in the session to be set (See 6.1.2):

6. After selecting the session or sessions confirm by clicking on


the button .

7. Select the options of the analysis startup and begin the session
by clicking on the button CPK Isoenzymes (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After the migration take the holder with the gel from the
instrument and by using the Easy Mask run the external steps
manually.

10. Place the gel holder in its parking.

11. Click on the button Restart and wait until the end of the
analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.5.3 ELECTROPHORESIS OF THE ALKALINE PHOSPHATASE


ISOENZYMES (ALP)
Make reference to the PK113 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the tank 3 filled with the acetic acid solution
at 10% (See 5.1.2).
2. Prepare the plate sample support(See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
ALP Isoenzymes in the session to be set (See 6.1.2):

6. After selecting the session or sessions confirm by clicking on


the button .

7. Select the options of the analysis startup and begin the session
by clicking on the button ALP Isoenzymes (See 6.2.2).

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8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After the migration take the holder with the gel from the
instrument and by using the Easy Mask run the external steps
manually.

10. Place the gel holder in its parking.

11. Click on the button Restart and wait until the end of the
analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.6 CRYOGLOBULINS

The methods for the cryoglobulins carried out by the instrument INTERLAB
G26 are made by semiautomatic procedures.

The automated steps are:


reading of the ID patients from the tubes and sampling (only the
instrument with the sampler);
application of the samples on the gel plate;
electrophoretic migration;
gel drying on the Peltier plate;
gel stain;
gel destaing;
gel drying;

7.6.1 CRYOGLOBULINS 2 TESTS

I. Preparation of the analysis


1. Check the level of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support (See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
Cryoglobulins 2 in the session to be set (See 6.1.2):

6. After selecting the session or sessions confirm by clicking on


the button .

7. Select the options of the analysis startup and begin the session
by clicking on the button Cryo 2 (See 6.2.2).

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_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After the migration take the holder with the gel from the
instrument and run the external steps manually.

10. Place the gel holder in its parking.

11. Click on the button Restart and wait until the end of the
analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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7.6.2 CRYOGLOBULINS 4 TESTS

I. Preparation of the analysis


1. Check the level of the stain solution 2 (See 5.1.2).
2. Prepare the plate sample support (See 5.1.4).
3. Prepare the migration chamber (See 5.1.5).
4. Prepare the holder (See 5.1.6).

II. Starting of the analysis


5. In the Select Methods Elfolab menu choose the method
Cryoglobulins 4 in the session to be set (See 6.1.2):

6. After selecting the session or sessions confirm by clicking on


the button .

7. Select the options of the analysis startup and begin the session
by clicking on the button Cryo 4 (See 6.2.2).

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7. METHODS OF THE ANALYSIS 145
_________________________________________________________________________________

8. Place the racks in the instrument and make sure that all ID
patients are acquired (For the instrument with the sampler)
(See 5.1.7). Wait for the end of the analysis.

9. After the migration take the holder with the gel from the
instrument and run the external steps manually.

10. Place the gel holder in its parking.

11. Click on the button Restart and wait until the end of the
analysis.

III. Operations at the end of the analysis


12. Remove the dry gel plate from the holder.
13. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.
14. Remove the disposable plate sample from the plate sample
support and delete them.

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146 7. METHODS OF THE ANALYSIS
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7.7 CSF ISOELECTRIC FOCUSING

The method for the CSF isoelectric focusing, carried out by the INTERLAB
G26 instrument, has the electrophoretic migration as an unique automated
step.

Make reference to the PK135 procedure of the CD included in the kit box.

I. Preparation of the analysis


1. Prepare the gel plates as described in the procedure of the
method.

II. Starting of the analysis


2. In the Select Methods Elfolab menu choose the method
CSF IEF in the session to be set (See 6.1.2):

3. After selecting the session or sessions confirm by clicking on


the button .

4. Select the options of the analysis startup and begin the session
by clicking on the button CSF IEF (See 6.2.2).

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7. METHODS OF THE ANALYSIS 147
_________________________________________________________________________________

5. After the migration take the holder with the gel from the
instrument and run the external steps manually as described in
the procedure.

III. Operations at the end of the analysis


6. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.

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148 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

7.8 SDS PROTEINURIE

The methods for the SDS proteinurie run by the instrument INTERLAB G26
are the automated steps:
electrophoretic migration;
drying of the gel plate Peltier;
gel staining;
gel destaing;
gel drying;

Make reference to the PK122 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Check the level of the tank 3 filled with the acid violet solution
(See 5.1.2).
2. Prepare the migration chamber (See 5.1.5).
3. Prepare the gel plates as described in the procedure of the
method.

II. Starting of the analysis


4. In the Select Methods Elfolab menu choose the method
SDS in the session to be set (See 6.1.2):

5. After selecting the session or sessions confirm by clicking on


the button .

6. Select the options of the analysis startup and begin the session
by clicking on the button SDS Urines (See 6.2.2).

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7. METHODS OF THE ANALYSIS 149
_________________________________________________________________________________

7. Wait for the end of the analysis.

III. Operations at the end of the analysis


8. Remove the dry gel plate from the holder.
9. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.

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150 7. METHODS OF THE ANALYSIS
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7.9 ELECTROPHORESIS OF THE -AMYLASE ISOEZYMES

The method for the -amylase isoenzymes run by the INTERLAB G26
instrument has the electrophoretic migration as an unique automated step.

Make reference to the PK134 procedure of the CD included in the kit.

I. Preparation of the analysis


1. Prepare the gel plates as described in the procedure of the
method.

II. Starting of the analysis


2. In the Select Methods Elfolab menu choose the method
Alpha Amylase in the session to be set (See 6.1.2):

3. After selecting the session or sessions confirm by clicking on


the button .

4. Select the options of the analysis startup and begin the session
by clicking on the button Alpha-Amylase (See 6.2.2).

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7. METHODS OF THE ANALYSIS 151
_________________________________________________________________________________

5. After the migration take the holder with the gel from the
instrument and run the external steps manually as described in
the procedure.

III. Operations at the end of the analysis


6. Remove the soaked sponges by the migration chamber and
remove and clean the slots with a paper dampened with the
distilled water and dry them.

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152 7. METHODS OF THE ANALYSIS
_________________________________________________________________________________

7.10 1-ANTITRYPSINE ISOELECTRIC FOCUSING

The method of the 1-antitrypsine isoelectric focusing run by the INTERLAB


G26 instrument is made by a semiautomatic procedure.

The automated steps are:


reading of the ID patients from the tubes and sampling (only the
instrument with the sampler);
application of the samples on the gel plate;
electrophoretic migration;

Make reference to the PK138 procedure of the CD included in the kit box.

I. Preparation of the analysis


1. Prepare the gel plates as described in the procedure of the
method.

II. Starting of the analysis


2. In the Select Methods Elfolab menu choose the method
Alpha1-Antitrypsin in the session to be set (See 6.1.2):

3. After selecting the session or sessions confirm by clicking on


the button .

4. Select the options of the analysis startup and begin the session
by clicking on the button Alpha 1 Anti-Tripsyn (See 6.2.2).

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7. METHODS OF THE ANALYSIS 153
_________________________________________________________________________________

5. After the migration take the holder with the gel from the
instrument and run the external steps manually as described in
the procedure.

III. Operations at the end of the analysis


6. Remove the soaked sponges by the migration chamber and
clean the slots with a paper dampened with the distilled water
and dry them.

Users Manual Ver. 2.0


8. MAINTENANCE

8.1 DAILY CLOSING PROCEDURE

After using the instrument clean with damped paper of distilled water the
electrodes of the migration chamber.

WARNING

Clean the parts that might be contaminated by biological liquids with a


cloth soaked in a solution of sodium hypochlorite.

8.2 WEEKLY CLEANING PROCEDURE

Soak the migration chamber in water for 30 minutes. First rinse it under
the running water, then with the distilled water and finally dry it with the
blotting paper.
Empty the tanks of the stain solutions, rinse them under the running water,
after with distilled water and finally let them dry to air before replacing
them in the instrument.
Clean the Peltier plate with a soft paper soaked in ethyl alcohol.

CAUTION

Be careful do not fit alcohol into the plate holes during cleaning the
Peltier plate.
9. PROBLEMS AND SOLUTIONS 155
_________________________________________________________________________________

8.3 DISINFECTION OF THE INSTRUMENT

Before any movement and any assistance of the Technical Support Staff, the
instrument must be disinfected in accordance with the provisions of the
disinfection Protocol .

8.4 WASTE DISPOSAL

For the waste disposal work in accordance with the current laws.
For further information on safe waste disposal, on the associated risks and the
precautions to be taken, including special protective measures, make
reference to the safety sheet of the reagents available from the Technical
Service of the supplier.

WARNING

During the maintenance operations and the closing operations of the


waste and biological waste tanks, and during their handling, wear all the
provided protective equipment.

Users Manual Ver. 2.0


9. PROBLEMS AND SOLUTIONS

9.1 ALARM

During the use alarm messages can be displayed on the interface PC. They refer to
a problem of the instrument or a mistake made by the operator.
In most cases you can use the dialogue window, with two or three buttons depending
on the kind of error, as shown in the table below.

Click on the button:


Ignore to ignore the problem and continue;
Retry to solve the problem;
Cancel to cancel the operation in progress.

Solutions
Problem Alarm Notes
Ignore Retry Cancel

The X Motor not completed


X Axis problem
the work on time.
The Y Motor not completed
Y Axis problem
the work on time.
TheZ Motor not completed
Z Axis problem
the work on time
The Rotor not completed
Rotor problem
the work on time.
The Diluter not completed
Diluter problem
the work on time.
Crash needle The needle has
encountered an obstacle. If
the needle fall from the Arm
you should place it
manually in the parking
position and select Abort.
Otherwise select Retry.
9. PROBLEMS AND SOLUTIONS 157
_________________________________________________________________________________

Solutions
Problem Alarm Notes
Ignore Retry Cancel

The Arm has failed to


engage the subject. If the
object is absent, you have
Engaging arm to select manually the
desired location and try
again, otherwise select
Cancel.
The Arm is already
Engaged arm
engaged.
EEPROM Firmware Error.
You tried to take an object
parked in a verse incorrect.
Incorrect verse You have to insert the
object with the correct
direction and try again.
The Grid is jammed and can
Grid
not be opened.
The arm does not feel the
presence of the object and
Free arm
can not complete the
deposit.

The error is
Empty tube Liquid Tube insufficient. reported for
the IFE only.
The Sample Support is not
Sample Support inserted. Insert the Samples
Support and Retry.
Overtemperature The Migration Chamber has
Peltier exceeded 80 C.
Overtemperature The Drying Chamber has
drying chamber exceeded 80 C
No Liquid in the Washing
Washing needle
Tank Needle/Applicator. Fill
tank
the tank needed.
The Treatment Chamber
has not been filled. Control
Staining tank
the level of the tank and
Retry.

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158 9. PROBLEMS AND SOLUTIONS
_________________________________________________________________________________

Solutions
Problem Alarm Notes
Ignore Retry Cancel

High voltage Power Supply


Power supply problem. The Analysis can
not proceed.
There is only
Not Presence of the gel in the button "OK"
the Migration Chamber. to verify that
The gel may be missing or the user has
Migration incorrectly inserted. taken what it
chamber vacuum The analysis has been has appened.
aborted. The following
Insert correctly the gel and analysis
restart. continue
regularly.
The Peltier did not reach in
Time Out Peltier the correct Time the set
temperature.
The Object in the Arm is not
Object arm error congruent with the deposit
required position.
Cache memory Internal error
Empty tank Tank not full.
Full tank Tank already full.
The machine can
not handle the The machine can not handle
movement of the the movement of the Grid.
Grid.
The Rack is not correctly
No Rack inserted. Insert the rack
correctly and Retry.
ROM Hardware problem.
ROM Retry or contact Technical
Support.
Input / Output Problem.
Input/Output Retry again or contact
Technical Support.

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9. PROBLEMS AND SOLUTIONS 159
_________________________________________________________________________________

Solutions
Problem Error message Notes
Ignore Retry Cancel

There is only
the button "OK"
to verify that
the user has
Washing Absence Liquid in the taken what it
applicators washing tank. has appened.
The following
analysis
continue
regularly.
Cover open Cover open!
Absence Liquid of washing
Washing needle Needle. Fill the tank of
distilled water and Retry.
Internal logic
Internal logic error.
error.
Volume Required
Volume Required wrong.
wrong.

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160 9. PROBLEMS AND SOLUTIONS
_________________________________________________________________________________

9.2 TROUBLESHOOTING

Possible
Problem Solutions
cause

The instrument is off. Turn on the instrument.


The instrument does
not connect to the
The serial cable is not Connect the serial cable and restart
Personal Computer
connected. the instrument.
Wrong selection of the gel. Select the holder correctly and
restart the analysis.

Wrong insertion of the gel in the Make sure the gel is inserted in the
holder. holder correctly and restart the
analysis.

The migration chamber sponges Make sure the sponges is inserted


The instrument does
are: missing or not correctly in the migration chamber slots
not execute the
inserted. correctly and restart the analysis.
migration .
Make sure the cover is closed
Cover block activated. completely.

Contact the Technical Assistant.


The migration chamber is
malfunctioning.

WARNING

 During the sampling the needle can obstruct. In this case on the PC interface
a window will display needle obstructs.
 For cleaning the needle use the iron wire and place it in a container for any
liquid seepage. The needle is POTENTIALLY INFECTED, so use all the
required personal equipment for protection and observe all the safety
measures and PAY ATTENTION.

Users Manual Ver. 2.0


10. INDEX

Lipoprotein E. ........................... 132


A Protein E. ................................... 83
Elfolab
Accessories E. installation .............................. 44
A. assembly ............................... 34 E. starting ................................... 70
Inside A. .................................... 54 Environmental requirement ............ 26
Ouside A. ................................... 24
Posizione A. .............................. 24
Analysis G
A. starting . ................................. 54
Applicators ..................................... 59 Gel
Arm ............................................... 21 G. preparation ............................ 63
Gel holder ....................................... 24
B General warnings ............................. 6

Box content checking ............... 27,30


I
C Holes for the handles ..................... 19
Hood ............................................... 19
Characteristics ............................... 14
Connections
PC C. ......................................... 35 I
Electricity grid C. ....................... 35
Controls ......................................... 69 Identification plate .......................... 11
Cover ............................................. 19 Immunofixations ........................... 102
Inside of the instrument .................. 21
Installation
D I. of the instrument ..................... 34
I. of the software ......................... 37
Database ....................................... 71 Instructions
Description of the instrument ......... 19 I. for movement .......................... 51
I. for transport ............................. 52
I. for storage ............................... 52
Interferences .................................. 10
E Interlab G26 software
I. starting .................................... 74
Electrophoresis I. application installation ............. 47
-amylase isoenzyme E. ......... 150 Isoelectric focusing
Cryoglobulin E. ........................ 142 CSF I. ....................................... 146
Hemoglobins E. ....................... 128 1-Antitrypsin I. ........................ 152
Isoenzyme E. ........................... 136
162 10. INDEX
_________________________________________________________________________________

L R
Labels .............................................. 9 Responsibility ................................. 10
Limitations ..................................... 10
Location ......................................... 34
S
M Safety
Biological and Chemical S. .......... 7
Main panel ..................................... 74 Electrical S. .................................. 8
Maintenance ................................ 154 Mechanical S. ............................... 7
Manual Fire S. .......................................... 7
M. keeping ................................. 12 Serial port ....................................... 19
Guide at M. ................................ 13 Software ......................................... 70
Purpose and contents of the M. . 12 Starting ........................................... 54
Methods ......................................... 83 System configuration ...................... 26
Migration chamber ......................... 24 Switch ............................................. 19

O T
Operation principle ........................ 14 Technical features .......................... 17
Outside instrument ........................ 19 Trademarks .................................... 10

P U
Parking Unpacking ...................................... 27
Applicator P. .............................. 21
Gel holder P. ............................. 21
Preparation W
Gel P. ........................................ 63
Instrument P. ............................. 54 Wells
Migration chamber P. ................ 61 Immunofixation diluent W. .......... 21
Outside tank P. .......................... 54 Needle washing W. .................... 21
Racks P. .................................... 67 Applicator washing W. ................ 21
Sample plate support P. ............ 60 Windows 7 ...................................... 41
Stain solution tank P. ................. 57 Windows Vista ................................ 37
Problems and solutions ............... 156

Users Manual Ver. 2.0

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