D io n e x Driving Innovation

in a G lobal M arket

DX-500
 Dionex Ion Chromatography
Dionex continues to strengthen the performance
of the DX-500 Ion Chromatography (IC) System with
the addition of new automation products, innovative
technologies and design enhancements. New and
improved eluent delivery systems, automated eluent
generation and intelligent chromatography data
handling give you an ion chromatography system that
is versatile, reliable, and accurate. All system operations
from eluent generation to sample preparation are
automated, resulting in an integrated laboratory that
operates faster and more efficiently.
High Performance
The DX-500 IC offers the highest level of
performance of any IC system on the market today. Its
superior sensitivity and reproducibility is unmatched.
Significant developments like the improved eluent
delivery system of the GP50 pump, the advent of the
EG40 Eluent Generator, and the introduction of the
AutoSelect AS50 Autosampler make the DX-500 IC
™
system a state-of-the-art ion chromatograph with
superior analytical performance.
DX-500 IC System Module and Accessory Options
EO1 GP50 IP25 EG40
Eluent/Solvent Gradient Pump Isocratic Pump Eluent
Organizer Generator
Standard or Microbore
Superior Technology
The DX-500 system represents over two decades
of Dionex research, innovation, and expertise in
ion analysis. Our column technology is developed
in response to our customers’ application needs and
is optimized for use with Dionex ion chromatography
systems. Dionex suppressor technology, now in its
fifth generation, is unmatched in terms of performance
and reliability. With our exclusive “Just Add Water”
technology, which provides automatic eluent generation,
Dionex has brought unprecedented power and
convenience to ion analysis.
Sensitive and Accurate Results
Every chemist needs sensitive and accurate results
over a wide concentration. The DX-500 IC system
provides reproducible ultra-trace analyses and
identification of components in the most complicated
sample matrices. For applications with diverse
levels of analytes, the DX-500 can monitor ions
over four orders of linear range.
Application Flexibility
In need of detecting low levels of inorganic anions
and cations, organic acids, amines, and transition
metals? How about anions in concentrated bases
and cations in concentrated acids? All of these analyses,
and more, can be performed with the versatile
DX-500 IC system.
LC20 LC25 LC30
Chromatography Chromatography Chromatography
Enclosure Oven Oven

LC20 LC25 LC30 AS40 AS50 AS50

Chromatography Chromatography Chromatography Autosampler Autosampler Compartments
Enclosure Oven Oven Thermal or
Chromatography

ED40 CD20 AD25 PeakNet

Electrochemical Conductivity UV-Vis Absorbance Chromatography
1 2 Detector Detector Detector Workstation
The DX-500 Ion Chromatograph Versatile
Fast Perform a wide range of applications using multiple
Separate, detect, identify, and quantify multiple modes of detection. DX-500 modules are engineered to
ions in a matter of minutes. With a DX-500 system every be interchangeable. Different configurations of columns
stage of the analysis process is automated, providing and detectors allow for any IC application to be run.
maximum laboratory efficiency. Automate ion chromatography analysis on the DX-500
system with intelligent autosamplers and powerful
Easy to Use
PeakNet chromatography software. Expansion from
All of the DX-500 system modules and accessories
a single to dual channel system is often just the
are designed for ease of use. Built-in user friendly
addition of another pump.
diagnostics and help screens; bright, easy-to-read front
panels, and logical menus facilitate user operation.
Innovative modules like the EG40 Eluent Generator
and AS50 Autosampler automate difficult or mundane
operations for further simplicity. Eliminate eluent
preparation and the handling of acids and bases
with the EG40, and use the AS50 to simplify sample
management and preparation.
 Superior Technologies
“Just Add Water” Technology
This breakthrough concept means that you do not
have to prepare eluent or regenerant solutions to do
IC — you just add water.
The heart of “Just Add Water” is the
combination of membrane based technology
and electrolysis. Using the EG40 Eluent
Generator, eluent is created continuously,
on-line, from water. In the SRS-ULTRA ,
regenerant ions are produced from
recycled eluent.
“Just Add Water” technology pro-
vides IC results that are fast, easy, and
powerful. This technology is just one
example of how Dionex continues to improve
the field of ion analysis.
Suppressor Technology
Suppressors work by reducing the conductivity
of the eluent, while enhancing the conductivity of the
analytes. This dramatically improves sensitivity, stability
and dynamic range for anions and cations.
Dionex was the first company to introduce IC
instrumentation utilizing chemical suppression; that was
25 years ago. Since then, we have continued to lead the
industry in innovative suppression technology. Our first
suppressors were made from ion exchange resins used
as a packed bed. Packed bed suppression allowed iso-
cratic separations for samples with simple matrices. By
the mid 80’s IC application demands inspired the inven-
tion of gradient capable, high capacity, continuously
regenerated, MicroMembrane® Suppressors for use
with high capacity columns. External regen-
erant pumps were eliminated by using
AutoRegen® devices to improve reliabili-
ty. In the early 1990’s, suppression was
made even more powerful with a
®
technical breakthrough; the Self-
Regenerating Suppressor® (SRS).
The SRS was the first generation of
continuous, electrically regenerated
membrane-based suppressors, that
allowed demanding gradient and
high capacity separations. SRS technology made
IC easier because operation without external regenerant
solutions or pumps was now possible.
Today, Dionex has taken suppressor technology
a step further, by improving sensitivity and reliability
with SRS-ULTRA suppressors. Many benchmarks in sup-
pression technology have been set by Dionex over the last
20 years, and you can be assured we will continue to
develop new suppression products and technologies in
the years to come.
SRS-ULTRA suppressors
provide superior performance
with excellent baseline
stability and low detection
limits for gradient
and isocratic separations.

Dionex Commercialized MicroMembrane® Suppressor 2 mm MicroMembrane SRS-II

the First Packed Bed Suppressors
Small Fiber Suppressor AutoRegen® SRS-I AutoSuppression® SRS-ULTRA
et. al.
Publish First 1975 1981 1985 1987 1991 1992 1997 1998 2000
Suppressed
Conductivity
IC Paper Packed Bed Suppression MicroMembrane Self-Regenerating SRS-ULTRA
– Restricted to isocratic operation with low capacity columns Suppression Suppression – The ultimate in reliability
– Required off-line regeneration and external regenerant pumps – Expanded suppression – Uses electrolysis of water and performance
– Required external acid/base regeneration solutions to gradient operation to supply regenerant ions – 20 years of breakthroughs
– Expanded suppression to use – No external acid/base solutions and refinements in
high capacity columns or pumps required suppression design
– On-line, continuous regeneration – High capacity for all applications
including gradient operation
– Allowed use of high
capacity columns
– Plug-in and “Just Add Water”
3 4
EG40 Eluent Generator
The EG40 makes IC easier than ever by generating
high-purity IC eluents on-line using electrolysis of
deionized water. On-line eluent generation for IC
eliminates the eluent contaminants that cause gradient
baseline shifts, resulting in higher sensitivity, improved
resolution and better peak integration. The EG40
delivers outstanding eluent reproducibility, which
provides excellent retention time stability. There are
no chemicals to handle or reagents to prepare. The
entire process is automated and uses only water.
14818
Hydroxide eluents produced by the EG40
are free of carbonate interferences, and
as a result baseline shift is eliminated
allowing for enhanced resolution
and higher sensitivity.
Column Technology
Dionex offers a wide range of polymeric IC columns
for various modes of separation including ion exchange,
ion exclusion, ion pairing, and reversed phase. By
using Dionex columns for your IC work, you are
benefiting from many years of expertise in polymeric
resin technology and IC application experience.
All resins and columns are developed and produced
by Dionex. Dionex IC columns are constructed from
non-metallic, non-corrosive PEEK components to
eliminate contamination due to metal corrosion. A wide
range of low, medium, and high capacity columns
designed for fast analysis times or optimum resolution
of complex samples are available.
 Advanced Detection Capabilities
Expand your analytical capabilities by using multiple
detectors configured on a single DX-500 IC system.
Dionex detectors are designed with user friendly service
diagnostics and on-screen directions for easy cell
calibration. All of our detectors provide digital
signals and are autoranging when used with the
PeakNet Chromatography Workstation. The modular
design of the DX-500 IC allows for one or more of the
following detectors to be configured on a system.
ED40 Electrochemical Detector
The ED40 is our most powerful and versatile
detector, offering three modes of electrochemical
detection. It provides the power of suppressed
conductivity detection that is offered by the CD20,
as well as two forms of electrochemical detection;
dc amperometry and integrated amperometry.

CD20 Conductivity Detector

Detection techniques like colorimetry, nonsuppressed
conductivity, and UV-Vis absorbance usually cannot
obtain the low detection limits and signal-to-noise
performance that can be achieved using the DX-500 IC
system with suppressed conductivity detection. The
CD20 is a microprocessor-driven precision conductivity
detector. Digital autoranging and temperature com-
pensation combined with built-in control for the SRS
Self-Regenerating Suppressors provide unsurpassed 13740

signal-to-noise, stability, linearity, and dynamic range. Determine common cations using the ED40 with SRS Self-Regenerating
Cation Suppression, and the EG40 Eluent Generator.

13026

The compatibility of the ED40 using integrated amperometry is a

powerful tool for the separation of complex mixtures of mono-,
di-, oligo-, and polysaccharides.
13047-01

Suppressed conductivity detection with the CD20 can determine trace

level anions in the presence of higher level anions in the same run.

5 6
 VARIOUS COMPONENTS DETECTED
BY ION CHROMATOGRAPHY
AD25 Absorbance Detector
The AD25 is a dual beam, variable wavelength detector. It DETECTION
MODE ANALYTES

features both a deuterium and tungsten light source to ensure Conductivity

optimum performance across a broad wavelengh range of CD20/ED40 • Inorganic anions
• Inorganic cations
190-800 nm, with extremely low baseline noise, accurate • Chromate
• Disinfection by-products
response and exceptional wavelengh accuracy. Validation is
• Ethanolamines
simplified by using a built in holmium oxide filter for fast, sim- • Amines
• Ionic surfactants
ple verification of wavelengh accuracy. • Organic acids
• Polyphosphates
• Sulfonates
• Azide
• Borate
• Perchlorate

DC
DS3 and Amperometry
standard ED40 • Cyanide
conductivity cell • Sulfide/sulfite
• Iodide

Integrated
Amperometry
Amperometry cell ED40 • Carbohydrates
• Alcohols/glycols
• Amines
• Catecholamines
The AD25 UV-Vis absorbance detector extends the capability of
• Phenols
the DX-500 to include transition metals analysis.
• Sulfides
• Thiols
• Sugar alcohols
• Amino acids

UV/VIS
AD25 • Transition metals
• Nitrate/nitrite
• Silicate
• Chelating agents
• Nonionic surfactants
• Lanthanide metals
• Stains and dyes
• Pesticides/herbicides
• Drugs/pharmaceuticals
• Nucleic acids
• Purines/pyrimidines
• Proteins/peptides

11873
 High Performance Pumps
Dionex offers two chromatographic pumps for the Precise Eluent Proportioning
DX-500 IC system. Our high performance gradient The GP50 gradient pump provides precise eluent
pump, the GP50, is a sophisticated eluent delivery proportioning regardless of system back pressure or
system designed to pump mixtures of up to four solvent viscosity. Low void volumes result in sharp
mobile phase components, and either perform gradients with minimal delays.
isocratically, or deliver linear or exponential gradients.
Simple Maintenance and Calibration
Accurate proportioning and precise eluent delivery
Built-in user-service menus with on-screen directions
ensure consistent results—day in and day out.
make pump calibration simple. The GP50 and IP25
The IP25 isocratic pump is used exclusively for
pumps are designed with an easy access housing
isocratic eluent delivery.
mechanism that slides out for routine maintenance.
Superior Flow Control
Both the GP50 and IP25 have a Dual Digital Signal
Processor (DSP) control to provide exceptional flow rate
accuracy for optimal run-to-run reproducibility and area
precision. DSP ensures superior flow stability over a
wide range of backpressures.

14283

The GP50 provides low void volumes which result in sharp and precise
gradients as demonstrated by these 1% steps.

14285

The GP50 and IP25 pumps provide exceptional flow control for
run-to-run reproducibility (0.1% standard bore, 0.2% microbore).

7 8
Rugged PEEK Design
Dionex continues to improve pump design to ensure
durability for challenging laboratory applications.
For long life and minimal maintenance, the GP50
and IP25 pumps are constructed of rugged PEEK
components. The PEEK-based flow paths and
mechanical components of the GP50 and IP25 are
chemically inert. PEEK flow paths guarantee that the
metal contamination commonly found when using
stainless steel pumps is eliminated.
Eluents like dilute sulfuric acid and sodium
hydroxide cannot corrode pump components
and tubing due to chemically inert,
PEEK-based flow paths.
Extend Your IC Capabilities with Additional Options
Vacuum Degas Option
The GP50 and IP25 pumps feature a built-in vacuum
degas option to provide in-line degassing for optimum
reproducibility, low backgrounds, and protection of
solvents from contamination.
Microbore or Standard Bore Configurations
To optimize trace analysis and amino acid
applications, the GP50 and IP25 pumps are offered
in microbore configurations. Benefits of the microbore
configuration include increased sensitivity, lower
detection limits, and reduced eluent consumption.
 Powerful Automation
The demand for increased efficiency, sample through-
put, and documentation is playing an increasingly
important role in laboratories today. To maximize the
capabilities of the DX-500 IC system, automation tools
such as a PeakNet Chromatography Workstation
or the AutoSelect AS50 Autosampler can be added for
continuous unattended operation. With PeakNet,
the entire chromatography system—from sample
dilution to eluent generation—can be controlled from
one software package.
Control panels for each ion chromatograph module (pump, detector,
autosampler, etc.) can be established to display instrument conditions.
Graphical icons alow intuitive remote operation, including running a
sequence or schedule of injections.
9 10
PeakNet Chromatography W orkstation
PeakNet 6 provides a secure, stable computing environ-
ment to fully address GLP/GMP. Flexible system securi-
ty features allow laboratories to impose appropriate data
control based on their work environment and individual
needs. Whether you wish to work in a completely open
and accessible environment, or operate with restricted
access and controlled privileges, PeakNet 6 gives you the
power to manage your lab’s IC data.
The PeakNet
Sequence Browser
allows you to
perform batch oper -
ations and access
results.
PeakNet conveniently displays integration views of all injections.
AutoSelect AS50 Autosampler Automate sample dilution and reagent addition
The AutoSelect AS50 is a versatile, accurate, random- with the AS50 Sample Preparation option. The Column
vial access autosampler. Designed for reliability and Selection Valve option saves time by automatically
increased sample throughput, the AS50 delivers high switching between two independent applications
precision and low carryover to guarantee reproducible on the same IC system. The AS50 supports two
results with injection volumes ranging from 1 µL to chromatography enclosure options to house and
1.5 mL. Metal-free flow paths ensure sample integrity. protect IC components: the Thermal Compartment
provides improved site-to-site reproducibility for high
sensitivity work, and the Chromatography
Compartment organizes components for routine work
not requiring a temperature controlled environment.
AS50 Thermal
Chromatography
Compartment
AS40 Automated Sampler
configured with The AS40 Automated Sampler is a rugged,
the Column
Switching Option. economical, sample loading device for the DX-500.
This autosampler features a chemically inert, metal-free
flow path. The AS40 design makes operation and
maintenance simple.
Discover How Powerful IC Can Be For more information, demonstrations,
and no-obligation quotations, call your
Bring the full power of IC to your laboratory.
Dionex representative.
For complete information on the DX-500, contact
your nearest Dionex representative. Corporate Headquarters
Dionex Corporation
Guaranteed Quality 1228 Titan Way
P.O. Box 3603
Purchasing a Dionex product is an investment in
Sunnyvale, CA94088-3603
quality. All Dionex products are designed, developed, TEL: (408) 737-0700
and manufactured under an NSAI registered ISO 9001 FAX: (408) 730- 9403

Quality System and warranted against defects in

Salt Lake City Technical Center
materials and workmanship. (Extraction Specialists)
1515 West 2200 South, Suite A
The Dionex Commitment to Customer Satisfaction Salt Lake City, UT 84119-1484
As a Dionex customer, you have access to worldwide TEL: (801) 972-9292
FAX: (801) 972-9291
customer service and support. Our Customer Service
organization has earned a reputation for timely U.S. Regional Offices
and professional service, expert training, and skilled Sunnyvale, CA (408) 737- 8522
Westmont, IL (630) 789- 3660
applications assistance.
Atlanta, GA (770) 432- 8100
Houston, TX (281) 847-5 652
A Complete Selection of Separation Science Pr oducts Marlton, NJ (856) 596- 0600
In addition to ion chromatography systems and
International Subsidiaries
modules, Dionex offers a complete selection of products
Austria (01) 616 51 25
for your laboratory, including: Belgium (015) 203800
• HPLC systems Canada (905) 844-9650
France 01 39 46 08 40
• Chromatography data systems
Germany 06126 991-0
• On-line process analyzers Italy (06) 66 51 50 52
• Accelerated Solvent Extraction systems Japan (06) 6885 -1213
Netherlands (0161) 43 43 03
• Automation products
Switzerland (062) 205 99 66
• Columns, eluents, suppressors, and accessories United Kingdom (01276) 691722

Internet
www.dionex.com

Dionex products are designed, developed

and manufactured under an NSAI registered
ISO 9001 Quality System.
IonPac, AutoSuppression, AutoRegen, ASRS, SRS
and Self-Regenerating Suppressors are registered
trademarks and AutoSelect,MicroMembrane, MetPac,
and CarboPac are trademarks of Dionex Corporation.
Maltrin is a trademark of Grain Processing Corporation.
Specifications subject to change without notice.

IC | HP LC | EXTRACTION | PROCESS | AUTOM ATION © 1999 Dionex Corporation

LPN 0502-03 10K 11/99
Printed in U.S.A.