27/1/2018 MDCK.

2 ATCC ® CRL-2936™ Canis familiaris kidney; distal tubu

MDCK.2 (ATCC® CRL-2936™)

Organism: Canis familiaris, dog / Tissue: kidney; distal tubule / Disease: normal

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GENERAL INFORMATION CHARACTERISTICS CULTURE METHOD SPECIFICATIONS HISTORY DOCUMENTATION

Karyotype hyperdiploid canine cell line with a modal chromosome number of

91 with low polyploidy rate. Several unidentifiable marker
chromosomes were present in most of the cells examined.

Images

Derivation Cell line was derived by cloning (limited dilution) the parental cell
line MDCK (ATCC CCL-34).

Antigen Expression E-cadherin (epithelial cell adhesion molecule), expressed

Zona Occludens (ZO-1) (tight junction protein), expressed
fibroblast-specific protein (FSP), not expressed
cytokeratin (CK1, 4, 5, 6, 8, 10, 13, 18, 19),expressed
sialic receptors: alpha 2,3-galactose (avian)and alpha 2,6-
galactose (human); expressed

Genes Expressed Zona Occludens (ZO-1) (tight junction protein), expressed,E-

cadherin (epithelial cell adhesion molecule), expressed,fibroblast-
specific protein (FSP), not expressed ,cytokeratin (CK1, 4, 5, 6, 8,
10, 13, 18, 19),expressed,sialic receptors: alpha 2,3-galactose
(avian)and alpha 2,6-galactose (human); expressed

Virus Susceptibility Influenza A virus

Influenza B virus

Comments This cell line is susceptible to a wide range of influenza virus and
is sensitive to epsilon toxin of C. perfringens.

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