Date of experiment: 13 October 2017

Researchers:
Mac Jayvin Balanay
Eloisa Myra Balicha
Carl James Caballero
Ynna Cassandra Gelacio
Nicole Faye Gonzales
Jelouie Lacerna
Reamlyx Lachica
Eloiza Marie Manglugay
Raimiel Rivera
Claudine Valdez

Equipments:
Centrifuge
Filter paper

Reagents:
Diethanolamine

Milk sample test

2 ml pasteurized and spoiled milk sample were centrifuged for 10 min at 3000 rpm for
the separation of fat layer. Penetrated fat layer with pipette and samples was take out
(milk) from cream. Skimmed milk was filtered through a small, moist, blue band filter
and 0.1 ml of the filtrate mixed with diethanolamine (Bergmeyer 1974). This mixture
was applied over the immobilized glass surface and observed the yellow color formation
observed.