Disadvantages of Tissue Culture:

 The experiments involved in tissue culture are expensive

because the expensive machinery and reagents required,
 This technique is a complex procedure and it is has
varied procedures depending on the plant type.
 The experiments of tissue culture must be handled by
highly trained people as the procedure requires special
are and careful observation.
 As all the plants are genetically similar, there is
reduction is genetic diversity.
 There is no introduction of any new genes in the gene
pool of the whole stock.
 If a plant is susceptible to disease, all the plants of
this cloned stock will share this undesirable trait and
be susceptible to that particular disease.
 On a large scale production tissue culture methods is
very expensive as the cost of the equipments are very
high.
 The procedure depends of the type of species being
cultured; hence there is a need of trial and error method
for any new species if there is no review about that
species.
 Sometimes there is a possibility of error in the identity
of the organism after culture,
 If precautions are not taken the whole stock may be
contaminated or infected.
 Tissue culture, cloning decreases genetic variability.
 Monoculture is produced due to tissue culture and all the
progeny may be vulnerable to the same infections or
diseases.
 Samples of infected plants produce infected progeny; all
the plants of the stock must be carefully screened to
prevent culturing infected plants.
 Tissue culture is not successful with all plant species
usually because of the growth medium and some plants
produce secondary metabolites that might kill the
explants.
 Some plants are difficult to disinfect of fungal
infections.