Microbiology Post-test 2018

1. WHich of the following is true about the WOESE-KANDLERand WHEELIS taxonomic classification?
-2 and 4(it is phylogenetic and It is based on similarty in 16sRNA)
2. The first life form from where all living organisms evolve has this characteristic.
-anaerobic prokaryote
3. No normal flora
- Lungs
4. Laboratory involved in isolation of Mycotbacerium HIV and Ebola virus
-BSL 4
5. Pasteurization destroysteh sporeforming organisms at this temperature
-NON of these (choices: 60,5C, 12.3C and 62.7C)
6. Shank’s modification will stain
-CAPSULE
7. Stained by Dyar method
-cell wall
8. The resistance of spore to heata nd drying is due to this characteristic
- 1 and 3 (presence of dipicolonic acid in the cortex ; absence of water in the cortex)
9.which of teh followning methdo is used to determine the microbial density in a plate culture?
-choices: a. Use of calibrated loop B. Turbidimetric method c. Pourplate technique D. All of t
hese.
10. Which of the following microbes is best isolated at 30C
-all of these (Leptospira interogans, M. Phlei and Fungi)
11. Halophiles love to live in an envi with high salt content. Whcih of teh following does not belong?
-none of these(all are halophiles: Vibrio, Enterococcus and Staphylococcus)
12. WHich of the following are thermophilic?
-Campylobacter fetus, H. Pylori(?) and Pseudomonas aeruginosa
13. EMB that contains LACTOSE and MALTOSE
-Harris-Holt EMB (Levine;s EMB- lactose and glucose)
14. Growth factors:
-Cystine(), Phyto Albumin, Isovitale X(Neisseria and Haemophilus)
15. Gaspak Jar, Torbal ajr and Filde and McIntosh jare are:
-Anerobic jars
16. Used to maintain a capnophilic environment
-either (candle jar and JEMEC system)
17. It is added to maintain ANAEROBIOSIS in teh Chopped meat medium used for isol of C. Tetani
-VASPAR
18. Sodium desoxycholate (bile) is used to select w/c of the following grp of bacetria?
- gram negative intestinall bacilli
19. “Bread-crumb-like” or “fluffy ball” colones are produced by which of the following EUBACTERIA.
-1,2,3 and4 (F. Mortiferum, Legionella oakridgensis, Actinomyces naeslundii and My. Pneumoniae)
Microbiology Post-test 2018

20. V agar- Gadnerella vaginalis

21. Grow in Torbala nd Filde and McIntosh jar

-Clostridium spp.
22. Media for AST of anaerobic organisms
-Wilkins-Chalgrene agar
23. It contains bismuth and is therefore used to isolate teh Salmonella spp.?
-Wilson-Blair agar (MHA- aerobic; CLED agar-Proteus)
24. Test for Nutritionally -deficient Strep (Strep defectivus)
-Addition of pyridoxal/cysteine leads to growth
25. Techniques used to produce discrete (well-formed) colonies of Proteus vulgaris.
-all of these (5% agar, salt-free medium and use of Trimethoprim)
26. Old broth media, made anaerobic bya dding oil, will show “stallactite streamers”(pellicle growth.
-Yersenia pestis
27. Culture reminiscent of APPLE CIDER VINEGAR,also produces an Alkaline by product.
-Alkalineges faecalis
28. Medium used for cultivation of Bordetellaspp Contains Cystine, tellurium salt and blood.
-Regan-Lowe medium
29. Additive that will not make the medium selective
-Meat extract(enrichment)
30. CPE may be seen in this preparation
-a and b (direct smear and Pap’s smear-HIV)
31. Which of teh following are Chlamydial CPEs?
- miyagawa bodies, Halberstedfer-Prowazek and Levinthal-Cole-Lilie’s bodies
32. Used for morphologic identification of Spirillum minus
-Giemsa’s
33. Method of identification applies to the deemonstration of teh colonies of teh L-form microbes
-Diene’s
34. Media used for motility test?
-gelatin butt
35. The kaangawa strain of this bacterium is presumptively identifiedbin the WAGATSUMA BAP.
-Vibrio parahemolyticus
36. The O Ag of the family Enterobcateriaceae is described as follows.
-lipopolysaccharide; heat stable
37. All of family Enterobacteariceae belongs to this group
-Glucose fermenter; oxidase NEGATIVE
38. Which group do MOST members of teh family Vibrionaceae belong?
-sucrose fermenter; oxidase POSITIVE
39. Which of tthe foolowing organisms will give a positive Neeufeld(Quellung) test?
Microbiology Post-test 2018

-Strep pyogenes (hyaluronic acid capsule), Kleb pneumoniae, Strep pneumoniae

40. Which of the following organism will give a positive Urease test?
-Salmonella paratyphi(?)- K/A H2S+ no gas (Shigella: K/A H2S- no gas, Urease negative)
41. This bacterial virulnce lyses clot. it increases virulence by destroying the”wall” created by promoting
spread of teh microbe.
- Fibrinolysin

42. Differentiate S. Aureus from S. Epidermidis.

-Methicillin RESISTANCE, Nafcilin RESISTANCE (Penicillin resistance- solely for Staph Aureus)
43. K/A with gas (aka glucose efrmenter and glucose producer) in he TSIA.
Salmonella enteritidis (S. Typhi and S. Gallinarum- no gas; Shigella dysenteriaea- no gas)
44. Kinufman-White typing test- salmonella spp
45. “Blue milk” -Pseudomonas aeruginosa
46. Lancefield classification of Enterococci - Grp D strep (Grp A-S.pyo: Grp B-S. Agalactiaea, Grp C- S. D
ysagalactiaea, GrpF- S. Anginosus)
47. Gram positive anaerobic non--spore forming cocci
-none of these (it doesnt exist)
48. All of the ff produce the enzyme catalase except:
-Fusobacterium necrophorum
49. WHich of the following is a microaerophile?
-Treponema carateum
50. possible cause/ reason if the Gas pak jar fails to become warm 20mins after closure
-catalyst is old, catalyst is satd with moisture
51. The fermentation of glucose pathway produce the following products: acid, CO2 and alcohol.
-Heterolactice pathway
52. One of the following is a non-flagellated gram negative intestinal bacilllus.
-Klebsiella oxytoca
53. The Pyocin test is used to identify which of the following
-Pseudomonas aeruginosa
54. Teh clostridium toxin is responsible for necrotizing enterocolitis (Dambrand pigbel)
-Beta toxin
55. This non-cell wall containing orgnaism has NO sterol in its cell embrane.
-Acholeplasma
56. Treponemacae and Spirillaceae differ in this asbect
-nature of motility
57. Streptococcus pneumoniae is a hemolytic. However it will show Beta hemolysis if incubation is done
-Anaerobically
58. A strain of Haemophilus spp. Taht does not require Factor X(Hemin) as its growth factor maybe ide
ntified using which of the following test result?
Microbiology Post-test 2018

-d-ALA (+) /Porphyrin test(+)

59. One of the following is a penicillin sensitive bacterium.
-Bacillus anthracis
60. Disinfection of table surfaces using Lysol will destroy which of teh following microbial form?
-Vegetative forms only
61. This spxn will most likely produce a positive result for M. Tuberculosis.
-All of these (Sputum, Bone biopsy, skin biopsy)
62. This lesion is seen in secondary syphilis.
-maculo-papular lesion
63. One of teh following is responsible for the geriatric type (affecting old people) of pneumonia.
-Legionella pneumophila

64. One of the following is NOT a chlamydial disease.

-Meningitis
65. A small child develops cough and leukocytosis with absolute lymphocytosis. which of the following i
s the most likely disease.
-Pertussis
66. One of the foolowing is used to make a fianl diagnosis of C. Diphtheriae
-Virulence test (ELEK test)
67. Unpasteurized milk is less likely to transmit which of the following disease?
- Q fever
68. A child admitted with gastro-enteritis ad flaccidity of musclesof the leg. An examination of the feces
revealed teh presenof Clostridium botulinum toxin. Which of the following is most likely teh route of en
try?
-ingestion of spores present in honey
69. which of the following describe Gardenrella vaginalis?
-1,2,3 and 4 (Sniff test positive, Gram positive cell wall, genital swab pH >4.5, stains gram neg)
70. Which of teh following best describe Pseudomonas spp.?
-1,2 and 3 (Grows best at 42C, Antibiotic resistance, Production of aldehyde-like odor)
71. Prions are known to cause which of the following diseases?
-All of these (scrapie, CJD, kuru)
72. Which of teh above is the human variant of bovine spongiform encephalopathy (BSE)?
-CJD
73. The Gerstmann-Stradussier-Scheinker syndrome is a familial form of CJD disease.
-True
74. An icosahedral (cubical) symmetry results from the characteristic assembly of the protein subunits(c
apsomeres) of teh capsid. it is composed of the following.
-both (20 triangular faces and 12 vertices)
75. Eboala nd MArburg viruses belong to which of the following groups?
Microbiology Post-test 2018

-Filavirus(filamentous)
76. This virus is ether-resistant.
-Picornavirus
77. One of teh following is the recommended processing methdo employed in teh rpeervation of urine
for the isolation of CMV.
-refrigeration at 2-8C
78. One of the following cell culture is prepared from laryngeal carcinoma
-Hep2 cells
79. Teh MDCK cell culture is used in teh isolation of one of the following.
- Haemophilus influenzae A
80. Guarneri bodies are CPE found in cells infected by one of the following
-variola(small pox)
81. Adult gastroenteritis resulting from eating of contamianted shelfish and water.
-Norwalk-like virus
82. Acute gastroeneteritis of the newborn and young children
-Rotavirus
83. Which of the following viral infxns s associateth arthralgia and skin rashes.
-2 and 4(Chikungunya and Dengue)
84. Which of the following shows tis CPE in teh infected cell: presenc eof large, multinucleated cells “Ow
l’s eye nucleus” inclusion.
-CMV
85. TORCH test- Toxoplasmosis, Rubella,Cytomegalovirus and Herpes simplex
86. One of teh following viruses is not associated with pulmonary infxn
-Flavivirus (dengue and Hepa)
87. The following characterize Hepa D virus
- Its presence indicates Hepa B
88. The hepatitis develops within 24hrs
-Hepa A
89. This virus targets teh endothelial cells
-EBV -- HIV;CD4: HTLV-I; Cytotoxic Tcells: Poxvirus; Epithelial cells
90. A window stage in HIV/AIDS is cahracterized by the following. (The window stage maybe interprete
d as a negative result especially if limited parameters are tested).
-p24-, gp41-,anti-gp41+