LEUKEMIA
Acute Myeloproliferative
(LF Neutropenia, Anemia,
Thrombocytopenia,
hypercellular marrow, 20%
(WHO) or 30% marrow blasts)
AML
M0 (AML minimally diff’d
signs of matn)
30% BM myeloblast
MPO and SBB (+) in 3%
blasts
M1 (AML wo matn)
M6 (Acute Erythroleukemia)
90% marrow myeloblast,
auer rods
M2 (AML w matn)
90% marrow myeloblast,
auer rods, t (8:21)
M3 (APL)
30% marrow
promyeloblast w bundles
of auer rods (Faggot
cells), t (15:17), DIC
MPO, SBB, SE (+)
M4 (AMML)
Aka ¨Naegeli monocytic
leukemia¨
30% marrow myeloblast
w 20% cells of
monocytic origin, auer
rods, M4E: t (16:16),
MPO, SBB, SE, NSE (+)
Chronic Myeloproliferative
M5 (AMoL)
Aka ¨Schilling’s leukemia¨
20% or 30% marrow
monoblasts, auer rods,
NSE (+)
Aka ¨DiGuglielmo’s syndrome¨
20% or 30% marrow
myeloblasts, 50% erythroid
cells, hypercellularity
Strongly PAS (+)
 AB erythroid cell
PAS (-)  Normal erythroid cell
v left, Philadelphia chromosome,
M7 (Acute Megakaryocytic
leukemia)
CD41, CD42 and CD61(+) platelet
marker,
Require immunocytochemical
staining for accurate dx
Only FVIII (+)
Acute Lymphoproliferative
(LF: hypercellular, erythrocytosis,
granulocytosis, thrombocytosis)
Essential thrombocythemia
Proliferation of Megakaryocytes
LF: plt ct 1000x109L, giant plts, plt
fxn abnormality, Leukocytosis
PV
Inc. in all cell lines
Inappropriate erythropoiesis
despite dec. EPO
LF: rbc ct (7-10x1012L),
Hb (20gdL), Hct (60%),
Leukocytosis and Thrombocytosis
CML
LF: wbc ct (50-500x109L), shift to the
t (9:22)
Chronic Idiopathic
myelofibrosis
Progressive stem cell disorder char
by progressive marrow fibrosis,
prolif of erythroid, granulocytic
and megakaryocytic precursor in
the marrow w dyspoesis.
LF: Leukoerythroblastic anemia,
anisocytosis, poikilocytosis with
teardrop
Chronic
(LF: neutropenia, anemia,
thrombocytopenia, lymphoblast-
PAS (+), SBB and MPO(-))
FAB L1
Most common childhood leukemia
LF: small lymphoblast with
Homogenous appearance, tdt (+),
PAS (+)
FAB L2
Common in adults
LF: large lymphoblast with
Heterogenous appearance, tdt (+),
PAS (+)
FAB L3
Burkitt lymphoma
Both adults and children
LF: large lymphoblast with
Heterogenous appearance with
nuclear and cytoplasmic
vacuoles, t (8:14)
Plasma cell neoplasm
Lymphoproliferative
CLL Multiple myeloma
Most common adult leukemia,
Male to female ration2:1 Monoclonal gammopathy, excess
LF: hypercellular, absolute prodxn of IgG by B cell
lymphocytosis 5x109L, LF: BM plasma cell (30%),
homogenous, small
marked rouleaux, inc ESR, blue
hyperclumped lymphocytes and bkgd in PBS, lymphocytes and
Smudge cells plasma cells
Prolymphocytic
rance, tdt (+), PASLeukemia
(+) (PLL) Waldenstrom’s
macroglobulinemia
80% B cell origin, 20% T cell origin
LF: lymphocytosis (100x109L), Monoclonal gammopathy, excess
prodxn of IgG by B cell
anemia and thrombocytopenia
LF: marked rouleaux, inc ESR,
blue bkgd in PBS, plasmacytoid
lymphocytes, lymphocytes and
Hairy cell leukemia (HCL)
plasma cells
Aka ¨Leukemic
Reticuloendotheliosis¨
LF: pancytopenia, lymphocyte
cytoplasm with hair-like
projections, TRAP (+)