BIOCHEMISTRY OSPE 2018

Dr Sahar Awais
FMHC&D
 Unobserved station 1
Marks: 02 Time: 2 minutes

For Candidate
Task:
Carefully answer the following question:

1. Name the most common method used to determine total serum cholesterol in
a clinical chemistry Lab? (1)
2. What is the normal range of plasma triglyceride level? (1)

Unobserved station 1
Marks: 02 Time: 2 minutes

For Examiner
Key:

1. Cholestrol Oxidase-phenol, 4- aminophenazone (CHOD-PAP) method.

2. 150 mg/d L or below

Unobserved station 2
Marks: 02 Time: 2 minutes

For Candidate
Task:
Carefully answer the following question:

Q2. 1. What is ALT and under which clinical conditions its levels are raised? (01)
2. What is the function of ALT? (01)

Unobserved station 2
Marks: 02 Time: 2 minutes

For Examiner

Key:
1. Alanine Aminotransferase. Its levels are raised in myocardial infarction &
hepatitis.
2. It catalyzes the transfer of an amino group from Alanine to alpha
ketoglutarate forming pyruvate & glutamate.

Unobserved station 3
Marks: 02 Time: 2 minutes

For Candidate
Task:
Carefully answer the following question:

Q3. 1. What is the normal range of total plasma protein? (01)

2. What is the condition called when the total protein assay of a patient shows a
value of less than 5g/dl? (01)

Unobserved station 3
Marks: 02 Time: 2 minutes

For Examiner
Key:
1. 5.5 g/dl- 7.5g/dl
2. Hypoproteinemia

Unobserved station 4
Marks: 02 Time: 2 minutes
 For Candidate
Task:
Carefully answer the following question:

Q4. A nine year old diabetic child is brought to the emergency in a semi-
comatose condition. History reveals that he has not taken insulin for the
past six days. His breath has fruity odour.
1. What is the cause of fruity odour? (01)
2. Name two products of acetyl Co A metabolism? (01)

Unobserved station 4
Marks: 02 Time: 2 minutes

For Examiner
Key:
1. Acetone
2. Acetone, Acetoacetate & Beta hydroxybutyrate
 Unobserved station 5
Marks: 02 Time: 2 minutes

For Candidate
Task:
Carefully answer the following question:

Q5 1. Enumerate two causes of hyperuricemia. (01)

2. Which enzyme is deficient in Xanthinuria? (01)

Unobserved station 5
Marks: 02 Time : 2 minutes

For Examiner
Key:

1. Primary Gout
Lesh Nyhan Syndrome
High protein diet
High alcohol intake
Malignancy
2. Xanthine Oxisdase
 Unobserved station 6
Marks: 02 Time: 2 minutes

For Candidate
Task:
Carefully answer the following question:

Q6 1. Enumerate two renal function tests? (01)

2.What is urea clearance? (01)

Unobserved station 6
Marks: 02 Time : 2 minutes

For Examiner
Key:
1.
a. Estimation of urea level in the blood
b. Estimation of creatinine in serum
2. Urea clearance= mg of urea excreted per min/mg urea per ml of plasma
 Unobserved station 7
Marks: 02 Time: 2 minutes

For Candidate
Task:
Carefully answer the following question:

Q71. What is micro-albuminuria? (01)

2. . Name two conditions in which proteinurea occurs? (01)

Unobserved station 7
Marks: 02 Time: 2 minutes

For Examiner
Key:
1.. When small quantity of albumin (50-300 mg/day) is seen in urine.
2.. 1. Nephrotic syndrome
2. Multiple myeloma
3. Acute Glomerulonephritis
4. Pyelonephritis
.
 Unobserved station 8
Marks: 02 Time: 2 minutes

For Candidate
Task:
Carefully answer the following question:

Q8 1. Which tests will be positive in a patient with Obstructive Jaundice? (01

2.Enumerate any two important Liver Function tests? (01)

Unobserved station 8
Marks: 02 Time : 2 minutes

For Examiner
Key:

1.Gemelin’s test, Hay’s test & Fouchet’s test

2. Serum Bilirubin, Alkaline Phosphatase, Serum albumin, urine bile salts &
bile pigments

Observed station 9
Marks: 03 Time: 3 minutes

For Candidate
Task:
Carefully answer the following question:

1. Name the apparatus. (01)

2. Draw and dispense 400 microliter liquid with the given apparatus. (02)

Observed station 9
Marks: 03 Time: 3 minutes

For Examiner

Key:

1.Auto pipette/ Micropipette

2.(0.5 marks each)

 Adjust the auto pipette to zero

 Place the disposable tip on the micropipette
 Adjust the volume of the pipette to 400 micro liter. Push the button on top & place
the pipette in the liquid. Slowly release the button
 Take out the pipette & dispense the liquid in the given test tube.
 Observed station 10
Marks: 03 Time: 3 minutes

For Candidate
Task:
Carefully answer the following question:

Q10. Centrifuge the given sample for one minute at 3000rpm?

Observed station 10
Marks: 03 Time Allowed: 3min

For Examiner

Key:

(0.5 MARKS EACH)

 Student takes two centrifuge tubes.
 Places the sample in one
 Places an equal amount of water in the other centrifuge tube.
 Adjusts the speed at 3000 rpm
 Places the tubes in opposite holders of centrifuge machine
 Switches on the timer of the centrifuge machine