CUSTOMER SAMPLE

QUESTIONNAIRE
Please complete the form, sign and send it per Email/FAX to the given contact.

A FISABIO CONTACT

FISABIO
Servicio de Secuenciación
Avda Catalunya nº 21,
46020 Valencia, Spain
Phone: +34 961925958
Fax:
Email:

B CUSTOMER CONTACT

Name:
Institution:
Address:
Town:
Postal Code:
Country:
Phone:
Fax:
Email:

C GENERAL SAMPLE DESCRIPTION

Please indicate the species which your sample derives from.

Sample origin:

D SAMPLE TYPE

gDNA cDNA
BAC Plasmid
Cosmid/Fosmid Long Range PCR Product

Other (Provide an explanation of content below):

     
E SAMPLE PREPARATION
Please indicate in few keywords the kit/method used for the preparation of your sample.

F SAMPLE INFORMATION
Please fill out the following table with appropriate information.
Samples group columns must be used when different studies are presente in the same assay.
Samples Samples DNA Conc. Total DNA
Volume (µl)
Name Group (ng/ul) (µg)
Sample1
Sample 2
Sample 3
Sample 4
Sample5
Sample 6
Sample 7
Sample 8
Sample 9
Sample 10
Sample 11
Sample12
Sample 13
Sample 14
Sample 15
Sample 16
Sample 17
Sample 18
Sample 19
Sample 20
 1 2 3 4 5 6 7 8 9 10 11 12

G DNA CONCENTRATION MEASURED WITH:

Spectophotometer Picogreen
Nanodrop Other      

Please if possible (only for genomic) provide an image of an EtBr-stained 2% agarose gel
or E-gel containing 1 μL of each sample. Please use a 1 Kb ladder as a reference
standard. Clearly indicate sample identity in each lane.

H SAMPLE REQUIREMENTS
Sample Description Form: A completed Sample Description Form must be included with every
sample.

DNA Quality: DNA must be double stranded and should be purified by column or gel purification
protocols. Samples providing A260/A280 of ~1.8 normally provide optimal results. A number of
commercially available kits will yield DNA of sufficient quality and purity for sequencing. One μL of
each DNA sample should be subjected to electrophoresis on a 2% agarose gel to evaluate for
concentration and quality. A hardcopy image of the EtBr-stained gel should be included with the
sample(s), or sent by email. Please include a 1 kb ladder as a reference and clearly identify the
contents and amount of DNA of each lane.

DNA Quantity: For genomic sequencing projects (high molecular DNA), 1ng of DNA provided at a
concentration greater than 0,2 ng/μL in water is normally adequate if DNA is quantified with
picogreenassay or Qubit. DNA fragments have to be > 0.8 Kb.For other sample types please
contact us for input DNA requirements. For amplicon projects 5ng/ul (10ul total).

If your sample does not fulfil these criteria sequencing might end in suboptimal results!

I SHIPPING INSTRUCTIONS

Sample Submissions: Samples can be provided in water or TE buffer. Individual samples should
be provided in a 1.5 mL microfuge tube. We recommend sealing caps on individual microfuge
tubes with parafilm.

Packaging of Samples: Up to six 1.5 mL sample tubes could be placed into a 50 mL disposable
screwtop centrifuge tube for shipping. We recommend sealing caps on individual microfuge tubes
with parafilm. To prevent the sample tubes from moving during shipping, please pack the tubes
with a paper towel/tissue paper.

Shipping: Samples can be sent at room temperature, on ice or dry ice. Samples should be
shipped via an express (next day) shipping carrier to:

FISABIO
Llúcia Martínez Priego
Servicio de secuenciación
Avda Cataluña nº 21
46020 Valencia, Spain
Phone: +34 961925958

When sample is sent, please send us an email indicating the courier company used and
the reference number of the parcel.