BIOCHEMICAL OXYGEN DEMAND DETERMINATION

BOD Test

The BOD Test is among the most important method in sanitary analysis to determine the polluting power,
or strength of sewage, industrial wastes or polluted water.

It serves as the amount of clean diluting water required for the successful disposal of sewage by dilution.

The sample is filled with in an airtight bottle and incubated at a specific temperature for 5 days (can be 2,
10 or even 20 days). The Dissolved Oxygen (DO) content of the sample is determined before and after
5 days of incubation at 20OC and the BOD is calculated from the difference between initial and final DO.

Apparatus Reagents
 BOD Incubator  Calcium chloride  Potassium hydroxide
 Burette & Stand  Magnesium sulfate  Potassium iodide
 BOD Bottles  Ferric chloride  Sodium azide
 Erlenmeyer Flask  Dipotassium hydrogen  Concentrated Sulfuric acid
 Pipet (w/ elongated tips) phosphate  Starch (indicator)
 Graduated Cylinder  Potassium dihydrogen  Sodium thiosulfate
 Wash Bottle phosphate  Distilled/deionized water
 Disodium hydrogen phosphate
 Ammonium chloride
 Manganous sulfate
Procedure
1.