Experiment No.

3 :-DISSOLVED
OXYGEN
Aim: To determine the dissolved oxygen in the given
water sample by Iodometric method.

Apparatus: BOD bottle, pipette, conical flask, Dropper,

Measuring cylinder.

Reagent: 1. Manganese sulphate solution

2. Alkali iodide azide solution
3. Conc. Sulphuric acid
4. 0.025N sodium thiosulphate solution
5. 2% starch solution
6. water sample
Theory: Determination of dissolved oxygen is important for boiler
feed water as well as in sanitary engineering practice. Dissolved
oxygen causes corrosion in boilers. Dissolved oxygen is needed for
living organisms to maintain their biological processes. DO
(Dissolved Oxygen) is also important in precipitation and
dissolution of inorganic substances in water.
Dissolved oxygen is an important factor in corrosion.
DO(Dissolved Oxygen) test is used to control the amount of oxygen
in boiler feed water by mechanical, physical and chemical
methods. DO (Dissolved Oxygen) is necessary for all aerobic
DO (Dissolved Oxygen) test helps to assess the raw water quality and to
keep a check on stream pollution. DO(Dissolved Oxygen) test is the
basis of BOD (Biological Oxygen Demand) test which is an important
parameter in evaluating the pollution potential of domestic and industrial
wastes.

The principle involved in this method of determination of DO (Dissolved

Oxygen) is to bring about the oxidation of potassium iodide to iodine with
the dissolved oxygen present in the water sample after adding MnSO4,
NaOH & KI.

The basic manganic oxide formed from NaOH & MnSO4 acts as an
oxygen carrier to enable the dissolved oxygen in the molecular form to
take part in the reaction.
The DO(Dissolved Oxygen)present in the sample oxidizes the Mn2+
to its higher valency which precipitates as a brown hydrated oxide
after the addition of NaOH and KI. On acidification, the manganese
reverts back to the divalent state and an equivalent amount of
iodine is liberated from the KI present. This liberated iodine is
treated against standard sodium thiosulphate (hypo) solution using
starch as an indicator.
MnSO4 + 2 KOH Mn(OH) 2 + K2 SO4

2Mn(OH)2 + O2 2 MnO(OH)2
Basic Maganic
oxide

MnO(OH) 2 + MnSO4 + 2H2O +

H 2SO4 O

2 KI + H2SO4 + K2SO4 + H2O +

O I2

I2 + 2 Na 2S 2O3 Na2S 4O6 + 2

Sodium NaI
thiosulphate Sodium tetrathionate
Determination of DO(Dissolved Oxygen) comes under
Iodometric titration because in Iodometric titration iodine is
to be liberated due to chemical reactions within the reaction
mixture and this liberated iodine is titrated against standard
reducing agent this type of titration is called as Iodometric
titration.
If reducing agent is directly titrated with Iodine this type
of titration is called as Iodometric titration.
Procedure:

1. Take 300ml DO bottle & filled completely with sample of water, stopper
immediately to avoid contact with air.
2. Add 2ml MnSO4 & 2ml of Alkali iodide azide solution to it.

3. Mix thoroughly by inverting bottles 4 to 5 times makes the precipitate settle.

4. Add 2ml of conc.H2SO4 re-stopper & de-precipitate by shaking.

5. Now the total volume becomes 300ml.Take 203 ml sample in conical flask for
titration.
6. Add 2 to 4 drops of starch indicator to it & titrate against 0.025N Na2S2O3
solution
7. End point of titration from blue to colorless
Fig: Titration of water sample with (0.025 N) Na2S2O3 solution.
 Observations:
1. Solution in burette: 0.025N Na2S2O3 solution.

2. Solution in pipette: 203 ml sample water.

3. Indicator: starch indicator

4. End point: Blue to colorless

5. C.B.R.: --------ml
Result: The amount of dissolved oxygen in the given water sample is-----------mg/
lit.
THANK YOU