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An. Bras. Dermatol. vol.84 no.6 Rio de Janeiro Nov./Dec. 2009
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Luciane Donida Bartoli MiotI; Hélio Amante MiotII; Márcia Guimarães
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da SilvaIII; Mariângela Esther Alencar MarquesIV
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IPhD in Pathology, Dermatologist of the Department of Dermatology and
Radiation Therapy of Botucatu School of Medicine (Unesp) - Botucatu (SP), Curriculum ScienTI
Brazil
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IIPhD Assistant Professor of the Department of Dermatology and Radiation
Therapy of Botucatu School of Medicine (Unesp) - Botucatu (SP), Brazil Indicators
IIIPhD Assistant Professor of the Department of Pathology of Botucatu School
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IVAdjunct Professor of the Department of Pathology of Botucatu School of Share
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ABSTRACT

Melasma is a common dermatosis that involves changes in normal skin pigmentation, resulting from the
hyperactivity of epidermal melanocytes. The consequent hyperpigmentation is mostly induced by ultraviolet
radiation. Clinically, melasma is characterized by light to dark brown macules that usually occur on the face,
although they can also affect the cervical and anterior thoracic regions and upper members.Fertile age women and
those with intermediate skin phototypes are most likely to develop melasma. Most of its physiopathogenics is not
yet fully understood, but there is a relation with genetic and hormonal factors, drugs and cosmetics use,
endocrinopathies and sun exposure. The authors discuss the main aspects associated with skin pigmentation and
the development of melasma.

Keywords: melanosis; skin pigmentation; ultraviolet rays; pigmentation disorders

INTRODUCTION
Normal skin color

The skin is the most visible aspect of the human phenotype and its color is one of its most variable features. Little
is known about the genetic, evolutional and cultural aspects related to the definition of human skin color
patterns.1,2

We believe that variations in skin color are evolutional and are related to the regulation of the penetration of
ultraviolet radiation (UVR). 3,4

The synthesis of vitamin D on the skin, degradation of folic acid by UVR, resistance to direct sun exposure and
cultural elements are arguments that try to explain the phenotypical distribution of skin color in different latitudes
of the planet.5,6

Normal human skin color is mainly influenced by the production of melanin, a dense high-molecular-weight brown
pigment, that looks darker the more concentrated it is.7-9

However, exogenous yellow pigments, the carotenoids, also contribute to skin color, as well as endogenous red,
oxygenated hemoglobin in capillary vessels in the dermis, and endogenous blue, reduced hemoglobin in venulas.
7,9

In humans, skin and hair pigmentation depends on the melanogenic activity inside melanocytes, melanin synthesis
rate, as well as on the size, number, composition and distribution of cytoplasm particles of the melanocytes called
melanosomes, in addition to the chemical nature of the melanin they have.8-11

Melanocytes and melanosomes have a relatively constant number in different races, as discussed ahead.9

Melanocytes

Melanocytes are phenotypically important cells, responsible for skin and hair pigmentation, contributing skin tone
and providing direct protection against the damages caused by UVR.9

They are dendritic cells, embryologically derived from melanoblasts, which originate from the neural crest,
migrating to the skin shortly after the closing of the neural tube. This migration may occur to many destinations,
and the signalizer that direct this process still need to be better described.8,12

When they become fully developed cells, they spread through different sites: the eyes (retina pigment epithelium,
iris and choroid), ears (vascular strias), central nervous system (leptomeninges), hair matrix, mucosas, and
skin.8,12

On the skin, they are located on the basal layer of the epidermis and, occasionally, on the dermis. They project
their dendrites through the Malpighii stratum where they transfer their melanosomes to keratinocytes (Figure 1).
This melanocyte-keratinocyte association is called epidermal melanin unit and is formed, in humans, by one
melanocyte and about thirty-six keratinocytes.8,13,14
Epidermal basal cells are united to adjacent cells by specific structures called desmosomes and to the basement
membrane by hemidesmosomes. Melanocytes are not fixed on the epidermis; they can only be identified by a small
unevenness in their position in relation to the alignment of the basal layer, projecting slightly towards the dermis
(Figure 2).11

The density of melanocytes varies in different parts of the body. There are about 2,000 or more epidermal
melanocytes per square millimeter of skin in the head and forearm, and about 1,000 in the rest of the body, in all
races. This exact regulation of the number of melanocytes on the epidermis seems to be mediated by keratinocytes
and by specific mediators like the fibroblast growth factor (FGF2).11

The number of melanocytes goes down with age, in areas not exposed to light, at the proportion of 6% to 8% per
decade, and racial differences in pigmentation are not due to a significant variation in the number of melanocytes,
but rather on their level of activity (melanosomes and melanin synthesis), in the proportion of melanin subtypes
(pheomelanin and eumelanin), their distribution, and environmental factors like sun exposure, which directly
stimulates the synthesis of melanin.11,15

The melanin produced in melanocytes is stored in specific intracytoplasmatic structures called melanosomes.

Melanosomes

Melanosomes are highly specialized elliptical organelles, where there is synthesis and deposition of melanin (Figure
3), storage of the tyrosinase synthesized by ribosomes, and they are the site of the biochemical phenomena that
originate melanin.7
Melanin synthesis takes place exclusively in melanosomes and dependents on many genes.

Melanosomes develop in a series of morphologically defined stages (Figure 4), from unpigmented structures (stage
I) to striped organelles filled with melanin (stage IV).11,16

The fundamental phenotypical difference between the more pigmented and less pigmented races does not reside
on the production of melanin, neither on the number of melanocytes, but mainly in the quality of the melanosomes
(Table 1)16.

Melanosomes are larger and more mature in black people than in white people and are stored more as units than
in clusters. The degradation of larger melanosomes in keratinocytes is delayed, which also contributes to the higher
levels of skin pigmentation in these cases.8 The processes that lead to this behavior difference need to be further
clarified.

In normal skin melanosomes, melanin is extremely dense. It is an insoluble high-molecular-weight nitrogenated

polymer that forms a pigment, which, in addition to providing color to the skin, plays a protection role, filtering and
absorbing UVR. It plays, therefore, an important photoprotective role against UVR damages, as shown by the
inverse correlation between the content of melanin on human skin and the incidence of skin carcinomas and
melanomas.11,17

Melanin

Melanin is the main biological pigment involved in skin pigmentation, and is determinant for the differences in skin
color.11

The initial element of melanin biosynthesis is tyrosine, an essential amino acid. Tyrosine suffers the chemical action
of tyrosinase, a copper protein enzyme complex synthesized in the ribosomes and transferred through the
endoplasmic reticulum to the Golgi apparatus, and it is clustered in units surrounded by a membrane, i.e. the
melanosomes.11

The three members of the tyrosinase-related family (tyrosinase, Tyrp 1 or tyrosinase-related protein 1, and Dct or
dopachrome tautomerase) are involved in the melanogenesis process leading to the production of either eumelanin
(brown-black) or pheomelanin (yellow-red).18

In the presence of molecular oxygen, tyrosinase oxidizes tyrosine into dopa (dihydroxyphenylalanine) and this into
dopaquinone. From then on, the presence or absence of cysteine determines the course of the reaction for the
synthesis of eumelanin or pheomelanin.19

In the absence of cysteine (glutatione), dopaquinone is converted into cyclodopa (leukodopachrom) and this into
dopachrome. There are two degradation pathways for dopachrome, one that forms DHI (dopa,5,6 dihydroxyindole)
at a larger proportion and another that forms a smaller quantity of DHICA (5,6 dihydroxyindole-2-carboxylic acid).
This process is catalyzed by dopachrome tautomerase (Tyrp 2-Dct). Finally, these dihydroxyindoles are oxidized
into melanin.19

Tyrosinase related protein 1 (Tyrp 1) seems to be involved in the catalysis of the oxidation of DHICA into
eumelanin. On the other hand, in the presence of cysteine, dopaquinone rapidly reacts with this substance to
generate 5-S-cysteinyldopa and a smaller proportion of 2-S-cysteinyldopa. Cysteinyldopas are then oxidized into
benzothiazine intermediates and finally produce pheomelanin (Figure 5).19

Eumelanin is an insoluble brown alkaline polymer, and pheomelanin is a soluble yellowish alkaline pigment.
Pigments similar to pheomelanin, however, can be structurally derived from eumelanin, as it can be oxidized in the
presence of metallic ions, resulting in a lighter soluble pigment. Another sulfur-containing pigment, derived from
pheomelanin, which can be found in small quantities in red human hair, and is called trichrome.13

In this manner, melanogenesis has three different and important steps. The first step is the production of
cysteinyldopa, which continues as intense as the quantity of cysteine present. The second step is the oxidation of
cysteinyldopa to form pheomelanin, a process that depends on the quantity of cysteinyldopa present. And the third
and last step is the production of eumelanin, which only starts after the depletion of most of the cysteinyldopa.
However, eumelanin seems to deposit on pre-formed pheomelanin and the ratio between pheomelanin and
eumelanin is determined by the activity of tyrosinase and availability of cysteine.19

Eumelanin absorbs and disperses ultraviolet light, attenuating its penetration on the skin and reducing the harmful
effects of the sun. In other words, people with more pigmentation tend to get less sun burnt and to tan more than
those who are lighter skinned.15,20,21

Pheomelanin, on the other hand, has a great potential to generate free radicals in response to UVR, which are
capable of causing damage to DNA, and, in this manner, may contribute to the phototoxic effects of UVR. This
explains why people with light skin, who have relatively high quantities of pheomelanin, are at greater risk of
ultraviolet-induced epidermal damage, including neoplasms. 20

Individual melanocytes typically synthesize eumelanins and pheomelanins, and their rate is determined by a
balance of variables, including the expression of pigment enzymes and the availability of tyrosinase and specific
reducing agents in the cell.9

Melanocortin 1 receptor (MC1-R) controls the amount of eumelanin and pheomelanin inside melanosomes. This
proportion is an important determinant of sun sensitivity in humans. However, probably the total quantity of
melanin produced is even more important than the ratio of the types of melanin.5 It is known that melanocytes
derived from intensely pigmented skin present greater quantity of total melanin and also a greater rate of
eumelanin than the melanocytes derived from light skins.10

Total skin melanin is the result of a combination of monomers of pheomelanin and eumelanin and the proportion
between the two determines the final phenotypical expression of skin and hair color.

A reduction in eumelanin and the predominant presence of pheomelanin, as in red-haired people, are regaled
chiefly by MC1-R.11
Two types of melanin pigmentation are the basis for normal skin color. Constitutive skin color is the genetically
determined color of healthy skin not exposed to UVR and it plays a key role in photoprotection against the UVR that
reaches the Earth, thus minimizing the damages to DNA that may lead to the emergence of skin cancer.7,17

Facultative skin color is the more intense skin color resulting from sun exposure or pigmentary diseases and
reflects the genetically determined tanning capacity in response to UVR. In this manner, the "tanning" level is
genetically determined and is the basis for the division of normal skin in adaptive response patterns called
phototypes.7

After the complete synthesis of melanin, melanosomes filled with this pigment are injected in the interior of
keratinocytes in the corresponding epidermal melanin unit through the dendritic extensions of melanocytes (cytocin
activity). Once inside keratinocytes, melanosomes tend to spread through the cytoplasm, over the upper part of
the nucleus, so as to protect it from ultraviolet radiations. It has been suggested that the pigment inside these cells
also works as a scanner of photoproduced free radicals, always striving to protect cell DNA.12,22,23

The photoprotection properties of the melanin of human skin have been well documented and take place through
the absorption and dispersion both of ultraviolet light and visible light. This absorption increases linearly at the
range of 720-620 nm and then exponentially through shorter waves (300-600 nm). 17

Melanin has great affinity for DNA, and is capable of producing reactive oxygen species in response to ultraviolet
radiation A. In people with light skin, the greater incidence of melanomas seems to result not only from poor
natural protection, but also from increased mutations promoting the formation of pheomelanin and/or melanin
intermediates.8,11,24

Ultra-structural studies revealed that eumelanosomes, which are usually produced by dark skins, remain intact on
the epidermis after exposure to UVR, whereas in light skin, no intact melanosome can be detected after this
radiation. 17

The main factors that regulate the quantity and quality of the melanin produced by melanocytes include UVR, -MSH
( -type melanocyte stimulating hormone or melanocortin), ASP (AGOUTI signaling protein), and MC1-R.8,25

α-MSH and MC1-R

The melanin pigmentation of human skin suffers intense hormonal control. In 1967, Snell summarized the
prevailing consensus about hormonal action on the melanocytes of mammals, particularly humans.26,27

Injections of α-MSH and β-MSH in human individuals produced a darkening of the skin resulting from the high
melanogenesis inside epidermal melanocytes and increased transportation of melanosomes derived from
melanocytes, to keratinocytes, without the need of exposure to UVR. Hyperpigmentation of the skin is also seen
when human individuals were injected with high doses of adrenocorticotropic hormone (ACTH).11,26,28,29

α-MSH is a tridecapeptide with a sequence identical to the first 13 amino acids of ACTH. The proteolytic cleavage of
proopiomelanocortin (POMC), on the pituitary gland, is responsible for the origin of many byproducts, including α-
MSH. It is also known that POMC is expressed and suffers cleavage in other places, including the brain and skin. α-
MSH was the first of the peptides derived from POMC to be identified on the skin.20

Human keratinocytes are capable of synthesizing α-MSH and β-MSH at physiological quantities. α-MSH is also
produced in melanocytes and Langerhans cells.9,11,17,25,30-34 Evidences indicate that these hormones play a
paracrine role in the regulation of the functions of melanocytes. More than 120 genes have been identified and
seem to regulate pigmentation; however, the effects of -MSH are mediated by MC1-R, which is expressed on the
surface of melanocytes, and is considered the key point for pigmentation. It is also present in other cells like
monocytes, neutrophiles, glioma cells, astrocytes, macrophages, fibroblasts, endothelial cells, and keratinocytes.
As MC1-R has a wide tissue distribution, it probably is associated to a large number of biological
functions.9,11,17,25,30,32-35

In 1992, Mountjoy et al.36 from the Oregon University of Health Sciences, in Portland, reported they had cloned an
MC1-R hormone receptor, in humans and rats. They also demonstrated that mutations in the gene of this receptor
caused hair color changes in rats. In 1995, Valverde et al.37 reported a similar association between abnormal forms
of the receptor and variations in the skin and hair color of people.

In spite of the identification of more than 100 loci involved in the pigmentation of vertebrates, MC1-R is the chief
determinant of the pigmentation phenotype. The extension of its locus was first identified in rats based on hair
color changes. Recessive mutants had yellowish or pheomelanotic hair, while wild-type rats had dark/brown or
eumelanotic hair.9

In the early 90's, molecular structure of the MSH receptor was described; it was then called MC1-R and its
antagonist, AGOUTI signaling protein (ASP). For many years, two loci were known to be involved in the qualitative
regulation (eumelanin and pheomelanin) and quantitative regulation of the pigmentation of mammals, and ASP
was produced in the follicles and acting in follicular melanocytes through the inhibition of the synthesis of
eumelanin.17,38,39 Before cloning, two melanocortin receptors, MSH and ACTH receptors, were discovered in
classical pharmacological and physiological studies.

The melanocortin system consists of peptides with many forms of MSH (alpha, beta and gamma) and ACTH; a
family with five melanocortin receptors, linked to protein G was described, with seven transmembrane domains
(seven passages through the membrane) and ASP.17,38,39

MC1-R was the first ?-MSH receptor that was cloned and was isolated from a melanoma cell strain.34 Human MC1-
R gene is located in chromosome 16q24.3 and has a reading frame of 951 base pairs that codify a protein with 317
amino acids. The human protein sequence shows all the characteristics of the receptors coupled to protein G,
including the presence of seven transmembrane fragments and two sites of potential N-glicosilation. The
occurrence of specific high-affinity binding sites in most of the human melanocytes was already known, even
before the MC1-R gene was cloned.25,40,41 However, the number of binding sites is variable, and it can be as low
as a few hundreds per cell, as detected by Scatchard's analysis using radiomarked probes. Not just α-MSH, but also
ACTH, β and γ MSH34 bind to MC1-R.

It is then a highly polymorphic white gene population and these gene variations are associated with light skin and
reddish hair and act in the reduction of the ability of the epidermis to respond to UVR.

Today, the MC1-R gene is considered one of the main markers of susceptibility to malignant skin neoplasms, as
gene variants are associated to an increased risk of melanoma and non-melanoma skin cancers.17,30,34,42-44

Other studies demonstrated that solar ephelis and lentigines are different types of pigmented lesions that present
significant differences in their etiologies, but gene variants of MC1-R are a necessary factor for the development of
ephelis, while they play a less important role in the case of lentigines.3,30,45,46

Variations related to gene MC1-R are exceptionally high among Caucasians and have a significant impact on the
pigment phenotype of this ethnic group. Red hair has been related to some alleles, but recent studies indicate that
the same genotypes may express different hair colors depending on the population. 3,44

MC1-R is abundantly expressed in human and rat melanoma cells, and at significantly lower levels in rat
melanocytes. More recently, it has been demonstrated in normal glands of human skin and hair follicles, as well as
in skin malformations and neoplasms.34

In most individuals with light skin, who do not tan, there is a variation in the gene sequence of classical MC1-R,
which normally determines dark hair and ease to tan.11 In individuals with reddish hair and light skin that have a
predominance of pheomelanin in the hair and skin and/or reduced ability to synthesize eumelanin, a functional
reduction of MC1-R with a resulting decrease in the melanotropin-induced tyrosinase activity, associated with
eumelanogenesis, can be the key in the promotion of pheomelanin synthesis inside human melanocytes.

In this manner, the MC-1R of melanocytes is undoubtedly an important element in the regulation of pigmentation in
mammals, but also one of the most polymorphic.11,20,30,32-34,47 Variant gene sequences are found in more than
80% of people with red hair and light skin, in less than 20% of individuals with brown or black hair and in less than
4% of those who respond easily to tanning. 34

α-MSH signals through MC1-R, activating adenyl cyclase (AC) and increasing intracellular cyclic adenosin
monophosphate (AMPc), resulting in the production of dark eumelanin pigment (Figure 6). Whether MC1-R is
involved in other signaling pathways, still remains unknown, but the activation of MC1-R influences the relative
quantities of pheomelanin and eumelanin produced, and its activity loss is associated to red or yellow
hair.20,34,38,39,48-52
Variants of MC1-R have been associated with red hair inheritance, in which more yellow-reddish pheomelanin
pigment is produced and they present very small tanning capacity. MC1-R variants R160W, R151C, D294H, R142H,
86insA, and 537insC are the main determinants of the red hair and light skin phenotype. It is a phenotype typical
of phototypes I and II, with greater chance of sun burn and development of skin neoplasms.10,20,34

The recently cloned murine gene AGOUTI is located in chromosome 2 and codes protein ASP formed by 131 amino
acids and acts as a competitive antagonist of MC1-R, blocking its activation by α-MSH. However, the shift between
eumelanogenesis and pheomelanogenesis involves the opposition to ASP and α-MSH effects as binders for MC1-R.
Pheomelanogenesis can be stimulated by in vitro treatment with purified recombinant ASP. After treatment with
ASP, the expression of genes that codify tyrosinase and other melanogenetic proteins is suppressed in
melanocytes, which requires other physiological factors typical of in vivo pheomelanogenesis. In normal human
melanocytes, where the number of expressed MC1-R is relatively low, ASP completely annuls α-MSH stimulating
effects in melanocytic and melanogenesis proliferation.17,25,53

The incapacity to tan of individuals with variation in MC1-R is consistent with the critical role MSH/AMPc play in this
response, but some studies indicate that damages to melanocyte DNA may mediate pigmentation induced by
UVR.54

UVR and Pigmentation

The solar radiation spectrum is broad, ranging from cosmic rays (ultra X rays) to infrared radiation. Shorter
wavelength radiations, up to 200 nm, do not reach the Earth, because they are absorbed by atmospheric oxygen
and ozone.17,55 UVR and visible light are between 200 nm and 760 nm and form the photobiological spectrum,
with ultraviolet between 200 and 400 nm and visible light between 400 and 760 nm. Beyond this limit, up to 17000
nm is infrared, which is a heat inducer.17,55

The acute effects of exposure to UVR are basically two: skin burn and/or tanning. The individual response to
exposure to UVR, that is, how tanned one may get, is one of the best examples of human adaptation to the
environment. 9

After one single exposure to UVR, an increase in the size of melanocytes can be observed, followed by an increase
in tyrosinase activity. Repeated exposures to UVR lead to an increase in the number of stage IV melanosomes
transferred to keratinocytes, as well as an increase in the number of active melanocytes. Moreover, the density of
melanocytes, in comparative studies, is greater in photoexposed areas.16

Therefore, UVR is an efficient stimulant of skin pigmentation in humans and is responsible for the initiation of the
tanning response. Many mechanisms may be involved and the response is believed to be the result of a
combination of different signals acting both directly and indirectly in melanocytes. Indirect UVR action involves the
release of keratinocyte mediators in the skin.33,40

Ultraviolet-B radiation (UVB) on human skin induces the production of α-MSH and ACTH in melanocytes and
keratinocytes. α-MSH stimulates the activity of tyrosinase and in vivo melanin synthesis, and the synthesis of
melanocytes through MC1-R. Other reports indicate that the irradiation of melanocytes with UVR increases MC1-R
RNAm levels. Moreover, the synthesis of many epidermal factors, including α-MSH, ACTH and endothelin-1, is
increased by the exposure to UVR, suggesting an important influence of these mediators on the response of
melanocytes to sun light.32,56-58

Ultraviolet C (UVC) (200-290 nm) is basically germicide; UVB (290-320 nm) causes erythema, pigmentation and
especially alterations that induce skin cancer; and ultraviolet A (UVA) (320-400 nm) penetrates deeper in the skin,
causes pigmentation and cancerigenous alterations, and is the main inducer de photosensitivity. 55

UVB is the chief cause of sun burn, causing erythema after a latency period of 2 to 7 hours. UVA causes erythema
that appears later and may gradually become more intense.55

The interaction of hormones and UVR can be illustrated in melasma. UVR stimulates the production of melanocortin
inside melanocytes and keratinocytes, which explains the involvement of this hormone in the pathogenesis of
melasma that is basically characterized by increased epidermal melanization in melanocytic proliferation.11

Melasma

Melasma is a common, acquired and symmetrical hypermelanosis characterized by more or less dark brownish
maculae, with irregular contour, but clear limits, on photoexposed areas, especially the face, forehead, temples,
and more rarely on the nose, eyelids, chin, and upper limbs (Figure 7).59-62

It is a dermatological disease easily diagnosed by clinical examination, typically chronic, with frequent recurrences,
great refractoriness to existing treatments, and with many unknown physiopathological aspects.57

The word melasma comes from the Greek melas that means black. Chloasma is a term used with the same
meaning, also derived from the Greek cloazein, meaning greenish. The word melasma is, therefore, the most
appropriate designation for the condition.59

Although it may affect both sexes and all races, it is more often in intermediate phototypes and people of Asian or
Hispanic origin that live in tropical areas. It is more common in adult women in childbearing age, but its onset can
also be after menopause. The age of onset is usually between 30-55 years and men account for only 10% of
cases.7,57,61,63,64

Even though melasma is more frequent among Hispanics, its exact prevalence is unknown. Approximately 66% of
Mexican women develop melasma during pregnancy and one third of these women keep the pigmentation for the
rest of their lives.65-67

To provide an idea of this disease, according to a 2000 census in the United States, Hispanics accounted for 12.6%
of the population and this number is estimated to grow annually to 15.5% in 2010 and 24.4% in 2050. 65,67

There is no consensus as to the clinical classification of melasma. Two patterns of facial melasma are recognized:
central-facial, which affects the central region of the forehead, mouth, lips, supra labial area, and chin; and malar,
which affects the zygomatic region. Some authors also add a third and less frequent pattern, called mandibular.
Ponzio & Cruz observed in a study, that 78.7% of the melasmas were central and 21.3% were peripheral.59,63,68

There are countless factors involved in the etiology of the disease, but none of them can be mentioned as the only
factor leading to its development. They include: genetic influences, exposure to UVR, pregnancy, hormone therapy,
cosmetics, phototoxic drugs, endocrinopathies, emotional factors, anti-convulsive drugs, and others with historic
value. However, it seems that genetic predisposition and exposure to sun radiation play an important role,
considering that melasma lesions are more evident during or shortly after periods of exposure to the
sun.7,60,62,63,68-70

Sacre et al., investigating idiopathic melasma, concluded that thyrotropin, prolactin, and gonadotrophin reserves
are normal and that, as ovary and thyroid functions were also normal, it was not possible to establish a correlation
between hormone levels and this form of melasma.71

In contrast with what happens in pregnancy, melasma induced by anovulatory drugs does not regress with the
suspension of the drug and, among the patients that had melasma because of birth control pills, 87% had also had
it in previous pregnancies.63

Genetic predisposition has been suggested by reports of family occurrence. A racial factor has been reported due to
the frequent occurrence of melasma Hispanic patients. Sanchez et al. Identified family history in more than 20% of
the cases studied, and all patients reported exacerbation due to sun light and the use of cosmetics.59,72

It is worth highlighting that melasma is one of the unaesthetic dermatoses that lead to great demand for
specialized dermatological care, even though they are just a common and benign pigmentation abnormality. This
might be explained by its cosmetically compromising nature and the associated emotional and psychological effects
in individuals affected by this problem, who often, because of dissatisfaction with their appearance, eventually
reduce their social lives, even with cases of suicide reported.65-67,69

Although this condition often has only aesthetical implications, such concern can be very important and impacting
on the social, family and professional lives those affected, causing psychological effects that cannot be
neglected.65,73

In 2003, Melasqol, a new tool for assessing health-related quality of life in women with melasma, was published by
Balkrishnan et al.. This instrument was validated and demonstrated utility to monitor the impact caused by
melasma on the quality of life of patients. The main quality of life domains that showed to be affected by melasma
were social life, recreation, leisure, and emotional well being. In 2006, this tool was translated into Portuguese and
culturally adapted, according to the rules defined by the World Health Organization.65-67,74

The treatment of melasma is usually dissatisfactory due to the great recurrence of lesions and due to the absence
of a definitive whitening alternative. Controlled clinical trials indicate photoprotection and the use of whiteners as
the first line elements of its treatment.75-77 However, the discussion about the many treatment modalities, in spite
of the great clinical and academic interest, is outside the scope of this paper.

This being the case, contributing to the understanding of the mechanism through which melanocytes promote
localized phenotypical coloration or how skin color is genetically pre-determined and changes color induced by
factors like sun light, hormones, inflammations, and others, is a task of major importance, and such clarifications
may provide relevant support to treatment innovations and, consequently, improve the quality of life of patients.

Jointly, comparative studies on skin affected by melasma and normal adjacent skin found that this condition is
characterized by epidermal hyperpigmentation without increase in the number of melanocytes, increase in the
quantity of melanin in all layers of the epidermis, increase in the number of melanosomes, and augmented dermal
elastosis.57,78 Dermal pigmentation does not differ on the epidermis with melasma and on healthy adjacent skin,
this finding goes against the classification of melasmas in epidermal, dermal and combined, as proposed by
Wood.2,57,59,63

Recent studies indicate that countless peptides play an autocrine or parocrine regulation in human skin
melanocytes and in many pigmentary diseases. They are represented mainly by: endothelin 1 (ET-1), granulocyte
macrophage colony-stimulating factor, and membrane-bound stem cell factor (SCF). Growth related oncogene- is
also known to regulate the interactions between melanocytes and keratinocytes, hepatocyte growth factor, and
soluble stem cell factor for interactions between fibroblasts and melanocytes.1,79,80

This inter-relation also involves some specific receptors expressed in melanocytes, like endothelin-B receptor, stem
cell factor receptor, and c-KIT. Up or downregulation of this interconnected network is intrinsically involved in the
stimulation melanocytic functions in many epidermal disorders that evolve with pigmentation alterations.79,80

Immunohistochemical findings suggest that a strong immunoreactivity to α-MSH on skin with melasma is one of
the leading factors in the genesis of this disease. The relationship between the photoexposed area and enhanced
immunoreactivity to α-MSH on affected skin has not yet been clarified. However, the existence of a yet unknown
signaling pathway, with increased expression of MC1-R, which may play a significant role in this greater
immunoreactivity to α-MSH should be investigated. There are evidences that a strong expression of α-MSH antigen
in the keratinocytes of the skin affected with melasma, suggesting that α-MSH plays a key role in the
hyperpigmentation of skin with melasma.57,81

In this manner, the evaluation of the expression of α-MSH and MC1-R in the epidermis with melasma lesions,
compared to healthy perilesional skin, would permit estimating the role of MC1-R pathway in the
physiopathogenesis of the disease.

Moreover, β-estradiol increases the expression of α-MSH and MC1-R in melanocytes.51 In addition to that, a recent
study demonstrated an increased expression of estrogen receptors on skin with melasma, as compared to normal
skin, but only two patients were qualitatively evaluated, which does yet not permit determining the real function of
this receptor and of estrogen in the physiopathogenesis of melasma.82

FINAL CONSIDERATIONS
Melasma is a frequent disease in the general population that causes great impact in the quality of life of patients
and drives great efforts to clinical and pharmaceutical research for the development of treatments. However, the
knowledge of its physiopathogenesis is still very limited.65,83

The investigation of estrogen receptors on the epidermis and in melanocytes of healthy and affected skins would
enlighten the role sexual steroid hormones play in the process of localized hyperpigmentation of this disease. 84-88

Research on allele variants of MC1-R that express differently in healthy and affected skin could explain the more
effective pigmentation in certain photoexposed areas than in others.30,46,89

The culture of keratinocytes and melanocytes from healthy and melasma skin and of populations not affected by
the disease, under different exposures, would permit a comparative study of the expression of many genes to
demonstrate the bases of the different phenotypical behavior of these groups of adjacent cells on the same
tissue.90,91

Clinical experimentation with AGOUTI proteins in melasma lesions that compete with α-MSH in MC1-R receptors,
could provide physiopathological grounds for the understanding of the role of -MSH/MC1-R system in the
physiopathogenesis of the disease.79,92

Profiles of melanogenetic cytokines expressed in healthy and affected skins, as well as their cells of origin, local
consequences, and triggering stimulus would provide the understanding of the elements involved in the genesis of
melasma.79,80,93,94

Finally, population-based epidemiological studies or with subgroups of patients with melasma, like pregnant
women, post-menopausal women or men would also contribute to the design of new hypotheses for the natural
history and physiopathogenesis of melasma.

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Mailing Address:
Luciane Donida Bartoli Miot
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