Gram-positive Cocci

Gram-positive Cocci
Catalase Test Catalase Negative
Flow Chart
+ –
Micrococcaceae Streptococcaceae

Hemolysis

Beta β Alpha α
GpC Strep Enterococcus
GpA Strep (S. pyogenes) Strep mitis

Bacitracin Bile Esculin Test

Sensitivity

+ – + –
GpA Strep (S. pyogenes) GpC Strep Enterococcus Strep mitis
Strep pneumoniae

Optichin
Sensitivity

+ –
Strep pneumoniae Strep mitis
 Gram-positive Cocci

Gram-positive Cocci Catalase Test

Catalase Positive
Flow Chart
+ –
Micrococcaceae Streptococcaceae

Coagulase Test Mannitol Hemolysis

Fermentation Test

+ –
Staphylococcus epidermidis
Staphylococcus aureus
Micrococcus luteus
Micrococcus roseus
+ –
Staphylococcus epidermidis
Beta β Alpha α
Staphylococcus aureus Staphylococcus aureus Staphylococcus epidermidis
Micrococcus luteus Micrococcus luteus
Micrococcus roseus Micrococcus roseus

Growth on Pigmentation Growth on MSA

Mannitol Salt Agar Observation or
Test Pigmentation
(MSA) Observation

+ – Grey/White Golden/White Mustard Yellow Pale Pink/Rose

Staphylococcus aureus Micrococcus luteus Staphylococcus epidermidis Staphylococcus aureus
Staphylococcus epidermidis
Micrococcus roseus Micrococcus Micrococcus
luteus roseus

See Pigmentation
Observation to
differentiate
 Gram-negative Rods
Gram-negative Rods
Lactose Positive
Flow Chart Lactose Test
(KIA or Carbohydrate Fermentation Test)

+ _

IMViC Tests

++-- --++
E. coli

Motility

+ -
Enterobacter aerogenes Klebsiella
 Gram-negative Rods Gram-negative Rods
Lactose Negative
Lactose Test
(KIA or Carbohydrate Fermentation Test)
Flow Chart

+ _

Oxidase Test

+ _

Gelatin Test H2S (KIA) Test

+ - + -
Pseudomonas aeruginosa Alcaligenes faecalis Shigella flexneri

Urea Test

+ -
Proteus vulgaris Salmonella typhimurium