Ph.D.

in Chemistry

Antonio Rosato
+39/3207945773 antonio.rosato87@gmail.com antonio.rosato87

WORK / LABORATORY EXPERIENCE

Present R&D and GMP Analytical Chemist
May 2017 Eurofins Biolab
ADDRESS
LC-MS, ICP-MS, ICP-OES analysis of Extractables and
Via Antonio Bazzini, 29 Leachables
20131 Milan (Italy) Method Developement Oral Communications and Validation

- Structural Stability and Ufolding Properties of Atox1 and Mnk1 and their Interaction with Silver Nanoparticles. COST WG meeting Metallodrugs III, Bari (ITALY), 26-27/10/
Quality Control
- Isolation and Characterisation of Monomeric and Dimeric Atox1 Adducts in Aqueous Solution. COST WG meeting Metallodrugs III, Bari (ITALY), 26-27/10/2015
- Uravelling Ag Toxicity Mechanisms: Cu+ Teansport Proteins Interacting with AgNPs and Ag+ ions. Biomet16, Messina (ITALY), 28-29/10/ 2016

Tutoring for the course of General and Inorganic

2015 Chemistry Poster Communications
2016 Università
- Cisplatin Effect on Copper-Dependent degliAtox1Studi
Interaction Between and Mnk1: di Bari,
a Deep Structural Dept.
and Functionalof Pharmacy
Study. A. Lasorsa, A. Rosato, A. Galliani, M. Losacco, G. Nat
Modena (ITALY), 5-7/09/2016

ABOUT ME - NMR Studies on Copper Transport Proteins Interacting with Silver Nanoparticles. M. I. Nardella, V. Mangini, A. Rosato, G. Cavallo, A. Lasorsa, G. Natile, F. Arnesano, XLV GID
2014 Tutoring for the course of General Chemistry and
Positive, motivated and curious Ph.D. 2015 Introductory Biochemistry
- Unravelling Ag Toxicity Mechanisms: Cu+ Transport Proteins Interacting with AgNPs and Ag+ Ions. A. Rosato, V. Mangini, G. Cavallo, A. Lasorsa, M. I. Nardella, G. Natile, F.
Metals in Medicine, Gordon Research Conference: The Interplay of Creative Chemistry and Cellular Processes in Metals in Medicine, Hanover (NH, USA) 26/06 - 01/07 2016
in Chemistry with 3 years of experience - Spectroscopic ApproachesUniversità degli
for Unravelling Ag Toxicity Studi di Bari, Dept. of Medicine
Mechanisms:
in NMR spectroscopic Analysis of Effect of AgNPs and Ag+ Ions on Cu+ Transport Proteins. A. Rosato, V. Mangini, G. Cavallo, A. Lasorsa, M. I. Nardella, G. Natile, F. Arnesano.
Workshop on frontiers of recombinant multi-protein complex expression in insect and mammalian cells, Harwell (Oxford, UK), 13-18/06/2016
Protein-Substrate, and 2 years of - Structural Stability and Unfolding Properties of Atox1 and Mnk1 and their Interaction with Silver Nanoparticles. A. Rosato, A. Lasorsa, V. Mangini, G. Natile, F. Arnesano. S
4/12/2015
experience in LC-MS, ICP-OES and
- Structural Stability and Unfolding Properties of Atox1 and Mnk1 and Their Interaction with Silver Nanoparticles. A. Rosato, A. Lasorsa, V. Mangini, G. Natile, F. Arnesano.
ICP-MS analysis of Extractables and
Leachables. Perform safe laboratory
EDUCATION / LABORATORY EXPERIENCE
Bari (ITALY), 23-24/10/2015
- Proteomic Characterization of Cluster Spots Down Regulated in Parkinson Disease. A. Rosato, C. Pacelli, A. Ferretta, P. Tanzarella, T. Cocco, S. Scacco, F. Palmisano, D. C. Cal
Organization World Congress, Madrid (SPAIN), 5-8/10/2014
practices and GMPs while maintaining Ph.D. in Chemistry
the highest scientific ethical standards.
Apr-(Grosseto),
2017
An Integrated Approach for the Characterization of the Atox1-Dna Complex. A. Rosato, A. Galliani, M. Losacco, F. Arnesano, G. Natile; EMBO Workshop on Magnetic resonan

Università
ITALY, 1–6/06/2014
degli Studi di Bari
Excellent interpersonal, and written Jan 2014
Thesis: Interaction of Platinum Drugs and Silver Nanoparticles
communication skills. Italian citizen with Copper Transport Training
Systems
- Workshop on frontiers of recombinant multi-protein complex expression in insect and mammalian cells, Harwell (Oxford, UK), 13-
and lifelong learner with a global 18/06/2016
research perspective. Solution - State NMR spectroscopy, HPLC-ESI-Q-ToF-MS and
- School of Nanomedicine 2015, CNR-IC Bari(ITALY), 2-4/12/2015

optical spectroscopies Analysis of Proteins

- COST WG meeting Metallodrugs III, Bari (ITALY), 26-27/10/2015
- EMBO Workshop on Magnetic resonance for cellular structural biology, Principina Terra (Grosseto), ITALY, 1–6/06/2014
Method
- Course of Computational Biochemistry, Developement
Dept. of Chemistry, forMoro",
University of Bari "Aldo Protein
Bari (Italy),Expression
3-12/07/2013

- Training in “Proteomic Techniques and Tandem Mass Spectrometry”, Proteomic and Bio-Molecular Mass Spectrometry Centre, ISA (CNR), Avellino (Italy), 10-13/12/2013
Jul 2013 MS in Chemistry
SKILLS Dec 2009 Università degli Studi
Technical Skills di Bari
Thesis in Clinical Proteomics
- Setup of ICP-MS experiments

MS Excel (Customizing Level) - Setup of LC-MS experiments and identification of compounds by MS spectra
MALDI-ToF MS state of art proteomic Analysis
- Acquisition, processing and analysis of NMR spectra for determination of protein interactions

2D Electrophoresis
- In-Cell NMR applied to bacterial cells (E.Coli) and human cells (Hela)
- Eukaryotic (Hela, Human Keratynocytes and Human Fibroblast) cell culture
Masshunter (Customizing Level) Cell Culture
- Prokaryotic (E.Coli) cell culture, transformation of E.coli strains with recombinant plasmids for protein expression
BS in Chemistry
Dec- Acquisition
2009of circular dichroism, dynamic light scattering, fluorescence and absorption spectra for protein structure analysis
- Use of ICP-MS and AtomicUniversità degli Studi disamples
Bari
GMPs Sep- Use2005
Absorption Spectroscopy to quantify metals in biological

Thesis in Organic Chemistry

of ESI-MS to characterize protein-ligand interactions

- Use of MALDI-ToF-MS to characterise protein digests for proteomic studies

Team Work - Recombinant expression and purification by affinity, ion exchange and size exclusion chromatography of uniformly labeled 15 N, 13 C and
13
selectively C-methyonine labelled proteins from E. coli cells

- Biochemistry: SDS-PAGE, Western Blotting, EMSA assays, Agarose Gels for protein and nucleic acid analysis

- Use of Glove Box for oxigen sensitive protein handling

Read English HOBBIES Music (Pianist) –Travelling - Sport

Personal Skills
Piano playing (5th year diploma at Conservatory of music N.Piccinni, Bari-Italy)
sport (body building, jogging)
Additional information
Written English In compliance with the GDPR and the Italian Legislative Decree no. 196 dated 30/06/2003, I hereby authorize you to use and process my
personal details contained in this document

Autorizzo il trattamento dei miei dati personali ai sensi dell’art. 13 d. lgs. 30 giugno 2003 n°196 – “Codice in materia di protezione dei
dati personali” e dell’art. 13 GDPR 679/16 – “Regolamento europeo sulla protezione dei dati personali”

Spoken English