PERAN DAN PERKEMBANGAN BIOTEKNOLOGI

DI ERA REVOLUSI INDUSTRI 4.0

KARTIKA SENJARINI

LABORATORY OF BIOTECHNOLOGY, DEPARTMENT OF BIOLOGY

FACULTY OF MATHEMATIC & NATURAL SCIENCES
UNIVERSITY OF JEMBER - INDONESIA
Univ. Muhammadiyah, Jan 4th 2019
Who am I? 

UB ITB - Universität Universität

Malang Bandung Rostock Kassel

Department of Biology
1 Faculty of Mathematics &
3
2
Natural Sciences
4 University of Jember
MOSQUITOE BASED VACCINE DEVELOPMENT
FOR MALARIA & DENGUE
OUTLINE
• BIOTEKNOLOGI
• BIOTEKNOLOGI MODERN BERBASIS
REKAYASA ORGANISME
• REVOLUSI INDUSTRI 4.0
DEFINITION of Biotechnology
• The broad area of biology involving living systems and
organisms to develop or make products, or "any
technological application that uses biological systems,
living organisms, or derivatives thereof, to make or modify
products or processes for specific use" (UN Convention
on Biological Diversity, Art. 2)
conventional Biotechnology
Biotechnology & Its application, Kevin Keener, 2012 - NC State Univ- US
ENGINEERING (REKAYASA)

modern Biotechnology
Biotechnology & Its application, Kevin Keener, 2012 - NC State Univ- US
REKAYASA TERHADAP KARAKTER
(FENOTIF)
 CELLS: FUNDAMENTAL UNIT OF LIFE

SINGLE CELL IS THE VEHICLE FOR ALL HEREDITARY INFORMATION THAT

DEFINE EACH SPECIES

Kartika Senjarini – Cell Biology I

All cells store their hereditary information in
the same linear chemical code: DNA

MEDIA FOR SAVING INFORMATION ON COMPUTER

Kartika Senjarini – Cell Biology I

 All cells store their hereditary information in
the same linear chemical code: DNA

DNA BUILDING BLOCK

Consequently, we can take a piece of DNA from a human cell and insert it into
a bacterium or vice versa and the information will be successfully read
Kartika Senjarini – Cell Biology I
Are these possible?...

Kartika Senjarini – Cell Biology I

Golden Rice
More interesting and advantageous for the consumer is the Golden Rice,
a product where a small biochemical pathway is constructed to produce
provitamin A. Three genes from different species were introduced into rice.

Narzisse/corn

Bakterium Erwinia

Rice

Large quantities of ß-carotin

(pro-vitamin A).

Abt. Genetik
Univ. Kassel
Even more impressive was the reconstruction of a plant biosynthetic
pathway in yeast to produce large amounts of artemisinic acid (malaria).

Abt. Genetik
Univ. Kassel
CRISPR/Cas System  „Gene Surgery“
In bacteria, a strange repeat sequence was detected in the genome.
There was no use or application.

After about 10 years of hard work, the CRISPR/Cas system is now

largely understood.

It is now used for genetic engineering in plants and animals

(genome editing)

It has the potential to deal with multiresistent bacteria

It has the potential for cancer therapy.

It would never have received funding for applied science.

CELL & TISSUE ENGINEERING
 Where are stem cells found?

embryonic stem cells tissue stem cells

blastocyst - a very early fetus, baby and throughout life
embryo
 Induced pluripotent stem cells (iPS cells)
genetic reprogramming
cell from the body (skin) pluripotent stem cell
(iPS)

differentiation
Stem cell therapy:
Not yet widely used (except for bone marrow transplants) but promising
for neurodegenerative diseases, diabetes, heart failure.
SIMPLE MODEL OF GERM LINE GENE THERAPY
In vivo - gene therapy - Ex vivo
BIO-FMIPA UJ: Salivary Mosquito based Transmission Blocking Vaccine
Tingkatkan imun nyamuk  parasit mati Hambat gamesitogenesis

Cegah perpindahan sporozoit

ke kelenjar saliva

Vaksin berbasis kelenjar saliva

Flying vaccinator
Identification of Immunogenic proteins from mosquitoes
salivary gland that mediate transmission of pathogen

Crude protein of SGE

Cut
off

Electroelution,
isolation &
purification
METHODS
Proteomic Analysis by using LC MS/MS

Desalting Process
(absorbed peptides)
Cut
off

1. Anhydration process : the gel dehydrated by using100 μl 100 % acetonitrile (ACN), dry up using the
vacuum centrifuge.
2. Reduction process : Protein were reduced by using 100 μL of 10mM DTT–100 mM am. bicarbonate.
3. Alkylation process by using 100 μL of 55 mM iodoacetamide–100 mM ammonium bicarbonate
4. Trypsin digestion : by using 10 mL solution of 50 mM ammonium bicarbonate in 5 mM CaCl2 / trypsin
5. Desalting process were washed with 100 μL of 0.1% TFA–2% ACN (solution A) and extracted with 100 μL
solution B (70% ACN, 0.1% TFA). Desalted peptide solution to be dried & keep in -20 before analized.
The digested and desalted peptides were identified by LC–MS/MS.

The digest & desalted peptides were identified by using LC MS/MS analysis Q-TOF
hybrid mass spectrometer (QSTAR Elite, AB SCIEX, Foster City, CA, USA)

The accumulated MS/MS spectra were processed by

using ProteinPilot software 2.0 (AB Sciex) against the
Aedes aegypti UniProt database (version 2013_10)
33
Big Data Analysis
35
„PEMBANTU“ BIOLOG
„PEMBANTU“

http://www.hackcollege.com

Biologi Terapan
ACKNOWLEDGEMENTS

• RISTEK DIKTI
• INTERNATIONAL VACCINE INSTITUTE, Seoul – South
Korea
• DAAD
• IGN
• UNEJ & FUAS
• IsDB