National Conference on

‘Frontiers in MS Technology
and Emerging Applications’
ITC Grand Chola, Chennai,
November 30, 2015.

Let noble thoughts come to us from every side.

- Rig Veda
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 Message from Director, IICMS

Dear Delegates,
On behalf of Indian Institute of Chromatography & Mass
Spectrometry (IICMS), the training arm of Spinco Biotech, it is my
great pleasure to welcome you all for this exciting National
Conference on 'Frontiers in MS Technology and Emerging
Applications'. Chennai, the southern gateway of India, well known
for its cultural activities setting the tone for such even!
Though MS is over 100 years old, it continues to pervade newer
areas of Science. IICMS offers a seamless connectivity between
you and the experts, elevating your knowledge on mass
spectrometry with its conglomeration of experts today both from
industry & academia providing a knowledge sharing platform
towards the application of this universal technique in relevant and
untapped areas.
As you wade through the program, you will realize that the expert
lectures cover the most recent application of mass spectrometry
including regulatory perspectives. Complementing this are over
40 posters on display covering recent applications. I encourage
you to interact with scientists as the mutual discussions can help
you on your scientic quest and also have a glimpse of the most
advanced liquid chromatography instruments displayed.
In this context, I express my gratitude to Prof.P.Balaram,
Dr.S.P.Vasireddi and other speakers who have been kind enough to
participate in this conference given their extremely busy schedule.
I am extremely thankful to Shuzo Maruyama for taking time out
exclusively for this event and the entire Shimadzu Asia Pacic,
Singapore and Shimadzu Analytical India Team, Mumbai for their
support.
Hosting a conference of this magnitude requires a strong team,
meticulous planning, execution and above all a strong leader! It is
none other than S. Thyagarajan, Chairman, Spinco Biotech who
has guided us all along to make this conference a great success.
The Team Spinco, pioneers in converting board room
'Discussions' to 'Actions', deserve unparallel appreciation for
their excellent support in bringing this elite group of experts and
users under one roof in Chennai!
Welcome once again to this exciting conference. Enjoy your
science!
Best regards,

Chennai
25-11-2015 Venkat Manohar
Director

3
Program
08:30 – 09:15 Registra on

Time Inaugural Session S p e a ke rs

09:15 – 09:30 Ligh ng the Lamp & Invoca on

09:30 – 09:40 Welcome & Opening Remarks Dr. Venkat Manohar, Director,
Indian Ins tute of Chromatography
and Mass Spectrometry, Chennai

09.40 – 10:10 Presiden al Address: Prof. P. Balaram,

‘Mass Spectrometric Sequencing Molecular Biophysics Unit,
of Conotoxins’ Indian Ins tute of Science, Bangalore

10:10 – 10:30 Address by Guest of Honour: Shuzo Maruyama, General Manager -

‘Shimadzu – Fast Growing Analy cal & Measuring Instruments
Leadership in Mass Spectrometry‘ Division, Shimadzu Corpora on, Japan

10.30 – 11:00 Keynote Address Dr. S.P. Vasireddi, Execu ve Chairman,

Vimta Labs Limited, Hyderabad

11.00 – 11:30 Networking Coffee Break

Session 2

11:30 – 11:50 Mass Spectrometry Applica ons Prof. Ram Rajasekharan, Director,
for Food Safety Central Food Technological Research
Ins tute, Mysore

11.50 – 12:10 Interface of Export Compliance Dr. Kaushik Banerjee,

and Mass Spectrometry: Principal Scien st – Agricultural Chemistry,
A Cri cal Roadmap Na onal Referral Laboratory,
ICAR – Na onal Research Centre for Grapes,
Pune

12:10 - 12:30 Diverse and Dynamic Challenges Dr. Nilanjana Biswas,

in Regulated Bioanalysis Principal Scien st – Clinical Res.,
Emcure Pharmaceu cals Limited, Pune

12:30 – 13:00 Feedback & Discussion

13:00 – 14:00 Networking Lunch

4
Session 3

14:00 – 14:20 LCMS-8060 Changes Everything Rashi Kochhar Rebello,

– Why & How? Senior Product Specialist – LC, LC/MS,
Shimadzu Analy cal (India) Pvt Ltd.,
Mumbai

14:20 – 14:40 Genotoxic Impuri es and Dr. Pritesh R. Upadhyay,

their es ma on by LC/MS Senior Vice President - Analy cal Research,
Lupin Research Park, Pune

14:40 – 15:00 PK Assessment Studies with Dr. Ravi K. Krovidi,

advanced LC-MS Technologies: Asst. General Manager (Biosimilars),
Comparison of a Candidate Lambda Therapeu c Research Ltd.,
Biosimilar to an Innovator Ahmedabad
Monoclonal An body

15:00 – 15:20 Biomarker and role of Dr. Ashutosh Pudage, President,

MS going ahead Accutest Research Laboratories
India Pvt Ltd, Mumbai

15:20 – 15:50 Feedback & Discussion

15.50 – 16:10 Networking Coffee Break

Session 4

16:10 – 16:30 Role of Mass Spectrometers Aslam Khan, Asst. Vice President &
in Regulatory Bioanalysis Head-Bioanaly cal, Reliance Life Sciences,
Mumbai

16:30 – 16:50 Mass-Omics: Fundamental Dr. Krishnan Venkateswaran,

Physics to Systems Biology Chief Scien st and Head R&D,
ITC Life Sciences and Technology Centre,
Bangalore

16:50 – 17:20 Feedback & Discussion

17:20 – 17:30 Closing Remarks C. Ravindranath, Managing Director,

Spinco Biotech Pvt Ltd., Hyderabad

5

Prof. P. Balaram
Molecular Biophysics Unit
Indian Institute of Science,
Bangalore
Presiden al Address
‘Mass Spectrometric Sequencing
of Conotoxins’
Prof. P.Balaram is a dis nguished scien st having done
extensive research on bioorganic chemistry and molecular
biophysics. He has carried out detailed studies on synthesis,
conforma onal analysis and biological ac vity of channel
pep des and has provided the basis for postula ng the helix
dipole model for these channels. Prof.Balaram has made
valuable contribu ons in the general area of design and
synthesis of model pep des, par cularly disulfides. He also
carried out conforma onal analysis using mass
spectrometry for proteins and pep des & computa onal
analysis on protein structures. He is the first researcher who
applied MALDI-TOF MS as well ESI-MS technology for
proteins & pep de analysis, in India. He was Director of
Indian Ins tute of Science (IISc), Bangalore and is currently
Professor-Emeritus at the Molecular Biophysics Unit at IISc.
 Prof. Balaram received his Bachelor's degree in
Chemistry from University of Pune & Master's degree
from the Indian Ins tute of Technology, Kanpur
 Ph.D degree from Carnegie-Mellon University, USA
 Postdoctoral s nt at Harvard University with Nobel
Laureate Robert Burns Woodward
Prof.P.Balaram is a Fellow of Indian Academy of Sciences,
Indian Na onal Science Academy. He has published more
than 400 papers including 4 book chapters and has held
editorial board memberships in many interna onal journals.
He has won many awards and honours such as INSA Medal
for Young Scien sts, SS Bhatnagar award, TWAS Award in
Chemistry, G.D. Birla Award for Scien fic Research and
Padma Shri by the Government of India.
6
 Address by Guest of Honour

‘Shimadzu – Fast Growing

Leadership in Mass Spectrometry’

Shuzo Maruyama was raised in Osaka, Japan. He

received his BS in Bio Physical Engineering from
Osaka University in 1982. Soon after graduation,
Shuzo Maruyama Shuzo started working for Shimadzu Corporation in
Managing Executive Kyoto Japan, as a R&D Engineer for HPLC products.
Officer, General Manager-
Analytical & Measuring He engaged in the development of LC-6A to LC-10A
Instruments Division series HPLC which were the driving products for
Shimadzu Corporation Shimadzu in analytical instruments business. After
Japan 15 years’ experience of R&D engineer, Shuzo was
promoted to R&D manager and then worked as
HPLC Business Unit Manager to expand Shimadzu
HPLC business worldwide (2004-2011).
Shuzo was assigned as the President of Shimadzu
Scientific Instruments in USA in 2011 and engaged
in the business development in US for 4 years with
great success. Shuzo worked as well as the
corporate officer of Shimadzu corporation
responsible for Analytical & Measurement Business
in North America. In June 2015, Shuzo was assigned
as Managing Exective Officer and the General
Manager of Analytical & Measurement Division of
Shimadzu Corporation.

7
 Keynote Address

Dr.Vasireddi founded Vimta Labs in 1984, which is

leading contract research and tes ng organiza on. His
Professional associa ons include: Member of Central
Advisory Commi ee of the Food Safety and Standards
Dr.S.P.Vasireddi Authority of India, (FSSAI), Government of India, New
Execu ve Chairman, Delhi., Member of the Consulta ve Group on Exports of
Vimta Labs Limited Pharmaceu cal Products under the chairmanship of
Hyderabad Commerce & Industry Minister, Government of India,
Ministry of Commerce & Industry Department of
Commerce, Member of the Technical Commi ee of the
Na onal Accredita on Board for Tes ng and Calibra on
(NABL), Member of Research Advisory Commi ee of
Medici Ins tute of Medical Sciences, Hyderabad.
His Past associa ons included: Founder Chairman of the
Associa on of Contract Research Organiza ons, India,
Regional Representa ve (Asia-Pacific) of the World
Associa on of Industrial and Technological Research
Organiza ons (WAITRO), Area-Coordinator of
Na onal Conference of Standards Laboratories (NCSL),
USA.
He established a training facility at Vimta in Public
Private Partnership with UNIDO (United Na ons
Industrial Development Organiza on) to provide
trainings to developing na ons, in the field of Food &
Pharma analy cal tes ng and quality systems.

8
 Prof. Ram Rajasekharan
Director
Central Food Technological
Research Ins tute, Mysore

Prof. Ram Rajasekharan is

a Ph.D in Biochemistry
from Indian Institute of
Science, Bangalore. His
research interests include
fats and oils as
nutraceuticals and dietary
supplements,
biochemistry and
molecular biology of plant
oils. Prof. Ram is a
J.C.Bose Fellow (2013) and
is a Fellow of Indian
National Science Academy
and Fellow of Indian
Academy of Sciences. He
has over fifty research
publications and several
patents to his credits.

9

Dr Kaushik Banerjee is a
Na onal Fellow and
Principal Scien st from the
ICAR-Na onal Research
Centre for Grapes,Pune. He
is a recipient of Fellow of
the Indian Na onal
Academy of Agricultural
Sciences (FNAAS) and
Fellow of the Royal Society
of Chemistry (FRSC),
London. He is the President
of the India Sec on of
AOAC Interna onal and is
also a member of the
Scien fic Panel of the FSSAI
on methods of sampling
and analysis. He does
extensive work on residue
analy cal methods using
GCMS and LCMS.
Dr. Kaushik Banerjee
Na onal Fellow and Principal Scien st
ICAR-Na onal Research Centre for Grapes
Pune
Interface of Export Compliance and
Mass Spectrometry: A Critical Roadmap
Mass spectrometry (MS) plays an important role in
trade related analytical compliance. MS, in
combination with liquid (LC-MS) and gas
chromatography (GC-MS) offers confirmatory
identification of contaminant residues in food
matrices along with their accurate quantification at
trace levels. Selectivity offered by unique mass
(m/z) and selective reaction monitoring (SRM)
transitions minimizes the chances of false
detections (false positives/negatives) and also
provides superior accuracy in quantitative residue
analysis. Residue analysis involving relatively non-
specific detectors on GC (e.g. electron capture
detector) and LC (e.g. UV detector) is no longer
accepted for trade related MRL (maximum residue
limit) compliance decisions. Tandem mass
spectrometry or MS/MS is considered as the gold
standard in residue analysis due to its high degree
of selectivity and sensitivity. In all cases analytical
quality control guidelines [e.g. method validation
guidelines of DG-Sanco (European Commission)]
needs to be strictly implemented to improve the
precision and accuracy of mass spectrometric
analysis and ensure analysis across the labs with
comparable efficiency. In addition to the MS/MS
based large scale targeted analysis, non-target
screening of residues based on full scan and
accurate mass based high resolution LC-MS are also
gaining significant applications in examining export
consignments.
10
 Dr. Nilanjana Biswas
Principal Scien st (General Manager)
/Head Bioanaly cal
Emcure Pharmaceu cal Ltd
Pune

Diverse and Dynamic Challenges in

Regulated Bioanalysis
Bioanalysis is an integral part of drug discovery and
development, spanning from early discovery to late
development stages. It is a tool to measure the
concentrations of the analytes (drug/
Dr.Nilanjana Biswas
metabolites) in different biological matrices
earned her Ph.D from obtained from in vitro screens or in vivo studies. It
University of Georgia,USA provides the foundation based on which the
and was a Post-Doctoral pharmacokinetics/toxicokinetics/ bioequivalence
Fellow in University of of the drug are determined.
Utah, USA. She has over
Since the EEC 65/65 directive in response to the
15 years experience in tragic incidence of phocomelia with thalidomide in
mass spectrometry, the mid 1900s, multiple regulations have been
chromatography and introduced in the pharmaceutical industry.
spectroscopy with special Bioanalysis being key in determining drug
emphasis in bioanalysis, exposures, that directly dictate safety and efficacy
metabolite iden fica on of the drug, has also evolved in the regulatory
and formula on analysis. space. However, despite the presence of
Besides, she is also multiple non-binding regulatory guidance's, the
working on areas such as industry is still faced with compliance
challenges, which are evident in the number of
ADME studies, cell
deficiencies received.
culture, IP rights, lipid
dynamics and protein Challenges in regulated bioanalysis are diverse and
structrues. dynamic. The challenges have constantly evolved
with the evolution of the industry pushing the
horizon to newer heights. For example, in the last
50-60 years, technological advancements have
made it possible to achieve 109 –fold higher
sensitivity. However, with these new possibilities,
new product profiles have come into the pipeline
for which just achieving pg/ml or fg/ml levels are
not sufficient.

11
 Rashi Kochhar Rebello
Senior Product Specialist – LC, LC/MS
Shimadzu Analy cal (India) Pvt Ltd.,
Mumbai

LCMS-8060: Changes Everything- Why

and How?
‘Changes everything’ is possibly the most apt
description for LCMS-8060. It has indeed changed
Rashi holds a M.Sc in the landscape of LC/MS/MS irrevocably. With
Bio-analytical and increasing demand from industry to measure at
Masters Degree in never seen trace levels, it was imperative for
Marketing Shimadzu to build a machine which is as fast and as
Management. She has sensitive as it gets. Enhanced ionization levels and
been with Shimadzu tremendous increase in ion transmission became
Analytical India Pvt Ltd the key area of work for engineers. This gave birth to
for over 7 years with newly designed UF-Qarray to achieve 90 times the
responsibilities primarily sensitivity of LCMS-8030!
focussed on LC and
LCMS. She is involved in But proof the pudding is in eating it, and proof of a
Application support, mass spec is testing the toughest molecule in
Product Management toughest matrix. For starters, we challenged the
and Customer Training machine with a steroid hormone as tough as
and Marketing Aldosterone. The results were astounding- 0.2
initiatives. pg/mL with a S/N of 6.8! Similar test are done with
array of small molecules like glucocorticosoids, lipid
etc, as well as with large molecules like MAb,
Insulin, glucagon etc, each giving astounding
results. All these results can be achieved within
regulated limits building desired confidence over
analytical experiments.

12

Dr.Ravi Krovidi focusses on
development of analytical
mass spectrometry-based
proteomics methodologies
for various clinical studies.
His group has developed
diverse LCMS based
methods, including
immunoaffinity strategies,
targetted proteomics,
surrogate peptide research
etc. He holds a Ph.D from
Max–Plank Institute,
Germany and has served
the U.S. Government,
Department of Energy
(DOE) Pacific Northwest
National Labs. His group at
Lambda is offering
advanced in-depth protein
characterization services to
various global biopharma
companies.
Dr. Ravi K. Krovidi
Asst. General Manager Biosimilars
Lambda Therapeu cs Research Ltd
Ahmedabad
PK Assessment Studies with Advanced
Liquid Chromatography and Mass
Spectrometry Technologies: Comparison
of a Candidate Biosimilar to an
Innovator Monoclonal Antibody
Biosimilars are biologics containing the same active
ingredient as the originator, they have the exact
amino acid sequence composition and highly
similar glycosylation patterns overlapping with the
originator reference product. Biosimilars should
demonstrate similarity to the innovator molecule at
levels including quality attributes, biological
activity, safety and efficacy based on extensive
comparability studies. Pharmacokinetic data
should demonstrate comparability regarding dose
exposure, safety, pharamcodynamics and
non-clinical and clinical response. LC-MS based
methods are currently evaluated for PK assessment
of various mAb's.
13

Dr.P.R.Upadhyay has a Ph.D
in Analytical Chemistry of
Cephalosporins Antibiotics
and is serving as Senior Vice
President (Analytical
Research) at Lupin Research
Park, Pune. His
responsibilities include
taking care of entire
analytical activity for Active
Pharmaceutical Ingredients
and drug products. He has
more than twenty nine years
of widespread leadership
experience having worked
on diverse analytical
projects in industrial
environment.
Dr. P. R. Upadhyay
Senior Vice-President Analy cal Research
Lupin Research Park
Pune
Quetiapine MRM validation method for
2 Chloroethoxy ethanol
A new method was developed to determine 2
Chloroethoxy ethanol in Quetiapine using liquid
chromatography tandem mass spectrometry
(LC–MS/MS) with electrospray ionization (ESI). 2
Chloroethoxy ethanol is a potential genotoxic
impurity, resulting in the need for suitable
analytical methodology which can accurately
determine its level at several orders of magnitude
less than typical impurity quantitation limits. The
limit for 2 Chloroethoxy ethanol is 2 ppm in
Quetiapine based on daily dose. Chromatography
was performed on a Zorbax RX C-8,(4.6 x 250mm),
5mm analytical column with a mobile phase of 0.1%
trifluoroacetic acid in water and acetonitrile, at a
flow rate of 1.0 mL/min. The method was fully
validated in terms of selectivity, limits of detection
(LOD) and quantification (LOQ), linearity, carry-over
effect, reinjection reproducibility, precision and
accuracy. The LOD and LOQ values were below 0.32
ppm and 1 ppm with respect to test, respectively.
The proposed method was applied to analyze
Quetiapine API samples.
14

Aslam Khan has a Master's
Degree in Analytical
Chemistry from Jiwaji
University, Gwalior and has
more 17 years of
experience in various
pharmaceutical companies.
He has done extensive
work on method
development for
formulations, impurity
profiling, bioassays, BA/BE
studies, pharmacokinetics,
bioanalytical methods etc.
He has set up a world class
bio-analytical facility at
Reliance Clinical Research
Services and has provided
guidance to develop and
validate more than 200
analytical methods for
BA/BE studies.
Aslam Khan
Asst. Vice President & Head - Bioanaly cal
Reliance Clinical Research Services
Mumbai
Role of LC/MS in Regulated Bioanalysis
Liquid chromatography coupled with mass
spectrometer is an analytical technique that plays a
very important role in industrial analysis.
Bioanalysis is regulated analysis as it is indicative of
the safety aspects of the drugs and their related
substance of interest. There are various bio
analytical applications that require to employ
advances techniques like LC/MS and LC/MS-MS to
get accurate, precise and specific results to be able
to draw conclusive inference of a trial or an
experiment. In this presentation, presenter will try
to cover some regulated and complex analytical
applications that are simplified using LC/MS as the
analytical tool.
15
 Dr. Krishnan Venkateswaran
Chief Scien st & Head R&D
ITC Life Sciences and Technology Centre
Bangalore

Mass-omics: Fundamental Physics to

Systems Biology
Systems biology is characterized as the quantitative
analysis of dynamic interactions between the
components of complex biological systems with the
aim of understanding the behavior of the system as a
Dr. Krishnan whole, through predictive computational modeling.
Venkateswaran is a Ph.D In place of reductionist approach of molecular
from USA and has done biology, systems biology aims at systems-level
extensive research in understanding of correlations among molecular
chemical physics at components. Currently, ‘systems biology’ is being
explored in several fields of biosciences and all
University of Bri sh
‘Omics’ platforms fall under system biology
Columbia, Vancouver, approach, e.g., metabolomics, proteomics,
Canada. He has over 25 genomics, bioinformatics, to name a few. Mass
patents and 50 research spectrometry, being one of the most versatile and
publica ons and has powerful technique to identify and characterize not
more than 20 years of only simple but also highly complex molecular
experience in the Personal structures such as large biomolecules of proteins &
Care domain. He has peptides, has already been realized as an
made invited scien fic indispensable tool to understand systems biology.
contribu ons at the The high-resolution/high-accuracy MS techniques
developed in recent past, such as, TOF, FT-ICR and
pres gious Royal Society
Orbitrap, with capability to acquire multiple mass-
of Chemistry UK, Faraday spectra/second, have been interfaced with powerful
Discussion mee ngs. chromatographic separations (e.g. Nano-LC, GC, CE
etc.) to yield structural information of the individual
components. Here we will discuss two of the major
areas of ‘System Biology’ viz., metabolomics and
proteomics, where mass-spectrometry, a branch of
molecular physics plays a vital role to help elucidate
the comprehensive analysis and overall outcomes.
Metabolomics and proteomics is the study of
organismal, tissue, or cell level measurement of
proteins, and metabolites, respectively. The
presentation illustrates application of modern MS
techniques in Systems Biology: (a) In metabolomics,
(b) In proteomics.

16
 Poster Presenta ons
A High Sensi vity LC/MS/MS Method with QuEChERS Sample
Pre-treatment for Analysis of Aflatoxins in Peanut Bu er Samples
Yin Ling Chew¹; Jie Xing¹; Zhi Wei Ting¹; Jun Xiang Lee²; Zhaoqi Zhan¹
¹Shimadzu (Asia Pacific) Pte Ltd, Singapore, ²School of Physical & Mathema cal
Sciences, Nanyang Technological University, Singapore

A Non-invasive approach towards the quan fica on of hyperglycemia

using LCMSMS technique as a diagnos c device
Priyadarshini SK¹; Sathasivasubramanian.S¹; Kannadhasan Umaiyorubagan²;
Vennapusa Chandra Sekhara Reddy²; Saravanan Subramanian²; Mohan Kasi² and
Venkat Manohar²
¹Sri Ramachandra University, Porur, Chennai, INDIA. ²Indian Ins tute of Chromatography &
Mass Spectrometry, Chennai

A Validated Stability Indica ng Analy cal Method For

Erlo nib Hydrochloride From Its Forced Degrada on Study
B. Babu¹, S. N. Meyyanathan¹, B.Gowramma², ¹Department of Pharmaceu cal
Analysis, ²Department of Pharmaceu cal Chemistry,
J.S.S. College of Pharmacy, (Cons tuent college, J.S.S. University, Mysore) Udhagamandalam,
Nilgiris, Tamilnadu.

Analysis of Chloramphenicol in Shrimp by LC/MS/MS

using Modified QuEChERS Sample Pre-treatment
Pei ng Zeng¹, Choo You Zhen Leonard ², Jie Xing¹, & Zhaoqi Zhan¹
¹ Customer Support Centre, Shimadzu (Asia Pacific) Pte Ltd, Singapore ²
Singapore Polytechnic, Singapore.

Applica ons of Shimadzu LCMS-2010 EV Mass Spectrometer in

Tuberculosis research
Hemanth Kumar¹, A.K., Sivaram¹, V., Saravanan¹, N., Kiran Kumar¹, A., Sudha¹, V.,
Sagarika Devi²., Anusha³, B., Geetha Ramachandran¹
¹ Na onal ins tute for Research in Tuberculosis (ICMR), Chennai ² Murugappa Che ar
Research Centre (MCRC), Chennai ³ Sathyabama University, Chennai

Chloro Genotoxic Impurity is quan fiable down to

ppb level through SSS technique using LCMS-8040 Triple Quad
Saravanan Subramaniyan; Janani Thyagarajan; Vinod Pillai; Rampriya
Udhayakumar; Mohan Kasi & Venkat Manohar
Indian Ins tute of Chromatography & Mass Spectrometry, Chennai

Compara ve Analysis of Phyto-chemical cons tuents in

Fig Wine and Grape Wine using GC-MS
Udaykumar Nidoni, Nagaraj Naik, M. Bheemanna, Vishal B and Dinesha B. L.
Department of Processing and Food Engineering, College of Agrilcultural Engineering
University of Agricultural Sciences, Raichur, Karnataka

17
 Poster Presenta ons

Determina on of chemical contaminants in marine fish by

GCMS/MS using QuEChERS as an extrac on method
Ankush Bhone, Durvesh Sawant, Dheeraj Handique, Prashant Hase,
Sanket Chiplunkar, Ajit Datar, Jitendra Kelkar & Pratap Rasam
Shimadzu Analy cal (India) Pvt Ltd, Mumbai

Determina on of Leachables in Orally Inhaled and Nasal

Drug Products (OINDP) by GCMS/MS
Prashant Hase, Ankush Bhone, Durvesh Sawant, Dheeraj Handique,
Sanket Chiplunkar, Ajit Datar, Jitendra Kelkar & Pratap Rasam
Shimadzu Analy cal (India) Pvt Ltd, Mumbai

Determina on of Vola le Organic Compounds (VOCs) present in the

interiors of car by using GCMS/MS with sta c and dynamic headspace
Sanket Chiplunkar, Ankush Bhone, Durvesh Sawant, Dheeraj Handique,
Prashant Hase, Ajit Datar, Jitendra Kelkar and Pratap Rasam
Shimadzu Analy cal (India) Pvt Ltd, Mumbai

Development and Valida on of LC-MS/MS Method For The Es ma on

of Zaltoprofen Enan omers In Pharmaceu cal Formula on
B. GOWRAMMA¹, B. BABU², S. N. MEYYANATHAN² ¹Department of
Pharmaceu cal Chemistry ²Department of Pharmaceu cal Analysis,
JSS College of Pharmacy, (Cons tuent College of JSS University, Mysore) Rocklands,
Udhagamandalam, Tamilnadu

Development of LC/MS/MS Method for Quan ta ve Analysis of

25-Hydroxyvitamin D2 and D3 in Human Plasma
Zhi Wei Edwin Ting¹, Jun Xiang Lee², Jie Xing¹, & Zhaoqi Zhan¹
¹ Customer Support Centre, Shimadzu (Asia Pacific) Pte Ltd, Singapore ² School of Physical &
Mathema cal Sciences, Nanyang Technological University, Singapore.

Development of LC-TQ-MS based quan ta ve condi on of

CDR pep des in an body drugs by nSMOL protocol
Noriko Iwamoto¹, Takashi Shimada¹, Akinobu Hamada²,
¹Life Sci. Res. Ctr., Shimadzu Corp., Japan, ²Clin. Pharm. Transl. Res., Na onal Cancer Center, Japan

Effect of quorum sensing inhibitor curcumin on proteome of

Pseudomonas aeruginosa PAO1
Sivasamy Sethupathy, Krishnan Ganesh Prasath, Samiraj Yesu Balan &
Shunmugiah Karutha Pandian
Department of Biotechnology, Alagappa University, Science Campus, Karaikudi, Tamil Nadu

18
 Poster Presenta ons

Evalua on of garlic organosulfur vola les, diallyl disulphide and

diallyl sulphide as a new an -glyca ng compound by matrix-assisted laser
desorp on ionisa on mass spectrometry – an insulin based glyca on assay
Murali Mohan Bhandi¹, Roshan M. Borkar¹, Sanjay K. Benerjee², R. Srinivas¹
¹Na onal Centre for Mass Spectrometry,
CSIR-Indian Ins tute of Chemical Technology, Hyderabad
²Medicinal Chemistry and Pharmacology Division, CSIR-Indian Ins tute of Chemical,
Technology, Hyderabad

Effect of quorum sensing inhibitor curcumin on proteome of

Pseudomonas aeruginosa PA01
Sivasamy Sethupathy, Krishnan Ganesh Prasath, Samiraj Yesu
Balan and Shunmugiah Karutha Pandian
Department of Biotechnology, Alagappa University, Science Campus,
Karaikudi, Tamil Nadu

High Sensi vity Bioanaly cal Method on LCMS-8060:

Entecavir in Human Plasma
Zhi Wei Edwin Ting¹, Yu Jun Stephanie Tan², Jie Xing¹, & Zhaoqi Zhan¹
¹ Customer Support Centre, Shimadzu (Asia Pacific) Pte Ltd, Singapore
² School of Physical & Mathema cal Sciences, Nanyang Technological University, Singapore

High sensi vity Bioanaly cal Method: Atorvasta n Calcium in Human Plasma
Zhi Wei Edwin Ting¹, Yu Jun Stephanie Tan², Jie Xing¹, & Zhaoqi Zhan¹
¹ Customer Support Centre, Shimadzu (Asia Pacific) Pte Ltd, Singapore
² School of Physical & Mathema cal Sciences, Nanyang Technological University, Singapore

Highly sensi ve quan ta ve analysis of Amoxicillin and Clavulanic acid

from plasma using LC/MS/MS
Deep Bhandarkar, Rashi Kochhar, Shailendra Rane, Shru Raju, Shailesh Damale,
Ajit Datar, Pratap Rasam and Jitendra Kelkar
Shimadzu Analy cal (India) Pvt Ltd, Mumbai

Highly sensi ve quan ta ve analysis of Felodipine and

Hydrochlorothiazide from plasma using LC/MS/MS
Shailendra Rane, Rashi Kochhar, Deep Bhandarkar,
Shru Raju, Shailesh Damale, Ajit Datar, Pratap Rasam, Jitendra Kelkar
Shimadzu Analy cal (India) Pvt Ltd, Mumbai

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 Poster Presenta ons

Iden fica on of Trace Impurity of Capecitabine by LC/MS/MS;

Its Cri cal Isola on and Characteriza on
Rathnakar Reddy Kura, Yogeshwar Reddy Mamilla, Madhavi Egumamidi,
Nagender Reddy Purumandla, Ramakrishna Reddy Kuppireddy, Sudhakar Reddy
Pondugula, Sri Shivakumar Gubbala and Shanker Kanne
Hetero Research Founda on, Balanagar, Hyderabad

Isola on, Purifica on and Characteriza on of Clinically important

an oxidant lignans - Sesamin and Sesamolin, from Sesame Oil
Aejaz Ahmad Dar, Ni sh Kumar Verma and Neelakantan Arumugam
Department of Biotechnology, School of Life Sciences, Pondicherry University, Pondicherry

LCMS/MS – A tool for the detec on of phenolics in Psychotria flavida

Sana Sheik, Bhagya N., Chandrashekar K. R.
Department of Applied Botany, Mangalore University, Mangalagangotri,
Mangalore, Karnataka

Low level quan ta on of steroids in milk using LC/MS/MS

Durvesh Sawant, Shailesh Damale, Deep Bhandarkar, Shailendra Rane,
Rashi Kochhar, Shru Raju, Ajit Datar, Pratap Rasam and Jitendra Kelkar
Shimadzu Analy cal (India) Pvt Ltd, Mumbai

Matrix-Assisted Laser Desorp on/Ioniza on Mass Spectrometry Analysis of

Free Amino Acids and Methylated Amino Acids: Metabolomics Perspec ve
Sudarshana Reddy. B, Sony. T, Prasada Raju. N, Prabhakar. S
Na onal Centre for Mass Spectrometry, CSIR-Indian Ins tute of Chemical Technology,
Hyderabad

Perchlorate determina on in environmental samples

using Shimadzu LCMS-2020
M.R. Sijimol, D. Dineep and Mahesh Mohan
School of Environmental Sciences, Mahatma Gandhi University, Ko ayam, Kerala

Proteomic analysis on the an biofilm ac vity of

limonene against Streptococcus pyogenes
Ashwinkumar Subramenium, Amos Rabinson, and Shunmugiah Karutha Pandian
Department of Biotechnology, Alagappa University, Science Campus, Karaikudi, Tamil Nadu

GC-MS/MS and LC-MS/MS Analysis of Bioac ve Compounds from

Supercri cal Fluid and Soxhlet Extracted Moringa (Moringa
oleifera Lam.) Seed Kernel Oil
Dinesha, B. L, Udaykumar Nidoni, Nagaraj Naik and Ramachandra C. T
Department of Processing and Food Engineering, College of Agricultural Engineering,
University of Agricultural Sciences, Raichur, Karnataka

20
 Poster Presenta ons
Quan fica on and Valida on of Querce n in Monochoria vaginalis
(Burm. f.) C.Presl and Monochoria hastata (L) Solms leaves by LC-MS
N. Kasthuri bai, K. Ilango and R. Mohankumar
Interdisciplinary Ins tute of Indian System of Medicine (IIISM), SRM University,
Ka ankulathur Kancheepuram (Dt), Tamil Nadu

Quan fica on of insulin in human serum samples by Matrix Assisted

Laser Desorp on Ioniza on-Time of Flight Mass Spectrometry
C. Shireesha, N. Prasad Raju, V. Naga Veni, U.V.R. Vijayasarathi
¹Na onal Centre for mass spectrometry, CSIR-Indian Ins tute of Chemical Technology,
Hyderabad

Quan ta ve analysis of illegal dyes in eggs using LC/MS/MS

Rashi Kochhar, Shailendra Rane, Shailesh Damale, Deep Bhandarkar,
Shru Raju, Ajit Datar, Pratap Rasam and Jitendra Kelkar
Shimadzu Analy cal (India) Pvt Ltd, Mumbai

Selec ve detec on of CDR pep des of monoclonal an bodies for

LCMS-based therapeu c drug monitoring by nano-surface and
molecular-orienta on limited (nSMOL) proteolysis
Noriko Iwamoto¹, Takashi Shimada¹, and Akinobu Hamada²
¹ Life Sci. Res. Ctr., Shimadzu Corp., Japan
² Clin. Pharm. Transl. Res., Na onal Cancer Center, Japan

Simultaneous LC-MS/MS analysis of natural pigments and

Monocolin from Monascus ruber
R. Paranthaman, R.Vidyalakshmi, K.Srinivasan and S. Kumaravel
Department of Food Safety and Quality Tes ng
Indian Ins tute of Crop Processing Technology (IICPT), Thanjavur, Tamil Nadu.

Speciality Bio Analy cal Solu ons using MS Technology

Perfomics Analy cal Labs LLP, Hyderabad

The “SSS” tool of LCMS-8040 unveils the structure of

Lactam impuri es in Olanzapine !!!
Janani Thyagarajan; Raman Palavannathan; Vijaya Phani Kumar Yemparala;
Saravanan Subramaniyan; Kandasamy Sakthivel; Nagasivarao Jonnaladda;
Mohan Kasi; Venkat Manohar;
Indian Ins tute of Chromatography & Mass Spectrometry LLP, Chennai

Monitoring of Emerging Contaminants

Group Head: Dr.R.Babu Rajendran, Professor&Head,
Department of Environmental Biotechnology, Bhara dasan University,
Tiruchirapalli.

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info@spincotech.com
www.spincotech.com

23
 Shimadzu Corporation

LCMS-8060

World’s highest sensitivity - Unmatched speed - Outstanding durability

 First Mass Spectrometry company in the world to deliver Triple Quadrupole MS with highest
sensitivity and ultra-fast technologies
 Ultra sensitive detection in bioanalysis
 Newly developed UF-Qarray ion guide – a groundbreaking technology
 Ultra fast scan speeds at 30,000 u/sec and a UF polarity switching of 5msec, make a real
difference to working better and faster
 Advanced Ion guide for increased ion production and detection.
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