Discussion

 Advantages of Nanodrop over cuvette based spectrophotometry is that it is capable of

measuring very concentrated samples without dilution – it is 50 times higher than
measurements by standard cuvette based spectrophotometry. Also it has a sample
retention technology that uses only surface tension to hold the sample thus there is no
need in cuvettes.
 Cheek cell DNA extraction is the extraction of the genomic DNA; therefore, it is easier to
isolate the whole genomic DNA because it is just needed a good lysis step to release
genomic DNA. Whereas, extracting plasmid DNA is a bit more complicatd because it
requires separating plasmid from the genomic DNA. In addition, in isolating genomic
DNA, chewing the cheeks was the primary method of cell lysis (physical) whereas in
plasmid extraction SDS-alkaline method was applied for the lysis.
 Culturing bacteria for 16 hours is optimal as it is the transition from logarithmic into
stationary growth phase. At this time, the ratio of plasmid DNA to RNA is higher than
during the logarithmic phase. If culturing last longer, DNA might be degraded due to
over aging of the culture in the later stationary phase.