Enzy me activity, V (mo l/min)

Temperature (oC) Enzyme activity, V (mol/min)

0.0000012
30 0.000000933
40 0.000000794
50 0.000000966 0.000001

60 0.000000921
70 0.00000104 0.0000008

0.0000006

0.0000004

0.0000002

0
25 30 35 40 45 50 55 60 65 70 75

Temperature (oC)
A b s o rb a n c e O p t i c a l D e n si t y (O D )(n m )

pH value Absorbance Optical Density (OD)

5 10.58
6 8.13
7 9.47
8 9.9 12

10

6
 A b s o rb a n c e O p
12

10

0
4.5 5 5.5 6 6.5 7 7.5 8 8.5

pH value

Enzyme activity, V (mol/min)

pH Enzyme activity, V (mol/min)
5 0.00000113
6 0.000000866 0.0000012
7 0.00000101
8 0.00000105
0.000001

0.0000008

0.0000006

0.0000004

0.0000002

0
4.5 5 5.5 6 6.5 7 7.5 8 8.5

pH Value
 0.0000002

0
4.5 5 5.5 6 6.5 7 7.5 8 8.5

pH Value

A b s o rb a n c e O p t i c a l D e n si t y (O D ) (n m )
Substrate Concentration Absorbance Optical Density (OD)
0.5 10.57
1.5 7.8
2 9.32 12
2.5 8.88
3 7.92 10

0
0 0.5 1 1.5 2 2.5 3 3.5

Substrate Concentration (%)

0.0000012

Substrate Concentration Enzyme activity, V (mol/min)

0.5 1.13 × 10-6 0.000001
1.5 8.33 × 10-7
zyme activity, V (mol/min)

2 9.94 × 10-7
0.0000008
2.5 9.49 × 10-7
3 8.44× 10-7
0.0000006

0.0000004
 Enzyme activity, V (mol/min)
0.0000008

0.0000006

0.0000004
Substrate Concentration Enzyme activity, V (mol/min)
0.5 0.00000113
1.5 0.000000833 0.0000002
2 0.000000994
2.5 0.000000949
0
3 0.000000844 0 0.5 1 1.5 2 2.5 3 3.5

Substrate Concentration (%)

1.40E+06

1/S 1/V
2 8.85 × 105 1.20E+06

0.67 1.20 × 106 f(x) = - 137657.07757815x + 1172372.52051096

0.5 1.01 × 106 R² = 0.5438582901
1.00E+06
0.4 1.05 × 106
0.33 1.18 × 106
8.00E+05
1/V

1/S 1/V 6.00E+05

2 8.85E+05
0.67 1.20E+06
0.5 1010000 4.00E+05
0.4 1050000
0.33 1180000 2.00E+05

0.00E+00
0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2

1/S
0.00E+00
0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2

1/S
3.5
2 2.2
2 2.2