See discussions, stats, and author profiles for this publication at: https://www.researchgate.

net/publication/293977363

Additional file 1

Data · February 2012

CITATIONS READS
0 95

4 authors, including:

Lily Tan Rajagopalan Srinivasan

Institute of Chemical and Engineering Sciences Indian Institute of Technology Madras
12 PUBLICATIONS   54 CITATIONS    288 PUBLICATIONS   3,350 CITATIONS   

SEE PROFILE SEE PROFILE

Some of the authors of this publication are also working on these related projects:

Modelling and Simulation of Heat Recovery Steam Generator View project

All content following this page was uploaded by Rajagopalan Srinivasan on 15 February 2016.

The user has requested enhancement of the downloaded file.

 Calculation of growth and non-growth associated maintenance requirements for Sc. stipitis

The growth and non-growth associated maintenance coefficients (GAM and NGAM) for Sc. stipitis were

estimated by plotting the glucose uptake rate against the growth rate. Data obtained from our experiments

and that reported in literature (van Urk et al, 1990) was used. Model simulations were carried out for

various values of GAM and the results were plotted with experimental data as shown in figure below.

GAM of 44.4 was found to match with experimental data. The NGAM was calculated from the y-

intercept. By maximizing ATP turnover under the glucose uptake constraint of 1 mmol/gDCW-hr, the

ATP yield is evaluated as YATP, max = 28.6 mol ATP/ mol glucose. Using this value and the y-intercept

(0.095 mmol glucose/gDCW-hr), we can calculate the NGAM requirement to be about 2.7 mmol

ATP/gDCW/hr

4.5

3.5
Glucose uptake rate mmol/gDCW/h

2.5

2
Experimental Data
1.5 GAM = 24.4
GAM = 34.4

1 GAM = 44.4
GAM = 54.4
GAM = 64.4
0.5
Linear (Experimental Data) y = 10.24x + 0.095

0
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5
Growth Rate (h-1)

References

van Urk H, Voll WSL, Scheffers WA, van Dijken JP (1990) Transient-state analysis of metabolic fluxes
in crabtree-positive and crabtree-negative yeasts. Appl Microbiol Biotechnol 56(1): 281-287.

View publication stats