Scientific Research II Lit Review Matrix

Article Title & Link Theoretical/Conceptual
(Add APA citations below Framework
matrix
Using www.citefast.com)
Maternal germ-line Previous studies have
transmission of mutant introduced mtDNA
mtDNAs from embryonic mutations into whole-
stem cell-derived chimeric animal systems but not in
mice. such a way that these
mutations are then
https://www.ncbi.nlm.nih.g inherited via maternal
ov/pmc/articles/PMC18941/ lineage in subsequent
# generations.
CRISPR, the disruptor A new gene-editing
technology, known as
https://www.nature.com/ne CRISPR, is making a big
ws/crispr-the-disruptor- impact in the science of
1.17673 biology with great
potential as well as
concerns.
Research Methodology Analysis & Results Conclusions Implications for
Question(s)/Hypo Future Research
thesis
Example
Can mtDNA 1. Embryonic stem CAPr mtDNA can be This new method This procedure
embryonic stem cell culture & transmitted through makes it possible can now be used
cells be introduced cybrid preparation. maternal germ line; to introduce to study several
into female mice 2. Production & however, the pups did mouse mtDNA new mtDNA
in such a way that Genotyping of not live beyond into female germ mutations.
it will then be Chimeric & neonatal period. line via somatic
inherited in Transgenic Mice cells, resulting in
subsequent 3. Pathological inheritance in
maternal germ analysis subsequent
lines? 4. Electroretino- generations.
graphy
If there is a way to The article is based upon Despite its value in There are One implication is
quickly alter DNA the impact of CRIPSR to biology, scientists have obstacles to to try and cure
at a reasonable further my knowledge ethical and safety CRISPR that diseases with
price, scientists on its potential and the concerns for future may be difficult caution because
can learn about the reasons for its immense experiments. On the to accomplish, mutations and
different genomes success. Therefore there other hand, it has the but it offers great cancer growth are
of humans along is not an exact potential of eliminating value to the a huge concern.
with any other procedure. diseases, creating community of Another would be
organism. This is hardier plants, and science. It is making crops
particularly useful wiping out pathogens. important that it healthier and
for the study of It is also expanding the isn’t used for the stronger but
pathology. possibility to edit the wrong reasons opponents of
genes of more and to be careful genetically
organisms whereas when altering the modified crops
scientists were more genes of certain may hinder this.
constricted to animals aspects such as Finally there is
such as fruit flies and food and animals. the implication of
mice. changing animals’
genes, possibly
wild ones too.

What is CRISPR gene CRISPR has recently What does The article helps explain CRISPR is a system in CRISPR is a Stem cells are
editing, and how does it become more popular in CRISPR/Cas9 do? what CRISPR is, how to bacteria that bacteria unique system to being modified in
work? (cover story) the scientific world and it What are we doing use it, and how scientists use to cut up DNA of learn about more research to

http://eds.a.ebscohost.com/e
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The CRISPR Pioneers.
http://eds.a.ebscohost.com/e
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Comparative analysis of the
response and gene
regulation in cold resistant
and susceptible tea plants.
http://eds.a.ebscohost.com/e
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id=17&sid=6661bdb6-9aec-
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Scientific Research II Lit Review Matrix
is important for newcomers
to know what it is and how
it works.
CRISPR has been used in
many different ways but
there a few incredible
scientists that have used it
in staggering ways that
could ultimately change
the way we live.
Cold environments are one
of the major obstacles of
tea plants but by
comparing tea plants with
high cold-tolerance and
low cold-tolerance, there
will be a better
understanding of how their
response mechanisms and
they can be bred better.
with are using it to further my invading bacterial genomes and has replenish
CRISPR/Cas9? understanding on the viruses that oppose a provides many damaged organs
topic. threat to them, but opportunities to which may help
today it is being used to better science in be life changing in
alter the DNA of any with a variety of the future. Plants
organism. It works by possibilities. are also another
linking an artificial potential
guide to a specific implication for
segment of DNA to cut future research
and then insert a new because there is
gene in because the cell an abundance of
will try to repair itself research that can
by using any available be done.
DNA it can find.
What are some This article provides A few mind-blowing CRISPR still has In the future,
important ways information on scientists ways CRISPR has been a long way to go, scientists can find
that CRISPR who have used CRISPR used includes treating but it continues ways to treat
technology has in many impressive cancer, researching to exceed chronic illnesses
been used to ways. treatment for HIV, and expectations by such as cancer,
improve our attempting to study the studies and HIV, Type 2
society? genetic diseases experiments that diabetes,
including sickle cell have been Alzheimer’s and
anemia, cystic fibrosis, performed. genetic diseases
and chronic conditions by using CRISPR.
such as Type 2 diabetes
and Alzheimer’s.
If we compare the 1. Clone cuttings of Increased expression of The main genes Similar
physiological and Camellia sinensis cv. CsCBF1 and CsDHNs responsible for experiments can
biochemical Shuchazao and Camellia coupling with the regulation in cold be conducted to
responses, and sinensis var. assamica accumulation of resistance have find important
associated gene cv. Yinghong9 were sucrose play key roles been discovered, information on
expression of the collected in conferring higher helping the cold-resistance
tea plants in 2. One-year-old cutting- cold resistance in SCZ understanding of genes and
response to o 7- propagated plants were cold resistant breeding in
day and 14-day transferred to a growth mechanisms and different types of
cold acclimation, chamber with breeding for tea plants.
followed by 7-day temperature cycles of plants.
de-acclimation in 25˚C at day time and
these two tea 20˚C at night time, 12 h
cultivars, we will photoperiod, and 70%
better understand relative humidity for one

An Overview of Cold
Resistance in Plants.
http://eds.a.ebscohost.com/e
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Scientific Research II Lit Review Matrix
Cold temperatures are an
environmental stress factor
that restricts geographical
location and growth of
various plants. It affects
crop quality and
productivity; however,
cold-resistant genetic
engineering development
has led to astonishing
progress.
the response month
mechanism of tea 3. The plants were put
plants under cold into different degrees of
stress for cold acclimation and de-
improving acclimation
breeding. 4. Ten well-grown one
year-old tea plants were
collected and used as
non-acclimation
5. The plants were
exposed to normal
temperature (25/20˚C,
day/night temperature)
for 7 days for de-
acclimation. At each
time point, the leaves
were collected,
immediately frozen in
liquid nitrogen and
stored at −80˚C until use
What are major The article aims to The research paper Unsaturated fatty In the future there
components that provide information on provided extremely acids, protective can be more
affect cold cold resistance in plants useful facts such as cell enzymes, research on plant
resistance in rather than perform an membranes are where functional genes, cold resistance
plants? experiment. most cold-induced and regulation because it is
injuries originate, the genes all relatively weaker.
manipulation of various contribute to cold
genes is a property of resistance factors
cold resistance in found in plants
plants, and the activity which can be
of antioxidant enzymes used in future
serve the role in research and
inhibiting membrane experiments.
proteins and membrane
lipid peroxidation
which can improve the
cold resistance of the
plant.
Citations
 Scientific Research II Lit Review Matrix
Example

Sligh, J. E., Levy, S. E., Waymire, K. G., Allard, P., Dillehay, D. L., Nusinowitz, S.,Wallace, D. C. (2000). Maternal germ-line transmission of mutant mtDNAs
from embryonic stem cell-derived chimeric mice. Proceedings of the National Academy of Sciences, 97(26), 14461-14466. doi:10.1073/pnas.250491597

Jinek, M., Liang, P., Vyas, V. K., Barrasa, M. I., Fink, G. R., Maddalo, D., Yin, H, Oye, K. A., Gantz, V. M. & Bier, Ishino, Y., Shinagawa, H., Makino,
K., Amemura, M. & Nakata, A., Mojica, F. J., Ferrer, C., Juez, G. & Rodríguez-Valera, F., Barrangou, R., Cong, L., Mali, P. (2015). CRISPR, the
disruptor. Nature. Retrieved from https://www.nature.com/news/crispr-the-disruptor-1.17673
Crossley, M. (2018). What is CRISPR gene editing, and how does it work? (Cover story). Chemistry in Australia, 18–19. Retrieved from
http://search.ebscohost.com/login.aspx?direct=true&db=a9h&AN=130038067&site=ehost-live
Park, A. (2016). The CRISPR Pioneers. TIME Magazine, 188(25–26), 116–122. Retrieved from
http://search.ebscohost.com/login.aspx?direct=true&db=a9h&AN=120052882&site=ehost-live
Ban, Q., Wang, X., Pan, C., Wang, Y., Kong, L., Jiang, H., … Jiang, C. (2017). Comparative analysis of the response and gene regulation in cold resistant and
susceptible tea plants. PLoS ONE, 12(12), 1–18. https://doi.org/10.1371/journal.pone.0188514
Chen, L. ‐J., Xiang, H. ‐Z., Miao, Y., Zhang, L., Guo, Z. ‐F., Zhao, X. ‐H., … Li, T. ‐L. (2014). An Overview of Cold Resistance in Plants. Journal of Agronomy
& Crop Science, 200(4), 237–245. https://doi.org/10.1111/jac.12082