METTLER TOLEDO Sodium Analyzer AP201

Sodium Content in Potato Chips

The sodium content of potato chips after preparation of a sample dilution solution was determined by multiple
standard addition technique using the sampling type "aliquot excluding ionic strength adjuster (ISA)".

Sample Potato chips, 15 g

Preparation Preparation - CAUTION
procedures  Use safety goggles, a lab coat and wear gloves. If possible, work under a fume
hood.
 Ensure sufficient cleaning of the sensors using deion. H2O after each measurement.

Sample Preparation
 Accurately weigh 15 g potato chips into a volumetric 1000 mL flask. If possible,
use plastic lab-ware (glass contains sodium & unknown interactions may happen)
especially if the samples are not analyzed immediately
 Add ambient deionized water up to the volumetric mark
 Stopper the flask and mix thoroughly by immersion

ISA solution containing 1 M Diisopropylamine, 0.36 M HCl

 Find preparation instructions on page 9 of this brochure
 The ratio of standard or sample to ISA should be 1:1.

2000 mg/L sodium in 0.5 M ISA solution

 Weigh between 5.05 – 5.10 g NaCl for a 1 Liter solution
 Dissolve in a volumetric flask using 400 mL deion. H2O
 Add 500 mL 1 M ISA
 Fill up with deion. H2O up to the 1000 mL volumetric mark
 Enter the actual concentration in mg/L of your final sodium solution (depending on
the corresponding weight of NaCl) as Standard S1 Na+, e.g 1999.88 mg/L for
5.084 g NaCl

Compound Sodium, M=22.98977 g/mol

Chemicals ISA solution: containing 1 M Diisopropylamine (DIPA), 0.36 M HCl
Na+-Standard 2000 mg/L sodium in 0.5 M ISA solution
Instruments Easy Na

Indication  ISE DX222-Na

 Reference DX205-SC
 Temperature sensor NTC 30K

Accessories Balance MS/ML (NewClassic) with USB A/B cable

USB-P25 compact printer with USB A/B cable
EasyDirectTM software

Principle Aqueous Na+ standard solution is added to the sample solution in multiple steps.

METTLER TOLEDO -1- Sodium in Food & Beverages

METTLER TOLEDO Sodium Analyzer AP201

Method Na measure Sample type solid

Standard S1: Na+ 1999.88 Sample size entry fixed weight
mg/L Sample size 15 g
No. of additions 6 Sampling Aliquot excl. ISA
Potential diff. dE 10 mV Dilution volume 1000 mL
Analysis type direct Aliquot volume 20 mL
Control normal ISA volume 20 mL
Sample ID potato chips Multiple determination Yes
Stir speed medium Calculation 1 Content [mg/kg]
Prestir duration 60 sec Calculation 2 Content [g/100g]
Report long

Sample Series Na+-content in the sample

Result R1: 0.600 g/100g
Summary R2: 0.585 g/100g
R3: 0.577 g/100g Statistics Mean = 0.587 ± 0.011 g/100g
R4: 0.576 g/100g Srel = 1.8 % (n = 5)
R5: 0.595 g/100g

Single Result Results

Sample ID Potato Chips

Content (R1) 5997.56 mg/kg
Content (R2) 0.600 g/100g
Included Yes
Sample size 14.8170 g
Sample size aliquot 0.2963 g
Volume 10.610 mL
No. of additions 6
Termination Fixed
Slope 59.7 mV/log(c)
Coefficient of determination (R²) 0.99999812

Graph

METTLER TOLEDO -2- Sodium in Food & Beverages

METTLER TOLEDO Sodium Analyzer AP201

Raw Results
Add. R1 R2 Pot. Vol. Temp.
No. [g/100g] [mg/kg] [mV] [mL] [°C]
0 -- -- 177.4 0.000 24.4
1 -- -- 187.5 0.437 24.4
2 0.577 5773.22 197.5 0.655 24.4
3 0.596 5961.68 207.6 1.019 24.4
4 0.597 5968.58 217.6 1.581 24.4
5 0.595 5954.27 227.6 2.555 24.5
6 0.600 5997.56 237.7 4.364 24.5

Waste disposal Aqueous alkaline waste

Comments  ISA solution is corrosive; use safety gloves, wear gloves and a lab coat
 The sodium content in potato chips can vary significantly and it may be necessary
to adapt the method to your specific sample. For more information, see the table
regarding the sample preparation in the theoretical part of this brochure
 Rinse the sensor with deion. water after each measurement and remove clinging
water drops with soft tissue paper
 The number of additions depends on the sample concentration and the Na+
standard concentration. For good reproducibility, the dispensed amount of sodium
standard solution should not exceed 25-35 % of the sample volume used in the
determination
 If the slope is <52 mV/log(c), the sensor should be checked and the sample
analysis repeated

Literature  Swiss Food Manual; www.slmb.bag.admin.ch

 METTLER TOLEDO Application Brochure Nr. 7 "Incremental Techniques with ISE",
ME-51724648

Author Claudia Haller, MSG AnaChem, v1.0

METTLER TOLEDO -3- Sodium in Food & Beverages