Assessment of Genetic Diversity, Antioxidant

Profiling and FC-MS Analysis of Medicinal Plants of

Euphorbiaceae (E. milli, E. hirta, E. pulcherrima)

DISSERTATION
Submitted to

SHRI GURU RAM RAI UNIVERSITY

Dehradun, Uttarakhand, India
in partial fulfillment of the requirement for the award of
degree of
MASTER OF SCIENCE
in
BIOTECHNOLOGY (2017-2019)

By
SHWETA SUNDRIYAL
Enrolment number: R170743023

Under the Supervision of

Dr. Manish Dev Sharma

Assistant Professor
Department of Biotechnology, School of Basic & Applied Sciences
Shri Guru Ram Rai University, Dehradun
(Internal Supervisor)

DEPARTMENT OF BIOTECHNOLOGY
SCHOOL OF BASIC & APPLIED SCIENCES
SHRI GURU RAM RAI UNIVERSITY, PATEL NAGAR
DEHRADUN-248001 UTTARAKHAND
 Date:

CERTIFICATE OF DEAN

This is to certify that the dissertation work entitled “Assessment of Genetic

Diversity, Antioxidant Profiling and FC-MS Analysis of Medicinal Plants
of Euphorbiaceae (E. milli, E. hirta, E. pulcherrima)” submitted by Ms
Shweta Sundriyal, Enrolment No. R170743023, has been successfully carried
out and submitted to Shri Guru Ram Rai University, Dehradun, in the partial
fulfilment of the requirement for the degree of Master of Science in
Biotechnology (2017-2019). She has worked under the supervision and
guidance of Dr. Manish Dev Sharma, Assistant Professor, Department of
Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai
University, Dehradun

I hereby forward her dissertation work.

Dr. Kumud Saklani

Dean I/C
School of Basic & Applied Sciences
Shri Guru Ram Rai University
Dehradun
 DEPARTMENT OF BIOTECHNOLOGY
SCHOOL OF BASIC AND APPLIED SCIENCES, SHRI GURU RAM
RAI UNIVERSITY, PATEL NAGAR, DEHRADUN-248001

Date:
CERTIFICATE
This is to certify that the dissertation entitled Assessment of Genetic
Diversity, Antioxidant Profiling and FC-MS Analysis of Medicinal Plants
of Euphorbiaceae (E. milli, E. hirta, E. pulcherrima)” submitted by, Shweta
Sundriyal, in the Department of Biotechnology, School of Basic and Applied
Sciences, Shri Guru Ram Rai University, Dehradun (Uttarakhand) in the partial
fulfillment of the award of the degree for the Master of Science in
Biotechnology. It is a bonafied dissertation work carried by him/her at
“Department of Biotechnology, School of Basic and Applied Sciences, Shri
Guru Ram Rai University, Patel Nagar, Dehradun"

Dr. Naveen Gaurav

Head
Department of Biotechnology
School of Basic and Applied Sciences
Shri Guru Ram Rai University
Dehradun
 DEPARTMENT OF BIOTECHNOLOGY
SCHOOL OF BASIC AND APPLIED SCIENCES, SHRI GURU RAM RAI
UNIVERSITY, PATEL NAGAR, DEHRADUN-248001

Date:

CERTIFICATE

This is to certify that Ms Shweta Sundriyal, a student of M.Sc. Biotechnology,

(IV Semester) at School of Basic & Applied Sciences, Shri Guru Ram Rai
University, Patel Nagar, Dehradun, Uttarakhand has worked on the dissertation
entitled Assessment of Genetic Diversity, Antioxidant Profiling and FC-
MS Analysis of Medicinal Plants of Euphorbiaceae (E. milli, E. hirta, E.
pulcherrima)” towards partial fulfillment of requirement for the degree of
Master of Science in Biotechnology (2017-2019). This is a faithful record of
bonafide research work done by her under my guidance. The assistance and
help received during the dissertation work and the sources of literature have
been duly acknowledged.

Dr. Manish Dev Sharma

Assistant Professor
Department of Biotechnology
School of Basic and Applied Sciences
Shri Guru Ram Rai University
Dehradun
 DECLARATION

I hereby declare that the dissertation entitled Assessment of Genetic

Diversity, Antioxidant Profiling and FC-MS Analysis of Medicinal Plants
of Euphorbiaceae (E. milli, E. hirta, E. pulcherrima)” submitted to Shri Guru
Ram Rai University, Dehradun is a faithful record of bonafide research work
carried out by me under the kind and the expert guidance as well as direct
supervision of Dr. Manish Dev Sharma, Assistant Professor, Department of
Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai
University, Dehradun. The assistance and help received during the dissertation
work and the sources of literature have been duly acknowledged. No part of
the dissertation should be extracted for any publication material without the
permission of the author and/or the supervisor.

Date: Shweta Sundriyal

Place: M.Sc. Biotechnology (2017-19)

ACKNOWLEDGEMENTS
 With all praises to god almighty who flourished me with opportunity and courage to
complete my project work with my whole sincerity and honesty.
I express my solemn gratitude to our Honourable Vice chancellor, Shri Guru Ram
Rai University, Dr. P. P. Dhyani for his moral support, positive cooperation and providing
all facilities for carrying out the entire project work. I am also indebted to Dr. Kumud
Saklani, Dean Incharge, School of Basic & Applied Sciences, Shri Guru Ram Rai
University University, Dehradun who encouraged me always and provided me necessary
help and personal care at all stages of my research work.
I feel highly grateful to Dr. Naveen Gaurav, Head, Department of Biotechnology,
School of Basic & Applied Sciences, Shri Guru Ram Rai University, Dehradun for his help
and encouragement during my dissertation which helped me to complete this research work
with great ease. I feel immense pleasure in expressing my deep gratitude and thanks towards
my supervisor Dr. Manish Dev Sharma Assistant Professor, Department of Biotechnology,
School of Basic & Applied Sciences, Shri Guru Ram Rai University, Dehradun. Their
constant help, support, complete involvement and encouragement lead me to complete my
training and gain preliminary knowledge of research and management of time and resources.
I want to express my deepest gratitude and thanks to my all teachers of School of
Basic & Applied Sciences, Shri Guru Ram Rai University, Dehradun for giving me their
constant help and support.
I am also very thankful to all non- teaching staff for providing guidance, support and
constant encouragement whenever I needed.
Lastly I also express my indebtedness to my parents and my friends whose support,
persistent prayers, love and affection has been a source of encouragement which shall
always motivate me to move ahead in my life.

Shweta Sundriyal Date:

M.Sc. Biotechnology (IV Sem) Place: Dehradun

TABLE OF CONTENTS
 Contents Page No.

Chapter 1: INTRODUCTION

OBJECTIVES

Chapter 2: REVIEW OF LITERATURE

Chapter 3: MATERIALS & METHODS

Chapter 4: RESULTS AND DISCUSSION

Chapter 5: CONCLUSION

Chapter 6: REFERENCES

ABBREVIATIONS