Assignment lab

Concentration (mg/mL) Peak height (Arbitrary units)

0 24.79
0.0125 46.77
0.0482 80.40

Peak height VS Concentration

90
Peak Height (Arbitrary units)

80
f(x)==0.97
R² 1102.17 x + 28.52
70
60
50
40
30
20
10
0
0 0.01 0.02 0.03 0.04 0.05 0.06

Concentration (mg/mL)

Concentration (mg/mL) Peak Area (Arbitrary units)

0 408.3
0.0125 831.7
0.0482 1278.6

Peak Area VS Concentraion

1400
Peak Area (Arbitrary units)

1200 f(x)==0.93
R² 16742.1 x + 503.3

1000

800

600

400

200

0
0 0.01 0.02 0.03 0.04 0.05 0.06

Concentration
From the graph peak height vs concentration, using the equation Y=mx+c to determine the
original concentration.

y=1102.2 x +28.518
To find the original concentration, the value of peak height at 0 concentration of standard
caffeine was used in this calculation which is 24.79.

24.79=1102.2 x +28.518
x=−0.00338 mg/mL
The value -0.00338 is for after 50 mL dilution concentration. So, the original concentration is

original concentration=−0.00338× 10=−0.0338 mg/ mL

Therefore, to calculate original concentration for peak area will use the same calculation.

y=16742 x+ 503.3
408.3=16742 x +503.3
x=−0.00567 mg/mL
original concentration=−0.00567× 10=−0.0567 mg/mL