TUTORIAL 1

(CHAPTER 1: Introduction to Spectroscopic Method of Analysis)

1. Arrange the following electromagnetic spectral regions in order of increasing energy.

Visible Ultraviolet Infrared Microwave X-ray

2. A molecular absorption band appears at a wavenumber of 712 cm-1. Calculate;

a) the wavelength (in m) of the radiation.

b) the energy (in Joules) of this radiation.

3. A 6.00 × 10-5 solution of KSCN is put into a sample cuvette with a path length of 1.2 cm.
The absorbance at 650 nm is found to be 0.436. An unknown solution of the KSCN has
an absorbance of 0.638 when analyzed under the same experimental condition.
Calculate the concentration of the unknown solution.

4. Identify factors that cause the Beer’s law relationship to depart from linearity.

5. A solution containing 4.48 ppm KMnO 4 has a transmittance of 0.309 in a 1.00 cm cell at
520 nm. Calculate the molar absorptivity of KMnO 4.

6. A 25.6 mg sample of a compound with a molecular weight of 317.17 g/mol was

dissolved with the solvent in a 300-mL volumetric flask. The absorbance of this solution
at 238 nm was 0.624 in a 1.00 cm cuvette. Calculate the molar absorptivity of this
compound.