- Major: Pre-Pharmacy

- Title: Using The Microscope

- Date: 0ctober 12, 2012

I. Introduction :
A microscope contains lenses, which focus
radiation emanating from a specimen to
produce an image of that specimen and on
a surface sensitive to the radiation.
The microscope composed of different parts
which are: Ocular, arm, nosepiece,
objective, mechanical stage, fine
adjustment, coarse adjustment, condenser
adjustment knob, condenser, base
illuminator and lever for iris diaphragm.

II. Objectives :
- Discover the main parts of the
microscope and their functions.
- Making practical experiments.

III. Achieving goals :

o - Condenser lens: focus light rays onto
specimen so that each of its points is
evenly illuminated.
- Iris diaphragm: it is used to open and
close the condenser.
- Objective: form the primary image, it
scans the specimen.
 - Ocular: enlarges primary image
formed by objective lenses.

o The lenses in the light path between

specimens viewed with the compound
light microscope and its image on the
retina of the eye are:
Condenser lensesobjective lensesocular
lenses.
o When the numerical aperture of the
condenser of a compound light
microscope is decreased, the contrast
and resolving power become lesser
and not useful magnification.

o When the total magnification is

increased, the field of view decreases
dramatically
o The importance of the following part
of the microscope is:
- Magnification: to enlarge the image
of an object.
- Resolving power: to see the extent to
which object detail in an image is
preserved during magnification.
- Contrast: to see the degree of
preserving details in an image under
magnification.