Experimental:

Firstly, we set the values on the GC after the instructions from the instructor. Then we
set initial and final column temperature which is 100 celcius. Then, we injected 0.4 µL of the
alcohol mixture onto column A. We injected each alcohol individually, 2-propanol followed
by n-butanol as much air as possible. We adjust parameters as necessary until retention times
for each peak can be analyzed. Then, the retention time and the areas of each component are
recorded. We obtained 3 chromatograms with good reproducibility of the retention times and
peak areas of each component.
Secondly, we injected the standard mixture and each component present is identified by
comparing the retention time of each component with the retention time of each single
component determined previously. Then we injected the sample with unknown quantity and
each component is identified by obtain at least 3 injections.
Next step, we changed the column temperature to 80 celcius and injected the standard
mixture. After that, we changed the column temperature to 130 celcius than injected the
standard mixture again. We compared the chromatograms of the standard mixtures using 3
different column and commented on the effect of column temperature on retention times,
resolution and the peak areas. Last but not least, we injected the standard mixtures and
quantitative analysis of the sample should be done using the area percent and external
standard methods.