Natufactant QnA19042017 Bang PDF

Bioproton Pty Ltd ABN 19 059 093 417
NATUFACTANT GUIDE
Due to rising feed costs -‐ pressure is growing to use ingredients that are cheaper, which however have lower
energy content. This can lead to an imbalanced feed energy content causing lower feed efficiency. Lipids in the
form of oils and fats can be incorporated into diets to improve energy content of the feed. However lipids are
an expensive macro ingredient so for economical reasons their bio-‐availability needs to be maximised,
Nutritional bio-‐surfactants like Natufactant can be used to improve lipid digestion.
1. Differences between Emulsifier, Surfactant and Biosurfactant
• Technical Emulsifier: Are a type of emulsifiers, which improve water retention during feed production.
This ensures optimal humidity levels are obtained for hygiene, and energy usage is reduced due to less
grinding -‐ delivering a smoother production run. A HLB (Hydrophilic Lipophilic Balance, see definition
below) value of 5 -‐7 is optimal for these types of emulsifiers. These technical emulsifiers do not improve
the digestion of lipids in a farm animal. These emulsifiers are usually petroleum based, i.e. synthetic.
• Nutritional Surfactant: Are a surfactant that breaks down lipids in animal feeds into smaller molecules
and therefore making them more digestible, resulting in improved energy intake, which improves feed
efficiency. Typically a high 10 – 15 HLB value is desirable. This type of surfactant can be made from
synthetic or from organic sources (see Nutritional Bio-‐Surfactant Definition).
• Nutritional Bio-‐surfactant: in the feed context: is produced from organic plant based sources, where as
surfactant typically is made from Petroleum or from synthetic sources.
• Lipids: any fat (animal) or oil (cereal) based components that are added into feed to boost feed energy
content.
2. Criteria for Choosing Nutritional bio-‐Surfactant
The criteria for choosing an optimal nutritional bio-‐surfactant to improve lipid digestion are a) HLB value, b) CMC
(Critical Micelle Formation) and c) Micelle Droplet Size
a) HLB Value
HLB Value means Hydrophilic (the water soluble part) –Lipophilic (fat soluble part) Balance. This shows how
water soluble or fat soluble a product is. HLB values ranges from 0 to 20. The lower the HLB, the more fat-‐
soluble the emulsifier becomes and the higher the HLB, the more water-‐soluble the emulsifier will be.
An emulsifier should be soluble in the continuous phase (Bancroft Rule). This means that a lower HLB value is
advised when mixing a small amount of water into a fat-‐rich environment. And an emulsifier with higher HLB
value is advised if a small amount of fat is mixed into an aqueous or watery environment. (Rovers)
55 Dulacca Street, Acacia Ridge, Qld 4110, Australia

T:+61 7 3344 1490| F:+61 7 3345 9582 | E:info@bioproton.com | www.bioproton.com
Natufactant QnA19042017 Bang.doc Page 1 of 7
<10 oil soluble
>10 water soluble
4-‐8 anti foaming agent
7-‐11 water-‐in-‐oil emulsifier
12-‐16 oil-‐in-‐water emulsifier
11-‐14 wetting agent
12-‐15 detergent
16-‐20 stabilizer
Table 1. HLB Values and Properties
According to research done by Bioproton -‐ the HLB value of Natufactant is between 10 – 14 (in the range of
higher HLB value). Lysolecithin (active component of Lysoforte) is 8 -‐ 12; surfactants on this HLB range are called
nutritional surfactants. See table 2.
HLB Value
Natufactant 10 -‐ 14
Lecithin 4 -‐ 10
Lysolecithin (active component of Lysoforte) 8 -‐ 12
Bile Acid >18
Technical Emulsifiers < 5
Table 2. Typical HLB values
In a practical application, limited amount of fat is represented in animal feeds relatively to water. For poultry
and pigs water intake is almost 2x more than their feed intake. And so, a high HLB value is more appropriate in
this case. Natufactant with an HLB value of 10 – 14 is optimally targeted to ensure it complement natural bile
acids to provide an optimal lipid breakdown and digestion.
Some synthetic nutritional emulsifiers have HLB value of >18, however this competes with Bile Acids, who
already provide an effect in this range.

Hydrolysed Pisum Protein – wetting effect.
In Natufactant – there is also small concentration of “Pisum protein” that is able to provide a so-‐called “wetting”
effect; this provides a unique characteristic compared to other emulsifiers. At this lower HLB
region hydrophobic digestive enzymes in the gut are able to achieve a greater intimacy with feed substrates that
are considered to have an anti-‐nutritional effect. This improves nutritional uptake from feed and results in
improved FCR. Note that this improves the effectiveness of digestive enzymes (irrespective if the enzymes are
endogenously or exogenously added to feed).
b) CMC (Critical Micelle Concentration)
CMC is the measure of an emulsifier or surfactants efficiency. Lower CMC indicates that less surfactant or
emulsifier is needed to saturate interfaces and form micelles.
Natufactant has both hydrophobic scaffold & several hydrophilic oligosaccharide chains enabling a very low
critical micelle concentration (CMC) and good fat emulsification.
• Micelle – an aggregated unit composed of number of molecules of a surface active material
• Micelle Formation – enables emulsification, solubilization and dispersion
• CMC – is the concentration at which appreciable micelles are formed.
CMC (g/L)
Natufactant <0.5
Lecithin <1
Lysolecithin (active component of Lysoforte) <1
Bile Acid <0.5
Table 3. CMC (Critical Micelle Concentration)

c) Micelle Droplet Size
A smaller micelle, leads to greater surface area for the micelle droplet size. This leads to an improved
absorption and digestion of the micelle in the digestive system of the animal.
Please take note that based on a study done by Bioproton, Natufactant leads to much smaller micellar size (3.8
nm) as compared to Lysolecithin (7.2 nm). The smaller the droplet size, the more surface area is made available
for absorption – leading to improved energy uptake.
Micellar Size (nm)
Natufactant 3.8
Lecithin 25
Lysolecithin (active component of Lysoforte) 7.2
Bile Acid 1.5
Table 4. Micellar Size

3. Energy Reductions
The energy reductions that Nutritional Surfactant / Biosurfactant can deliver also depend on the lipid source,
and how bioavailable these are already. The factors that govern this are a) Fatty acid chain length, b) Degree of
saturation and c) Ratio of unsaturated and saturated fats of different. E.g. Rice Bran Oil will respond better to an
emulsifier than Palm Oil because of these factors. Soy Oil is considered being highly digestible, so an extra 10-‐
20% energy uplift can be achieved with Palm Oil, Coconut Oil compared to Soy Oil.
In addition the age / type of species can also affect the energy reductions. These are summarised in the table
below.
Energy Reductions for kg per ton from a dose of Natufactant at 250g/t
Broiler Broiler Layer

Lipid Type
Starter Feeds Finisher / Grower Feed
Sunflower Oil 5.00 4.50 4.50
Soybean Oil 5.00 4.50 4.50
Rice Bran Oil 5.00 4.50 4.50
Palm Oil 6.00 5.0 5.0
Tallow 6.00 5.0 5.0
Lard 6.00 5.0 5.0
Fish Oil 6.00 5.0 5.0
Coconut Oil 5.50 4.50 4.5
Table 5 Ingredient reductions (kg per ton)

4. Thermostability
The active ingredient in Natufactant is Quillaja saponaria. This compound is highly stable and has a
decomposition temperature of >150 Degree Celsius, which is significantly above industry feed pelleting
temperatures.
Bioproton has undertaken a number of laboratory trials were samples of Natufactant have been subjected to
high temperatures between 65 – 100 Degree Celsius, with no changes to product structure and no lipid
emulsification was observed.
In addition, Natufactant has been used in 3 broiler bird field trials between 2014, 2015, and 2016 with >85
Degree Celsius pelleting temperatures. In these trials broilers that were administered Natufactant
demonstrated significantly higher feed efficiency and weight gain, therefore demonstrating improved energy
uptake via improved lipid digestion.
Natufactant can be used successfully in all feed pelleting operations.
5. Summary
In summary, our product, NATUFACTANT, is a BIO-‐SURFACTANT ENERGY BOOSTER. With its high relatively
high, but also broad spectrum HLB values, it is soluble in the continuous phase such as inside the gut of the
animal. Not only this, it provides very low critical micelle concentration which indicates that you only need small
amount of Natufactant to stabilize the interfaces and to form emulsion droplets at nano-‐scale. It will therefore
provide good fat emulsification and fat hydrolysis thus improving absorption of energy from fats and oils
incorporated in the feeds.
Bioproton can recommend fats/oils substitution, energy reduction or using it as an add-‐on for energy boosting
effect provided that the energy values and feed formulations will still maintain and meet the specific energy
requirement of the animal.
6. Why Is Natufactant Is Good Bio-‐Surfactant
1) Optimal HLB values:

a) Complements a watery gut environment
b) Does not compete with Bile Acids
c) Hydrolysed Pea Proteins provide a unique wetting agent that optimises digestive enzyme function.
2) Natural ingredients, all ingredients in Natufactant are naturally derived and non-‐synthetic.
3) Low CMC, which provides good efficacy
4) Leads to smaller emulsion droplets, which increases surface area – leading to improved bio-‐availability
5) Produced under a GMP System to ensure quality, safety and consistency.

7. Glossary
Surfactant: Surfactant molecules, defined as surface-‐active agents, consist of a hydrophobic and a hydrophilic
moiety that are clearly separated in the molecular structure. Surfactant molecule lowers the interfacial tension
between the oil and water phases.
Emulsifier: An emulsifier is a type of surfactant typically used to keep emulsion (mixtures of immiscible fluids)
well dispersed. Oil and water can form two types of emulsions. First, an oil-‐in-‐water emulsion, wherein the oil is
the dispersed phase, and water is the dispersion medium. Second, a water-‐in-‐oil emulsion, wherein water is the
dispersed phase and oil is the external phase. An emulsifier stabilizes the emulsion by reducing the interfacial
tension, creating repulsion or modify viscosity of the system.
Technical Emulsifier: Are a type of emulsifiers, which form water-‐in-‐oil emulsion and improve water retention
during feed production. This ensures optimal humidity levels are obtained for hygiene and energy usage is
reduced due to less grinding delivering a smoother production run. A HLB value of 5 -‐7 is optimal for these types
of emulsifiers. These technical emulsifiers do not improve the digestion of lipids in a farm animal. These
emulsifiers are usually petroleum based, i.e. synthetic.
Nutritional Surfactant: Are surfactant that breaks down lipids in animal feeds into smaller molecules, they form
oil-‐in-‐water emulsion and therefore making them more digestible, resulting in improved energy intake, which
improves feed efficiency. Typically a high 10 – 15 HLB value is desirable. This type of surfactant can be made
from synthetic or from organic sources (see Nutritional Bio-‐Surfactant Definition).
Nutritional Bio-‐surfactant: in the feed context: is produced from organic plant based sources, where as
surfactant typically is made from Petroleum or from synthetic sources.
Lipids: any fat (animal) or oil (cereal) based components that are added into feed to boost feed energy content.


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Bioproton Pty Ltd ABN 19 059 093 417

1. Differences between Emulsifier, Surfactant and Biosurfactant

2. Criteria for Choosing Nutritional bio-­‐Surfactant

55 Dulacca Street, Acacia Ridge, Qld 4110, Australia

<10 oil soluble

>10 water soluble

4-­‐8 anti foaming agent

7-­‐11 water-­‐in-­‐oil emulsifier

12-­‐16 oil-­‐in-­‐water emulsifier

11-­‐14 wetting agent

Table 1. HLB Values and Properties

Natufactant 10 -­‐ 14

Lecithin 4 -­‐ 10

Lysolecithin (active component of Lysoforte) 8 -­‐ 12

Bile Acid >18

Technical Emulsifiers < 5

Table 2. Typical HLB values

55 Dulacca Street, Acacia Ridge, Qld 4110, Australia

Hydrolysed Pisum Protein – wetting effect.

b) CMC (Critical Micelle Concentration)

Lysolecithin (active component of Lysoforte) <1

Bile Acid <0.5

Table 3. CMC (Critical Micelle Concentration)

55 Dulacca Street, Acacia Ridge, Qld 4110, Australia

c) Micelle Droplet Size

Micellar Size (nm)

Lysolecithin (active component of Lysoforte) 7.2

Bile Acid 1.5

Table 4. Micellar Size

55 Dulacca Street, Acacia Ridge, Qld 4110, Australia

Broiler Broiler Layer

Sunflower Oil 5.00 4.50 4.50

Soybean Oil 5.00 4.50 4.50

Rice Bran Oil 5.00 4.50 4.50

Palm Oil 6.00 5.0 5.0

Tallow 6.00 5.0 5.0

Lard 6.00 5.0 5.0

Fish Oil 6.00 5.0 5.0

Coconut Oil 5.50 4.50 4.5

Table 5 Ingredient reductions (kg per ton)

55 Dulacca Street, Acacia Ridge, Qld 4110, Australia

6. Why Is Natufactant Is Good Bio-­‐Surfactant

1) Optimal HLB values:

55 Dulacca Street, Acacia Ridge, Qld 4110, Australia

55 Dulacca Street, Acacia Ridge, Qld 4110, Australia

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2. Criteria for Choosing Nutritional bio-‐Surfactant

4-‐8 anti foaming agent

7-‐11 water-‐in-‐oil emulsifier

12-‐16 oil-‐in-‐water emulsifier

11-‐14 wetting agent

Natufactant 10 -‐ 14

Lecithin 4 -‐ 10

Lysolecithin (active component of Lysoforte) 8 -‐ 12

6. Why Is Natufactant Is Good Bio-‐Surfactant