AS Biology

BYB1 Core Principles

10.4 Enzymes Test

Name

Date

Question Marks

1 /19

2 /5

3 /4

4 /3
5
/2
6
/4
7
/2
8
/4
9
/2
Total
/45
%

Grade

A = 36/45 (80%) B = 32/45 (70%) C = 27/45 (60%) D = 23/45 (50%) E = 18/25 (40%)
Enzyme Properties

1. Complete the following paragraphs filling in the missing words (19 marks)

Enzymes are biological catalysts, reducing the energy of reactions. They are

composed of proteins with a specific tertiary structure.

In the hypothesis, the substrate shape is

complementary to the shape of the active site. In the hypothesis the

active site alters to fit the substrate.

Increasing temperature increases the energy of the enzyme and substrate

molecules, with high temperature causing the enzyme to become irreversibly .

Enzymes operate in a pH range, with changing pH around the enzyme disrupting

the .

Increasing enzyme concentration causes a increase in the rate of an enzyme-

catalysed reaction, whereas increasing substrate concentration also increases rate but only

until a is reached where all active sites are .

Some of the words in this box may be useful.

Activation Denatured Enzyme Fibrous

Globular Indirect Induced fit Kinetic
Linear Lock and key Maximum rate Minimum rate
Narrow Potential Saturated Shape of the active site
Shape of the substrate Substrate Unsaturated Wide
2. Draw a labelled sketch graph to show how temperature affects enzyme activity
(5 marks)

3. Explain in your own words, with a scientific reason, why this curve shape is
produced (4 marks)

4. Draw a labelled sketch graph to show how pH affects enzyme activity (3 marks)

5. Explain in your own words, with a scientific reason, why this curve shape is
produced. (2 marks)
6. Draw a labelled sketch graph to show how substrate concentration affects
enzyme activity. (4
marks)

7. Explain in your own words, with a scientific reason, why this curve shape is
produced. (2 marks)

8. Draw a labelled sketch graph to show how enzyme concentration affects enzyme
activity. (4 marks)

9. Explain in your own words, with a scientific reason, why this curve shape is
produced (2 marks)
 (Maximum 45 marks)
Mark scheme
1.) All words entered correctly (max 19)
3.) Increased initial rate of reaction
2.) (max 5) due to higher temperature
1 causing more collisions (1)

Plateau due to optimum

Enzyme temperature (1)
activity 1 1
1 Fall due to excess temperatures
causing enzymes to disintegrate
and shape of active site lost (1)
- becoming denatured (1)
Temperature 1 (max 4)

4.) (max 3)
5.) Bell shaped curve - optimum pH
1 in the centre (1)

Lower at both sides due to active

Enzyme site shape changed due to charge
activity 1 (1)
(max 2)

pH 1
7.) Increased initial rate due to
6.) (max 4)
more substrate available to
1 collide with enzymes (1)

Enzyme Plateau due to all active sites

1 being filled (1)
activity 1
(max 2)

Substrate concentration 1

9.) Increased initial rate due to

1 more enzymes available to
8.) (max 4)
collide with substrate (1)
Enzyme 1
activity 1 Plateau due to all active sites
being filled (1)
(max 2)

(Max 45)
Enzyme concentration 1

A = 36/45 (80%) B = 32/45 (70%) C = 27/45 (60%) D = 23/45 (50%) E = 18/25 (40%)