Page

e 1 of 2

eBiosc
cience™ Phytohem
magglutinin-L (PH
HA-L) Sollution (50
00X)
4977 
Catalog Number: 00-4
N
For Rese
earch Use On
nly. Not for use in diagno
ostic procedu
ures.
 

Le ft: Human peripheral blood m mononuclear ce ells

weere labeled withh 10 uM Cell Proliferation Dyee
eFFluor® 450 (catt. 65-0842) andd cultured for 3 days
witth Phytohemag gglutinin-L (PHAA-L) Solution (500X)
at 2 uL per mL off culture mediuum. Cells were
sta
ained with Anti--Human CD86 PE (cat. 12-08 869)
an d Fixable Viabbility Dye eFluor® 660 (cat. 655-
08 64). Total singlet-gated, viable cells were ussed
forr analysis.

ght: Mouse splenocytes were

Rig e labeled with 1
10 uM
Ceell Proliferation Dye eFluor® 4 450 (cat. 65-08
842)
an d cultured for 3 days with Phyytohemagglutin nin-L
(PHHA-L) Solution n (500X) at 2 uLL per mL of cullture
meedium. Cells we ere stained witth Anti-Mouse CCD86
PEE-Cyanine7 (ca at. 25-0862) and Fixable Viability
Dyye eFluor® 660 0 (cat. 65-0864). Total singlet--
ga ted, viable cells were used foor analysis.

Product In
nformation
  science™
Contents: eBios
C   Formulation: Sterile aqueou
us buffer, no so
odium
Phytohemagglu
P tinin-L (PHA-L)) Solution a
azide
(5
500X)  T
Temperature Limitation: Stoore at -20°C.
  Catalog
C Numbe er: 00-4977       
  Batch Code: RRefer to vial 
   Concentration:
C : 500X (1.25 mg/mL)
  Handling
H ditions: Use in sterile
Cond   Use By: Referr to vial
environment.  
 
  Source:
S Phaseoolus vulgaris

Descriptio on
The Phytoh hemagglutinin--L (PHA-L) Solu ution (500X) is a ready to use e solution of PH
HA-L in aqueou us buffer. PHA is a
mixture of lectins isolated d from the red kidney
k bean, Phaseolus
P vulgaaris. PHA-L is the fraction of PHA that exhib bits
strong leukkoagglutinating and mitogenic c activity, but ve oagglutinating activity. This re
ery little erythro eagent is intend
ded
for use in in
n vitro activatio
on of human an nd mouse leuko ocytes.

ons Reported
Applicatio
Phytohemaagglutinin-L (PH
HA-L) Solution (500X) has be
een reported fo
or use in in vitro
o cultures.

Applicatio ons Tested

The activity y of the Phytohhemagglutinin-LL (PHA-L) Solu
ution (500X) ha as been tested by proliferation n of mouse
splenocyte es or human pe eripheral blood mononuclear cells
c as measu ured by dilution of Cell Proliferration Dye eFlu
uor®
450. This is s a pre-titrated 500X solution and can be dilluted to 2 μL pe e medium. This reagent may be
er mL of culture
further-titra
ated for optimal performance in the assay off interest.

Under the testing

t conditio
ons listed above, no change in performance
e is observed affter 20 freeze-tthaw cycles. Fo
or
optimal perrformance, smaaller aliquots may
m be prepareed to minimize tthe number of freeze-thaw cyycles.

Reference es
Lai-Hipp C, Goldberg T, Scott
S E, Ziman A, Vyas G. Poooled peripheraal blood mononnuclear cells prrovide an optimmized
cellular sub
bstrate for hum
man immunodefficiency virus Type
T 1 isolation
n during acute iinfection. Transsfusion. 2011
Feb;51(2):3 333-7.

Not for further

f distrib
bution withoutt written conseent.
Copyrightt © 2016 Thermo Fisher Scientific Inc. All
A rights rese erved. All trademarks are pproperty of Th
hermo
Fisher Scientiffic and its subbsidiaries unle
ess otherwise e specified.
Tel: 888.999.1371
8 1 or 858.642.2 2058 • Fax x: 858.642.204 46 • thermo ebioscience •
ofisher.com/e
info@e ebioscience.co om
14 March 2017 Rev. 100
 Page
e 2 of 2

eBiosc
cience™ Phytohem
magglutinin-L (PH
HA-L) Sollution (50
00X)
4977 
Catalog Number: 00-4
N
For Rese
earch Use On
nly. Not for use in diagno
ostic procedu
ures.
 

k TW, Dao-Thi MH,

Hamelryck M Poortmans
s F, Chrispeels
s MJ, Wyns L, Loris R. The crrystallographic structure of
phytohema
agglutinin-L. J Biol
B Chem. 199
96 Aug 23;271((34):20479-85..

Yachnin S,, Svenson RH. The immunoloogical and phys

sicochemical prroperties of mittogenic protein
ns derived from
m
Phaseolus vulgaris. Immu
unology. 1972 May;22(5):871-83.

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Not for further

f distrib
bution withoutt written conseent.
Copyrightt © 2016 Thermo Fisher Scientific Inc. All
A rights rese erved. All trademarks are pproperty of Th
hermo
Fisher Scientiffic and its subbsidiaries unle
ess otherwise e specified.
Tel: 888.999.1371
8 1 or 858.642.2 2058 • Fax x: 858.642.204 46 • thermo ebioscience •
ofisher.com/e
info@e ebioscience.co om
14 March 2017 Rev. 100