TCR alpha antibody (pAb)

08.12.2015

Rabbit Anti-Human T-cell Antigen Receptor alpha

Instruction Manual

Catalog Number PK-AB577-5648

Description TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events
into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is
a heterodimer composed of either α and β or γ and δ chains. The vast majority of circulating T cells
(95%) express the α/β heterodimer while roughly 2-5% express the γ/δ heterodimer. CD3 chains
and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the
TCR.
Quantity 100 µg
Source / Host Rabbit
Immunogen Synthetic peptide surrounding amino acid 138 of human TCRα (ID BV-417).
Clone / IgG Subtype Polyclonal antibody
Species Reactivity Human
Specificity The antibody recognizes TCRα in human sample, and to a lesser extent to mouse and rat samples.
Formulation 100 µg (0.2 mg/ml) immunoaffinity purified rabbit polyclonal antibody in phosphate-buffered
saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Reconstitution Can be diluted in other aqueous buffers at the concentrations determined for the respective
application.
Storage & Stability Store at -20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost
cycles.
Applications The antibody can be used in Western blotting (0.5-4 µg/ml). Based on researcher’s feedback, it
can also be used in immunoprecipitation (10-20 µg/ml); and immunohistochemistry (15-25
µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate
(Cat.# PK-AB718-1205) can be used as a positive control. Blocking peptide is available separately
(Cat.# PK-AB577-5648P).

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

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