PROJECT REPORT

On
“Assessment of Physico-chemical parameters and Microbiological analysis of
ground water of Bakshi Ka Talab, Lucknow”

submitted in partial fulfillment of the requirement for the award of degree of

Master of Science
in
Industrial Chemistry
SESSION: 2017-2018

Submitted by
KAVYA SRIVASTAVA

Enrollment No - 1300102201

Department of Chemistry
Integral University, Lucknow-226026
 DECLARATION
I, hereby declare that the project titled: “ASSESSMENT OF PHYSICO-CHEMICAL
PARAMETERS AND MICROBIOLOGICAL ANALYSIS OF GROUND WATER OF
BKT, LUCKNOW” is a record of an original work done by me during my 3 months
training, from Feb 2018 to May 2018 at Sishodia Research Laboratories Pvt. Ltd The
training has been carried out under the guidance of Dr. V.K. Awasthi.

Kavya Srivastava
 ACKNOWLEDGEMENT

First of all I am grateful to Almighty God for helping me to complete my thesis.

I wish to express my sincere thanks to Dr. P.N. Singh Sishodia who provide me great

opportunity for conducting my work smoothly.

I owe my deep sense of gratitude to, Dr. V. K Awasthi, Rtd. Scientist IITR, Lucknow and

Prof. Dr. Abdul Rahman Khan (Head Of Department), Integral University Lucknow for

being highly supportive and encouraging during the whole tenure of the training.

I would like to express my sincere thanks to Dr. Naseem Ahmad Assistant professor

Integral University Lucknow and all the faculty members of chemistry department of Integral

University Lucknow.

Last but not the least I would to express my heartly thanks to Dr. Aisha Siddique without

whose support, guidance and encouragement it would have been impossible to complete the

present work.

At the same time I would like to thank my friend Nazneen Kauser (my project partner) and

also my friends Salma Khan, Surbhi Mathur, Md. Qasim, Waseem Ahmad, Om

Prakash, Jay Prakash, Adarsh Kumar Verma for their passionate encouragement and

moral support extended with love and great patience and constant source of motivation as

always which made it possible for me to complete my work.

I must make special mention of my parents, my sister, and my brother for their blessings,

motivation patience and without their co-operation, this project would not have been

accomplished.
 ABBREVIATIONS

BKT: Bakshi Ka Talab

L: Litre

ml: mili litre

MPN: Most probable number

mmhos: Millimhos

EDTA: Ethylene diamine tetra acetate

EBT: EriochromeblackT

EC: Electrical Conductivity

TDS: Total Dissolved Solids

TSS: Total Suspended solids

BIS: Bureau Of Indian Standard

WHO: World health Organisation

 TABLE OF CONTENT

S.No. CONTENT Page No.

1 INTRODUCTION 2-4

2 REVIEW OF LITERATURE 6 - 10

3 OBJECTIVES 12 - 13

5 MATERIAL & METHODS 15

6 INSTRUMENTATION 17 - 31

7 RESULT AND DISCUSSION 33 - 45

8 CONCLUSION 46

9 REFERENCES 47 – 48
 LIST OF FIGURES

S.No. Title Page No.

1 Figure used to show definition for ground water 3

2 Graphical representation of grond water associated 3

term

3 Category of ground water assessment unit 19

4 Site location 12

5 Sampling site 15

6 pH meter 17

7 UV-Visible Spectrophotometer 18

8 Magnetic Stirrer 18

9 Figure of hardness present 21

10 Figure of calcium present 21

11 Preparation of media sterilisation 30

12 Sample placed in incubator 31

13 Graph showing pH 34

14 Graph showing EC 35

15 Graph showing Turbidity 35

16 Graph showing Total Hardness 36

17 Graph showing Calcium 36

18 Graph showing Magnesium 37

19 Graph showing Alkalinity 37

20 Graph showing Chloride 37

21 Graph showing Sulphate 38

22 Graph showing Nitrate 39

23 Graph showing Iron 39

24 Graph showing TSS 40

25 Graph showing TDS 40