Hypospadias and Early Gestation Growth Restriction in Infants

Naveed Hussain, Azhar Chaghtai, C. D. Anthony Herndon, Victor C. Herson, Ted S.

Rosenkrantz and Patrick H. McKenna
Pediatrics 2002;109;473-478
DOI: 10.1542/peds.109.3.473

The online version of this article, along with updated information and services, is
located on the World Wide Web at:
http://www.pediatrics.org/cgi/content/full/109/3/473

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 Hypospadias and Early Gestation Growth Restriction in Infants
Naveed Hussain, MBBS*; Azhar Chaghtai, MD*; C. D. Anthony Herndon, MD‡; Victor C. Herson, MD*;
Ted S. Rosenkrantz, MD*; and Patrick H. McKenna, MD‡
ABSTRACT. Objective. There has been a major in-
crease in the incidence of hypospadias in infants in the
1990s, but the risk factors are not known. Although there
are scattered reports in the literature regarding the asso-
ciation of low birth weight and hypospadias, this has not
been systematically studied. The objective of this study
was to determine the association between early gestation
intrauterine growth and hypospadias.
Methods. A retrospective review of 13 years of admis-
sions to 2 tertiary care neonatal intensive care units (NI-
CUs) in Connecticut (1987–2000) showed that 112 (1.66%)
of 6746 male infants had any degree of hypospadias. Of
these, 8 were part of a genetic syndrome and were ex-
cluded. A retrospective cohort analysis of these 6738 in-
fants was performed. Infant growth parameters at birth
(weight, head circumference, and length) were analyzed
along with maternal risk factors known to be associated
with changes in fetal growth, including maternal age,
race, diagnosis of preeclampsia, gestational diabetes, and
maternal use of alcohol or tobacco or substance abuse
during pregnancy.
Results. The incidence of hypospadias in the NICU
population increased 10-fold from 0.4% in 1987 to 4% in
the first quarter of 2000. Hypospadias was significantly
more common in infants who had uniformly poor intra-
uterine growth (<10th percentiles) in the various param-
eters measured: birth weight, length, or head circumfer-
ence. There were no significant differences in maternal
age or race, nor were there differences in the use of
alcohol, tobacco, or street drugs by the mother. There
were no differences between singletons and multiple-
gestation births. However, the frequency of occurrence
was significantly higher among first-born infants (1.9%)
compared with all other infants (0.9%).
Conclusions. The incidence of hypospadias in our
NICU population has increased 10-fold during the 13-
year period of study. There was a significant association
of hypospadias with poor intrauterine growth. The
growth restriction was probably of early gestational
cause as there was proportionate involvement of somatic
(weight and length) and brain growth (head circumfer-
ence). The increasing frequency of hypospadias and its
association with poor intrauterine growth originating in
early gestation suggests that common environmental fac-
tor(s) that have an impact on both conditions may be
From the Division of Neonatology, *Department of Pediatrics, University
of Connecticut Health Center, Farmington, Connecticut; and ‡Division of
Urology, Department of Surgery, Connecticut Children’s Medical Center,
Hartford, Connecticut.
This study was presented, in part, as a platform presentation at the Society
for Pediatric Research Meeting; May 12–16, 2000; Boston, MA.
Received for publication Sep 29, 2000; accepted Aug 23, 2001.
Reprint requests to (N.H.) University of Connecticut School of Medicine,
Farmington, CT 06030-2948. E-mail: hussain@nso1.uchc.edu
PEDIATRICS (ISSN 0031 4005). Copyright © 2002 by the American Acad-
emy of Pediatrics.
Downloaded from www.pediatrics.org at University of Connecticut on February 9, 2009
involved. Pediatrics 2002;109:473– 478; hypospadias, SGA,
intrauterine growth, temporal trend, risk factors.
ABBREVIATIONS. SGA, small for gestational age; AGA, appro-
priate for gestational age; LGA, large for gestational age; NICU,
neonatal intensive care unit; UCHC, University of Connecticut
Health Center; GA, gestational age; BW, birth weight; SEM, stan-
dard error of the mean; EDC, endocrine disrupter chemicals.
T
he development of a normal urethra in males is
a complex process, and factors that lead to its
abnormal development, resulting in hypospa-
dias, are largely unknown.1 Hypospadias is one of
the most common congenital anomalies in males.2
Reports from Europe and the United States have
shown an increasing incidence of hypospadias from
1970 to the present.3,4
The risk factors for hypospadias are not well un-
derstood. An association between low birth weight
and hypospadias has been made,4 – 6 but a systematic
analysis of the association between hypospadias and
growth restriction has not been conducted. It is also
not clear whether the low birth weight is attributable
to a lower gestational age or intrauterine growth
restriction.5,6 With currently available norms for in-
trauterine growth to classify even the most immature
infants into small, appropriate, or large for gesta-
tional age (SGA, AGA, and LGA, respectively), it is
possible to delineate whether the intrauterine growth
was affected in early or late gestation on the basis of
birth weight and corresponding changes in head cir-
cumference and length.7,8 An infant who is propor-
tionately affected in all of its growth parameters is
more likely to have incurred an insult early in ges-
tation, whereas the infant who is disproportionately
affected, with sparing of changes in its length and
head circumference, is more likely to have incurred
an insult later in pregnancy.7,8
We hypothesized that hypospadias is associated
with restriction in fetal growth early in pregnancy.
The aim of this study was to describe the association
between hypospadias and intrauterine growth and
to characterize some of the risk factors that may be
involved.
METHODS
We undertook a retrospective cohort study of a total of 6746
male admissions to the neonatal intensive care units (NICUs) at
University of Connecticut Health Center (UCHC; 3157) and the
Connecticut Children’s Medical Center (3589) between January
1987 and April 2000. These NICUs take care of infants from the
central and northern regions of the state of Connecticut. It is
important to note that the reason for NICU admission was not
PEDIATRICS Vol. 109 No. 3 March 2002 473
hypospadias in any of these infants. Diagnosis was based on
physical examination by an essentially constant group of caregiv-
ers over the entire period of the study. Confirmation of the diag-
nosis and grading of severity were done by consultant urologists.
In cases in which ambiguous genitalia were noted, other causes,
such as congenital adrenal hyperplasia, were ruled out by labora-
tory testing before male gender was ascribed to the infant.9
Eight infants with multiple anomalies or recognized genetic
syndromes associated with hypospadias were excluded from the
analysis. Presence of cryptorchidism was not used as a criterion
for exclusion. Additional analyses were done on the remaining
6738 infants.
Data were collected prospectively on all admissions, based on a
standard questionnaire or data sheet, and entered into computer-
ized databases. From these fields of data sets, queries for infant
data included information on gestational age (GA), birth weight
(BW), birth length, occipitofrontal head circumference at birth,
and singleton versus multiple births. Queries for maternal data
included information on maternal age, race, gravidity, use of
alcohol, use of tobacco, substance abuse, maternal diabetes, hy-
pertension, or preeclampsia. For the purpose of this analysis,
infants were classified on weight percentiles based on the Con-
necticut standards published in 1996.10 Infants less than the 10th
percentile were considered SGA, whereas those greater than 90th
percentile were LGA; the rest were considered AGA. For head
circumference and length, current growth percentile standards by
Britton et al,11 compiled from a large US population, were used.
Statistical analyses of data included regression analyses of hy-
pospadias frequency in relation to the growth parameters studied.
Univariate analyses of risk factors were done using t test for
continuous variables and ␹2 test for nominal variables. Signifi-
cance was determined by P ⬍ .05. The statistical package StatView
4.0 (Abacus Concepts, Inc, Sunnyvale, CA) was used for these
analyses.
RESULTS
Temporal Trends: Increasing Frequency of Occurrence
of Hypospadias
Figure 1 shows the frequency of occurrence of
hypospadias in the 2 NICUs for the 13 years of study
from 1987 to 2000. Hypospadias frequency in both
NICUs increased from 0.4% in 1987 to 4% in the first
quarter of 2000. This was a significant increase with
time (P ⫽ .025), and the relationship of hypospadias
frequency to time was linear (R2 ⫽ 0.921) by a re-
gression analysis. The referral patterns and popula-
tion distribution (GA, BW, proportion of SGA in-
fants, race, gender, and mortality rate) of infants did
Fig 1. Temporal trends in the frequency of occurrence of hypos-
padias in 2 NICUs in Connecticut (N ⫽ 6738 male infants). Data
from January 1987 to April 2000. Significant trends in increase in
frequency shown by linear regression analysis.
474 HYPOSPADIAS AND INTRAUTERINE GROWTH
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not change significantly during the period of study
(data not shown).
Hypospadias and Prematurity
As this study was done in a NICU population, we
investigated whether the change in frequency was
related to prematurity. Figure 2 shows that there was
no relationship of the frequency of hypospadias to
the gestational age (at birth) of an infant. The fre-
quency of hypospadias ranged between 0% and 3.5%
across the 23- to 43-week range of gestational ages.
Hypospadias Is Associated With Poor Intrauterine
Growth
Intrauterine growth was assessed with 3 parame-
ters: BW, length, and head circumference. Figure 3
shows the relationship of hypospadias to intrauter-
ine growth. Of the total number (6738 infants) of
male admissions, 1122 were SGA. Hypospadias was
found in 43 (3.83%) of the 1122 SGA infants com-
pared with 59 (1.27%) of the 4626 AGA infants and 2
of the 505 LGA infants (0.39%; P ⬍ .0001). A total of
487 infants did not have complete growth data for
evaluation.
Hypospadias and BW Percentiles
To eliminate the impact of GA on BW, we corre-
lated the percentiles of BW for each GA category
with the occurrence of hypospadias. As shown in Fig
4, when infants were stratified by the BW percen-
tile,10 we found that there was a significant associa-
tion (P ⬍ .0001) of increased frequency of hypospa-
dias in the lowest weight (3rd–10th) centiles and
conversely the least frequency in the highest weight
(90th–97th) centiles. There was a logarithmic rela-
tionship as shown by the best-fit curve, with R2 ⫽
0.98.
Hypospadias and Birth Length Percentiles
Change in longitudinal growth follows changes in
weight when there are chronic problems of fetal
growth. When hypospadias frequency was plotted
(Fig 5) with birth length percentiles,11 we found that
there was a significant association (P ⬍ .02) of in-
creased frequency of hypospadias in the lowest
length (3rd–25th) centiles and, conversely, the least
Fig 2. Lack of any relationship of frequency of hypospadias to
gestational age at birth in the study population (N ⫽ 6738).

Fig 3. Flow diagram showing the distribution of infants with
hypospadias in relation to their intrauterine growth status. Statis-
tical analysis by the ␹2 test.
Fig 4. Relationship of hypospadias frequency to percentiles of
BW in newborn infants (based on local Connecticut norms10).
There was a significant inverse relationship.
Fig 5. Relationship of hypospadias frequency to percentiles of
birth length in newborn infants (based on current US norms11).
There was a significant inverse relationship.
frequency within the highest length (75th–97th) cen-
tiles. There was a linear relationship with R2 ⫽ 0.93.
Hypospadias and Head Circumference Percentiles
In problems of fetal growth, head circumference is
the last to be affected among the 3 parameters stud-
ied. In Fig 6, hypospadias frequency was plotted
with head circumference percentiles,11 and, again,
there was a significant association (P ⬍ .0004) of
increased frequency of hypospadias in the lowest
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Fig 6. Relationship of hypospadias frequency to percentiles of
head circumference at birth in newborn infants (based on current
US norms11). There was a significant inverse relationship.
head circumference (3rd–25th) centiles and, con-
versely, the least frequency within the highest head
circumference (75th–97th) centiles. There was a lin-
ear relationship as shown by the best-fit curve with
R2 ⫽ 0.98.
Combination of Growth Parameters and Hypospadias
Frequency
To add support to our hypothesis, we found that
when infant growth was affected in all 3 parameters,
(weight, length, and head circumference), the maxi-
mum occurrence of hypospadias (4.5%) was found.
In contrast, when none of these growth parameters
were ⬍10th percentile, the frequency of hypospadias
was markedly lower (1.1%) and this difference was
statistically significant (P ⬍ .05).
Maternal Factors and Hypospadias
We investigated whether any of the maternal fac-
tors that are known to affect fetal growth had an
impact on the occurrence of hypospadias. Infants
with hypospadias were born to mothers of a more
advanced age (29.0 ⫾ 0.6 years; mean ⫾ standard
error of the mean [SEM]) compared with mothers of
nonaffected infants (27.6 ⫾ 0.8 years; mean ⫾ SEM),
but the difference did not reach statistical signifi-
cance (P ⫽ .057). However, the frequency of occur-
rence of hypospadias was higher among first-born
infants (1.9%) compared with all other infants (0.9%),
and this difference was statistically significant (P ⬍
.001). There were no differences in the occurrence of
hypospadias associated with differences in maternal
race or diagnosis of gestational diabetes, hyperten-
sion, or preeclampsia. There were also no significant
differences between singletons and multiple births
(data not shown). The information about the mater-
nal use of alcohol, tobacco, or illicit drugs was avail-
able only from 1 center (UCHC). None of these 3
factors was significantly associated with the inci-
dence of hypospadias (data not shown).
Severity of Hypospadias
The severity of hypospadias, based on meatal
opening, was available from all 52 infants from 1
of the sites (UCHC). An anterior hypospadias (glan-
dular and distal penile) was seen in 65%, mid-hypo-
spadias was seen in 12%, and posterior hypospadias
ARTICLES 475
was seen in 23% of the infants. The BW percentile of
infants with anterior hypospadias was 24 ⫾ 4 centile
(mean ⫾ SEM) and those with posterior hypospadias
was 13 ⫾ 7 centile (mean ⫾ SEM); however, because
of relatively small numbers, a relationship of severity
of hypospadias to severity of growth restriction
could not be made definitively. Of note were 4 in-
fants with severe symmetric growth retardation
whose hypospadias was so severe that gender as-
signment initially was not possible at birth. Only
after investigations for ambiguous genitalia were
completed was it confirmed that these were male
infants with severe hypospadias. Cryptorchidism
was present as a related diagnosis with hypospadias
in 27% of infants.
DISCUSSION
We found a trend of increasing incidence of hypo-
spadias between January 1987 and April 2000 in a
selected population of infants who were admitted to
tertiary-level NICUs in Connecticut. This 10-fold in-
crease was not explained by changes in referral pat-
terns or criteria for diagnosis in these centers and
probably reflects a true overall increase in incidence.
These data agree with the trends suggested by Pau-
lozzi et al3 and, more recently, by Riley et al,12 but
the magnitude of change is much greater. It is ex-
tremely difficult to obtain retrospective population-
based data on the true state or nationwide incidence
of hypospadias because congenital defect registries
do not usually track this malformation. We believe
that the current report reinforces the need for pro-
spective tracking of the incidence of hypospadias at
the state and national levels.
A significant association between hypospadias
and intrauterine growth restriction is demonstrated
in this report. This association with low BW has been
suggested6,13,14 but has not previously been well
characterized. A recent report reaffirmed the associ-
ation and speculated on factors involved in critical
periods early in gestation.15 It is widely known that
factors that inhibit intrauterine growth of the infant
cause a proportionate decrease in somatic (weight
and length) and brain (head circumference) growth if
these factors are active early in gestation.7,8 If growth
inhibition is caused by nutritional and other factors
in the second and third trimesters of gestation, then
there is relative sparing of brain growth (head cir-
cumference) with a predominant effect on weight
initially and changes in length occurring after a more
prolonged insult.7,8 In our analyses of intrauterine
growth parameters, we found that there was a con-
sistent involvement of growth parameters, ie,
weight, length, and head circumference, in infants
with hypospadias, and this finding is highly sugges-
tive of an effect in early gestation on growth—per-
haps early enough to affect adversely urethral devel-
opment.
There was a higher incidence (35%) of the more
severe forms of hypospadias, ie, mid- and posterior,
in our study. This represents a notable increase in the
severity of hypospadias compared with a previous
study, which reported that only approximately 13%
of the cases were of the severe type.16 The reason for
476 HYPOSPADIAS AND INTRAUTERINE GROWTH
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this is not clear, but with the increase in incidence of
hypospadias that has been noted, the increase in
severity is not unexpected and strengthens the argu-
ment that external factors may be involved.
The causes for poor intrauterine growth are many.
A myriad of factors, from genetic to placental to
maternal conditions and environmental changes,
have been studied. Some of these factors, such as
maternal preeclampsia,17 gestational diabetes, and
the use of alcohol or tobacco or substance abuse by
the mother,4,18 were investigated in our study and
were not found to be important in relation to the
occurrence of hypospadias in the infant. A higher
incidence of hypospadias was seen in first-born in-
fants in our study. Although this has been noted in a
previous report,19 no explanation has been forthcom-
ing. It is known that first-born infants are more likely
to have growth restriction,20 and the relationship to
hypospadias may be linked to this observation. Ad-
vanced maternal age was found to be a risk factor by
Fisch et al.21 In our study, there was a similar trend,
but no significant relationship could be demon-
strated.
There is controversy regarding the pathogenesis of
hypospadias.1,22,23 Heinonen et al24 and Kallen and
colleagues4,13 explored various maternal risk factors,
but no definite conclusions were drawn. Many are of
the opinion that it is unlikely that genetic factors play
a role.25–28 Recent evidence from animal29,30 and hu-
man studies31,32 has suggested a relationship of male
genital abnormalities with environmental factors.
Hypospadias is more commonly seen in infants of
infertile couples who conceived by in vitro fertiliza-
tion33 or other assisted reproductive techniques.34
Fertility drugs have been suspected, but a recent
meta-analysis failed to show an effect.35 Anti-andro-
gen compounds have been implicated36; especially
the environmental anti-androgens or “endocrine dis-
rupter chemicals” (EDCs) have been implicated in
causing male genital abnormalities, including hypo-
spadias in humans,37,38 and there seems to be in-
creasing evidence for this from animal studies.39 – 41
Conversely, in case reports of overdosage with me-
droxyprogesterone in early pregnancy, there seemed
to be no adverse effects on the fetus.42 However, it is
important to bear in mind that the exposure of the
EDCs, albeit in low concentrations, is continual and
starts from conception through the most critical pe-
riods of early embryogenesis. Moreover, the pres-
ence of a combination of these agents may have more
than an additive effect.
Because a cause-effect relationship has not been
demonstrated between known factors for poor
growth and hypospadias, we speculate involvement
of other unknown factors. A common theme that
may perhaps interrelate the incidence of growth re-
striction and hypospadias is the report that some
environmental factors, especially “endocrine dis-
rupters,” have been shown to be associated with
both growth restriction and hypospadias. Heindel
et al39 showed that contaminated groundwater can
cause both growth restriction and reproductive
abnormalities in mice. Gray et al40,41 demonstrated
similar effects with some well-known EDCs with
anti-androgenic activity, including linuron and poly-
chlorinated biphenyls congener 169. In a case-control
study, maternal testosterone levels at 6 to 14 weeks’
gestation were significantly lower in pregnancies
that resulted in growth restriction and male genital
abnormalities.43 Because a major part of testosterone
in this period is derived from the placental-fetal unit,
it would not be unreasonable to implicate this unit
and the effects of anti-androgens on it in the patho-
genesis of hypospadias. The alteration of the fetal-
placental-maternal unit’s interaction by any factors
perhaps also disturbs early pregnancy growth and
increases the risk for hypospadias.13
Of additional concern may be that the cumulative
toxicities of EDCs, especially polychlorinated biphe-
nyls, which have an anti-androgen effect, have al-
ready been demonstrated in the aquatic life around
Connecticut, from where this study is reported.44,45
The relationship of these chemicals to human effects
is still unknown. However, the alarming increase in
hypospadias frequency and severity in this region
with no reasonable explanation needs a detailed sci-
entific investigation. This point was well emphasized
by Dolk,46 who after a review of current literature on
the incidence of hypospadias commented, “There is
enough evidence to take the apparent increase in
prevalence of hypospadias seriously,” and suggests
that “efforts to investigate the etiology of hypospa-
dias, including assessment of the endocrine-dis-
rupter hypothesis, must be renewed.”
A major limitation of this study is that it is based
on data from a NICU population of infants. How-
ever, none of the infants was admitted with the pri-
mary diagnosis of hypospadias, and because there is
adequate representation of infants of all gestational
ages, we believe that the data presented here are still
clinically relevant. Because this study was retrospec-
tive, our findings would be more useful in the for-
mulation of a hypothesis rather than in providing
answers; therefore, more population-based prospec-
tive studies need to be performed before any final
conclusions can be drawn.
ACKNOWLEDGMENTS
We thank Susan Zaremba, Marlene Holman, Marta Baker, and
Barbara Westman for invaluable help with data collection and
retrieval.
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IMPACT OF RESEARCH

“The main aim of health research is to improve the health of people. Yet the
performance of researchers tends to be measured by the scientific quality of their
research rather than by its impact on health. This is unsatisfactory, even nonsen-
sical, so a committee on the Royal Netherlands Academy of Arts and Sciences is
trying to devise a way of measuring the social impact of applied health research.
Its first report was discussed at a recent meeting in Amsterdam,1 and the Academy
now plans to experiment with methods of measuring social impact.”

REFERENCE
1. Health Sciences Subcommittee of the Medical Committee of the Royal Netherlands Academy of Arts and Sciences.
The Societal Impact of Applied Health Research: Towards a Quality Assessment System. Amsterdam, the Netherlands:
KNAW; 2001. www.knaw.nl/cg

Smith R. Measuring the social impact of research. BMJ. 2001;323:528

Submitted by Student

478 HYPOSPADIAS AND INTRAUTERINE GROWTH

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 Hypospadias and Early Gestation Growth Restriction in Infants
Naveed Hussain, Azhar Chaghtai, C. D. Anthony Herndon, Victor C. Herson, Ted S.
Rosenkrantz and Patrick H. McKenna
Pediatrics 2002;109;473-478
DOI: 10.1542/peds.109.3.473
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