CONFIDENTIAL ASIJUN 2019/B10462 UNIVERSITI TEKNOLOGI MARA FINAL EXAMINATION COURSE : BIOCHEMISTRY COURSE CODE : Blo462 EXAMINATION : JUNE 2019 TIME : 2HOURS INSTRUCTIONS TO CANDIDATES 1 This question paper consists of five (5) questions. 2. Answer ALL questions in the Answer Booklet. Start each answer on a new page. 3. Do not bring any material into the examination room unless permission is given by the invigilator. 4 Please check to make sure that this examination pack consists of: i) the Question Paper ji) an Answer Booklet — provided by the Faculty 5. Answer ALL questions in English. DO NOT TURN THIS PAGE UNTIL YOU ARE TOLD TO DO SO This examination paper consists of 4 printed page (© Hak Cipta Universiti Teknologi MARA CONFIDENTIALCONFIDENTIAL, 2 ASIJUN 2019/B10462 QUESTION 14 8) State the First and Second Laws of Thermodynamics. (2 marks) b) Determine why hydrocarbon compounds form separate layer when mixed with water. (2 marks) c) i) Find the pK. of benzoic acid with Ka = 6.5 x 10%. (2 marks) ii) Caloulate the pH if you add 3 mL of 0.1 M HCI to 97 mL of water at pH 7.0. (3 marks) QUESTION 2 8) There are 20 types of amino acids involved in protein synthesis in living organisms, 1) Define chirality of amino acid. (1 mark) ji) State one example of chiral and achiral amino acids. (2 marks) iii) Mlustrate and label the peptide bond which forms when two amino acids condensed together and name the process that forms the bond. (4marks) b) State an example of a coenzyme. (1. mark) c) Explain the induced fit model in enzymatic reactions. (3marks) d) Given are two Km values: 4.5 mM and 10 mM. Elucidate which Km produces higher affinity of substrate to enzyme. (2 marks) ©) The urea cycle excretes excess nitrogen from animals back to the environment. Give the name of a compound that is linked to the TCA cycle, (1 mark) ) By using the chemical structure of zwitterions, explain the reaction on the zwitterions if the pH of the solution is increased. (5 marks) (© Hak Cipta Universiti Teknologi MARA, CONFIDENTIAL,CONFIDENTIAL 3 ASIJUN 2019/810462 g) Compare between ion exchange chromatography and affinity chromatography. (4marks) QUESTION 3 8) State the monomer of carbohydrates. (1 mark) b) Give an example of a disaccharide. (1 mark) ©) Describe the fate of pyruvate during vigorous exercise. (2 marks) @) Glucose has a chemical formula of CxH120s. Illustrate the Haworth projection of #-D- Glucose, (2marks) ) Distinguish between amylose and glycogen. (4 marks) ) Mlustrate the TCA cycle. (10 marks) QUESTION 4 a) lustrate the triacylglycerol structure. (1 mark) b) i) State the classes of lipids. (S marks) i) Briefly describe unsaturated fatty acids. (8 marks) c) Explain the consequences for a person who undergoes Atkin’s diet (high in lipid, low in a : (3 marks) d) Biological membranes possess the fluid properties which depend on the composition of the bilayer. i) Mustrate a simple diagram to represent a Upid biayer membrane, Label the hydrophobic and ooo ‘the membrane. A ; (© Hak Cipta Universiti Teknolog! MARA CONFIDENTIALCONFIDENTIAL 4 ASIJUN 2019/B10462 ji) Compare the functional groups between the hydrophilic and hydrophobic portions of the membrane, (2 marks) QUESTION 5 a) i) Define codon and anticodon. (2 marks) li) State one of the stop codons. (1 mark) b) Find the complete base composition of a double-stranded eukaryotic DNA that contains 28% adenine, (3 marks) ©) Describe the steps in RNA translation. (6 marks) END OF QUESTION PAPER (© Hak Cipta Universiti Teknologi MARA, CONFIDENTIAL,