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SCION™ GC Series
The Gas Chromatographers Choice for Separations
GAS CHROMATOGRAPHY
Innovation with Integrity
Innovation in Gas Chromatography
Bruker’s long tradition of innovation and product reliability have combined to create the
next generation of Bruker Gas Chromatographs. By understanding and then designing
to exceed the most critical performance and reliability needs of GC users, Bruker is
delivering systems that are especially for, and all about, the ultimate success of the GC
user. The new SCION 436-GC and SCION 456-GC have have been designed to meet
the most important user specified requirements – reliable performance, ease of use
and simple maintenance.
Local User Interface Fast, Flexible Detection

This large, high resolution display makes Bruker’s comprehensive range of detec-
all GC functions accessible via touch tors deliver industry leading sensitivity,
screen control and “instant access” ease of operation and outstanding
buttons. Easy to navigate and adapt it reliability. And, now all Bruker detectors
comes available in 13 languages for ease feature fast sampling data rate (600Hz)
of local training and support. for rapid separations and greater analysis
throughput.
Multi-language capability
SCION 436-GC
Choice of multi inlet systems

• 2 injectors from 5 available
• Gas Sampling Valve
• Liquid Sampling Valve
Choice of 7 GC
traditional detectors Choice of MS detector
• Universal • Single Quadrupole (MS)
• Specific • Triple Quadrupole (MS/MS)
SCION 456-GC
Choice of multi inlet systems

• 3 injectors from 5 available
• Gas Sampling Valve
• Liquid Sampling Valve
Choice of 7 GC Choice of MS detector

traditional detectors
• Single Quadrupole (MS)
• Universal • Triple Quadrupole (MS/MS)
• Specific
Enhanced Operator Benefits
Bruker offers a range SCION GCs to meet virtually all application

requirements. All SCION GCs are equipped with the convenience of
advanced EFC. Whatever the requirement, we have the solution.
GC Control From Anywhere Turnkey Analyzer Solutions

The unique embedded control Bruker configures and tests GC hardware
architecture incorporated into the GC and software according to widely used
enables the use of remote user interface industry standard methods (e.g. ASTM,
software. This offers the user the ability UOP, EN, ISO, GPA), to save its clients
to control the GC in the exact same way time and to ensure confidence in results.
and with the same level of functionality Standard analyzers are configured to
as if they were standing at the GC using meet the performance specifications
the User Interface but from a remote outlined in the method itself. Included in
location, even from home. these analyzer packages:
• All hardware
Gas Saving Capability • Software (including special applica-
Essential to any laboratory is the tion “plug-ins” when appropriate)
requirement for cost control. Gas • Pre-installed methods
consumption is a major source of • Test chromatograms
operational cost and reduction in gas • Installation/validation data
flow when not necessary is a vital • User documentation customized to
function of any modern GC. Bruker GCs the specific method
have the necessary functionality to save
valuable gas and thus costs.
LAN
Targeted Solutions for Simulated Distallation
Specific Markets
A series of software customization
tools allow users to develop unique
calculation modules, that fully integrate
with CompassCDS. A large number of
standard plug-ins are available that allow
special reporting and other post analysis
functions. Some examples include:
Simulated Distillation
Provides automated boiling point
distributions for a full range of petroleum
products for applications that comply
with ASTM, IP, DIN and ISO standard
test methods.
Detailed Hydrocarbon Analysis
Reports in an automated way the CompassCDS PeakSynch
physical properties of gasoline and
similar products based on individual
components for applications that comply
with Bruker developed methods and
ASTM, IP and standard test methods.
CompassCDS PeakSynch
Provides rapid visual and quantitative
comparison of complex chromatograms
and is widely used in the flavor and
fragrance industry.
436 GC
Small dedicated high trough putt
system
Benefits of the
SCION-GC
n Multi-language User Interface
n Full EFC Capability
n High Pressure Injection
n IntelliUpdate
n System Suitability Determination
n CompassCDS Software
n 600 Hz Data Sampling Rate On All Detectors
n Inert GC Sample Path
n Constant Linear Velocity Mode
n Fast Cycle Time
SCION 436-GC
n Small foot print
n High performance
n Dual channel architecture
SCION 456-GC
n Solutions platform
n Total flexibility
n Four channel
architecture
Increased Productivity
With over 40 years’ experience in gas chromatography, we can provide
unrivalled expertise, not only in building robust instruments, but also
in creating solutions for ensuring productivity. W ith tot al control over
design and manufacturing , Bruker ensures the quality and technological
excellence of its products is complimented and combined with features
that deliver the true benefits of productivity.
Speed increased with a factor 6.5

n Small ID from 0.25 to 0.1mm
n Short column from 15 to 4 mtr
n Increased ramp from 10 to 65 ºC/min
n Data rate from 25 to 20 0 Hz
Fast Cycle Time

The time between injections can
considerably improve productivity. The
high performance oven incorporating minutes
design characteristics that enhances fast
heating and cooling ensures maximum
productivity. This, in conjunction with
high pressure injectors and ultra narrow
bore columns will significantly improve
and yield fast cycle times without loss of
performance (see chromatograms).
minutes
IntelliUpdate
In many cases instrument and system
effects (column ageing, matrix, etc.) can
cause experimental deviations e.g.
retention time. CompassCDS
IntelliUpdate function can be used auto-
matically to correct and compensate for
such deviations. This unique capability is
also done without changing fundamental
instrument parameters, maintaining accu-
racy of results and method continuity.
Optimized Switching Valves Comparison analysis with and
Flow splitting, backflushing and Deans without backflush
switching are valuable techniques in

improving cycle times, analytical per-
formance and the robustness of GC
methods. Splitting the flow of column
effluent into differing detectors can
enhance performance, quantitation and
confirmation of targeted compounds.
Backflushing is key to reducing analysis
time and column protection. It works on
the basis of reversing column flow after
peaks of interest have been detected.
This eliminates the need for time and
temperature segments to elute highly
retained components injected with com-
pounds of interest. Reversing the flow
elutes these materials out through the
split vent of the injector with the added
benefit of protecting the column from
degradation and contamination.
Backflushing capability also allows
column changes and injector mainte-
nance without loss of vacuum in the MS
detector.
Heart cut using the Deans switch
Deans switching enables the use of

multiple traps and columns of differing
phases in one method/analysis. It is the
basis of two dimensional GC and many
standard analyzers manufactured by
Bruker. Use our expertize to configure
the optimum system for you.
CompassCDS software
Capability and Automation
Bruker offers an injector and detector range to meet virtually all application and
market requirements. All are equipped with the convenience of advanced EFC.
Whatever the requirement, be it Split/splitless, Cold-on-Column, Packed, Flash or Pro-
grammable Temperature Vapourizing injector with a universal or specific detector we
have the solution.
Universal Specific
FID TCD PHHID MS ECD NPD (TSD) PFPD
✓ ✓ ✓ ✓
ca
A
d e mi
c
nv ✓ ✓ ✓ ✓ ✓
nt
E
ir o m e
Food
✓ ✓ ✓ ✓ ✓ ✓
B
ev
e
erag
rensic
Fo
✓ ✓ ✓
s
ox
y
T
ic o l o g
et
✓ ✓ ✓ ✓ ✓ ✓ ✓
P
ro le u
Bruker offers a range of differing injector

designs for all applications, column dimensi-
ons and can be fully automated.
Injector Selection Guide - Sample/Analysis Characteristics or Requirements

Trace Separation Sample Wide Range Preferred Column Type 1st Choice 2nd Choice
Analysis & Speed Capacity of Analytes
✓ Capillary, 0.53 mm ID Large Volume (LV) Split/Splitless
✓ Capillary, 0.1 to 0.53 mm ID Split/Splitless Large Volume (LV) SS Mode
✓ ✓ Capillary, 0.53 mm ID Large Volume (LV)
✓ Capillary, retention gap Cold On-Column Large Volume PTV Mode
✓ Capillary, 0.53 mm ID Packed Large Volume (LV) PTV Mode
✓ Capillary, 0.53 mm ID Cold On-Column Large Volume (LV) On-Column Mode

Regardless of your sample throughput requirements, Bruker can provide
an automated solution to meet your needs. Four samplers are available,
the CP-8410, CP-8400, the SHS-40 and the PAL Combi-xt. Each is
tailored to meet a differing need and workload.
CP-840 0
n High throughput
n 100 x 2ml sample capacity
n Dual/Duplicate Injection
n SPME
CP-8410
n Flexibility
n Accommodates 2, 5, 10 ml vials
n Low cost/high performance
n Ease of use
SHS-40
n Fully automated
n Easily Integrated
n Low maintenance
n Sample Loop or Press and Inject configuration
PAL Combi-xt
n High throughput
n Liquid handling capability
n SPME
n ITEX
Bruker-Certified Consumables for
Your SCION GC Series
Bruker GC columns span a broad range of column diameters,
stationary phases, and capillary column materials: Fused Silica
(FS) and Inert Steel (IS). Ideal for either routine or research type
analyses.
Bruker GC column offerings bridge across many important
applications and include a number of offerings such as:
Standard WCOT (Wall Coated Open Tubular)
Solid Stationary Phase PLOT (Porous Layer Open Tubular)
Inert Steel Micro-Packed and Packed
Super Clean™ Gas Filters

Bruker Gas Purification Systems have the range to satisfy
your needs from individual to combination filters, from Ultra
purity combined with Ultra capacity, to all in one solution
kits. Innovative features designed into the product yield
extensive benefits to the user.
Bruker Daltonics is continually improving its products and reserves the right to change specifications without notice.
Ultra-high capacity for long life, less change and improved
productivity
High-purity output ensures 99.9999% Pure Gas
“Quick connect“ fittings for easy, leak-tight filter changes
© BDAL 04-2012. #281754 Super Clean™ is a registered trademark of SGT Middelburg B.V.
Glass internals prevent diffusion; plastic externally for safety
Easy-to-read indicators for planned maintenance and
improved up-time
For research use only. Not for use in diagnostic procedures.
www.bruker.com/scion
SCION 436-GC
Specification Sheet
Dimensions and Weights

Size*: Height: 57 cm (20.9 in.),
Width: 32 cm (26.0 in.),
Depth: 61 cm (22.0 in.)
Weight*: 26.8 kg (59 lb)
* Typical values
Environmental Conditions
Operating temperatures: 10 °C to 40 °C.
Operating humidity (relative): 5 % to 95 %
Line voltage requirements:, 120 V, 230 V (±10 % nominal)
Column Oven
Temperature Range (°C) 436-GC Rates (°C/min)
Dimensions: 23 cm (w) x 11 cm (d) x 28 cm (h)
Temperature range: 50 -70 170
Ambient +4 °C to 450 °C
70 - 115 105
Liquid N2: -100 °C to 450 °C
Liquid CO2: -60 °C to 450 °C 115 - 175 80
175 - 300 55
Temperature program ramps/holds: 24/25
Maximum temperature ramp rate: 170°C/min for all voltages 300 - 450 35
Cool down rate: 400 °C to 50 °C in 4.5 minutes

Temperature set-point resolution: 0.1 °C
Ambient temperature reject <0.01°C change in oven for 1°C change in ambient temp
Retention Time Repeatability <0.008% or < 0.0008 min, based on Pentadecane under temperature program
conditions
Area repeatability < 1% RSD
General Specifications
Up to 3 EFC modules total, injector, detector and auxiliary
Optional backflush
GC Control:
External events (digital output):
• 8 standard
• 8 optional, total 16
Max number of timed events: 30
Heated zones:
• Standard 5
Two power outlets 24V (1A max. each)

Bruker Daltonics is continually improving its products and
reserves the right to change specifications without notice.
© Bruker Daltonics 04-2012, #CA-701221
www.bruker.com/chemicalanalysis
Methods:
Maximum stored internal methods: 50 (max. 30 alphanumeric characters)
Logging:
Run log file (stored with the chromatogram when using CompassCDS)
Error log file
Local Display:
TFT full color screen
WVGA resolution (800 x 480)
Size 23 cm (9")
Local Control:
Touch screen
Hard keys
Languages:
English, German, French, Spanish, Italian, Portuguese, Cyrillic, Kanji, Chinese (standard and traditional),
Thai, Korean and Dutch.
Local automation:
Method lines: 25
Modes:
• Infinite looping
• Dual and duplicate injection
Communication
Ethernet: Protocol: TCP/IP
Data rate: 100 Mbps
Control: GC control and method parameters
Analog output (optional):
Number of channels: 1
Time programmable steps: 30
Output software selectable (set individual):
• 0-1 V (default)
• 0-10 V
Synchronization signals with other devices and data systems:

Ready in and out
Start in and out
Data Handling and System Control:

CompassCDS Chromatography Data System
Certifications
CSA:
• C22.2 61010-1
• UL 61010-1
IEC: 61010-1
EMC:
• 47 CFR part 15
• ANSI C63.4
• EN 61326

Injector Options
Maximum injectors: two, operating concurrently
Pneumatics: Electronic Flow Control (EFC), or manual
Injector types:
S/SL Split/Splitless injector*
PTV Programmable Temperature Vaporizing*
COC Cold On-Column injector*
Flash injector
PWOC Packed/Wide bore On-Column injector
*Including septum purge
S/SL Split/Splitless Injector

Pressure range: 0-150 psi
Total flow:
500 mL/min for N2 /Ar
1500 mL/min for He/H2
Maximum temperature: 450 °C
Split range: 1-10,000 (column dependent)
Suited for columns:

Wide bore: (0.53 mm)
Narrow bore: (0.05 to 0.32 mm)
COC Cold On-Column Injector

Total Flow: 50 mL/min (Type 23 EFC)
Temperature range:
Ambient +10 °C to 450 °C using air cooling
-60 °C to 450 °C using liquid CO2 cooling
-160 °C to 450 °C using liquid N2 cooling

Maximum temperature ramp rate: 200 °C/min
Temperature ramps/holds: 24/25
Suited for columns:

Wide bore (0.53 mm)
Narrow bore (0.32 mm)
PTV Programmable Temperature

Vaporizing Injector
Total flow:
1500 ml/min for He/H2
Temperature range:
Ambient + 10 °C to 450 °C using air cooling


Operational capabilities:
Large volume injection
Temperature ramped splitless
Cold on-column
Split and splitless
ChromatoProbe solid sample introduction optional
Suited for columns:

Wide bore (0.53 mm)
Narrow bore (0.05 to 0.32 mm)
Maximum injection volume: 250 μL (LVI mode)
Flash Injector
Total flow:
50 mL/min (Type 23 EFC)

Suited for columns:
Wide bore (0.53 mm)
Packed (1/8” to 1/4”)
PWOC Packed/Wide-bore On-Column Injector

Total flow:
Suited for columns:

Wide bore (0.53 mm)
Packed (1/8” to 1/4”)
Electronic Flow Control: Injectors (EFC)

Module types: 2 injector-specific modules
Pressure: 0.1 % Full Scale
Resolution pressure set points is 0.001psi
Flow sensor accuracy 2% of measured or 0.2% of full scale
Flow sensor repeatability 0.5%

Quick-Switch Valve Option
Instantly switch between injectors/columns and detectors
Configurations: automated or manual, factory or field installed
Detector Options
Maximum detectors: two: operating concurrently (one of
which is a Single or Triple Quad MS)
Pneumatics: Electronic Flow Control (DEFC) or manual
Detector types:
FID Flame Ionization Detector
TCD Thermal Conductivity Detector
ECD Electron Capture Detector
NPD (TSD) Nitrogen-Phosphorus Detector
PFPD Pulsed Flame Photometric Detector
PDHID Pulsed Discharge Helium Ionization Detector
MS Mass Spectrometry (see GC/MS brochure and datasheet)
Note: Data Acquisition Rate : 600Hz for all detectors, exception is the PFPD

Detectivity: 2 pg C/sec
Linear dynamic range: 107
Flame tip type: ceramic (patented)

Operational quality:
Flame-out detection
Auto re-ignition

Detectivity: 300 pg/mL (Butane)
Linear dynamic range: 10 6
Filament protection
Automatic bridge balancing

Detectivity: 7 fg/s Lindane
Radioactive source: 63Ni - 15 mCi (555 Mbq)

Detectivity:
N: 100 fg N/sec (Azobenzene)
P: 100 fg P/sec (Malathion)
Linear dynamic range:

N: 10 5
P: 10 4
Operational quality: self-aligning bead

Photomultiplier tube:
S/P
S/P/N
Detectivity:
S: 1 pg S/sec (S/P tube)
P: 100 fg P/sec (S/P tube)
N: 20 pg N/sec (S/P/N tube)

S: 10 3
P: 10 4
N: 102
Up to 23 elements can be detected

Detectivity: 50 ppb (Methane)
Linear dynamic range: 10 4 (Methane)
Gold plated connections
Welded column connections
Detectors (DEFC)
Module types: 6 detector-specific modules
Accuracy: ± 7 % set point flow
Resolution: 0.1 or 1 mL/min
Automation Options
CP-8410 Auto Injector

Sample capacity:
10 x 2 mL vials
6 x 5 mL vials
5 x 10 mL vials
Large solvent wash vial: 2 x 120 mL*
Dual and duplicate mode
Internal standard addition
Modes of operation:
Liquid
Ambient headspace*
SPME (Solid Phase MicroExtraction)*
Sample heating and cooling*
Pre-programmed modes of injection Syringes:

1 μL, 2 μL, 5 μL, 10 μL, 100 μL, 250 μL
for liquid injection
SPME
CP-8400 AutoSampler

Sample capacity: 100 x 2 mL vials
Modes of operation:
Liquid
Ambient headspace*
SPME*
Pre-programmed modes of injection
Syringes:
• 1 μL, 2 μL, 5 μL, 10 μL, 100 μL, 250 μL for liquid injection
• SPME
* Optional
PAL Combi-xt AutoSampler

Sample trays: two standard and expandable to four
Tray types:
98 x 2 mL vials
200 x 1 mL vials
32 x 10 mL/20 mL vials
96-well plates
Modes of operation:
Liquid
Heated headspace*
SPME*
ITEX*
Sample heating and cooling
Additional optional modules: further sample trays, micro-well plate holders, wash station,
SPME fiber bake-out station, dilutor, barcode readers, and flowcell
* Optional
SHS-40 Headspace Sampler

System Features
Fixed Sample Volume Configuration
Sample Capacity Up to 40 vials; 20mL

or 10 mL w/adaptor
Crimp or Screw Top Vials
Injection Modes Fixed Sample Volume

Press and Inject
Injection Valve 6 Way Electric Actuated

1 mL Sample Loop
Heat up to 350°C
SHS-40 Incubator Up to 12 Samples

40-200°C in 1°C increments
Integrated Shaker
Instrument Control Remote with Compass CDS

Press and Inject Configuration Stand alone
Sample Recognition Automated

and Detection
Carrier Gas Control Direct from GC
Optional Accessories Additional Sampling Valve

Reactant Gas
Catalytic Converter
SHS-40
Th
Sample Capacity: 40 x 20ml (10ml vials with adaptors).

Fully integrated
Sample path inert
Automated vial queuing system
Automated Gas Sampling Valve- Electric actuated 6-way injection valve (VICI) with 1 ml sample loop. This
entire module may be separately heated up to 350°C.
Heated incubation oven for 12 samples (40°C - 200°C, in 1°C-steps
Integrated Shaker
Automatic sample detection/recognition
Compatible with Crimp and Screw Top Vials
Carrier gas controlled direct from the GC
AUX gas pre-set internally.
Up to 9 different parameter sets can be stored.
Adaptable to different injectors
Multiple Headspace Extraction mode via single puncture
Flexible transfer line
Option: Sample Transfer Line Kit (direct connection to carrier gas, insertion of transfer line via injector no
longer required)
Control: Stand alone or remotely via Compass CDS software
Voltage: 110V or 230V
Dimensions: Width: 29cm (12in) Height: 46cm (18in) Depth: 62cm (25in)
Weight: 21kg (47lb)

SCION 456-GC
Specification Sheet
Dimensions and Weights

Size*: Height: 57 cm (22.5 in.)
Width: 66 cm (26.0 in.)
Depth: 56 cm (22.0 in.)
Weight*: 43 kg (95 lb)
* Typical values
Environmental Conditions
Operating temperatures: 10 °C to 40 °C.
Operating humidity (relative): 5 % to 95 %
Line voltage requirements:, 120 V, 230 V (±10 % nominal)
Column Oven
Dimensions: 28 cm (w) x 20 cm (d) x 28 cm (h)
Temperature range:
Ambient +4 °C to 450 °C Temperature Range (°C) 456-GC Rates (°C/min)
Liquid N2: -100 °C to 450 °C
Liquid CO2: -60 °C to 450 °C 50 -70 150
70 - 115 95
Temperature program ramps/holds: 24/25
115 - 175 70
Maximum temperature ramp rate: 150°C/min for all voltages
and 180 °C/min with oven insert accessory 175 - 300 45
300 - 450 30
Cool down rate: 400 °C to 50 °C in 4.5 minutes
Temperature set-point resolution: 0.1 °C
Ambient temperature reject <0.01°C change in oven for 1°C change in ambient temp
Retention time Repeatability <0.008% or < 0.0008 min, based on Pentadecane under temperature program
conditions
Area repeatability < 1% RSD
General Specifications
Up to 9 EFC modules total, injector, detector and auxiliary
Optional backflush
GC Control:
External events (digital output):
• 8 standard
• 8 optional, total 16
Max number of timed events: 16
Heated zones:
• Standard 5
• 4 optional, 9 total
Two power outlets 24V (1A max. each)
Methods:
Maximum stored internal methods: 50 (max. 30 alphanumeric characters)

Logging:
Run log file (stored with the chromatogram when using CompassCDS)
Error log file
Local Display:
TFT full color screen
WVGA resolution (800 x 480)
Size 23 cm (9")
Local Control:
Touch screen
Hard keys
Languages:
English, German, French, Spanish, Italian, Portuguese, Cyrillic, Kanji, Chinese (standard and traditional),
Thai, Korean and Dutch.
Local automation:
Method lines: 25
Modes:
• Infinite looping
• Dual and duplicate injection
Communication
Ethernet: Protocol: TCP/IP
Data rate: 100 Mbps
Control: GC control and method parameters
Analog output (optional):
Number of channels: 3
Time programmable steps: 30
Output software selectable (set individual):
• 0-1 V (default)
• 0-10 V
Synchronization signals with other devices and data systems:

Ready in and out
Start in and out
Data Handling and System Control:

CompassCDS Chromatography Data System
Certifications
CSA:
• C22.2 61010-1
• UL 61010-1
IEC: 61010-1
EMC:
• 47 CFR part 15
• ANSI C63.4
• EN 61326

Injector Options
Maximum injectors: three, operating concurrently
Pneumatics: Electronic Flow Control (EFC), or manual
Injector types:
S/SL Split/Splitless injector*
PTV Programmable Temperature Vaporizing*
COC Cold On-Column injector*
Flash injector
PWOC Packed/Wide bore On-Column injector
*Including septum purge
S/SL Split/Splitless Injector

Total flow:
1500 mL/min for He/H2
Suited for columns:

Wide bore: (0.53 mm)
Narrow bore: (0.05 to 0.32 mm)
COC Cold On-Column Injector

Total Flow: 50 mL/min (Type 23 EFC)
Temperature range:
Ambient +10 °C to 450 °C using air cooling

Suited for columns:

Wide bore (0.53 mm)
Narrow bore (0.32 mm)
PTV Programmable Temperature

Vaporizing Injector
Total flow:
500 mL/min for N2/Ar
1500 ml/min for He/H2
Temperature range:
Ambient + 10 °C to 450 °C using air cooling


Operational capabilities:
Large volume injection
Temperature ramped splitless
Cold on-column
Split and splitless
ChromatoProbe solid sample introduction optional
Suited for columns:

Wide bore (0.53 mm)
Narrow bore (0.05 to 0.32 mm)
Maximum injection volume: 250 μL (LVI mode)
Flash Injector
Total flow:

Suited for columns:
Wide bore (0.53 mm)
Packed (1/8” to 1/4”)
PWOC Packed/Wide-bore On-Column Injector

Total flow:
Suited for columns:

Wide bore (0.53 mm)
Packed (1/8” to 1/4”)
Electronic Flow Control: Injectors (EFC)

Module types: 4 injector-specific modules
Pressure: 0.1 % Full Scale
Resolution pressure set points is 0.001psi
Flow sensor accuracy 2% of measured or 0.2% of full scale
Flow sensor repeatability 0.5%
Sample Preconcentration Trap (SPT)

Trace level analysis of volatiles in gases
Fully integrated
Temperature range:
Temperature rate:
Ballistic for instant release of adsorbed volatiles
Available traps:
Two lengths
A wide range of standard packings and custom packings

Quick-Switch Valve Option
Instantly switch between injectors/columns and detectors
Configurations: automated or manual, factory or field installed
Detector Options
Maximum detectors: four: operating concurrently (one of
which is a Single or Triple Quad MS)
Pneumatics: Electronic Flow Control (DEFC) or manual
Detector types:
MS Mass Spectrometry (see GC/MS brochure and datasheet)
Note: Data Acquisition Rate : 600Hz for all detectors, exception is the PFPD

Detectivity: 2 pg C/sec
Linear dynamic range: 107
Flame tip type: ceramic (patented)

Flame-out detection
Auto re-ignition

Detectivity: 300 pg/mL (Butane)
Filament protection
Automatic bridge balancing

Detectivity: 7 fg/sec Lindane
Radioactive source: 63Ni - 15 mCi (555 Mbq)

Detectivity:
N: 100 fg N/sec (Azobenzene)
P: 100 fg P/sec (Malathion)

N: 10 5
P: 10 4
Operational quality: self-aligning bead

Photomultiplier tube:
S/P
S/P/N
Detectivity:
S: 1 pg S/sec (S/P tube)
P: 100 fg P/sec (S/P tube)
N: 20 pg N/sec (S/P/N tube)

S: 10 3
P: 10 4
N: 102
Up to 23 elements can be detected

Detectivity: 50 ppb (Methane)
Linear dynamic range: 10 4 (Methane)
Gold plated connections
Welded column connections
Detectors (DEFC)
Module types: 6 detector-specific modules
Accuracy: ± 7 % set point flow
Resolution: 0.1 or 1 mL/min
Automation Options
CP-8410 Auto Injector

Sample capacity:
10 x 2 mL vials
6 x 5 mL vials
5 x 10 mL vials
Modes of operation:
Liquid
Ambient headspace*
SPME (Solid Phase MicroExtraction)*
Pre-programmed modes of injection Syringes:

1 μL, 2 μL, 5 μL, 10 μL, 100 μL, 250 μL for liquid injection
SPME

CP-8400 AutoSampler
Sample capacity: 100 x 2 mL vials
Modes of operation:
Liquid
Ambient headspace*
SPME*
Pre-programmed modes of injection syringes:

1 μL, 2 μL, 5 μL, 10 μL, 100 μL, 250 μL for liquid injection
SPME
* Optional
PAL Combi-xt AutoSampler

Sample trays: two standard and expandable to four
Tray types:
98 x 2 mL vials
200 x 1 mL vials
32 x 10 mL/20 mL vials
96-well plates
Modes of operation:
Liquid
Heated headspace*
SPME*
ITEX*
Sample heating and cooling
Additional optional modules: further sample trays, micro-well plate holders, wash station,
SPME fiber bake-out station, dilutor, barcode readers, and flowcell
* Optional

SHS-40 Headspace Sampler
System Features
Fixed Sample Volume Configuration
Sample Capacity Up to 40 vials; 20mL

or 10 mL w/adaptor
Crimp or Screw Top Vials
Injection Modes Fixed Sample Volume

Press and Inject
Injection Valve 6 Way Electric Actuated

1 mL Sample Loop
Heat up to 350°C
SHS-40 Incubator Up to 12 Samples

40-200°C in 1°C increments
Integrated Shaker
Instrument Control Remote with Compass CDS

Press and Inject Configuration Stand alone
Sample Recognition Automated

and Detection
Carrier Gas Control Direct from GC
Optional Accessories Additional Sampling Valve

Reactant Gas
Catalytic Converter
SHS-40
Th
Sample Capacity: 40 x 20ml (10ml vials with adaptors).

Fully integrated
Sample path inert
Automated vial queuing system
Automated Gas Sampling Valve- Electric actuated 6-way injection valve (VICI) with 1 ml sample loop. This
entire module may be separately heated up to 350°C.
Heated incubation oven for 12 samples (40°C - 200°C, in 1°C-steps
Integrated Shaker
Automatic sample detection/recognition
Compatible with Crimp and Screw Top Vials
Carrier gas controlled direct from the GC
AUX gas pre-set internally.
Up to 9 different parameter sets can be stored.
Adaptable to different injectors
Multiple Headspace Extraction mode via single puncture
Flexible transfer line
Option: Sample Transfer Line Kit (direct connection to carrier gas, insertion of transfer line via injector no
longer required)
Control: Stand alone or remotely via Compass CDS software
Voltage: 110V or 230V
Dimensions: Width: 29cm (12in) Height: 46cm (18in) Depth: 62cm (25in)
Weight: 21kg (47lb)

Refinery
Gas Analyzer
Optimized GC Analysis Solutions
Gas Chromatography
Bruker Refinery Gas Analyzers
The source and composition of refinery gases varies considerably. Measuring gas
composition precisely and accurately is a significant challenge in today’s refinery
operations. Bruker’s refinery gas analyzers are designed to deliver superior, reliable
results for a wide range of sources and analysis throughput requirements.
A range of refinery gas analysis

(RGA) solutions. Bruker offers RGA Operational procedures are fully
solutions to meet the broadest documented. Bruker RGA analyzers
range of stream sample types and not only incorporate proven GC
throughput requirements. hardware and software but also arrive
pre-loaded with analysis methods, and
A powerful, easy to use GC solution. include documentation specific to the
Bruker’s 456-GC and application required.
CompassCDS chromatography
software is a very powerful A comprehensive, single vendor
combination designed to achieve solution. Bruker provides complete
the best possible results. In solutions. The hardware, software,
addition, these systems do not application optimization,
require a high degree of operator skills. documentation, installation and
performance verification are all
A highly flexible solution for analysis. provided by Bruker, offering an all
The Bruker RGA solutions can optional inclusive, convenient analysis solution.
be configured to analyze high
pressurized gas and liquefied petroleum
gas (LPG) through the use of a fully
integrated Micro-Gasifier, giving the
flexibility to accommodate a wide range
of stream types.
Figure 1: The 456-GC RGA has

outstanding flexiblity, analytical
power and robustness.
Key Benefits
Bruker solutions for refinery gas

7 8 9 10
analysis 1. Hydrogen 19. i-Butene
10. Propane
2. Carbon dioxide 11. Cyclo Propane 20. c-2-Butene
Typical sources for refinery gases include 3. Hydrogen sulfide 12. Propylene 21. i-Pentane
atmospheric or FCC overheads, ethylene, 12 4. Oxygen 13. i-Butane 22. n-Pentane
5. Nitrogen 14. n-Butane 23. 1,3-Butadiene
propylene production, fuel gas, stack 6. Carbon monoxide 15. Propadiene 24. Propyne
gas and off gas from desulfurization. The 7. Methane 16. Acetylene 25. Butyne
8. Ethane 17. t-2-Butene 26. C6+
physical stream types range from gas to 9. Ethylene 18. 1-Butene 27. Helium
highly pressurized gas or liquefied gases.
Bruker’s refinery gas analyzers (RGA) 14 19
are ‘turnkey’ systems pre-configured 26 18

21
17 20 24
and tuned at the factory to conform to 13
15
25
11
industry standard methods including: 16 22
UOP 539, DIN-51666 and ASTM

D2163. The RGA systems are based on
Min.
the Bruker 456-GC. To perform good 0 4 8 12 16 20 24 28
analysis, the RGA is optional equipped

with an integrated micro-gasifier. This Figure 2: The separation of light hydrocarbons using the Standard
sample conditioning device ensures RGA.
complete vaporization of LPGs and high
pressures samples to prevent any sample 9 7
discrimination prior injection. 1
The Analyzers employ a proven and 5
optimized multi-channel approach. They

8 27
determine the concentration of individual
saturated and unsaturated hydrocarbon
components up to and including C5 (C6
and higher components as a composite 2
peak) and all permanent gases, including

hydrogen and hydrogen sulfide in a 16
single analysis. Included in every system 3 4 6
is Bruker’s powerful CompassCDS 0 Min. 5

0 Min. 15
chromatography software to provide
complete analyzer control, data
Figure 3: The analysis of the permanent gases and hydrogen (and
acquisition and flexible report generation.
helium) using the Standard RGA.
FCC
Ethylene Propylene Propane Butylene
Overhead
Fuel Gas Stack Gas Off Gas from

Butanes Reformer Gas
Desulphurization
Figure 4: The RGA analyzers are applicable to a variety of different hydrocarbon streams.
Bruker offers two RGA systems to

7 9
meet the widest range of analysis
1. Hydrogen 10. Propane 19. i-Butene
requirements: 10 2. Carbon dioxide 11. Cyclo Propane 20. c-2-Butene
3. Hydrogen sulfide 12. Propylene 21. i-Pentane
Standard RGA: A three channel 8 4. Oxygen 13. i-Butane 22. n-Pentane
12 5. Nitrogen 14. n-Butane 23. 1,3-Butadiene
456-GC with a multi-valve design using 6. Carbon monoxide 15. Propadiene 24. Propyne
both capillary and packed columns. 7. Methane 16. Acetylene 25. Butyne
8. Ethane 17. t-2-Butene 26. C6+
The first channel is optimized for the 9. Ethylene 18. 1-Butene 27. Helium
analysis of permanent gases,
the second is designed for light 14
19
23
24 25
hydrocarbons, and the third specifically 26 11 13 15 16 1718 20 21
21
22
for hydrogen. The system is configured
and fully tested in accordance with 0 1 2 3 4 Min. 5
industry standard methods. Total
analysis time for all components is less Figure 5: The analysis of light hydrocarbons using the Rapid RGA,
than 25 minutes. with complete separation in less than five minutes.
The standard RGA analyzer is the most
powerful tool to analyze the widest
1
range of RGA type streams. This 9 7
includes sample streams with a

high % level of components as in
8
ethylene, propylene and butylene 5
streams.
Rapid RGA: A three channel 456-GC

that utilizes a multi-valve design in 2
which the packed columns used in the

16
Standard RGA are replaced by micro- 3
6
packed columns in both the hydrogen 4
and permanent gas channels. Since 0 Min. 7 0 Min. 2

the micro-packed columns are installed
in a separate heated zone, the capillary Figure 6: The analysis of permanent gases and hydrogen using the
columns located in the GC oven can Rapid RGA.
be temperature programmed in a
more aggressive manner. For high
sample analysis demand, the Rapid
RGA Analyzer concept provides a
substantial reduction in overall analysis
time of 5 minutes ( 7 minutes with
H2S) compared to the 25 minutes with
the standard RGA.
A B
Figure 7: A shows a ‘traditional’ RGA with all columns mounted in

oven. B shows the micro-packed columns mounted in the separate
heated zone in the Rapid RGA.
Table 1: RGA Analyzer Characteristics.
Characteristics Standard RGA Rapid RGA
No. of Channels/Detectors Used 3 3
No. of Column Ovens 1 2
Analysis Time 25 min 5 min (7 min with H2S)
Repeatability <1% <1%
Linear Bench Space Required 66 cm/26 in. 66 cm/26 in.
0.01% all components 0.01% all components

Minimum Component Detection Level
except H2S = 0.05% except H2S = 0.05%
Suitability
Typical Refinery Gas Excellent Excellent
Impurities in Bulk Ethylene Excellent Excellent
Impurities in Bulk Propylene Excellent Good
Impurities in Bulk C4 Good Good
Table 2: Multiple channels of data are conveniently combined into a single analysis report.
Peak Area
Peak Name Channel RT (min.) Result (g/l) Norm. (%)
No. (uV/Sec.)
1 Hydrogen Middle (TCD) 1.6967 36.0300 22.7681 390257
2 Carbon dioxide Front (TCD) 2.6000 0.1000 0.0632 13376
3 Hydrogen sulfide Front (TCD) - 0.0000 0.0000 0
4 Oxygen Front (TCD) 9.9200 0.0000 0.0000 37325
5 Nitrogen Front (TCD) 10.3267 1.1990 0.7577 2122071
6 Carbon monoxide Front (TCD) - 0.0000 0.0000 0
7a Methane Front (TCD) 11.1917 11.9900 7.5767 1394584
7b Methane Rear (FID) 3.7350 11.9900 7.5767 1492388
8a Ethane Front (TCD) 3.5367 17.9900 11.3682 2867688
8b Ethane Rear (FID) 4.1283 17.9900 11.3682 4480322
9a Ethylene Front (TCD) 2.9550 29.9800 18.9449 4139442
9b Ethylene Rear (FID) 4.7217 29.9800 18.9449 7411134
10 Propane Rear (FID) 6.1933 0.1990 0.1258 71402
11 Cyclo Propane Rear (FID) - 0.0000 0.0000 0
12 Propylene Rear (FID) - 0.0000 0.0000 0
13 i-Butane Rear (FID) - 0.0000 0.0000 0
14 n-Butane Rear (FID) - 0.0000 0.0000 0
15 Propadiene Rear (FID) - 0.0000 0.0000 0
16a Acetylene Front (TCD) 5.0283 0.5020 0.3172 49786
16b Acetylene Rear (FID) 16.4331 0.5020 0.3172 121300
17 t-2-Butene Rear (FID) 18.5050 0.0990 0.0626 138647
18 1-Butene Rear (FID) - 0.0000 0.0000 0
19 i-Butene Rear (FID) 19.5167 0.0990 0.0626 44492
20 cis-2-Butene Rear (FID) - 0.0000 0.0000 0
21 1,3-Butadiene Rear (FID) 22.1367 0.0000 0.0000 16165
22 Propyne Rear (FID) - 0.0000 0.0000 0
23 C5+ Rear (FID) 2.9217 0.1000 0.0632 58164
Totals 158.2480 100.0000

analyses.

productivity
improved up-time
Bruker Daltonics is continually improving its products and reserves the right
to change specifications without notice. © BDAL 05-2010, #CA-270385
www.ScionHasArrived.com www.Bruker.com
Petrochemical
Gas Chromatographs
Analyzer Solutions
Solutions
Bruker has the experience and know-how to
provide pre-configured gas chromatographs
that are ready at power up to handle your key
applications. Our long experience in designing,
configuring and manufacturing complete sys-
tems, with all their analytical benefits, ensures
you get the solution that’s right for you. With
a host of standard solutions configured to
meet the performance specifications outlined
in international methods, and the capability to
produce unique, tailor-made solutions, we have
the answer that you seek.
Bruker Analyzer Solutions

Benefits
Included with all

Bruker Analyzer
solutions:
All Hardware
Software (incl. special ”plug-ins”
where appropriate)
Pre-Installed methods
Bruker configures and tests GC hardware Test Chromatograms
and software according to widely used Installation/ Validation Data
industry standard methods (e.g. ASTM,
Trouble Shooting Guide
UOP, EN, ISO, GPA,…), to save its clients
User documentation customized for
time and to ensure confidence in results.
the specific method
Solutions are configured to meet the per-
formance specifications outlined in the set
method itself.
Bruker Simulated Distillation Analyzers
Carbon Number
1 2 3 4 5 6 10 16 20 30 44 60 80 120
000000
ASTM D3710 Gasoline
Key Benefits Include:
ASTM D7096 Gasoline including ethanol
ASTM D5399 Hydrocarbon solvents
ASTM D2887
ASTM D5442
Petroleum fractions
Petroleum wax
Accurate boiling point distribution
ASTM D7213
ASTM D6417
Medium petroleum distillates
up to 750°C
ASTM D6352 Medium and heavy petroleum distillates
ASTM D5307 Crude petroleum
ASTM D7500 Medium and heavy petroleum distillate fractions Integrated standard test methods,
ASTM D7169 Crude oils and residues
DIN 51581‐2
applications fully comply with ASTM,
DIN 51435 Medium and heavy petroleum distillates IP, DIN and ISO standard test methods
IP 406 Petroleum products
IP 480 Middle distillates and lubricating base oils
IP 507 Vacuum distillates and residues Complete, single vendor solution
IP 545 Crude oil
EN 15199‐1
EN 15199‐2
Middle distillates and lubricating base oils
Vacuum distillates and residues
Complete control from initial setup
EN 15199‐3 Crude oil to final report
ISO 3924 Petroleum fractions
0
‐200
‐328
‐100
‐148
0
32
100
212
200
392
300
572
400
752
500 600
932 1112
700 750
1292
800 °C
1472 °F
ASTM D86 and ASTM D1160
correlation
Table 1: Overview Simdist methods.

Built-in Reports:
A gas chromatographic (GC) technique, Bruker’s SimDist software provides a

Simulated Distillation (SimDist) wide variety of report options to meet
reproduces the physical distillation of specific requirements including;
petroleum materials and products by
determining boiling point distribution.
Bruker’s range of Simulated Distillation Chromatogram with merged corrected
Analyzers are designed to meet all blank analysis and IBP/FBP marks
industry standard methods, Bruker’s versus retention time
analyzer software includes both ASTM
Boiling point versus percentage
D86 and ASTM D1160 correlations.
of sample
Bruker’s highly automated GC,
CompassCDS Chromatography Data Table and plot with retention time
Handling Software, and integrated versus boiling point
SimDist software are also designed to
meet worldwide industry standard test D86 and D1160 correlations
methods.
DIN Noak and motor oil
volatility reports
Table with cut points and fractions plus

residue analysis with recovery
calculation up to C120
436-GC with Sampler

Hydrocarbon Analysis by
Group (PIONA+™)
Characterization of Engine Fuels by Reduced Analysis Time and Figure 1: Traps are easily
Hydrocarbon Group Type Increased Sample Throughput accessible and do not require
Efficiency through the Use of any tools to install or replace.
Bruker’s PIONA+™ Analyzer is a highly “Concurrent” Heating
flexible GC analysis platform to obtain
comprehensive characterization and A unique aspect of the design of the
quantitative information, including Bruker PIONA+ system is the ability to
hydrocarbon group types, oxygenates independently heat the individual traps.
and carbon number distribution for spark
ignition engine fuels. This has 2 major operational benefits:
Key Analyzer Capabilities: Enhanced elution integrity for wide

range sample
Unparalleled operational flexibility Significantly Reduced Analysis Time
Compliant with established standards

The independent and concurrent heating
A complete and fully integrated design permits greater trap control and
solution benefits in improved elution integrity of
the component groups e.g napthene, iso-
A powerful analyzer, easy to use, paraffins and n-paraffins even for a wide
generating outstanding analysis results range sample (C4 - C11). In addition, only
day after day a single Molsieve column temperature
cycle is employed thus reducing the
The PIONA+ Analyzer performs a analysis time by almost half allowing a
complete analysis (as described in “fast” PIONA mode of operation (see
ASTM D6839 and similar methods) figures 2 and 3).
and provides unprecedented analytical
flexibility and simplified operation By employing this technique, sample
through the use of a novel approach throughput can be nearly doubled
to column/trap heating and exchange compared to systems that do not offer
(Figure 1). this unique capability.
N = Napthene (cylcic Paraffin)

iP = iso Paraffin
nP = normal Paraffin
A = Aromatic
pN = polyNaphthene
cO = cylic Olefin
iO = iso Olefin
nO = normal Olefin
N+iP(6-10) A6/A7 >200C nP(6-11) A8/A10 >200C cO+iO(6-8) nO(6-7) N+iP+nP(5-11) A6/A7 >200C cO+iO+nO(6-8) A8/A10 >200C
+pN +pN
0 30 60 90 120 150 Min 180 0 30 60 Min 90
Figure 2: Chromatogram of a test mix in Figure 3: Chromatogram of a test mix using

conventional PIONA mode. (Analysis Time - concurrent heating in “fast” PIONA mode.
180mins) (Analysis Time = 95mins)
Determining Total Olefin Content
Is Now Practical
The stability, sample loading and lifetime for all of the critical chromatographic
components have been improved and optimized in the Bruker PIONA+ Analyzer.
Of special and particular note is the improvement in increasing the sample loading
capacity of the “olefin” trap. As a result, it is now possible to analyze streams with
olefin content as high as 35-40% or more. This makes it practical to employ a single
analytical method to obtain total olefin content (Table 2).
Table 2
Saturates Unsaturates
Carbon Cyclic Iso Normal Cyclic Iso Normal Aromatics Oxygenates Total
3 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
4 0.00 0.00 0.06 0.00 0.03 0.54 0.00 0.00 0.63
5 0.31 11.37 2.98 0.87 9.92 7.58 0.00 0.00 33.03
6 3.19 9.98 1.40 2.40 8.59 4.40 1.72 0.00 31.68
7 4.31 6.77 0.00 2.14 4.76 1.91 7.47 0.00 27.36
8 1.42 3.12 0.00 0.39 2.06 0.00 0.07 0.00 7.06
9 0.01 0.00 0.00 0.04 0.02 0.00 0.03 0.00 0.10
10 0.01 0.00 0.00 0.09 0.00 0.03 0.01 0.00 0.14
11 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
Total 9.25 31.24 4.44 5.93 25.38 14.46 9.30 0.00 100.00
Example weight % report for a Naphtha sample with high (46%) olefinic content; (highlighted in blue).
The analyzer design allows the operator

to conduct analyses in any one of a
number of different operational modes
including PNA, PONA, PIONA, O-PONA
and O-PIONA in standard and concurrent
heating configuration. The system is
compliant with established standard
methods (see adjacent array).
Detailed Hydrocarbon Analyzer
The DHA Analyzer is a complete high Complete Gasoline Chromatogram

resolution GC solution for the analysis of
hydrocarbons in petroleum streams. It is
capable of performing all of the standard
methods including ASTM D6729,
D6730, D6733, D5134, D6623, IP 344/
DHA “Front End” and “Fast DHA”.
Although each DHA analyzer is

configured, tested and certified at the
factory for a standard method specified
by the customer, the DHA software
permits the operator to utilize any of
the other popular standard methods as
well. And, because of the outstanding
performance and flexibility of the Bruker
GC and CompassCDS software design, Figure 4: The analysis of permanent gases and hydrogen using the Rapid RGA.
Bruker is able to quickly modify the
existing methods or add new ones if
required as a result of the on-going
dynamic industry standard processes.
Figure 5: Report selection output.

7 8 9 10
7 9
Peak Identification
10
1. Hydrogen 12 8
12
2. Carbon Dioxide
3. Hydrogen Sulfide
4. Oxygen
14 19
14 23
5. Nitrogen 19
24 25
26
18
21
26 11 13 15 16 1718 20 21
17 20 24 21
6. Carbon Monoxide 11
13
15
25 22
16 22
7. Methane 0 1 2 3 4 Min. 5
8. Ethane
0 4 8 12 16 20 24 Min. 28
9. Ethylene Figure 7: The analysis of light hydrocarbons using the Rapid RGA,
10. Propane with complete separation in less than five minutes.
11. Cyclo Propane Figure 6: The separation of light hydrocarbons
12. Propylene using the Standard RGA. 7
1
9
13. i-Butane
The source and composition of refinery Figure 8:
14. n-Butane 8 5
gases varies considerably. Measuring gas The analysis of

15. Propadiene
composition precisely and accurately is permanent gases
16. Acetylene
a significant challenge in today’s refinery 2 and hydrogen
17. t-2-Butene
operations. Bruker’s Refinery Gas using the Rapid
18. i-Butene 16
3
Analyzers (RGA) are designed to deliver 4

6
RGA.
19. c-2-Butene
superior, reliable results for a wide range 0 Min. 7 0 Min. 2
20. i-Pentane
of sources and analysis throughput
21. n-Pentane
requirements. Bruker Offers Two RGA Systems
22. 1, 3-Butadiene
23. Propyne to Meet the Widest Range of
Key Benefits: Analysis Requirements:
24. Butyne
25. C6+
26. Helium Pre-configured and tuned Standard RGA:
A three channel multi-valve design using
Standard methods including UOP 539, both capillary and packed columns.
DIN-51666 and ASTM D2163
Channel 1 - Analysis of permanent gases
Integrated micro-gasifier ensures
Channel 2 - Light hydrocarbons
complete vaporization of LPGs and
Channel 3 - Hydrogen.
high pressure samples to prevent
sample discrimination (option)
Total analysis time for all components in
Multi-channel approach 25 minutes.
Rapid RGA:
The Standard RGA packed columns
in the hydrogen and permanent gas
channels are replaced by micro packed
columns and installed in a separate
column oven. Key benefits of this
design are:
Flexibility
Reduced Analysis Time - 5mins

(with H2S - 7mins)
Increased Sample Throughput

Table 3: Standard RGA vs Rapid RGA.
Low Level Oxygenates Analyzer
75000
18
70000
65000
60000 7
3
55000 4
8
50000
45000
17
40000
19
35000
30000
5
25000
1 15
20000
12 16
15000
9 10
10000 13
14
6
5000 2
11
0
-5000
0 3 6 9 12 15 18 21 24 27
Min
Figure 9: Typical chromatogram showing a wide range analysis of a liquid sample stream.
The determination of sub to high ppm The system is comprised of a Bruker GC Peak Identification
levels of ethers, alcohols, aldehydes configured with gas and liquid sampling 1. Diethylether
and ketones in different hydrocarbon valves, two high performance capillary 2. Acetaldehyde
matrices is a recurring challenge in the analysis columns, digitally controlled 3. Ethyl tert. Butyl ether
petroleum refining and petrochemical pneumatics including a ‘fluidic’ switch 4. Methyl tert. Butyl ether
industry. The Bruker Low Level and Flame Ionization Detector (FID). 5. Diisopropylether
Oxygenates Analyzer is an easy to use An optional ‘pressure station’ can be 6. Propanal
solution to meet this challenge and is added to eliminate the possibility of 7. tert amyl methyl ether
according ASTM D7423. losing sample due to evaporation when 8. Propylether
analyzing LPG. The GC is controlled via 9. Isobutyraldehyde
The Low Level Oxygenates Analyzer the CompassCDS Chromatography Data 10. Butyraldehyde
is designed and optimized to quantify Handling Software, which acquires data, 11. Methanol
ppm and sub ppm levels of ethers (e.g. processes it and generates analyses 12. Acetone
DME, MTBE, ETBE, DIPE), alcohols (e.g. reports. 13. Isovaleraldehyde
methanol, ethanol, propanol), ketones 14. Valeraldehyde
(e.g. acetone, MEK) and aldehydes in 15. 2-Butanone
various hydrocarbon matrices. In general, 16. Ethanol
all oxygenated components with a boiling 17. 1-Propanol
point of up to 100°C can be analyzed and 18. tert Butyl alcohol
the sample can be a gas, LPG or liquid & Isobutanol
under ambient conditions with a final 19. 1-Butanol
boiling point up to 250°C.
Bruker 4815 GC Oxygenates Analyzer
The Bruker 4815 GC Analyzer provides The type and concentration of

a highly cost effective solution for the oxygenated compounds must be
analysis of oxygenates in gasoline, measured in reformulated gasolines
according to the widely used industry as part of ongoing product quality
standard method ASTM D4815. The assessment, and to confirm the
combination of Bruker’s reliable GC oxygenated components have been
hardware, powerful software and added in the correct amounts according
industry leading pre- and post-sales to regulatory requirements (e.g. California
support teams make this analyzer Air Resources Board).
package the most comprehensive
solution available today. ASTM D4815 is frequently chosen as the
standard method for the determination of
Oxygenated compounds can be present oxygenated compounds. Individual ethers
in various hydrocarbon matrices either and alcohols are quantified in gasoline
because they were purposely added (e.g. including: MTBE, ETBE, TAME, DIPE,
into gasoline), because they are naturally C1-C4 alcohols and tert-amylalcohol.
present, or formed during catalytic Individual ether components are
processes such as polymer production. measured from 0.1 to 20.0 mass %.The
In gasoline, oxygenated compounds are individual alcohols are measured from 0.1
added as ‘anti-knock’ agents to increase to 12.0 mass %.
the octane number and decrease
emissions by replacing organo-lead
compounds.
t-Butanol
iso-Propanol
Ethanol
n-Propanol
tert-Amyl Alcohol
sec-Butanol
Benzene
iso-Butanol
TAME
Methanol
n-Butanol
MTBE
DIPE
c
ETBE
DME
456-GC with Sampler

0 2 4 6 8 10 12 14 Min 16
Figure 10 : Typical Chromatogram of the test sample.

Trace Impurity Analyzers
Sulfur Components in LPGs
Low level analysis of sulfur containing

components such as H2S, COS and
mercaptanes is extremely challenging
and a configured GC offers the solution.
Firstly, the system employs a micro-

gasifier enabling the direct coupling
of an LPG stream. Secondly, an inert
steel sample path ensures a trouble free
analysis of sulfur containing components
at low concentrations. Finally, a two
channel PFPD/ two column approach
permits the analysis of all components Figure 11: Sulfur components in propane, BR-1 column.
of interest in one run whatever the LPG
matrix. Two differing columns ensures
quenching of PFPD signal by the matrix
is overcome and full sulfur component
analysis is achieved. Figures 11 and
12 show chromatograms obtained in a
propane matrix and illustrates the novel
benefits of the 2 channel approach.
Permanent Gases in LPGs
Impurities such as CO, CO2, H2, O2 and

Figure 12: Sulfur components in propane, BR-Q PLOT.
N2 need to be determined at low levels
in LPGs. Complete separation of these
components is done using a two channel
single detector (PDHID) system. The
GC employs a permanent gas channel
for analyzing H2, O2, N2, CH4 and CO
and a specific second channel for the
analysis of CO2. A gasifier is used as a
sample introduction device thus giving
the capability of handling LPG samples
C2 through C4.
Detection limits are at the ppb level Figure 13: LPG sample.
(Figure 13), depending on the component
of interest.
Total Characterization of
Ethylene Impurities
For a total characterization of impurities GC-1
in ethylene and also propylene six GC H2 Channel (TCD)
channels are required. By coupling two O2 /N2 Channel (TCD)
Bruker GCs with three channels each, CO, CO2 Channel (Methanizer/FID)
a comprehensive solution is available
for analyzing these components. The GC-2
channels used in this analyzer are Light Hydrocarbon Channel (FID)
analytical tools principally developed for Oxygenates Channel (FID)
the determination of different gases in Sulfur Channel (PFPD)
various hydrocarbon types of gaseous
matrices. The results (see figures 14 to 19)
demonstrate how this 6 channel
system is perfectly suited for the total
characterization of ethylene and its
impurities.
Figure 14: CO, CH4 and CO2 on GC-1. Figure 15: H2 on GC-1. Figure 16: O2 and N2 on GC-1.
Figure 17: Light hydrocarbons on GC-2. Figure 18: Oxygenates on GC-2. Figure 19: Sulfur components on GC-2.
Natural
Gas Analyzer
A Family of optimized GC Solutions
Gas Chromatography
Bruker Natural Gas Analyzers
Natural gas is bought and sold as a bulk commodity with price based on its energy
content. It is very important for all stakeholders in the natural gas supply and
consumer chain to accurately determine the heating value of their
streams. Bruker offers a full range of GC based solutions for the analysis of natural
gas. The analyzer family is designed to offer superior results through the use of
industry proven hardware, software, optimized columns and consumables, and is
backed by a team of global sales and support specialists.
Key Benefits include:
A complete range of natural gas Operational procedures are fully

analysis (NGA) solutions. Bruker documented. All Bruker NGA analyzers
offers many different NGA gas not only incorporate proven GC
chromatography analyzers to meet the hardware and software, but arrive with
broadest range of stream sample types the pre-loaded analysis method(s)
and throughput needs, whether the and documentation specific to the
analysis is conducted in a laboratory, application.
at-line or in the field.
Comprehensive single-vendor
Easy to operate, powerful GC solutions. solution. Bruker is proud to provide
Bruker’s GC with CompassCDS complete solutions. The hardware,
Chromatography Software, form a software, application optimization,
powerful combination and do not documentation, installation and
require a high degree of skill to be used performance verification are all
successfully. delivered by Bruker.
Flexibility to analyze natural gas,

liquified petroleum gas or natural gas
liquids (NGL). Bruker’s GC based NGA
analyzers can be configured to measure
the composition of LPG or NGL
streams through the use of specialized
sample conditioners, ensuring sample
integrity is constistently maintained.
Figure 1: The Bruker GC based

Natural Gas Analyzer.
Solutions for Natural Gas Analysis
Gas chromatography offers a proven

2 Peak Identification
means to determine the composition and 1. Air
heating value of natural gas and related 2. Methane
streams quickly and cost effectively. 3. Carbon dioxide
4. Ethane
Bruker’s natural gas analyzers (NGAs) are 5. Hydrogen sulfide
standard ‘turnkey’ systems pre-configured 1 6. Propane
and tuned at the factory to ensure their 7. i-Butane
8. n-Butane
compliance with standard methods used 9. i-Pentane
to determine the heating value of natural 10. n-Pentane
gases and related streams. They can 11. n-Hexane
also be specially configured to determine 4 6

other components of interest (eg. sulfur 7 8
compounds), to ensure suitability for
use in downstream processes. The 3
analyzers are based on the Bruker GC gas
5 9 10 11
chromatograph platforms and Bruker’s
CompassCDS Chromatography Software.
All systems employ a proven and optimized Min
0 4 8 12 16 20 24 28 32
multi-channel/multi-dimensional approach
to determine the heating/calorific value of
Figure 2: Chromatogram of natural gas sample from ‘System A’.
natural gas, as well as quantify individual Up to 10 Min TCD signal, 10 to 31 Min FID signal.
components.
Bruker offers several NGA systems to
meet the widest range of analysis needs.
Basic NGA (System A) Carrier 1 IN

Packed Injection
Port
This is the simplest of all available natural Sample loop
Sample IN
gas analysis systems. As shown in Sample OUT
V-2
Figure 3, the system employs a single 8

1
2
valve column designed for simplicity, a 7
V-1
3
Thermal Conductivity Detector (TCD) and 6
4
Flame Ionization Detector (FID). The TCD is 5
used for the determination of O2, N2, CH4,

CO2 and Ethane, while the FID, connected Flame out Porous
buffer Polymer
in series, determines hydrocarbons in low Column
concentrations, i.e. C3-C5 and C6+ back-

flushed grouping peak (late back-flush). A
single unheated 4 port Liquid Sampling
TCD Reference
Valve (LSV) is available for LPG type
samples. Figure 3: System
TCD FID
configuration schematic
diagram for Natural Gas
Analyzer ‘A’.
Natural Gas Analysis
NGA/Natural Gas Liquids (System B) of hydrocarbons and CO2. The Non-Polar
columns are set up for early back-flush,
This system is optimized for the analysis of
which optimizes sensitivity while reducing
natural gas or de-methanized hydrocarbon
run time (from 25 minutes using the
matrices. For natural gas, the components
system ‘A’ configuration to less than 15
of interest are typically oxygen, nitrogen,
minutes) (Figure 5). The System „B“ is
carbon dioxide, methane, ethane, propane,
extendable with two standard options. For
butane, isobutane, pentane, hydrogen
the analysis of de-methanized liquefied
sulfide, and C6+ as a composite peak. For
natural gas distillates (i.e. propane, butanes
de-menthanized streams (liquid natural gas)
and pentanes) an automated 4 port Liquid
the components of interest are typically
Sampling Valve is available to inject the
carbon dioxide, ethane, propane, butane,
sample as a Liquid. For the analysis of
iso-pentane, hexane, and C7+ as
hydrogen and helium in natural gas a He/H2
a composite peak.
channel is available as extra GC channel.
The system is configured with a 10 and 12
port valve (a third liquid sampling valve is
added if liquid streams are to be analyzed) Peak Identification
and three analysis columns connected 3 5 1. Oxygen
to TCD and FID detectors (Figure 4). The 2. Nitrogen
3. Methane
system simultaneously injects the stream 2 4. C6+ composite
onto two column systems, a Molsieve 5. Methane
7 6. Carbon dioxide
column for the determination of O2 and
7. Ethane
N2 without the use of coolants, and short/ 8. Hydrogen sulfide
long Non-Polar columns for the analysis 9. Propane
10. i-Butane
11. n-Butane
12. i-Pentane
9
1 13. n-Pentane
Sample loop
Sample IN
V-3
Sample OUT 6 11
10
1
2
10
Carrier 1 IN 9
3
8
V-1
Carrier 2 IN 4 13
7
4
8 12
5
6
Sample loop Min

0 2 4 6 8 10 12 14
Molsieve
Column
Figure 5: Chromatogram of natural gas samples from ‘System B’.
1
Note separation of oxygen and nitrogen.
12
2
11 Vent
3
NV-1
10
V-2 TCD
4
9
Reference
5
8
7 6
Non-Polar
Pre- Non-Polar
Column Column
Flame-out
Buffer
FID TCD
Figure 4: System configuration schematic for

Natural Gas Analyzer ‘B’.
“Rich” Natural Gas Analysis
NGA/Natural Gas Liquids - Gas sample OUT
Extended Analysis (System C) Gas sample IN

Carrier 1 IN V-3 Carrier 2 IN
This system is specifically designed to 14 1

2
Sample loop
Sample loop
13
analyze ‘rich’ natural gas or natural gas 12

3
4
11
V-1
liquid streams by separating all 10

9
6
5
8 7
hydrocarbon components up to C16. As

with System B, it separates and quantifies
Carrier 3 IN
oxygen and nitrogen, as well as measuring
Capillary
Sample loop
hydrogen sulfide down to ~100 ppm. Injection
Port
Delay
column
1
Split vent
The system is configured with a 14 port 6
2
Porous V-2
valve and 6 port valve. The 14 port valve Polymer 5
3
Molsieve
Column
Column
enables the system to introduce the 4
Non-polar
Capillary
Vent
sample stream simultaneously to three Column
independent columns with automated

detector switching which provides high
TCD FID
sensitivity detection of all components
TCD Reference
of interest. The valves are installed in the
multi-valve oven for flexible operation of
Figure 7: System schematic for Natural Gas Analyzer ‘C‘
the conventional column oven. Two of the
sample paths flow onto Molsieve and
porous polymer columns to separate to the TCD detector while components
oxygen, nitrogen and carbon dioxide, remaining on the Molsieve column are
ethane, methane, ethane and H2S, and the flushed to vent. If natural gas liquids are to
other via a ‘splitter’ onto a high be analyzed, a third valve (liquid sampling)
performance non-polar capillary column to is added to the configuration described
separate the hydrogen components above. For natural gas containing hydrogen
up through C16. The 6 port valve is used to and helium a He/H2 channel is configured
direct the separated components fraction additionally.
Peak identification
1
1. Propane 12. 2,2,4-Trimethylpentane Peak identification
2
2. iso-Butane 13. n-Heptane 1. O2 /Ar
3 3. n-Butane 14. Toluene 2. N2
3 4
4. neo-Pentane 15. n-Octane 3. O2 /Ar/N2
5 5. iso-Pentane 16. Ethylbenzene 4. Methane Peak identification
1. O2/Ar
6. n-Pentane 17. m- + p-Xylene 5. CO2 2. N2
6 3. O2/Ar/N2
7. Cyclopentane 18. o-Xylene 6 6. Ethane 4. Methane
5. CO2
8. n-Hexane 19. n-Nonane 2 7. H2S 6. Ethane
7. H2S
9. Methylcyclopentane 20. 1,2,4-Trimethylbenzene
10. Benzene 21. n-Decane
11. Cyclohexane
8 1
5
12
9 11 13
7
16 17
4 18 20
10
14 7
15 19 21
0 1 2 3 4 5 6 7 Min. 8 0 2 4 6 Min 8
Figure 6: Chromatogram showing natural gas sample from ‘System C’ FID Figure 8: Chromatogram showing natural gas sample from
channel. Note individual separation of C6+ components ‘system C’ TCD channel.
Base Natural Gas Analysis Systems
Bruker GC Bruker GC Bruker GC

Analyzer Characteristic System ‘A’ System ‘B’ System ‘C’
Gas Components Measured
O2, N2, CO2, CH4 YES YES YES
Ethane, Propane, Butane, Iso-butane, Neo-
YES YES YES
pentane, Pentane, Iso-pentane
C6+ as a composite peak YES YES YES
C7+ as a composite peak YES YES YES
O2/N2 Separation YES(1) YES YES
(3)
He/H2 Separation NO YES YES(3)
Max hydrocarbon number speciated C6 C7 C16
(4 or 5) (4 or 5)
LPG YES YES YES(4 or 5)
De-methanized natural gas/natural gas liquids NO YES(4) YES(4)
Typical analysis to analysis repeatability % <1.0 <1.0 <1.0
RSD
Analysis time 30 min 14 min 15-20 min
Standard Gas Methods
IP-345 YES
GPA 2172
GPA 2177 YES
GPA 2261 YES
GPA 2286 YES YES
GPA 2186 YES YES
ASTM D5504 YES(5)
ASTM D6228 YES(5)
Natural Gas Calculation Methods
ISO 6974 Contact Bruker, several configuration
possibilities
ISO 6976
GOST-22667
(1)
Requires liquid nitrogen or liquid CO2 oven cooling
(2)
Requires 3rd dedicated channel
(3)
Requires additional channel including valve, columns and TCD
(4)
Requires LSV to be additionally installed
(5)
This method is specifically for sulfur components in natural gas,
therefore a sulfur selective detector must be used such as a PFPD
Sulfur components in Natural Gas
CompassCDS
Figure 9: Natural Gas Report - ‘System C’

generated using Bruker’s CompassCDS
Chromatography Data Software
There are several methods specifically

THT
used for the analysis of sulfur
components in natural gas, e.g. ASTM
D5504 and ASTM D6228. Bruker’s
natural gas analyzers can be modified
to measure sulfur components through
the addition of an extra, fully inert H2S COS
channel, dedicated for the determination CH3SH

of lowlevel sulfur components only. CH3CH2SH
This sulfur channel will be equipped

TBM
with the Pulsed Flame Photometric
Detector (PFPD), a sulfur specific 0 4 8 12 16 20 Min 24
detector. The systems can also be
configured and tested exclusively for the
analysis of sulfur components as per
standard methods, or enterprise specific Figure 10: Detection of trace level sulfur components using the 450-GC, specially
treated with Inert Steel surface deactivation and Pulsed Flame Photometric
requirements. Detector (PFPD).
analyses.

productivity
improved up-time
DHA Analyzer Family
Optimized Solutions for Detailed
Hydrocarbon Analysis
Gas Chromatography
The DHA Analyzer is a complete high resolution
gas chromatography solution for the analysis
of hydrocarbons in petroleum streams. It
is capable of performing all of the standard
methods including the analysis of light
petroleum streams and crude oil light end.
Key Benefits include:
Compliant with all industry Save time

standard methods Easily generate reports with a few
Be confident using Bruker’s DHA mouse clicks and reduce analysis
Analyzers, which are configured in time using “Fast DHA”, increasing lab
accordance with all the established productivity.
standard methods including ASTM
D6729, D6730, D6733, D5134, Single vendor solution
IP 344/DHA “Front End”and “Fast DHA”. Bruker’s GC analyzers are built and
tested at Bruker’s factory, as well as
Complete and fully integrated installed and performance-verified
solution on-site by Bruker trained and certified
DHA Analyzers come complete with engineers. Rest assured that our
everything you need to be up and analyzers can meet or exceed your
running quickly. needs throughout the instrument’s
lifetime.
Powerful and easy-to-use analyzer
With relatively little training, operators
can generate outstanding analysis
results day after day.
DHA Analyzer Family
Detailed hydrocarbon analysis is often the the presence of naphthenes is more

preferred technique to fully characterize common in Naphtha than in Reformate
petroleum streams. The technique is where aromatics are more dominant. The
based on the identification of individual DHA analyzer allows presetting a sample
components using high performance, high type within the DHA method. By setting a
resolution capillary gas chromatography. component identification preference for a
coelution, the most likely main component
Software Ensures Accurate is assigned and sample characterization
Identification is improved. The software also allows
defining additional sample presets in case
To successfully apply gas chromatography
new sample types become available.
to detailed hydrocarbon analysis (DHA) the
analyzer must be able to correctly identify a
large number of components (many eluting Component Present in:
very closely to one another) in a complex KRI or LRI* Component Hydrocarbon Type
Reformate Naphtha Alkylate
chromatogram. The identification is based
1,1,3-Trimethyl cyclopentane Naphthenic C8 - + -
on a comparison of their individual retention 721.4
2,2-Dimethylhexane iso paraffin C8 ++ + -
index values to those in a pre-established
Toluene Aromatic C7 +++ + -
database. Therefore, it is extremely 751.1
2,3,3-Trimethylpentane Iso paraffin C8 - + ++
important that the analyzer functions in a
o-Xylene Aromatic C8 +++ + -
highly repeatable manner. 877.9
1,1,2-Trimethylcyclohexane Naphthenic C8 - + -
Due to the high number of possible * KRI = Kovats REtention Index. LRI = Linear Retention Index.
components in hydrocarbon streams,
even though analyzed on a high resolution Table 1: For some sample streams a single component assignment is possible due to
column, it cannot prevent coelution the assumed absence of one of the coeluting components.
of some hydrocarbons. However, the
presence of certain hydrocarbons depends
on the type of sample stream. For example
Figure 2: Detailed hydrocarbon

analysis of a reformate sample
showing aromatics identification
according ASTM D6730
Standard Methods
Selecting Individual Peaks and

Updating the Database
The DHA software includes a Peak
Select and Database Update function to
make identification of unknown peaks as
straightforward as possible. The system
automatically provides the operator with
detailed comparative retention index
Figure 5: Choosing a preferred standard method is easy with the DHA software
information for each “unknown” peak
including a highlighted “best fit” indicator,
making it easy for the operator to determine
the ID.
Figure 6: Choosing report options is simple
Although each DHA analyzer is configured,

Figure 3: Assigning custom peak matching criteria
tested and certified at the factory for
is easy.
a standard method specified by the
customer, the DHA software permits
the operator to utilize any of the other
popular standard methods as well. And,
because of the outstanding performance
and flexibility of the Bruker GC and
CompassCDS software design, Bruker
is able to quickly modify the existing
methods or add new ones if required as a
result of the on-going “dynamic” industry
standard processes.
Powerful Reporting is Built-in

Figure 4: DHA provides an easy-to-use graphical Bruker’s DHA software includes several
means to select peaks and update the database report options to accommodate the
standard methods and/or to meet the
Integrated Standard Test Methods customer’s special needs. These include:
Bruker’s DHA analyzers are compliant with Carbon number distribution
the following methods: PIONA report; (weight and volume
percentage by hydrocarbon group)
ASTM D6729 ASTM D5134
ASTM D6730 “Fast” DHA Physical properties calculations;
ASTM D6733 IP 344 “Front end“ specific gravity and molecular weight
True distillation profile
RON/MON specification
Reduce Sample Analysis Time With “Fast DHA”
Figure 7: These chromatograms illustrate the decreased analysis time using the “Fast DHA” method. Chromatogram of a naphtha sample
run on a 40 m X 0.10 mm X 0.2 µm film BR-DHA using the “Fast DHA” method (left). Chromatogram of the same sample, but run on a 100
m X 0.25 mm ID X 0.5 µm film BR-DHA column using standard method D6729 (right). Note reduced analysis time from ~80 minutes to ~20
minutes; almost four-fold.
DHA Analyzer Includes These Key

Components Bruker compassCDS
ASTM D6730
Bruker GC high performance gas Sample Name

Sample Preset
Reformate
Reformate
Vial
Sample Wt
chromatograph equipped with: Instrument Demo ISTD Wt

DHA Method
1,0000
ASTM D6730.dha
Database D6730_v4.mdb
- Split/splitless capillary injection port Data File

Method
DHA\D6730 DHAX\6730_reformate.DATA
D6730
Description Reformate
- High performance capillary column # Time Index Name Grp Area Mass% Vol%
15 19,967 566,2 2-methylpentane iP 4,787E-003 2,425 2,998
(dependent on specified method 16

17
20,133
21,105
568,4
580,8
t-4-methyl-2-pentene
3-methylpentane
iO
iP
2,903E-005
3,717E-003
0,014
1,883
0,017
2,289
18 21,588 587,0 2-methyl-1-pentene iO 4,616E-005 0,023 0,027
on order) 19
20
21
21,680
22,615
22,862
588,1
600,0
602,3
1-hexene
hexane
t-3-hexene
nO
nP
iO
1,463E-005
4,991E-003
3,595E-005
0,007
2,528
0,018
0,009
3,097
0,021
- Flame ionization detector (FID) 22

23
24
23,087
23,292
23,538
604,3
606,1
608,3
t-2-hexene
2-methyl-2-pentene
c-3-methyl-2-pentene
iO
iO
iO
5,038E-005
9,137E-005
5,101E-005
0,025
0,045
0,025
0,030
0,053
0,031
25 23,908 611,7 iO 2,701E-005 0,013 0,016
- Full electronic flow control (EFC) of
c-2-hexene
26 24,475 616,8 3,3-dimethyl-1-Pentene iO 8,013E-005 0,034 0,039
27 24,788 619,6 2,2-dimethylpentane iP 6,316E-004 0,319 0,380
28 24,968 621,2 methylcyclopentane cP 1,058E-003 0,522 0,563
all gases 29
30
25,407
25,867
625,1
629,3
2,4-dimethylpentane
2,2,3-trimethylbutane
iP
iP
6,777E-004
9,457E-005
0,342
0,048
0,411
0,056
Bruker compassCDS
ASTM D6730
31 27,080 640,2 4,4-dimethyl-1-pentene iO 1,411E-005 0,007 0,008
State of the art backflush capabilities for 32

33
27,312
27,480
642,3
643,8
1-methylcyclopentene
Benzene
Sample Name
Sample Preset
cOReformate 4,850E-005
Ar Reformate 1,309E-002
0,023
6,002
0,024
Vial
5,529
Sample Wt
34 27,947 648,0 3,3-dimethylpentane Instrument iP Demo 6,359E-004 0,321 0,375
ISTD Wt 1,0000
the IP 344 “Front End“ method 35

36
37
28,365
28,673
29,293
651,8
654,5
660,1
cyclohexane
t-2-methyl-3-hexene
4-methyl-t/-c2-hexene
Database
cP
iO D6730_v4.mdb
6,417E-005
2,401E-005
iO DHA\D6730 DHAX\6730_reformate.DATA
4,785E-005
0,032
0,012
0,024
0,033
DHA Method
0,014
0,027
ASTM D6730.dha
Data File
CP-8400 or CP-8410 automatic liquid 38

39
40
29,448
29,617
29,938
661,5
663,0
665,9
2-methylhexane
2,3-dimethylpentane
1,1-dimethylcyclopentane
Method
Description
iP
cP
D6730 4,203E-003
iP Reformate 1,576E-003
4,852E-005
2,122
0,796
0,024
2,529
0,922
0,026
41 30,483 670,8 iP 5,230E-003 2,640 3,099
sampler
3-methylhexane
42 30,952 675,0 3,4-dimethyl-c-pentene-2 iO 2,415E-005 0,012 0,014
43 31,263 677,8 c-1,3-dimethylcyclopentane cP
Physical Properties 8,679E-005
Report 0,043 0,046
44 31,622 681,0 t-1,3-dimethylcyclopentane cP 8,650E-005 0,043 0,046
CompassCDS for system control, 45

46
31,780
31,972
682,5
684,2
3-ethylpentane
t-1,2-dimethylcyclopentane
Reid Vapor Pressure
Liquid Density
iP
cP
Research Octane Number
5,386E-004
1,315E-004
0,272
2.4
0.8133
0,314
0,065 106.9 0,070
psi at 100 °F
g/ml (15 °C)
47 32,177 686,0 1-heptene nO

Motor Octane Number 1,544E-005 0,008 97.0 0,009
data acquisition and report generation 48

49
32,295
33,008
687,1
693,5
2-ethyl-pentene-1
c-3-methyl-3-hexene Nett Heating Value
Bromine Number nO
iO
Gross Heating Value
iO
2,878E-005
3,249E-005
0,014 44160.50,017KJ / Kg
0,016 41793.00,019KJ / Kg
50 33,493 697,9 t-3-heptene 5,168E-005 0,026 3.2 0,030
CompassCDS based DHA application 51

52
53
33,725
34,057
34,215
700,0
701,5
702,3
heptane
3-methyl-c-hexene-2
3-methyl-t-hexene-3
PIONA Report nP in Mass%
iO
Paraffins
iO
Paraffins
4,324E-003
1,384E-004
Paraffins
5,122E-005
Olefins
2,183
0,068
Aromatics
0,025
2,580
0,077
Unknowns
0,029
Total
Cyclo Iso Normal
software 54
55
56
34,473
34,710
35,162
703,5
704,6
706,8
t-Heptene-2
3-ethyl-2-pentene
c-Heptene-2
C4
C5
C6
iO
0,00
iO
0,32
iO
0,55
0,02 3,933E-005
0,18
2,12 2,443E-005
1,29
5,31 7,226E-005
2,53
0,000,019 0,00 0,022
0,080,012 0,00 0,014
0,210,036 6,00 0,041
0,00
0,00
0,00
0,20
3,81
14,61
C7 0,21
iO 6,86 5,939E-005
2,18 0,380,029 20,99 0,034 0,00 30,62
Computer/monitor
57 35,763 709,6 2,3-dimethyl-2-pentene
C8 0,23 1,98 0,74 0,63 24,54 0,00 28,12
C9 0,00 0,47 0,18 0,00 10,78 0,00 11,43
Date Printed 20-7-2012 13:45:22 C10 0,00 0,00 0,01 0,00 10,40Page 3 of 5
0,00 10,42
Created by DHA Reporter - Compass CDS
0,003.9.5
Pre-loaded standard methods

C11 0,03 0,00 0,00 0,20 0,00 0,23
C12 0,00 0,00 0,00 0,00 0,46 0,02 0,48
Heavy 0,00 0,00 0,00 0,00 0,00 0,10 0,10
Total 1,31 16,79 7,12 1,29 73,37 0,12 100,0
Factory test PIONA Report in Volume%

Paraffins Paraffins Paraffins Olefins Aromatics Unknowns Total
Cyclo Iso Normal
Reference chromatogram C4
C5
C6
0,00
0,34
0,60
0,02
2,76
6,53
0,25
1,67
3,10
0,00
0,10
0,25
0,00
0,00
5,53
0,00
0,00
0,00
0.28
4.86
15.99
C7 0,22 8,09 2,58 0,44 19,53 0,00 30.85
Reference standard for use in conducting C8

C9
C10
0,25
0,00
0,00
2,27
0,53
0,00
0,85
0,20
0,02
0,70
0,00
0,00
22,85
10,02
9,63
0,00
0,00
0,00
26.91
10.76
9.64
on-site performance verification C11

C12
Heavy
0,00
0,00
0,00
0,03
0,00
0,00
0,00
0,00
0,00
0,00
0,00
0,00
0,17
0,39
0,00
0,00
0,02
0,11
0.20
0.41
0.11
Total 1,41 20,22 8,66 1,48 68,11 0,13 100,0
Date Printed 20-7-2012 13:45:22 Page 1 of 5

Created by DHA Reporter - Compass CDS 3.9.5
Figure 8: Physical properties and detailed hydrocarbon report

analyses.

productivity
improved up-time
Bruker Daltonics
MALDI Biotyper
Microorganism Identification and Classification
MALDI-TOF
think forward
Identify Microorganisms in Minutes
Searching for the culprit?

Fast and reliable species-specific identi-
fication of microorganisms, like bacteria,
yeasts, or fungi, is mandatory in clinical
routine monitoring, food processing quality
control or environmental and taxonomical
research. Benefit from ultra-fast analysis
time and a simple consistent analysis
workflow with high differentiation power.
The molecular fingerprint

Discover bacteria’s identity by its own
unique molecular composition. In a
MALDI-TOF mass spectrometer, proteins
and peptides are arranged in a spectrum
with increasing mass – revealing a
characteristic peak pattern, even for
reliable differentiation of species:
The individual fingerprint.
Sample amount is not critical

The sample material can be a single
colony from overnight culture or a centri-
fuged portion of a liquid culture. Biological
material is prepared as smear or extract
respectively, representing millions of cells
roughly, although a minimum of 10 5 cells
can be measured accurately on a standard
MALDI sample target plate.
In One Simple Step
Ease of operation:
Real-Time analysis
Single bacterial colonies or other
biological material can be directly
applied to a MALDI sample target
Ps. mendocina DSM50017 |
and introduced to a FLEX series
Ps. oleovorans B396 | MALDI-TOF mass spectrometer.
Ps. fluoreszens B340 |
Ps. veronii DSM11331 | Spectra acquisition is completed after
Ps. putida DSM291 | a few minutes and data evaluation is
Ps. putida B401 |
directly linked to the measurement.
This simple and exclusive workflow
is adequate for most microorganisms,
avoiding the need to perform gram-
staining, oxidase- or any other tests.
Sophisticated instrumentation
With robust and reliable Bruker
Daltonics FLEX series instrumentation,
fast measurements with high
reproducibility are possible. The high
sensitivity of the instruments allows
identification of minimal sample
amounts. When employing the renow-
ned Bruker AnchorChipTM technology,
scientists need even less biological
material.
The molecular fingerprint is used
for pattern matching. Sophisticated
recalibration and statistical algorithms
allow robust and accurate identification.
Matching scores supported by color
Performance on the bench: the microflexTM
codes are used for ranking of results.
Ready-to-Go database
MALDI Biotyper integrates a ready-
to-use reference library comprising
thousands of individual species and is
growing continuously.
The system is open and can be easily
expanded by specific requests of the
user including data for ID of bacteria,
yeasts and fungi.
The Workflow: Only Little Interaction Required
1. Open MALDI Biotyper control wizard
Ps. mendocina DSM50017 |2. Define your project

Ps. oleovorans B396 |
Ps. fluoreszens B340 |
Ps. veronii DSM11331 |
Ps. putida DSM291 |
Ps. putida B401 |
3. Allocate your samples
It takes only minutes to

receive results on the screen.
You are only five mouse clicks
away from your results.
4. Initiate measurement
5. Receive results
Identification by peak-matching
Full Bioinformatics
Capabilities
Sub-Typing
In depth analysis of acquired data sets is
possible by applying different bioinforma-
tic tools like weighted pattern matching
algorithms, correlation and principal
component analysis.
Principal component analysis

Typical MALDI Biotyper 2.0 result window after off-line proces-
Principal component analysis, for sing of an unknown sample. Left: Peak matching window shows
example, is an excellent tool for fine the measured spectrum compared to the reference spectrum.
analysis of closely related isolates, Right window shows the hits in the database.
species or subspecies.
Multivariate analysis of pseudomonas species
Dendrogram generation
฀ ฀ ฀
Dendrograms are created literally within
seconds from all main spectra selected in
฀
one step.
฀
Creation of own reference files ฀
฀
Add your own references or create a ฀
complete new reference database by
฀
yourself straightforwardly with a few
additional mouse clicks. ฀
฀
฀ ฀
฀ ฀
฀ ฀ ฀
฀ ฀ ฀
3D scatter plot of the first three principle component scores for

six individual species of the genus Pseudomonas, Pseudomonas
veronii, P. fluorescens, P. mendocina, P. oleovorans, P. putida
species B401, P. putida species DSM291
Dendrogram generation
Clostridia dendrogram created with

MALDI Biotyper; finest differences
between species can readily be
evaluated. Data were generated in
cooperation with Prof. Krüger and
Dr. Grosse-Herrenthey, Institute of
Bacteriology and Mycology,
University of Leipzig, Germany.
Technical Specifications
Fields of application
Clinical routine monitoring, environmental and taxonomical research,
food processing quality control
Features identification, taxonomical classification, or dereplication of
microorganisms
Sample preparation / handling

Very simple and rapid preparation.
Very sensitive, amounts of roughly 10 5 cells are required
No initial assessment, each sample handled in the same way:
No gram staining, oxidase- or any other tests and conditions choice
Samples
Bacteria (gram+ / gram-), yeasts, fungi
Instrumentation
Fast measurements with high reproducibility on a microflexTM, autoflexTM
or ultraflexTM MALDI-TOF mass spectrometer
Software
MALDI Biotyper, COMPASSTM for FLEX series incl. flexAnalysisTM
Speed of analysis
Minutes from colony to result for a single sample,
approx. 1.5 hours from colony to result for 96 samples
For research use only. Not for use in diagnostic purposes.
“There was an urgent need

for a next generation techni-
que for the fast, convenient
and cost-effective identifica-
tion of microbial species.
Bruker Daltonics MALDI
to change specifications without notice. © BDAL 08-2008, LS-30/3
Biotyper is well suited for
this purpose as it fulfills
these requirements and as it
has proven to be very robust
and reproducible.”
Prof. Dr. Erko Stackebrandt,
Managing Director of DSMZ
www.bdal.com Bruker Daltonik GmbH Bruker Daltonics Inc.

Bremen · Germany Billerica, MA · USA
Phone +49 (421) 2205-0 Phone +1 (978) 663-3660
Fax +49 (421) 2205-103 Fax +1 (978) 667-5993
sales@bdal.de ms-sales@bdal.com
aurora M90
A new era in ICP-MS
ICP-MS
Your Partner in ICP-MS Solutions
Bruker continues to find new and novel ways

to meet your changing needs. As a leader in
elemental analysis you can be assured that
when you buy a Bruker ICP-MS, you’re buying
more than just an instrument. You’re buying a
relationship with one of the most respected and
experienced instrument companies in the world.
ICP-MS: It’s never been Easier
Bruker innovation, making Let Bruker Quantum work

ICP-MS easier for you
If you’ve ever wished that ICP-MS could If your goal is to spend less time
be simpler, wish no more. The aurora creating methods and optimising
M90 makes light work of it. No matter conditions, and more time running
what your requirements, with a Bruker samples, Bruker Quantum software
ICP-MS, you can tackle any application delivers. Enjoy accurate results in less
with ease. time with an intuitive yet flexible user
interface that takes the hard work out
Key benefits of aurora M90 include: of ICP-MS.
With auto-tuning of all instrument
Bruker’s patented high-efficiency 90
parameters, you can spend less time
degree ion optics and double off-axis
on instrument setup and more time on
quadrupole delivers exceptionally low
sample analysis. Saving you valuable
background noise and unmatched
time and money.
sensitivity – at more than 1 million
counts per second for 1µg/L. Fully automated, aerosol dilution
extends the high dissolved solids
Tunable from normal to high sensitivity,
tolerance of your ICP-MS allowing you
the aurora M90 is perfect for both
to directly analyze challenging samples
routine and research-grade appli-
without additional sample preparation.
cations – Flexibility at your fingertips.
The aurora M90 delivers industry
leading detection limit performance.
Collision/reaction interface (CRI)
technology makes setup of complex
cell systems a thing of the past.
Simply turn on the gas flow to remove
interferences. It’s that simple.
Featuring the only all-digital ICP-MS
detector, covering more than nine
decades of dynamic range in pulse
counting mode, the aurora M90
delivers fast and accurate multi-
element analysis from ultra-trace to
major levels in a single measurement.
Innovation you can trust
Patented 90 degree ion mirror and low Robust, high-efficiency plasma system
noise double off-axis quadrupole and patented Interlaced Coils break
provide industry leading sensitivity down your toughest sample matrices,
and background for lowest detection reduce matrix effects, and minimize ion
limits. energy spread for maximum sensitivity
and stability.
New and improved Collision Reaction
Interface (CRI II) provides even simpler All-digital extended range detector –
and more effective removal of trouble– means fewer dilutions, and longer
some interferences for interference- detector lifetime for greater productivity
free analysis of your samples. and lower running costs.
Our 90 Degree Reflecting Ion Optics Revolutionizes ICP-MS Performance

Interlaced plasma coils provide
Low noise quadrupole
exceptional plasma stability.
with curved fringe rods*
* US Patent 5,194,731
for ultimate efficiency and
low background. * US
Patent 6,762,407 B2
The ion mirror* reflects By keeping photons The aurora M90 CRI II*
the ion beam through 90 and neutrals away from provides faster, more
degrees, focusing the the mass analyzer, the flexible, interference-free
analyte ions into the qua- ion mirror greatly reduces analysis.
drupole with the highest background signal. *US Patent 7,329,863 B2
possible efficiency. *US Patent 6,614,021 B2
Fast, Flexible, Interference-Free
Analysis
Bruker is proud to bring you CRI II, now It’s that simple!
even simpler to use and more effective
No need for expensive or corrosive
at removing troublesome interferences
gasses such as ammonia or methane,
from your sample analysis.
so laboratory costs are reduced.
The CRI injects helium (He) and hydrogen
No additional cleaning as CRI forms
(H2) collision and reaction gasses directly
part of the cone interface, making
into the plasma as it passes through the
this interference management system
orifice of the skimmer cone.
maintenance-free.
This innovative approach suppresses
interferences before the analytes are Choose your analysis mode
extracted into the ion optics.
CRI II universal analysis mode provides
fast and accurate results for samples
routinely encountered across the wide
range of environmental and industrial
monitoring processes.
While multi-mode delivers the
ultimate in performance and flexibility
for any sample type including those less
Gas
routine in nature.
So if you need absolute confidence in
your results, no matter what the sample,
New clean
Original CRI II is the answer.
plasma Reaction zone
plasma jet
expansion
Gas
Mass scan with and without CRI
1.0E+07
1.0E+06
1.0E+05
c/s
1.0E+04
1.0E+03
1.0E+02
1.0E+01
1.0E+00
43 45 47 49 51 53 55 57 59 61 63 65 67 69 71 73 75 77
amu
Clean water Clean water with CRI
Dramatically reduce or eliminate troublesome plasma and

sample matrix based interferences, using CRI II.
The solution to your application needs
With a vast range of options to choose A range of autosampler and produc-
from, Bruker has the solution to your tivity-enhancing accessories provide
application. Choose from you with fast, unattended operation of
your ICP-MS.
CRI II for fast, accurate interference-
free analysis of your samples. The nitrox 500 accessory allows online
addition of nitrogen or oxygen gas
High sensitivity interface. Ideal for
to the plasma. Add nitrogen to lower
research applications on non-interfered
your detection limits on key elements
isotopes, pushing your detection
like As and Se. Add oxygen for routine
capability to levels never seen before.
analysis of organic solvents.
Make simple work of your most chal-
Fully-integrated speciation options for
lenging samples. Upgrade to the inert
the analyst wanting to know more
vacuum pump system for low
about their samples.
maintenance, high performance
ICP-MS operation. The aurora M90 is compatible with
a wide range of laser ablation
Clean room package is suited to
systems providing you with solution-
applications in the semiconductor
free analysis.
industry and provides an inert and
contamination-free environment for
ultra-trace analysis.
Application-specific sample introduc-
tion systems for routine analysis
of geochemical and petrochemical
samples.
Fully-integrated HPLC
interface makes light
work of speciation
applications.
Choose from a
range of accesso-
All PFA sample introduction ries to meet your
Boost the performance
kits allow direct analysis of sample handling
of your aurora M90 by
samples containing free HF requirements.
adding the nitrox 500.
acid.
Clean room package helps

semiconductor labs attain
clean room conditions for
ultra-trace analysis.
The Benchmark in Analytical
Performance
Quickly and reproducibly reduce

interferences
With CRI II you can quickly switch from
CRI to non-CRI, or between different
collision and reaction gases.
Multi-mode delivers the ultimate in
performance and flexibility for any
sample.
Determination of As in Cl
containing samples
Use CRI II in H2 mode to remove the ArCl
interference when determining As in high
chloride containing samples like blood,
serum and urine.
1 µg/L As in HCI matrix

Certified Measured
40 range µg/L value µg/L
35 27
Al 13 – 21.2 20
30 51
V 0.27 – 0.37 0.29
25
52
Cr 0.42 – 0.78 0.42
µg/L
20
56
15 Fe 404 – 460 mg/L 420 mg/L
75
10 As 1.4 – 2.2 1.8
5 78
Se 74.4 – 85.2 77.2
0 206, 207, 208
1% HNO3 0.1% HCI 0.5% HCI 1% HCI 2% HCI Pb 26.2 – 29 27.6
238
75
As with CRI 75
As without CRI
U 0.16 – 0.18 0.17
Comparison plots showing 1 µg/L spike recoveries Obtain accurate results in complex biological
for 75As without correction equations. matrices. Above, certified and measured values
ArCl interferences are removed, allowing accurate for Reference Whole Blood Seronorm WB1 show
trace level quantification of As. that trace and major levels can be determined with
accuracy and confidence using CRI II.
Unrivalled Performance
Element Units Measured Certified

24
Mg mg/kg 513 500
39
K mg/kg 3128 3100
44
Ca mg/kg 422 410
56
Fe mg/kg 39.0 40.7 ± 2.3
75
As mg/kg 0.024 (0.023)
78
Se mg/kg 0.026 (0.025)
114
Cd mg/kg 0.0270 0.0284 ± 0.0014
206-8
Pb mg/kg 0.182 0.187 ± 0.014
Maximum dynamic range for
food samples
Above, certified and measured values for brown bread reference
Determine toxic, essential and nutritional material BCR-191 showing accurate measurement from ultra-trace
elements in a single, all-digital measure- to major levels.
ment for optimum accuracy and precision.
Unequalled high sensitivity

performance
The aurora M90‘s unique high sensitivity
mode opens up a world of new
possibilities in research.
1000
100
10
Normal mode
mg/L
100
High sensitivity mode
10
µg/L
100
10
ng/L
Achieve levels of detection never seen before in ICP-MS.
100 Above, a typical calibration for 194Pt in chloroplatinin acid
in the ng/L to sub-ng/L range used in the determination of
10
pharmacologically active Pt tracers of anti-cancer drugs.
pg/L
Setting the Benchmark for Ease-Of-Use
Bruker Quantum software

Bruker redefines ease-of-use with our The dynamic Instrument Status window provides a quick
Web-integrated ICP-MS worksheet visual check of the status of all system components. It
software. Quantum features a range is an excellent diagnostic tool that maximizes instrument
up time.
of automated options, including setup
and initialization routines, such as
plasma alignment, mass calibration
and resolution tests. Bruker’s AutoMax
makes method development easy by
automating all ion optics, nebulizer and
plasma settings for optimum results.
Including auto-optimization for Aerosol
Dilution, Bruker Quantum makes light
work of your most difficult samples.
Each worksheet cell

provides all the results
you need – including
concentrations,
intensities, statistics,
replicate readings and
graphical mass scans.
Bruker Quantum switches automatically between multiple method condition sets

within a single sample, giving optimum performance for specific element suites,
without having to re-run samples.
Chemical Analysis Solutions
Laboratory gas chromatography systems
The 400 Series consists of two gas chromatographs and an
associated range of analyzers and solutions designed for leading
applications. These systems allow chemists and engineers to employ
standard methods and/or high quality trace sample analysis, in the
petrochemical, agrochemical and environmental industries.
The 450-GC is a highly affordable and powerful analytical instrument
that offers robust operation in an easy-to-use package. The system
gives users a broad choice of injectors, detectors, switching and
sampling valves up to three channels. The high resolution color touch
screen is intuitive and supports local languages. The Bruker 430-GC
offers the same outstanding performance as the 450-GC but in a
compact, single channel package that occupies about half the bench
space of conventional multi-channel GC.
Triple quadrupole mass spectrometer

The Bruker 320-MS GC/MS stands at the forefront of configurable
triple-quadrupole mass spectrometer systems. It offers: femtogram
sensitivity, 10 – 2000 Da mass range, and a wide array of
chromatographic and ionization configurations to uniquely
match your needs - all in less than 72 cm. (28 in.) of linear
bench space! In minutes, the 320-MS can be changed
from EI to CI modes of operation. Easily,
the 320-MS is the most sensitive,
robust, and flexible triple-quadrupole
MS system currently available.
For research use only.

Not for use in diagnostic procedures.
Bruker Daltonik GmbH Bruker Daltonics Inc.

Phone +49 (0)421-2205-0 Phone +1 (978) 663-3660
Fax +49 (0)421-2205-103 Fax +1 (978) 667-5993
impact
Maximum Impact – Definitive Answers
UHR-TOF MS
Redefining Accurate Mass LC-MS/MS
Until now, mass spectrometry technologies have

forced scientists to choose between performance
characteristics for a given application. Often, a
system is designed for qualitative work, sacrificing
quantitation performance in the process.
There is no need to make compromises in mass
spectrometry anymore. The maXis impact™
sets a new technology standard where industry
leading performance values are all simultaneously
available in a single acquisition at full sensitivity.
Powered by a series of patented technology

innovations, the maXis impact simply provides the
very best results without compromise in a cost
effective, benchtop format.
maXis impact: Qualitative and Quantitative results

from a single LC/MS analysis
Sub-ppm mass accuracy and 40,000 full sensitivity resolution
Extreme sensitivity across the entire mass
range from very small molecules to intact proteins
High speed MS/MS capability with full U-HPLC compatibility
Simultaneous analysis of major and trace sample components
Isotopic fidelity for definitive molecular formulae determination
Robust and easy to operate system
And all in an economical and compact design!
Unparalleled Performance Now in a Bench-top Package
Dual Stage
Reflector
Flight Tube
with in-flight focussing for
full sensitivity resolution
High Transmission
CID Cell
Quadrupole
Patented Dual
Ion Funnel
Long Life
ESI Source Flash Detector
The only no-compromise full Refining capabilities across

sensitivity and full resolution the analytical world
UHR-TOF on the market
With it’s unique capabilities, the maXis
A game changing step forwards in TOF impact liberates your work from restriction
technology featuring nine new patents. and compromise. Whether the need is for
The maXis impact is powered by a small or large molecule analysis, quantita-
number of outstanding technology tion or molecular identification, the maXis
innovations including: impact is more than capable of providing the
results needed to make decisions first time,
Unique Full Sensitivity Resolution
every time.
(FSR) technology in all modes
Proven Bruker UHR-TOF Let the maXis impact achieve your goals in:
technology in bench-top format
Forensics, toxicology & doping control
New broadband transmission CID cell
Food & environmental testing
providing ground-breaking sensitivity
Synthetic chemistry support
Fast 4 G sample/sec digitizer enabling
Drug metabolite and impurity identification
data acquisition at up to 50 Hz
and quantitation
Long life FLASH Detector
Metabolomics
Flexible choice of API sources
Intact protein analysis & characterization
including new CaptiveSprayTM and
of biophamaceuticals
solid sample (DIP) and GC inlets
Biomarker discovery & validation
One-Shot Certainty for Qual/Quant Analysis
maXis impact captures the full picture of Ultra fast acquisition speed up to 50 Hz
your sample in one analysis alleviating 1+
386.2552
the need for multiple runs. More than 1+

1+
386.2548
386.2548
4 orders of fundamental dynamic range 1+
386.2553 Massaccuracy
Mass accuracy better
beter than
than 1ppm
1ppm
1+
achieved without artificial compensators 386.2550
1+
Resoluion25k-30k
Resolution 25k-30k
386.2548
allows simultaneous measurement of 1+
386.2549
1+
major and trace sample components in a 1+
386.2552
386.2548
single analysis - even if coeluent. 1+

386.2552
1+
386.2551
1+
387.2569
1+
387.2575
Buspirone MS/MS linearity 1+

387.2574
1+
0.05 to 500 ppb 387.2584
2.0E+07
1+
1+
387.2584 50Hz
387.2582
1+
45Hz
1+
387.2578
40Hz
1.5E+07
y = 41323x + 55197
387.2576 35Hz
Area
1+
2 387.2578
1+ 30Hz
R = 0.9994 387.2578
25Hz
1+
387.2579
1.0E+07 20Hz
15Hz
10Hz
5.0E+06 5Hz
LOD = 0.05 ppb (100 fg 386.0 386.5 387.0 387.5 388.0 388.5 m/z
1Hz
absolute on column)
0.0E+00
0 50 100 150 200 250 300 350 400 450 500 MS of Buspiron acquired at 1Hz up to 50 Hz spectra to disk.
conc [ppb] Resolution and spectral accuracy maintained even at maximum speed
LOD = 0.05 ppb (100 fg absolute on column)
Qualitative and quantitative results from a single auto MS/MS run
Intens.
x10 6 Intens. 8. +MS, 4.99-5.16min #2324-2422
5
1 TIC
[%]
100 4 MS 223.0750
153.1271
4 80
3 60
196.1697
2
40 135.1160
167.1056
185.1172
109.1008
20 100.0754
1
matrix background 81.0694
123.1161 253.1801
0
0
4 5 6 7 8 Time [min] 25 50 75 100 125 150 175 200 225 250 m/z
Intens.
Intens. 8. +MS2(223.0750), 20.0eV, 4.99-5.16min #2325-2425
x104
[%]
6 MS/MS
4
2 hrXIC 5 mDa selectivity 100 56.0498
80
5 Isotopes
3
60
40
126.0110
1
20 223.0745
167.1066
0
0
4 5 6 7 8 Time [min] 25 50 75 100 125 150 175 200 225 250 m/z
Intens.
x104 1. TIC from complex sample (60 pesticides spiked into ginger extract (QuEChERS)).
4 3 hrXIC zoomed hrXIC 2. High resolution extracted ion chromatogram (hrXIC) of m/z 223.0745 with 5mDa
discrimination width (Acetamiprid).
3
3. Magnified LC peak. High speed acquisition rate in MS and MS/MS mode (MS: 100ms,
100 data points MS/MS: 20ms spectra time). 100 data points across this LC peak allows precise quantitation.
2
MS and MS/MS 4. High resolution survey MS with m/z 223.0745 selected as precursor.
1
5. High spectral accuracy means isotopic fidelity for identification certainty.
6. High sensitivity MS/MS spectrum even at very low MW (56.0498 m/z) for confirmation
0
4.95 5.00 5.05 5.10 5.15 5.20 5.25 5.30 Time [min]
by fragment information.
Precisely Targeted Small Molecule Analysis
Ideally suited for broad band Multi-target screening at 2mDa hrXIC selectivity
multi-target screening Intens.
x105
Mix200_50ppb_GB3_05_997.d
Resolve and identify multiple compounds Amitrole, [M+H]+, m/z 85

2.5
from extremely complex mixtures Avermecin,

across a wide mass range even at low [M+NH4]+, m/z 890
Oxamyl,
analyte concentrations. With these 2.0
Fragment m/z 72 Spinosad A,

key performance attributes, the maXis [M+H]+, m/z 732
impact is an ideal fit for rapid, high target 1.5
number screening tasks such as food

testing or forensic analysis.
1.0
For the laboratory challenged with rapid

response to novel analytes, retrospective
in-silico screening of complete datasets 0.5
using TargetAnalysisTM software makes

maXis impact the most agile screening 0.0
0 2 4 6 8 10 12 Time [min]
tool available.
EICresolution
2 mDa high width 2 mDa
XIC from 200 pesticides. Each color represents the hrXIC
of one pesticide. Sample at a concentration of 50 ppb each separated with
U- HPLC maXis impact.
Triple quad sensitivity and quantitation with high-res, accurate mass The maXis impact encapsulates the
capabilities of low fg range sensitivity
with more than four orders of magnitude
dynamic range and quantitative capacity
along with the performance of an
4 orders of magnitude exceptional high resolution accurate
mass instrument. The simultaneous
combination of attributes is ideal for
many discovery, development, screening
and drug metabolism applications which
often require both the identification and
quantitation of sample components
that vary widely in both structure and
concentration.
Very low LOD Excellent for use in the analysis of both
known and unknown compounds, the
maXis impact represents a new level of
Intens.
2
achievement in the challenge to provide

50 fg
150
one step qualitative and quantitative data

100
in a single rapid analysis.
50
0
9.00 9.25 9.50 9.75 10.00 10.25 10.50 10.75 11.00 Time [min]
Fluoxastrobin (m/z 459) has been quantified over 4 orders of magnitude in

concentration from 1ng down to 50fg. The linearity is R=0.9996 with an ultra low
LOD coupled with accurate mass certainty.
Small Molecule ID & Structure Elucidation
Impacting Metabolomics results Compound identification and structure confirmation
A key bottleneck in metabolomics
research is structural confirmation and
+MS2(949.4275)
elucidation of metabolites. Diterpene O O
HO O
glycosides (DTGs) are abundant plant

1.00 HO
O
O
defence compounds with largely 1+

HO O
OH
unknown modes of action. Outstanding 271.2422

MS and MS/MS data for Nicotiana
0.75 1+
O
attenuata (tobacco) DTGs acquired HO

OH
1+
O
on a maXis impact enabled the rapid 683.3635

identification of the entire compound 395.1181 OH O
family. To achieve this, the fragmentation

0.50 O O
1+
949.4278
O
O
results are visualized and interpreted HO

OH
using Bruker´s novel FragmentExplorerTM. 1+

0.25 537.3057
The FragmentExplorer has been
especially designed for faster inter-
pretation of MS/MS data. 0.00
200 300 400 500 600 700 800 900 m/z
It provides an interactive link between
SmartFormula 3DTM results, mass
spectra and molecular structures.
SmartFormula3DTM co-developed with Pfizer,

utilizes maXis impact high isotopic fidelity
in MS and MS/MS in a unique relational
algorythm to assign molecular formulae of
SmartFormula3D
unknown analytes without prior knowledge or
assumptions.
Intens.
x105
1.2
Fragment Explorer
C 15 H 23 O 12 , 0.32 mDa,
1.0
C 20 H 31 , -0.2 mDa, C 35 H 55 O 13 , 0.27 mDa,

C 24 H 35 O 20 , 0.33 mDa,
C 44 H 69 O 22 , 0.81 mDa,
C 26 H 41 O 4 , 0.1 mDa,
0.8
271.2420
C 15 H 21 O 11 , 0.061 mDa,
C 15 H 19 O 10 , 0.25 mDa,
C 15 H 25 O 13 , 0.35 mDa,
C 18 H 23 O 15 , 0.37 mDa,
C 18 H 25 O 16 , 0.41 mDa,
C 24 H 31 O 18 , 0.48 mDa,
C 24 H 33 O 19 , 0.45 mDa,
C 24 H 37 O 21 , 0.25 mDa,
C 24 H 39 O 22 , 0.38 mDa,
C 29 H 43 O 8 , 0.31 mDa,
C 29 H 45 O 9 , 0.16 mDa,
0.6
683.3637
395.1184
0.4
417.2999
949.4275
C 20 H 33 O 1 , -0.15 mDa,
C 26 H 39 O 3 , 0.13 mDa,
643.1716
0.2
661.1822
537.3058
359.0973 497.1137 625.1611

519.2952
479.1031 607.1505
0.0
300 400 500 600 700 800 900 m/z
FragmentExplorer with embedded ChemDraw expertly assists

fragment assignments.
Biomarker Discovery and Validation
In-depth qualitative analysis of complex proteomics samples
Get full performance

ID more than 1500 first time, every time
proteins with FDR <1%
The CaptiveSpray is a revolutionary
LC-MS source that combines the
sensitivity of nanospray with the ease
of use and robustness of electrospray.
CaptiveSpray utilizes patented techno-
logy to capture and sweep sample ions
into the MS independent of LC flow rate.
LC auto MS/MS survey. The human cell line HT29 is an established model
system to study colon cancer progression. A 1 µg digest of HT29 cells was
separated on a RSLCnano system (3 h gradient, 25 cm column) and measured
on a maXis impact with a captive spray source.
CaptiveSpray LC-MS source
Identification over a dynamic range of 4 decades of concentration from complex samples Real-life MS mass accuracy
4 decades of concentration
1.1 pmol
110 fmol
11 fmol
1.1 fmol
Plot of all peptides from a digest of

200ng E.coli
Sigma UPS-2 standard sample
BioPharma QC
Isotopic resolution of intact proteins

Quantum leap in data
quality for biopharmaceutical
Intens.
characterization
+MS, 4.2-7.5min, Deconvoluted (MaxEnt)
x105 1+
'18265.39460 MS spectrum MaxEnt
1Beta lactoglobulin B, With 30k-40k resolution for intact
5
(1pmol/µl infusion) -deconvoluted of Beta
4
lactoglobulin B (Infusion
proteins @ full sensitivity with
3 of 1pmol/µl) with fully true isotopic patterns, the maXis
2 resolved isotopes impact allows you to simultaneously
1
characterize, impurity detect and
0
quantify your biopharmaceutical
x105 C817H1311N206O248S9, M+nH ,18265.39
Simulated spectrum with a

products. Combined with MS&
5
2 MS/MS accuracy < 1ppm the maxis
4
32,000 Resoluion resolution 32,000
impact ensures certainty first time
3
every time, allowing you to report
2
your results with confidence.
1
0
18265.0 18267.5 18270.0 18272.5 18275.0 18277.5 18280.0 18282.5 18285.0 18287.5 m/z
Source Options
Wide choice of ionization and coupling techniques for
a broad range of sampling including insoluble compounds: GC-MS
coupling
GC-MS coupling
APCI II (atmospheric pressure chemical ionization) source
with direct probe
APPI II (atmospheric pressure photo ionization) source Solid probe
CaptiveSpray LC-MS source mounted on an
CE-MS coupling with grounded needle APCI II source
LC Options
to change specifications without notice. © BDAL 06-2011, #276708
Bruker fully supports and integrates a wide range of leading
HPLC systems, autosamplers and accessories.
APPI II source

Bremen · Germany Billerica, MA · USA Fremont, CA · USA
Phone +49 (0)421-2205-0 Phone +1 (978) 663-3660 Phone +1 (510) 683-4300
Fax +49 (0)421-2205-103 Fax +1 (978) 667-5993 Fax +1 (510) 490-6586
sales@bdal.de ms-sales@bdal.com ms-sales@bdal.com
www.bruker.com/maXis
PesticideScreener™
A complete multi-residue solution for pesticide
screening using high resolution full scan
accurate mass data
LC-MS
The Challenge of Ever Increasing
Number of Targets
Pesticides have become a ubiquitous element in our food
production cycle. As a consequence, their residues in
consumers products must be suitably controlled. Accurate
and reliable screening for the presence of pesticides in food
is therefore a critical analytical task.
However, this task is far from simple. The increasing

number of target compounds with ever-lower detection
thresholds poses special analytical challenges, with
hundreds of different pesticide targets assessed during the
analysis of each food sample coming from a vast variety of
different food products.
A Ready-To-Go Solution
in Your Hands
Bruker’s PesticideScreener delivers a com- Identification of compounds is based
prehensive screening solution for pesticides on combined scoring of mass accuracy,
and plant metabolites in food. Market-lead- isotopic patterns and retention times and,
ing Q-TOF mass spectrometry technology optionally, MS/MS qualifier ion fragment
is combined with a high-quality compound data. The powerful package enables
database and a rigorously tested method, confident and comprehensive multi-
ready developed using SANCO guidelines. target analysis of pesticides in food in a
single LC-MS run.
Our Workflow Solution
Sample Pesticides
QuECHERS extract food, feed, water
LC-QTOF-MS
U-HPLC + maXis impact
Sub-ppb sensitivity Multi-target screening application s/w
Full sensivity resolution
1-2 mDa hrXIC
true isotope pattern
One shot qual/quant analysis

with no method development
Processing: In-silico
TargetAnalysis retrospective
High Quality analysis
Pesticide Database of new targets
Identification
Confirmation
Quantitation
Reporting
maXis impact delivering wide dynamic range for quantitation of
screening results: example calibration curve for methomyl
Complete solution consisting of U-HPLC & QTOF hardware, applications software, ultra-stable LC-MS methods for
the entire workflow. Plus high-quality multi-target screening database.
Automation & High-Quality
Database
Ready-to-go, no method development required
• At the heart of Bruker’s Pesticide

Screener is a high-quality data-
base of more than 700 target
pesticides.
• Far more than a “list of
compounds”
• Retention times for the matched
UHPLC method
• Adduct information
• Isomer information
• Fragment ions on MS data level
• Isotopic confirmation
2
• Qualifier ions for confirmation in
broad band MS/MS mode
• For definitive analyte detection
Processing is completely automated with TargetAnalysis
Results Overview • Data are screened and scored

Level of confidence against the database information
for at-a-glance confidence
• Use qualifier ions (and their
intensity ratios) from broad band
MS/MS for enhanced confirma-
tion (++++)
3
• Enables fast and reliable identi-
fication of target compounds at
ppb levels, with very low false
Detailed report on bbCID qualifier ions positive rates even in complex
matrices
• Get quantitative results via
integrated link to quantitation
software
• Perform in-silico retrospective
analysis of new targets at a click
of a mouse
High Precision Data Means Ultimate
Selectivity
hrXIC = High-resolution extracted ion chromatogram / XIC of the mass +/- 2 mDa
Intens.
x106
2.5
2.0
BPC
1.5
ragm 165
Linuro
1.0
Methoxyf e
Imazalil
Carbaryl Phosmet Fludiox
Bos
0.5
Azinphos-methyl Fragm 1
Meth
Dimetho
Azinphos-methyl Fragm 1
DimethomorphChlo
0.0
1 2
4
3
5
7
8
6
91112
10 15
14
16
13 17 1820
19
22 23
21 24
25
28
29
26
27
31
30
34
32
33
35 36
37
42
38
39
4044
41
4345
49
46 474850
51 55 5859
54 56
5253 57 64
62
6061
63 65
66 68
6774
71
73
70
72
69 75
77 78
76
7982
80
88
83 86
81 85
84 90 92
8789 91 93 95
96 101
98
102104
94 97100
99
103105
107
1
106
108 09
110 111
112
115
113
114
Chlorantraniliprole
2 4 6 8 10 12 14 Time [min]
Intens.
Azoxystrobin(Guthio
Azinphos-methyl Fragm
x104 Carbaryl Fragm 145
arbaryl (NH4) Phosmet (NH4)
8
Flud
hrXIC 8
Aldicarb Fragm 116
Methamidophos Pyriproxyf en
4
Trif loxystrobin
2 Etof enprox (NH4)
Amitrole
Cyprodinil
Propargite (NH4)
Diazinon
Pyraclostrobin
Carbendazim Ethion
Phosmet Fragm 160
Methomyl Fragm 88 Pirimicarb
Oxamyl Fragm 72 Carbof uran Metalaxyl Pyrimethanil
Methomyl
OxamylMonocrotophos
Fragm 90 Thiabendazole
Fragm 127
2
Azoxystrobin Quinoxyphen
Acetamiprid
Thiacloprid
Triazophos Spinosad AIndoxacarb
(Spinosyn A)
Monocrotophos Dimethoate Carbof uran Fragm 165 Bif enthrin (NH4)
Methomyl Fragm 106 Linuron Kresoxim-methyl
Tebuf enozide Fragm 297
Prochloraz Cyhalothrin. lambda- (NH4)
Imidacloprid Butocarboxim Fragm89
75 Penconazole Prof enophos
Omethoate Monocrotophos Fragm 98 Aldicarb Fragm Methoxyf enozide Fragm 313
Imazalil
Tebuconazole
Carbaryl Phosmet Dif enoconazole Peak 21
Fludioxonil (NH4)
Boscalid Propargite Fragm 231
Chlorpyriphos
Azinphos-methyl Fragm 132 enozide EPN
Methoxyf
Dimethomorph Peak 2
Monocrotophos Fragm 193
Carbendazim Azinphos-methyl Fragm 160
Dimethoate FragmFragm
199 160 Tolyf luanid
Tolyf luanid
Kresoxim-methyl Fragm
Fragm Indoxacarb
137
206 (Na)
Dimethomorph Peak 1 Fenhexamid
Chlorpropham FragmTebuf
Myclobutanil
Triadimenol
172 enozide
II Fragm
Spinosad A (Spinosyn A) Fragm 544
70 luanid (NH4)
Ethion Fragm 199 Etof enprox Fragm 359
Tolyf Pendimethalin
Chlorantraniliprole Cypermethrin II (NH4)
Oxamyl (NH4) Azoxystrobin(Guthion)
Fragm 372 Fragm 238FragmSpinosad
Tolyf luanidProchloraz 308
D Pendimethalin Fragm
Cyhalothrin. 212
AvermectinB1a
Cypermethrin
lambda- (Abamectin) (NH4)
III (NH4)
(Na)
Azinphos-methyl Triadimenol II Chlorpyriphos-methyl
183Monocrotophos (NH4)
Omethoate FragmOxamyl Aldicarb (NH4) Carbaryl (NH4) Phosmet (NH4) Triadimenol
Chlorpropham
I FragmKresoxim-methyl
70Spinosyn B or (NH4) Spinosad D (Fragm 558)
K (last of 3-4 peaks) Cypermethrin
Propargite II (Na)
AvermectinB1a
Cypermethrin III (Abamectin) (Na)
Butocarboxim Fludioxonil
Triadimenol II (Na) II (Na)
0
2 4 6 8 10 12 14 Time [min]
Intens. 41. +MS, 8.83-9.05min #523-536, found: Phosmet (C 11 H 12 N 1 O 4 P 1 S 2 , 316.9945, [ [M+H]+: 318.0018])
x106
373.1302
1.25
1.00
phosmet,
0.75 experimental,
0.50 full scan
0.25
100.0755 622.0290 767.2326
0.00
41. Phosmet(C H N O₄
P S , 316.9945, err[mDa]: -0.15, mSigma: 9.1)
8000 318.0018
6000
4000
theoretical
2000
0
100 200 300 400 500 600 700 800 900 m/z
Intens. 41. +MS, 8.83-9.05min #523-536, found: Phosmet (C 11 H 12 N 1 O 4 P 1 S 2 , 316.9945, [ [M+H]+: 318.0018])
8000 318.0020
6000
4000
2000
317.0917 319.0046
319.9987
321.2063
0
41. Phosmet(C H N O₄
P S , 316.9945, err[mDa]: -0.15, mSigma: 9.1)
8000 318.0018
6000
ID by mass
accuracy/isotope
4000
pattern
2000
319.0046
319.9991
0
317.0 317.5 318.0 318.5 319.0 319.5 320.0 320.5 321.0 m/z
High-resolution extracted ion chromatograms are used to pull components out of complex matrix backgrounds.
Interrogating the compound database, accurate-mass and isotopic fits are calculated and checked automatically,
along with retention times and, where necessary, fragment ions. This combination of data definitively confirms the
presence of target pesticides.
Result confirmation by parallel detection
of diagnostic ions
The impact of knowing compound behavior
Proof of identity, detection of adducts and fragments for unambiguous identifications
Intens.
x104 8
CVUA_2-5_1ppm_RA1_01_1072.d same sum formula,
8 almost coeluting,
Aldicarb no M + H detectable
Butocarboxim:
6 m/z 75: C3H7S
Butocarboxim
[M+NH4]
2
9
5
46 7
0 Aldicarb:
5.8 5.9 6.0 6.1 6.2 Time [min] m/z 89: C4H9S
Only a screening database with knowledge about fragmentation behaviour yields to correct identifications.
Reconfirmation: detection of adducts and fragments for enhanced confidence in findings
Intens.
CVUA_2-5_1ppm_RA1_01_1072.d
x10 5 Methiocarb Fragm 169
31
2.0
Halofenozide Fragm 275

27
Promecarb Fragm 151
37
1.5
Fenobucarb
21
1.0 Methiocarb/Mercaptodimethur
30 Promecarb
36
Fenobucarb Fragm 152 Halofenozide Fragm 105
28 Promecarb Fragm 109
22 35
Fenobucarb Fragm 95
20
0.5 Halofenozide (Na)
Fenobucarb Fragm 151 25
23 Halofenozide
26 Fludioxonil Fragm 229
Fludioxonil (NH4)
Methiocarb (NH4) 32
34
Fenobucarb (NH4) 29
19 Fludioxonil
0.0 33
8.8 8.9 9.0 9.1 9.2 9.3 9.4 9.5 9.6 9.7 Time [min]
In this example, the identification of Fenobucarb is re-confirmed by the presence of 4 diagnostic ions (3 fragment ions, NH4-adduct).
Why Use bbCID Fragment Ions ?
What is bbCID? Alternative acquisition of full scan data with/without fragmentation.

Example of interference in Orange, spiked 10 ppb
• In this spectrum, an interference

Intens. 10ppb_bbCID_Orange_2ul_RB4_01_2853.d
x106
BPC peak m/z 403 is masking

1.2
1.0
azoxystrobin m/z 404
A zoxystrobin
XIC@ m/z403
44
0.8
9.0 • But, the CID method generates

0.6
fragments in parallel
0.4
• Each individual fragment is

0.2
identified by accurate mass,
isotope pattern and intensity ratio
A zoxystrobin
44
0.0
8.0 8.5 9.0 9.5 10.0 10.5 11.0 11.5 Time [min]
Intens. 44. +MS, found: Azoxystrobin (C 22 H 17 N 3 O 5 , 403.1168, [ [M+H]+: 404.1241])

x10 5
373.1285
1.25 Reference: SANCO/12495/2011:

Method validation and quality
1.00
azoxystrobin, control procedures for pesticide
experimental, residues analysis in food and
0.75
full scan 403.1393 feed: Where an ion chromatogram
0.50
shows evidence of significant
389.1236 chromatographic interference,
0.25
! it must not be relied upon to
345.0970
359.1130 quantify or identify residues.

0.00
0.0
x10 5 44. +bbCID MS, 25.0eV 372.0979 (0.5), 344.1030 (-1.4), : points : 3
• So, azoxystrobin is still
bbCID
373.1285
definitively identified
1.5
403.1395
• The database contains qualifier
ions for species which may require
1.0
extra information
0.5
344.1016 (-1.4), %I 0.23 372.0984 (0.5), %I 1.03
395.1112
• m/z 344.1016 and m/z 372.0984 are
389.1237
qualifier ions. Brackets represent
358.1051
345.0975
relative intensity range.
Highlest level of confidence:

• ID by mass accuracty, isotopic pattern and adduct information
• Confirmation by diagnostic ions and bbCID
• Very low false positive rates
maXis impact: Highest Performance
Benchtop Q-TOF
One, no compromise mode:

• Resolution and speed simultaneously
for coupling to high-res LC
• Full Sensitivity Resolution (FSR)
• One shot method
• Excellent sensitivity, high dynamic
range
• True Isotope Pattern (TIP): For ultimate
confidence in the molecular formula
• Extracted ion chromatograms (hrXIC)
+/- 1 mDa
Resolution Maximum Speed Accuracy/ppm True Isotope Pattern

(FWHM) (spectra/second) confidence
MS and MS/MS
maXis impact 40,000 50 0.8


PesticideScreener Solution:
• Ultimate robustness of LC methods
• System-to-system reproducibility <0.1 min RT
• Ultimate confidence in results
• Ready-to-go with methods and high quality database to change specifications without notice. © BDAL 09-2012, #703162

Bremen · Germany Billerica, MA · USA Fremont, CA · USA
Phone +49 (0)421-2205-0 Phone +1 (978) 663-3660 Phone +1 (510) 683-4300
Fax +49 (0)421-2205-103 Fax +1 (978) 667-5993 Fax +1 (510) 490-6586
www.bruker.com
Dedicated to the Most
Challenging Applications
solariX, the next-generation line of
hybrid Qq-FTMS systems, is the culmination of
key technology enhancements that provide unique
capabilities in mass spectral
performance and versatility.
The technological advances

encompassed by solariX provide:
Superior Sensitivity
Unmatched Mass Accuracy and Broadband
Resolution
Widest range of structural tools, including
Electron Transfer Dissociation (ETD)
Expansive Mass Range
Selective Ion Enrichment and Enhanced
Dynamic Range
Application Directed and Optimized Solution
Packages
Common applications for solariX

The analytical power and performance of FTMS
is well suited to address some of today’s most
challenging and complex samples. Drawing
on years of applications experience, we have
combined our unique FTMS instrumentation and
comprehensive software tools to provide turn-
key solutions for the following areas:
High End Proteomics Studies

(Top-down and Bottom-up workflows)
Molecular Imaging of Tissue - Distribution of
Drugs, Metabolites, and Biomarkers
Petroleum Product Analysis
Complex Environmental sample analysis
Metabolomics Research
Performance Beyond Compare
At the core of the solariX is dramatically

Base Peak Chromatogram for LC-MS and LC-MS/MS of
improved sensitivity and dynamic range. protein mixture
This allows researchers to identify and
analyze a much wider range of molecules
than ever before, and to delve deeper
into complex mixtures and analyze lower
abundance species.
More power for greater

complexity
The broadband, ultra high resolving
power (increased 8-fold) and superb
mass accuracy of solariX is more
powerful than previously possible with
any other mass spectrometer. This
extraordinary increase in the number of
available m/z channels is essential for
addressing complex problems such as
petroleomics and environmental samples
which require resolving powers of greater
than 400,000 for effective analysis.
Effective analysis of complex problems
Faster and more advanced LC-MS
and LC-MS/MS operation Crude Oil, APPI positve
Intens.
Intens.
x108
303.21414
x109
The new data acquisition functionality

5
enables smarter modes of data
dependent operation. Here, spectral 303.21078
1.5
acquisition parameters such as data set 4
size or starting mass may be adjusted

on-the-fly in a mass dependent mode 3
303.20967
of operation. Smaller data sets can 1.0
303.20630
303.19815
be selected for MS/MS acquisitions 2
making the overall data acquisition rate

faster, while maintaining the high fidelity 1
303.19366
measurements for the MS precursor 0.5
acquisitions. Moreover, super stable mass 0
303.195 303.215 m/z
accuracy is maintained throughout the

LC-MS analysis using Bruker’s proprietary
Ion Charge Control (ICC™) mode. 0.0
200 400 600 800 1000 1200 m/z
Broad band spectrum of a crude oil measured by APPI in

positive ion mode. Insets illustrate the extreme
high resolving power (> 550,000) of the solariX-CM.
New Horizons for Biomolecule Analysis
Expanded capabilities for
Myoglobin, non-denaturing MS conditions
biomolecule analysis
Resolving Power = 450,000 @ m/z 1953
Adding to the existing arsenal of
structural fragmentation tools, solariX
is fully enabled with Electron Transfer
Dissociation (ETD). This exciting new 100
technique is superb for in depth,
comprehensive analysis of proteins and 80
peptides and their subtle, posttranslational
Relative Intensity
modifications. For instance, the gentle 60
molecular dissociation chemistry
associated with ETD enables researchers
40
to elucidate subtle post-translational 1952.0 1952.5 1953.0 1953.5 1954.0 m/z
modifications such as glycosylation and

20
phosphorylation at levels of accuracy and
resolution previously unavailable for such
0
de-novo approaches. Furthermore, ETD 1950 2000 2050 2100 2150 2200 m/z
can be automated, and combined with

LC-MS/MS schemes in combination with
quadrupole fragmentation (Q-CID).
ETD + ECD of Calmodulin
Abs. Int. * 10e 6

c EAF S K DGDGT I TT K E T R Q N E
ECD
c+1 E
z+1 E K* EG HR A GN D K
z+2 K
1562.771 3260.548
c 13 (781.89 2+) 1909.918 2615.252 c 29 (1630.78 2+) 3788.841
350 c 34 (1263.62 3+)
c 16 c 22 (1308.13 2+)
2844.324
300 1068.499 3159.504
c 25 (1422.67 2+) 3687.798
z+1 9 1691.814 2372.125 c 28 (1580.26 2+)
c 14 c 20 (1186.57 2+) c 33 (1229.94 3+)
250 2630.157
939.457 3058.444
z+1 23 (1315.58 2+) 4719.156
z+1 8 1475.649 1996.949 c 27 (1529.73 2+) 3475.511
200
150
z+2 12
Abs. Int. * 10e
c
c 17
9
S
2672.274
DG(1336.64
c 23 T TK 2+) Q2945.372 A N
3388.647
z+1 30 (1738.26 2+)
K DTD
3804.671
z+1 41 (1573.72 3+)
E E K N I R
ETD
1359.634 z+1 1762.853 E c 26 (1473.19
H R 2+)
A NGD K D F V R E TD K M K
100 z 11 c 15 c 30 (1694.83 2+) 6887.161 z+1 33 (1902.84 2+)
2.5 2672.230 4560.176 6501.970 7622.467 8297.729 8225.732
2500.228 5071.396 5816.598 c 63
c 232+) 2787.308
c 41 c 59 z+1 66 z+1 72 c 74
c 21 (1250.62 c-1 46 3003.329z+1 51 3686.790
50 1701.767 c 24 (1394.16 2+)
z+1 14 3260.470 z+1 26 (1502.17 2+) c-1 33 (1843.90 2+)
6102.713
2.0 2615.212 4432.121 6591.904 7493.441 8169.608 8945.079
0 c 29 5254.344 z+1 54
c 22 c 40 z+1 58 z+1 65 z+1 71 c+1 80
z+1 45
2000 4000 6000 8000 10000 m/z
3217.465 5930.621 6230.777 7095.181 8556.803
2500.184 z+1 28 4387.911 5012.237 6492.839 8054.605
1.5 z+1 52 z+1 55 z+1 62 z+1 74
c 21 z+1 38 z+1 43 z+1 57 z+1 70
3159.425 5442.571 6967.171
1996.924 3686.728 4856.107 5987.628 6329.920 7953.541 8428.743
c 28 c 49 z+2 61
1.0 c 17 c-1 33 z+1 42 z+1 53 c 57 z+1 69 z+1 73
2945.307 5483.448 6616.002

1909.894 3388.592 4719.034 5915.754 6345.795 7839.478 8070.620
0.5 c 16 N-Acetylalanine
c 26
c 30 z+1 41
z+1 48 c 53 z+1 56 c 60
Trimethyllysine
z+2 68 c+1 73
2844.266 5325.373 6748.061

1562.753 3346.420 4718.084 5931.625 6258.887 7375.339 8298.717 8728.009
c 25 z+1 46 z+1 59
c 13 z+1 29 z 41 z+2 52 c 56 c 67 z+2 72 c 78
0.0
2000 4000 6000 8000 10000 m /z
1 148
Phosphothreonine Phosphotyrosine
The combination of ECD and ETD performed on Calmodulin. As illustrated above, the expected phosphorylations
are not present and the lysine at position 116 is trimethylated.
Definitive molecular identification
With the solariX, high sensitivity, superior
quality, exact mass measurements are
only a mouse click away. Leveraging
sub-ppm levels of mass accuracy for both
intact precursor (MS) and product ions
(MS/MS) combined with accurate isotope
patterns, SmartFormula3D™ provides
definitive elemental composition and
molecular formula information. This level
of confidence is readily achieved without
internal standards or recalibration and the
high resolution data inherently mitigates
the complications resulting from other
chemical interferences.
Molecular formulae determination of components in crude oil
High resolution spectrum of crude oil by APPI demonstrating

broadband resolving power of 950,000 with average mass
measurement error below 100 ppb.
325.19510 325.19845
C25H25+ C22H29S+
-0.1 ppm 0.0 ppm
325.19399
13C C H S+•
1 21 28
0.0 ppm
325.19064
13C C H +•
1 24 24
0.1 ppm
325.18253
C24H23N+•
0.1 ppm
325.20178
325.17805 325.21617
13C C H N+ C19H33S2+ C22H29O2+
1 23 22
0.1 ppm 0.1 ppm
0.1 ppm
325.180 325.190 325.200 325.210 m/z

CASI™: For rapid, enhanced
CASI Figure
selectivity and dynamic range
The unique Qq-FTMS geometry of
the solariX can enrich lower abundant
or trace species for detection and
structural interrogation via MS and MS/
MS, respectively. This mode, known as
Continuous Accumulation of Selected
Ions (CASI), is essential for tissue
imaging, analyzing low-copy PTMs, and
to extend the general dynamic range
of almost any measurement. This can
improve signal intensities by as much as
an order of magnitude.
“Q”
Schematic of CASI workflow where target ions

are selected and enriched in the collision cell.
Solution packages optimized for a

variety of applications
The solariX product line features a series
of tailored solutions around specific
applications. Each solution comprises
the appropriate hardware, ion source
options, and application level software for
a turn-key analytical platform. Whether
your primary application is complex
mixtures (e.g. petroleomics) or high
resolution tissue imaging, a solariX
package is designed to meet your needs.
For laboratories having unique or multiple
analysis needs, Bruker will work with you
to prepare an appropriate, customized
configuration.
High-End Performance for any Analytical Laboratory
m/z = 313.1485
CASI boosts
sensitivity, enabling
the detection of drug
and metabolites at
“q” lower concentrations
Full mass range MS CASI - 20 m/z window
Molecular imaging of olanzapine in kidneyDosage – 5 mg/kg, 6h post dosage analysis.
The solariX FTMS is as intuitive and

easy to use as a benchtop instrument.
Bruker’s Compass™ software enables
the researcher to harness the analytical
power and versatility with ease so that
the focus is on the application and not on
the instrumentation.
Weekly cryogen “fills” become

annual…
Bruker’s patented refrigerated magnet
technology means nitrogen-free compact
superconducting magnets with very low
helium losses to minimize instrument
maintenance and service. Patented active
shielding technology minimizes the
stray magnetic field levels for compact
installations and maximum laboratory
safety. These magnets are available for
the full range of magnetic field strengths
(7T, 9.4T, 12T, and 15T).
Unique Features of solariX
Ultimate versatility in structural

analysis
The solariX leverages the complementary
nature of traditional quadrupole based
Collisional Induced Dissociation (CID), and
now Electron Transfer Dissociation (ETD).
Additionally, precursors of complex
mixtures may be isolated and fragmented
using high front-end resolution, in-cell
isolation followed by Electron Capture
Dissociation (ECD) or Sustained Off-
Resonance Irradiation (SORI)-CID.
Whether your application is natural
product, peptide/protein, carbohydrate,
orpetroleum product analysis, solariX has
an array of fragmentation tools to address
practically any compound class.
Ion source flexibility

While FTMS is ideally suited to liquid
introduction, Bruker Daltonics offers the
unique dual ESI/MALDI source. This is
based on patented Ion Funnel technology
for maximum sensitivity which allows
effortless switching between ESI and
MALDI – all at the touch of a button! The
intermediate pressure MALDI source
offers exquisite sensitivity, and preserves
molecular ion fidelity throughout the
complete FTMS detection process. The
efficiency of the dual ESI/MALDI source
combined with the new ion optics of the
solariX provides a marriage of ultra-high
resolution with high-end “MALDI-TOF
sensitivity”.
Along with our array of atmospheric
pressure ionization sources (ESI,
nano-ESI, APCI, and APPI), Bruker
now offers GC-APCI capability.
Flexible Front-end Solutions
Compass
The powerful Compass software enables the operation of a range of

industry standard HPLC and u-HPLC platforms with the solariX.
This includes comprehensive system control and seamless integration
of mass spectral and UV data.
solariXcontrol™ - the instrument control software for solariX

Magnet 7.0T/US/R refrigerated magnet
Field strength 7.0T

Analytical Performance
Mass Range 100 – 10,000 m/z (transmission with RF only)

100 – 6,000 m/z (mass selective)
MS/MS Operation
Isolation efficiency (Qh-Interface)

> 60%
(LHRH, [M+2H]2+)
MS/MS Efficiency (Qh-Interface) > 50% conversion from isolated precursor

(LHRH, [M+2H]2+)
Multistage MS (MS3 guaranteed) LHRH MS/MS (collision cell) ->

(LHRH) MS/MS/MS (Infinity Cell™)
Automated isolation and MS/MS of the most

Mass Dependent MS/MS intense ions in an LC-MS/MS run (scan ratio
set to five)
Sensitivity ECD S/N > 10:1 for 5 fmol (consumed).

(Substance P) c5 fragment @ m/z 624
ESI
Mass accuracy <1.0 ppm, m/z range 100 - 1500 (internal)

(Calibration on any 8 Q-CAD fragments <1.5 ppm, m/z range 100 – 1500 (external)
for Angiotensin 1. checked on 4 different *Spec is based on average of errors
masses)
Resolving Power @ m/z 400 (lincomycin) > 1,000,000 (FWHH)

Sensitivity
S/N > 10:1 for <100 amol (consumed)
(Ubiquitin)
High mass
S/N > 10:1
(BSA 0,1mg/ml)
Negative ions Functionality shown on Fibrinopeptide B
ESI Source Options
Electrospray (standard) (1 µl/min – 1 ml/min)
On-Line Nanospray (standard) (100 nl/min – 500 nl/min)
EZ Nanospray (standard) Zero – Adjust Off line Nanospray
APCI (optional) Optional accessory

APPI (optional) Optional accessory
Off-Line Nanospray (optional) Optional accessory

For research use only. Bremen · Germany Billerica, MA · USA Fremont, CA · USA
Not for use in diagnostic Phone +49 (0)421-2205-0 Phone +1 (978) 663-3660 Phone +1 (510) 683-4300
procedures. Fax +49 (0)421-2205-103 Fax +1 (978) 667-5993 Fax +1 (510) 490-6586
www.bruker.com
Best-In-Class Ion Trap Mass
Spectrometer for Routine Analysis
The amaZon SL entry-level system is based on Bruker‘s advanced amaZon ion trap series and
delivers best-in-class analytical performance, enhanced productivity and efficiency – all tailored to
your needs. Together with high sensitivity – thanks to proprietary Dual Ion Funnel technology – and
fast data acquisition at high scan speeds and mass resolution, this ion trap delivers uncompromised
LC/MSn data quality and flexibility at an affordable price.
Bringing innovative trap

technology within the reach
of every analytical lab
The amaZon SL sets a new standard in ion

trap excellence, making high-quality and
high-confidence chemical analysis affordable
for any analytical lab.
By enabling rapid and sensitive identification,

characterization and structural confirmation
of compounds, the amaZon SL is the robust,
all-purpose solution to a wide variety of
chemical, environmental, metabolic or
forensic challenges:
General LC/MS/MS chemical analysis
Chemical structure confirmation by MSn
Screening applications with fast polarity
switching and MS/MS library searching
Quality control, synthesis control and
process development
Metabolite ID & profiling
Copolymer analysis by
GPC-MS/MS
Ease of Use
Robustness
Intens. 150
[%] MS 3+ MS
349.52 100
MS and MS/MS spectra of a mixture of
mass position / mDa

100
50
80 3+
peptides. The enhanced resolution scan 60 433.23 2+ 5+
2+ 0
810.42
524.28
provides fully resolved peaks up to a charge 40 630.69 -50
-100
state of 4+. The observed mass accuracy 20
0 -150
for the precursor ion at m/z 810 and the 200 300 400 500 600 700 800 900 m/z
150
2+ 3+ 4+ MS/MS 810.42
fragment ion at m/z 736 is absolutely unri- 810.42 587.32 324.93 736.41 MS/MS 100
mass possition/mDa
50
valled for ion trap instruments. A continuous
0
AutoMSn experiment over a period of 24 h -50
demonstrate the excellent robustness of 456.27 -100
24 hour continuous AutoMSn
the instrument with regard to an extremely 200 400 600 800 1000 m/z
-150
2 4 6 8 1012141618202224
stable mass position of the detected ions.
Performance and reliability Sensitivity

The amaZon SL stands for excellent Intens.
performance and reliable, robust

6 x 2.5 pg
operation. MS and MSn data sets
deliver high sensitivity, resolution of
multiply charged ions and class-leading, 6 x 1.0 pg
confident mass assignment. 6 x 250 fg
S/N > 50:1
Simple and flexible quality &

synthesis control 10 15 20 25 30 Time [min]
The flexible APCI source also offers

the possibility to work with the direct MS/MS sensitivity for multiple injections of reserpine. S/N ratios > 50:1 are
injection probe (DIP) for the analysis reproducibly obtained using 250 fg column loads.
of solid samples. This enables a fast
and direct identification of compounds
from organic syntheses, process
development, etc.
Intens.
TIC 263.03
MS
N O
O
O
N
246.06
Solid Samples O
246.05
2.50 3.00 3.50 4.00 Time [min] MS 2

The direct probe ion source allows the straight-
forward analysis of liquid and solid samples
without tedious sample preparation. The example 231.04
shows the unambiguous identification of a MS 3 216.06
synthetic compound using MS 3. The information 173.02 188.04
provided by MS2 alone is not sufficient due to 144.00 159.99 203.03
non-specific water loss. Samples were kindly 120 140 160 180 200 220 240 260 m/z
provided by SiChem GmbH, Bremen, Germany.

Compound Screening & ID
MSn for structural elucidation Elucidation of glycan structures

and compound screening
Intens.
Single fragmentation pathways in MSn Pent
steps allow observation of side-chain

Hex Hex Dig 1227.7 MS
losses and enable verification or
even de-novo elucidation of molecular Hex Hex
structures. Compounds can easily be
screened and identified by comparing
results with reference spectra stored
in MSn libraries. 1095.7 MS2
903.6
771.5
609.4
[Hex-H2O-H]-
MS3
161.0
200 400 600 800 1000 1200 1400 m/z

Robustness and ease-of-use make
amaZon SL perfect for automated QC MSn enables characterization of digitonin, a steroid glycoside. The
CompassTM OpenAccess enables non complete glycan structure, down to the initial sugar unit, can be
MS-experts to run samples or batches elucidated in consecutive MSn steps.
using predefined methods
Chemical synthesis, quality

control
The amaZon SL can be used in an
Open-Access environment without
special training. The LC-MS walk-up
solution allows a true multiuser
environment – perfectly adapted to
Intens.
the robustness and ease-of-use of the x10 9
4
DK1622 A-X_3-A,1_01_605.d: BPC 100.0-1000.0 +All MS
amaZon SL. The example above shows 3 BPC positive mode

2
the verification of a chemical‘s presence 1
in a synthesis quality control workflow. 0

8 DK1622 A-X_3-A,1_01_605.d: BPC 100.0-1000.0 -All MS
x10
* *
The user receives the result as a PDF 1.0
0.8
BPC negative mode
report. The color-coded 96-well plate 0.6
0.4
0.2
image provides a quick overview for 0.0
every sample in a full batch.

0 100 200 *300 400 500 600 700 Time [s]
*
Quick and reliable identification of natural compounds from myxobacteria by

library search. Sensitive and highly reproducible MS/MS data allow direct
comparison of experimental data with library reference spectra. Fast polarity
switching delivers detection of compounds in both ionization modes and
ensures that compounds are not missed.
Superb Performance United with
Simplicity and Reliability
Exceptional analytical value Reliability and robustness

Patented Dual Ion Funnel technology The amaZon SL is the latest product
delivers superior sensitivity in the low of over two decades of Bruker ion trap
femtogram range. technology development.
Fast data acquisition with scan speeds Robust, proven ion trap technology
up to 32,000 u/s at full isotopic combined with the latest hardware
resolution provides high duty cycles controllers makes the amaZon SL
ideal for UHPLC coupling. a reliable long-term partner for daily
SmartFrag™ algorithm ensures laboratory work.
optimal, reproducible fragmentation Low maintenance requirements and
for MS/MS library searches. reliable hardware make the amaZon
On-the-fly polarity switching (delay SL ideally suited for open access
<80 ms) for highly efficient compound environments, such as in synthesis
MS/MS screening. control or general QC.
Fragmentation up to the MS11 stage,
enables detailed structural investigation Simple, intuitive operation modes
of compounds.
The SmartLine software suite provides
extremely easy and quick access to
Flexibility in your daily work
analytical answers.
The amaZon SL is fully integrated into Smart and intuitive automation
the Bruker Compass™ software suite, routines for calibration, tuning and
allowing flexible use and full control of data post-processing.
a wide variety of HPLC, UPLC and Simplified GUI.
nanoLC systems. Compass™ OpenAccess enables
The instrument is fully compatible with use of the amaZon SL by multiple,
any Bruker API source, giving access to non-expert, walk-up users.
multiple ionization methods, such as
ESI, APCI, APPI, and nanoESI.
Turning ideas into successful analyses

The outstanding performance of amaZon ion trap mass spectrometers is based on and secured by a series of exclusive,
patented technologies.
Patent Benefit Current amaZon-related patents world-wide
Dual Ion Mass-independent ion transfer and DE 195 23 859 C2; GB 2 302 985 B; US 5,572,035 A; GB 2 402 261 B;
Funnel Source sensitivity boost US 7,064,321 B2
High Transmission
Fast alternating polarity DE 195 15 271 C2; GB 2 300 295 B; US 5,736,740 A
Glass Capillary
Non-linear CA 2 010 234 C; DE 689 13 290 T2;

Ultrafast scan rates with unmatched
Excitation for EP 0 383 961 B1; US 4,975,577 A; DE 41 42 870 C2; GB 2 263 192 B;
mass resolution
Ion Ejection US 5,386,113 A; DE 41 42 869 C1; GB 2 263 191 B; US 5,298,746 A
Smart Charge Optimization of the charge density DE 43 26 549 C1; GB 2 280 781 B; US 5,559,325 A; DE 197 09 086
Control inside the ion trap without the need B4; GB 2 322 961 B; US 5,936,241 A; DE 100 27 545 C1; GB 2 364 821 B;
for a time-consuming pre-scan US 6,600,154 B1
Superior high-capacity trap Neutral loss scan
using the latest Dual Ion Funnel Multiple reaction monitoring
technology (MRM) using MS/MS and MS 3
Manual MSn up to n = 11 in all
Quadrupole ion trap based on
scan modes
innovative technical design for the
AutoMSn tree experiments with up
highest ion storage capacities
to 5 fragment ions per stage
Patented SmartICC™ for optimum ion
accumulation control without prescan
Data-dependent experiments
Mass-independent ion transmission
using Dual Ion Funnel technology All forms of common data-dependent
Standard-setting combination of mass experiments – including preferred mass
resolution, scan speed and m/z range lists for automated feedback experiments
in MS and MSn from MetaboliteTools – are supported.
Fast polarity switching for acquisition
of positive- and negative-ion spectra Source options
under LC conditions Choice of API sources
Reproducible spectra for high-confi- Advion Triversa NanoMate
dence MS/MS library searches using Online/Offline NanoElectrospray
SmartFrag™ Smart CE-MS coupling with
grounded needle
Scan speed and resolution (MS and MSn)
Software options
Scan Mode Res m/z u/sec
MetaboliteToolsTM software for
UltraScan™ 2+ ions 2,200 32,000
metabolite identification
Enhanced ACD Labs MS Manager for structural
4+ ions 2,200 8,100
Resolution interpretation and classification
Extended Compass OA/QC: Web-based,
4,000 27,000
Mass Range guided operation of LC/MS systems
for walk-up users
Scan modes Compass Security Pack™ for
Full scan MS, MS/MS and MSn for the regulated environments
most sensitive and reliable analysis of
unknown compounds
Support of HPLC and sample inlet systems from the following vendors:
Bruker EASY-nLC (I &II), Dionex, Advion TriVersa NanoMate, Agilent, VWR/Hitachi,
Waters (incl. UPLC), Autosamplers from CTC
www.bdal.com Bruker Daltonik GmbH Bruker Daltonics Inc.

Phone +49 (0)421-2205-0 Phone +1 (978) 663-3660
Fax +49 (0)421-2205-103 Fax +1 (978) 667-5993
EVOQ TM
Triple Quadrupole Mass Spectrometer
MASS SPECTROMETRY
A GAME CHANGER CREATES Key Applications

SUCH A DISRUPTIVE ADVANTAGE, IT Food testing requires fast results because of
the perishable nature of food. The EVOQ LC-TQ
delivers the fastest sample-to-report time using
TRANSFORMS THE ACCEPTED RULES PACERTM software.
Environmental analysis can be made cost-
effective by using on-line extraction (OLE) using the
space-saving AdvanceTM UHPLC with OLE.
Toxicology testing covers a wide range of
compounds in dificult matrices, which can be easily
ionized by VIP heated-ESI technology.
Pharmacokinetics in early drug discovery
requires high-sensitivity and exceptional robustness
The EVOQ triple quadrupole for liquid chromatography (LC-TQ) was to analyze hundreds of rat plasma samples on a
daily basis; a task easily achieved by the oriice/IQ
designed for a singular purpose – to reliably quantify thousands dual ion funnel design.
of real samples in the fastest sample-to-report time possible.
It delivers exceptional sensitivity, precision, accuracy, linearity,
and a wide dynamic range for your multiple reaction monitoring
(MRM) assays. Innovations in software and atmospheric pressure
ionization (API) technology make it a game changer for routine
high-sensitivity, quantitative analysis.
Key Benefits

Easily obtain ultra-high sensitivity for small molecules
and biomolecules as a result of the innovative interlaced-
quadrupole (IQ) dual ion funnel.
Confidently run matrix-rich samples on the robust oriice-
plate based API interface.
Efficiently analyze thermally labile molecules at high low
rates with the novel vacuum insulated probe (VIP) heated-
electrospray probe.
Save time using exception-based data-review software,
which makes it easier to highlight chromatograms that do
not meet preset method criteria.
Bruker Liquid Chromatography Solutions PRECISON, ACCURACY, SPEED,
The Advance UHPLC, HPLC, and OLE LC modules
By engineering the lowest possible system delay-volume, the Advance LC pump technology
LINEARITY, DYNAMIC RANGE
delivers superior ballistic gradient reproducibility at analytical low rates. This design-for-purpose
detail minimizes gradient delay and ensures highly precise retention times. The beneit
is shorter run-to-run cycle times with the reproducibility required for quantitative LC-MS/MS
analyses.
The OLE module extends the LC-TQ performance by enabling method-driven, on-line cleanup
or sample pre-concentration. The addition of a third pump in the same module and under full
software control reduces bench space requirement and simpliies setup, while giving you the
lexibility of rapid method development.
Sensitivity & Robustness
Your assay demands both because real samples
1 have more matrix than analyte. The EVOQ API
interface was engineered to deliver sensitivity
7 6
with uncompromised robustness; requirements
that set the standards for accuracy, precision, and
2 reproducibility.
Duty cycle impacts sensitivity, accuracy, and

precision, and is deined as the time spent by the
mass analyzer measuring the target analyte. In
MRM, the duty cycle can be close to 100% for a
single component. Even with a co-eluting internal
standard, the duty cycle of a TQ-MS is much higher
than any other MS analyzer technique that requires
3
scanning of the mass range. This is the main reason
a TQ-MS remains unbeatable for high sensitivity,
absolute quantitative analysis.
8
4
Accuracy & Precision
5 Accuracy is the measure of the closeness of the
experimental value to the actual amount of the target
analyte in matrix. It is a measure of systematic bias.
1 Highly efficient VIP heated electrospray and APCI probes standard Precision is how well the experimental values agree with
each other and is a measure of random error.
2 Superior MRM sensitivity lens-free mass analyzer
3 Flat-tuning, tune-free, IQ dual ion funnel Together, these parameters measure the total
error of the assay, which in turn determines the limit of
4 Available in high performance QubeTM or ultra performance EliteTM models quantitation (LOQ). Since the MRM technique spends
5 UHPLC, HPLC, or OLE models with built-in column oven and degasser nearly all the time measuring the target analyte, the
result is an unbeatable LOQ in matrix; something you
6 eVol digital syringe standard infusion or loop injection experiments will routinely achieve on the EVOQ LC-TQ.
7 CTC Autosampler (industry workhorse) with reduced carryover capability
8 High-capacity, cooled-tray sample storage standard
THE SCIENCE OF SENSITIVITY
Delivering Heat Where It Belongs Tune-free Ion Optics
Simply set the nebulizer temperature and let VIP technology take An elegantly simple design that captures ions in the free-jet expansion
over the responsibility of ensuring precise heated the electrospray region and eficiently focuses them into a coherent ion beam to
ionization (ESI) for maximum sensitivity. VIP, a patent-pending heated deliver high sensitivity and robustness without the need for
ESI design that eficiently ionizes thermally labile molecules such as compound-speciic tuning.
N-oxides, glucuronides, or peptides during the high low rate heated
ESI process. This innovative technology provides an insulated low
path for the LC eluent shielding it from undesirable temperature Detector The IQ dual ion funnel system produces
luctuations occurring during the LC gradient. two key benefits:
This innovative detector design (US Patent 7855361)
eliminates the need for a conversion dynode which 1. Ease-of-use, due to the funnel-shaped, RF-only focusing ield design
allows for highly sensitive negative ion detection and which concentrates the ions into a coherent ion beam, eliminating the
fast switching capability. Lens-free Design need for compound-dependent tuning.
Covered by US Patent 6576897, this unique feature improves 2. Dramatically improves instrument up-time and eliminates the need
High-efficiency ceramic MRM sensitivity by signiicantly reducing ion scattering for frequent maintenance due to the spatial geometry of the IQ
heater technology, with losses in the quadrupoles before and after the collision cell. dual ion funnel which improves removal of background gases over
configured thermal profiling,
traditional stacked-lens funnels, reducing surface contamination.
delivers highest heat near
the probe tip.
Shape vacuumTM
insulation protects the LC
eluent from boiling before
nebulization.
Orifice Interface
The EVOQ was designed to be highly sensitive, robust, and simple to operate with low
Fluid path remains cool maintenance requirements. The oriice design ensures that the ions traverse the opening into the
before nebulization, vacuum chamber and come under the inluence of the IQ dual ion funnel system in the fastest
which is essential for time possible. A hot counter-current drying gas (cone gas) guards against stray droplets entering
protecting thermally fragile the vacuum chamber. The vital requirement for high-sensitivity is simply to minimize the time
compounds.
spent going from the spray chamber into the IQ dual ion funnel in the irst vacuum region. The
oriice design delivers this simple requirement most effectively.
PERFORMANCE- PACER SOFTWARE
DRIVEN SOFTWARE
Are You Drowning in Data? Exception-based Data Review

For years your vendor kept delivering hardware that promised to run more samples per The powerful PACER integration algorithms use pattern-recognition technology
day, using automated sample preparation and ballistic gradients. Yet, your productivity to accurately deine and integrate small peaks within noisy baselines, a
did not increase. Why? They forgot that the vital process of data analysis and review challenge that increases as you approach the LOQ. By accurately picking
was still dependent on redundant levels of human interaction to ensure data quality. peaks on the large majority of components, the human workload for review
They simply shifted the bottleneck – they failed to satisfy your need for increased and manual integration is dramatically reduced. This is important because the
eficiency. Hardware is only half the solution and their solution left you drowning in highlighted chromatograms are the ones that inluence assay quality and your
data. reputation.
Imagine an innovation in software that makes the human interaction with the process In a commercial testing laboratory, we understand time is critical and data
of data review more eficient by eliminating your data review bottleneck. How? By quality is paramount. PACER software and the EVOQ LC-TQ enable you to run
employing exception-based data review to instantly highlight intergrated peaks that more samples without the typical data analysis bottlenecks. Even better, you put
fail to meet the preset method criteria, the analyst can now focus on the problem a smile on the face of your QC reviewer. Now that’s a game changer!
areas. This Bruker software innovation instantly increases eficiency by eliminating
unproductive time spent on data review without compromising data quality.
Bottlenecks eliminated from sample-to-report. Now that’s a game changer!
Time
CHALLENGE THE
STATUS QUO
Demand a better solution for LC-MRM quantitative
analysis - experience the Bruker EVOQ LC-TQ solution.
For over 50 years Bruker has embodied innovation with integrity as demonstrated by its development of
world-class scientiic instruments. Today we are pioneering the migration of technology from research to
the commercial laboratory. The EVOQ Elite and the EVOQ Qube relect this design philosophy and solve
the hardware and software challenges faced by the quantitative analysis community.
Experience the game changing performance of the EVOQ LC-TQ; experience the innovation from Bruker.
to change specifications without notice. © BDAL 08-2012. #703117
www.bruker.com

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SCION™ GC Series

The Gas Chromatographers Choice for Separations

Local User Interface Fast, Flexible Detection

Choice of multi inlet systems

Choice of multi inlet systems

Choice of 7 GC Choice of MS detector

Bruker offers a range SCION GCs to meet virtually all application

GC Control From Anywhere Turnkey Analyzer Solutions

Speed increased with a factor 6.5

Fast Cycle Time

switching are valuable techniques in

Heart cut using the Deans switch

Deans switching enables the use of

Bruker offers a range of differing injector

Injector Selection Guide - Sample/Analysis Characteristics or Requirements

✓ Capillary, 0.1 to 0.53 mm ID Split/Splitless Large Volume (LV) SS Mode

✓ ✓ Capillary, 0.53 mm ID Large Volume (LV)

✓ Capillary, retention gap Cold On-Column Large Volume PTV Mode

✓ Capillary, 0.53 mm ID Packed Large Volume (LV) PTV Mode

✓ Capillary, 0.53 mm ID Cold On-Column Large Volume (LV) On-Column Mode

Super Clean™ Gas Filters

For research use only. Not for use in diagnostic procedures.

Dimensions and Weights

Cool down rate: 400 °C to 50 °C in 4.5 minutes

For research use only. Not for use in diagnostic procedures.

Synchronization signals with other devices and data systems:

Data Handling and System Control:

For research use only. Not for use in diagnostic procedures.

S/SL Split/Splitless Injector

Suited for columns:

COC Cold On-Column Injector

Maximum temperature: 450 °C

Suited for columns:

PTV Programmable Temperature

Maximum temperature ramp rate: 200 °C/min

For research use only. Not for use in diagnostic procedures.

Suited for columns:

Maximum temperature: 450 °C

PWOC Packed/Wide-bore On-Column Injector

Suited for columns:

Electronic Flow Control: Injectors (EFC)

For research use only. Not for use in diagnostic procedures.

FID Flame Ionization Detector

Flame tip type: ceramic (patented)

TCD Thermal Conductivity Detector

ECD Electron Capture Detector

NPD (TSD) Nitrogen-Phosphorus Detector

Linear dynamic range:

For research use only. Not for use in diagnostic procedures.

Linear dynamic range:

PDHID Pulsed Discharge Helium Ionization Detector

CP-8410 Auto Injector

Pre-programmed modes of injection Syringes:

For research use only. Not for use in diagnostic procedures.

PAL Combi-xt AutoSampler

SHS-40 Headspace Sampler

For research use only. Not for use in diagnostic procedures.

Sample Capacity Up to 40 vials; 20mL

Injection Modes Fixed Sample Volume

Injection Valve 6 Way Electric Actuated

SHS-40 Incubator Up to 12 Samples

Instrument Control Remote with Compass CDS

Sample Recognition Automated