Varian, Inc.

2700 Mitchell Drive

Walnut Creek, CA 94598-1675/USA

Waters 2487
Dual Wavelength Detector
Control

Galaxie Driver Manual

©Varian, Inc. 2005 03-914947-40: Rev 1

 Table of Contents
Introduction.............................................................................................. 2
General ........................................................................................................................... 2
Installation and Configuration................................................................ 3
Installing the Driver Software .......................................................................................... 3
Configuring the Communication Server.......................................................................... 3
Configuring the driver...................................................................................................... 4
Configuring the 2487 Dual Wavelength Detector........................................................ 4
Configuring the Synopsis ............................................................................................ 6
Building a Method ................................................................................... 9
Creating a New Method .................................................................................................. 9
Control Method for the Waters 2487 Detector .............................................................. 11
Settings window......................................................................................................... 12
Starting and Viewing an Acquisition.................................................... 14
Quick Start and Sequence ............................................................................................ 14
Quick Start (Single Acquisition)................................................................................. 14
Sequence .................................................................................................................. 16
Running Acquisition Status ........................................................................................... 16
Viewing a Running Acquisition. ................................................................................. 16
Status of the Waters 2487 Detector .......................................................................... 17

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Introduction

General
This document describes the configuration procedure of the
Waters 2487 Dual Wavelength Detector driver. It also explains
how to build a control method for this driver, how to start and
view a running acquisition and what actions are available during
an analysis.

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Installation and
Configuration

Installing the Driver Software

To install the Galaxie Drivers software refer to the Galaxie
installation Guide.

Configuring the Communication Server

To communicate with the Waters 2487 module a GPIB_Interface
or a NI488 communication bus must be configured on the
acquisition server.
The buffer parameters of the GPIB_Interface bus to
communicate with the module must be the following:
TX Buffer: 1024
RX Buffer: 1024

NOTE: For more details about the configuration of a GPIB_Interface or a NI488 bus,
refer to the Galaxie Chromatography Data System Installation’s Guide.

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Configuring the Driver
The driver software configuration is done in the Galaxie
Configuration Manager.

Configuring the 2487 Dual Wavelength Detector

Click on the button corresponding to the 2487 Dual

Wavelength Detector driver and the configuration screen
appears.

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 Configuration Window

HPIB Interface Parameter Group:

In the HPIB Hardware field, select the name of the bus
previously configured with the Communication Server
Configuration tool.
In the Instrument Address field, enter the HPIB address(es) of
the instrument.
Module Start Parameter Group:
In this field, enter the type of start of the instrument: Internal if
Galaxie sends the start order to the module or External if the
module is started by remote cables.

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NOTE: To communicate with the detector, it must be configured as a dual 486. To set
this parameter, click on ‘shift’+’configure’ on the instrument front panel, then
check the Enable dual 486 box by pressing the ‘1’ button. The two GPIB
addresses are then displayed on the screen.

It is then mandatory to select the dual wavelength option of the instrument by

pressing the ‘shift’+’λ/λλ’ buttons, otherwise the second channel is ‘offline’ in
Galaxie.

Configuring the Synopsis

In the Overview part of the system configuration, a synopsis
must be defined to the system. This synopsis depends on the
configured devices.
1. To save the configuration of these devices, click on the OK
button.
2. Right click on the system name in the bottom part of the
Galaxie Configuration Manager screen. A pop-up menu
appears: click on Stop. Then right click on the system name
again, and click on Start.

3. Go back to the system configuration screen (Properties

wizard). A graphic of the devices that comprise the system is
displayed in the synopsis screen. Note that flow proceeds
from left to right. To configure the synopsis to reflect the real
instrument configuration, move each module then, connect it
appropriately. To do this, select one anchor for each of the
two modules (the selected anchor becomes red) and click on

the button. A connection tube will appear between the

two anchors.

Click on the to delete a selected tube, click on the

button to delete all the tubes.

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 Click on to define a background color, or on to
make the background transparent.

Click on the icon to change the grid color.

4. Once the synopsis configuration is complete, it will be
displayed in the Galaxie Chromatography Data System, in
the Systems tab. In this tab, the Overview will show the
synopsis of the system with some parameters displayed
(temperature, flow, signal, etc.) depending on the devices
installed.
For example, the following pictures represent the synopsis of
the Waters 2487:

Galaxie Configuration Manager Screen

Galaxie Chromatography Data System Screen

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NOTE: Once the system has been created and correctly configured, it must be
associated with one or more projects in order to start acquisitions. For more
details about the creation and configuration of a system, refer to the Galaxie
Configuration Manager User’s Guide.

Press the button to print the current configuration.

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Building a Method

Creating a New Method

The method contains parameters for instrument control, data
acquisition, chromatogram processing, and editing of final
results. To initially set up a method, it is not necessary to define
all of the sub-method sections; however, the instrument control
section must be filled in prior to starting an acquisition.
1. To create a new method, select the FILE / NEW METHOD
option from the Galaxie Chromatography Data System main
menu.
A wizard guide will appear and provide assistance during the
first steps of method creation:

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 2. Choose the system (chromatograph) associated with the
method. A method is created for a particular system. When
starting an acquisition, the name of the system that performs
the acquisition must be correct, since method access is
limited only to those methods associated with this system.
3. Once this field is completed, click on the Next button to
move to the next step of the method creation:

4. Enter the New Method Name in the first field. In the

Description field, enter information concerning the method
(optional).
5. Once these two fields have been completed, click on the OK
button. The new method is created and is opened with
default parameters in the Galaxie Chromatography Data
System. The next step is the definition of each of the method
sections.
The instrument control method section must be defined in
order to start an acquisition. In the following part of this
guide, only the control section for the Waters 2487 Detector
is detailed. Other parameters such as Injection Volume, Vial
and Rack number may be necessary and are defined in the
acquisition section.

NOTE: For more details about the programming of each method section, refer to the
Galaxie Chromatography Data System User’s Guide.

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Waters 2487 Detector Control Method
Once the new method is created, select the control section in the
lower part of the browser.
The following screen appears:

Press the button to display the System Control

Method Advanced Tools window:
to send the current method to the
instrument (not applicable to this driver)
to get the active method from the
instrument (not applicable to this driver)
to print the current method

Click on the button to display the Waters 2487 control

method section.

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Settings Window

Signal 1 and Signal 2 Parameter Groups:

Wavelength: Enter the initial wavelength of the first/second
channel.
Filter Time: Select a time constant in the drop/down list: 0.0, 0.5,
1.0, 2.0, 3.0, 4.0 and 5.0 seconds are available.
AUFS: Set the sensitivity range parameter by choosing a value
in the list: 0.001, 0.002, 0.005, 0.010, 0.020, 0.050, 0.100, 0.200,
0.500, 1.000 and 2.000 AUFS are available.
Polarity: set the polarity of the signal.

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 Time Program 1 and Time Program 2 Parameters Groups:
A time program can be defined enabling to change one of the
first 3 parameters values as a function of time:
Use the following buttons to edit the table:

to add a line to the table.

to delete a line in the table.

to clear the entire table.

Other Parameter Groups:
The signals can be set to zero at the beginning of a run if the
Autozero at Prerun box is checked.
The lamp can be turned off automatically at the end of a
quickstart or a sequence if the Automatic Lamp Stop box is
checked.
Digital Output Parameter Groups:
The Digital Data Rate can be set by selecting a value in a
drop/down list. The following values are available: 1, 2, 5 and 10
pts/s.

NOTE: If the detector is configured in dual wavelength mode, the instrument limits this
data rate to 1pt/s. If a different value is entered in this field, it will not be taken
into account for the acquisition.

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Starting and Viewing an
Acquisition

Quick Start and Sequence

Once the instrument control method has been correctly
programmed, an analysis can be started. The next section briefly
describes how to start an acquisition.

Quick Start (Single Acquisition)

1. Press the button.
In the Quick Start window, select the system, the method,
and optionally the project, if connected in "All project" mode.
2. Press the OK button
The following screen is displayed:

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 In the Acquisition parameters tab, required fields like the
Run Name, the Run ID and the Acquisition length must be
filled in before starting an analysis. Furthermore, if the
system is equipped with an autosampler controlled by the
Galaxie Chromatography Data System, the Injection
Volume, the Vial position and the Rack number (if required)
must be entered.
Other parameters fitted to each acquisition channel can be
entered in the Channel X tab.
3. Click on the Start button to start the acquisition if the system
is a single-injector system or click on the Next button if the
system is a multiple injector system. In a multiple injector
system, the same parameters entered for the first injector
must be entered for the other injectors before starting the
acquisition.

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Sequence
1. To create a sequence select the FILE / NEW SEQUENCE
menu and complete the displayed creation wizard screen
(refer to the Galaxie Chromatography Data System User’s
Guide), by entering the system name that will perform the
analysis, the number of lines (corresponding to the number
of samples) and the sequence name.
2. The sequence is displayed. Complete the sequence
information columns (refer to the Galaxie Chromatography
Data System User’s Guide).

The Method Name, the Run Name, the Run ID and the
Acquisition length must be filled in before starting the
analysis. This information is automatically imported from the
acquisition part of the method if defined.

3. Start the sequence by clicking on the button.

NOTE: For more details about how to start an acquisition, refer to the Galaxie
Chromatography Data System User’s Guide.

Running Acquisition Status

Viewing a Running Acquisition
To view a running acquisition, click on the Systems tab then
select the running system. A window is activated which is
composed of two parts: the running chromatogram and the
status window where the driver modules are listed.

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Status of the Waters 2487 Detector

Click on the button corresponding to the Waters 2487

driver, and the detector status screens will appear.

The button turns on all the modules that compose

the system.

The button turns off all the modules that compose

the system.

The button turns the lamp on.

The button turns the lamp off.

The button enables to unlock the instrument.

Indeed, when Galaxie takes the control of the detector,
the keyboard gets locked and no change can be done
through the front panel screen.
Once the modification has been done on the instrument,

it can be re-locked by pressing on the button.

The button enables to perform an

autozero of the signals.

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 The button enables to
change the wavelength of the corresponding channel.
Click on this button, the following screen appears:

Enter the new value and update the instrument by

clicking on the OK button.

Overview Window

Click on the button: the Status Overview window is

displayed and contains three tabs:
• General: displays the synopsis of the instrument, where
the current wavelength value can be read.

The button turns the lamp on.

The button turns the lamp off.

The button performs an autozero of the signals.

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 • Not ready: contains the not ready information sent by the
instrument.
• Events: contains the events that have occurred on the
instrument.

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