Professional Documents
Culture Documents
Lesson 19. Basic Principles of Radioactive Measurements (376 KB)
Lesson 19. Basic Principles of Radioactive Measurements (376 KB)
Biochemistry
19
Notes
BASIC PRINCIPLES OF
RADIOACTIVE MEASUREMENTS
19.1 INTRODUCTION
Radioactivity is defined as the process in which unstable atomic nuclei loses
energy by emitting radiation in the form particles or electromagnetic waves.
These radiations are able to ionize the atoms and molecules along their track.
These radiations are able to cause cancer and death. Therefore these radiations
are of health and safety concern.
OBJECTIVES
After reading this lesson, you will be able to:
z describe Atom
z explain radioactive delay
z describe units of radioactivity
z explain the characteristics of radioactive emissions
19.2 ATOM
An atom is defined as the smallest component of an element having the chemical
properties of the element. It consists of positively charged nucleus surrounded
by negatively charged electrons. The nucleus is made up of positively charged
protons and uncharged neutrons.
BIOCHEMISTRY 247
MODULE Basic Principles of Radioactive Measurements
Biochemistry
Notes
248 BIOCHEMISTRY
Basic Principles of Radioactive Measurements MODULE
1. Decay by negatron emission Biochemistry
BIOCHEMISTRY 249
MODULE Basic Principles of Radioactive Measurements
250 BIOCHEMISTRY
Basic Principles of Radioactive Measurements MODULE
Nt is the number of radioactive atoms present at time t,and N0 is the number Biochemistry
of radioactive atoms present originally. In practice decay constant is expressed
in terms of half life t1/2.
The half-life of an radioactive material is defined as the time taken to become
half of its original value
So Nt/No becomes ½ at t becomes t1/2. Now the above said equation becomes
Notes
Ln1/2 = - λt1/2
1
0.9
Amount of Radioactive Nuclel
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
First t½ Second t½ Third t½ Four t½
Time
Isotopes Half-life
3H 12.26 years
14C 5760 years
32P 14.20 days
35S 87.20 days
125I 60 days
BIOCHEMISTRY 251
MODULE Basic Principles of Radioactive Measurements
Biochemistry
19.4 UNITS OF RADIOACTIVITY
The radioactivity of a substance can be measured using different units. They are
z Becquerel is the SI unit for measurement of radioactivity. It is defined as
the number of disintegration per second (d.p.s.)
1 Becquerel (Bq) = 1 dps
Notes Terra Bq = 1012 Bq
Giga Bq = 109 Bq
Mega Bq = 106 Bq
z Curie is the commonly used unit. It is defined as the quantity of radioactive
material having nuclear disintegration similar to that of 1g of radium i.e.
3.7 × 1010 dps (or 37 Giga Bq)
1 Curie (Ci) = 3.7 × 1010 dps
Milli Ci = Ci × 10-3
Micro Ci = Ci × 10-6
z Specific activity of a substance is defined as activity per unit weight or
volume (e.g., Bq/gm or B/l).
z Counts per minute (c.p.m) is disintegration detected by a radiation counter.
19.5.2 Negatrons
z Negatrons are very small and rapidly moving particles
z They also cause excitation and ionization but lesser than alpha particle
z They have more penetrating power
z These are low energy particles (0.018-4.81 Mev)
252 BIOCHEMISTRY
Basic Principles of Radioactive Measurements MODULE
19.5.3 Gamma Rays Biochemistry
Notes
BIOCHEMISTRY 253
MODULE Basic Principles of Radioactive Measurements
Biochemistry
TERMINAL QUESTIONS
1. What is Radioactivity?
2. What are the different types of radioactive decay?
3. What is half-life?
Notes 4. What are the characteristics of various radioactive emissions?
5. Write a note on units of radioactivity.
18.1
1. 1. (a) 2. (b) 3. (c) 4. (d) 5. (e)
2. 1. F 2. F 3. F 4. F 5. F
254 BIOCHEMISTRY